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  1. The Saccharopolyspora erythraea genome sequence, recently published, presents considerable divergence from those of streptomycetes in gene organization and function, confirming the remarkable potential of S. eryt...

    Authors: Clelia Peano, Silvio Bicciato, Giorgio Corti, Francesco Ferrari, Ermanno Rizzi, Raoul JP Bonnal, Roberta Bordoni, Alberto Albertini, Luigi Rossi Bernardi, Stefano Donadio and Gianluca De Bellis
    Citation: Microbial Cell Factories 2007 6:37
  2. Human α1-proteinase inhibitor (α1-PI), also known as antitrypsin, is the most abundant serine protease inhibitor (serpin) in plasma. Its deficiency is associated with development of progressive, ultimately fatal ...

    Authors: Elena Karnaukhova, Yakir Ophir, Loc Trinh, Nimish Dalal, Peter J Punt, Basil Golding and Joseph Shiloach
    Citation: Microbial Cell Factories 2007 6:34
  3. The yeast Saccharomyces cerevisiae is a well-established model system for understanding fundamental cellular processes relevant to higher eukaryotic organisms. Less known is its value for virus research, an area ...

    Authors: Rui P Galao, Nicoletta Scheller, Isabel Alves-Rodrigues, Tanja Breinig, Andreas Meyerhans and Juana Díez
    Citation: Microbial Cell Factories 2007 6:32
  4. The increasing commercial demand for L-carnitine has led to a multiplication of efforts to improve its production with bacteria. The use of different cell environments, such as growing, resting, permeabilized,...

    Authors: Vicente Bernal, Ángel Sevilla, Manuel Cánovas and José L Iborra
    Citation: Microbial Cell Factories 2007 6:31
  5. The rational design of L-phenylalanine (L-Phe) overproducing microorganisms has been successfully achieved by combining different genetic strategies such as inactivation of the phosphoenolpyruvate: phosphotran...

    Authors: José Luis Báez-Viveros, Noemí Flores, Katy Juárez, Patricia Castillo-España, Francisco Bolivar and Guillermo Gosset
    Citation: Microbial Cell Factories 2007 6:30
  6. Thioredoxin (TRX) is a powerful disulfide oxido-reductase that catalyzes a wide spectrum of redox reactions in the cell. The aim of this study is to elucidate the role of the TRX system in the oxidative stress...

    Authors: L Mariela Serrano, Douwe Molenaar, Michiel Wels, Bas Teusink, Peter A Bron, Willem M de Vos and Eddy J Smid
    Citation: Microbial Cell Factories 2007 6:29
  7. Natural allergen sources can supply large quantities of authentic allergen mixtures for use as immunotherapeutics. However, such extracts are complex, difficult to define, vary from batch to batch, which may l...

    Authors: Jacob Glenting, Lars K Poulsen, Kentaro Kato, Søren M Madsen, Hanne Frøkiær, Camilla Wendt and Helle W Sørensen
    Citation: Microbial Cell Factories 2007 6:28
  8. It is now more than 10 years since the publication of the first microbial genome sequence and science is now moving towards a post genomic era with transcriptomics and proteomics offering insights into cellula...

    Authors: Robert LJ Graham, Ciaren Graham and Geoff McMullan
    Citation: Microbial Cell Factories 2007 6:26
  9. Exposure to nickel (Ni) and its chemical derivatives has been associated with severe health effects in human. On the contrary, poor knowledge has been acquired on target physiological processes or molecular me...

    Authors: Anna M Salzano, Ferdinando Febbraio, Tiziana Farias, Giovanni P Cetrangolo, Roberto Nucci, Andrea Scaloni and Giuseppe Manco
    Citation: Microbial Cell Factories 2007 6:25
  10. An important objective in developing new drugs is the achievement of high specificity to maximize curing effect and minimize side-effects, and high specificity is an integral part of the antisense approach. Th...

    Authors: Louise Carøe Vohlander Rasmussen, Hans Uffe Sperling-Petersen and Kim Kusk Mortensen
    Citation: Microbial Cell Factories 2007 6:24
  11. For many microbial processes, the complexity of the metabolisms and the responses to transient and realistic conditions are difficult to capture in mechanistic models. The cells seem to have an innate intellig...

    Authors: Pratap R Patnaik
    Citation: Microbial Cell Factories 2007 6:23
  12. Human immune cells generate large amounts of reactive oxygen species (ROS) throughout the respiratory burst that occurs during inflammation. In inflammatory bowel diseases, a sustained and abnormal activation ...

    Authors: Tatiana Rochat, Luis Bermúdez-Humarán, Jean-Jacques Gratadoux, Christel Fourage, Christine Hoebler, Gérard Corthier and Philippe Langella
    Citation: Microbial Cell Factories 2007 6:22
  13. The analysis of transcriptional levels of the genes involved in protein synthesis and secretion is a key factor to understand the host organism's responses to recombinant protein production, as well as their i...

    Authors: David Resina, Mónika Bollók, Narendar K Khatri, Francisco Valero, Peter Neubauer and Pau Ferrer
    Citation: Microbial Cell Factories 2007 6:21
  14. Streptokinase (SK) is a potent plasminogen activator with widespread clinical use as a thrombolytic agent. It is naturally secreted by several strains of beta-haemolytic streptococci. The low yields obtained i...

    Authors: Elsa Pimienta, Julio C Ayala, Caridad Rodríguez, Astrid Ramos, Lieve Van Mellaert, Carlos Vallín and Jozef Anné
    Citation: Microbial Cell Factories 2007 6:20
  15. Corynebacterium glutamicum has several anaplerotic pathways (anaplerosis), which are essential for the productions of amino acids, such as lysine and glutamate. It is still not clear how flux changes in anaplerot...

    Authors: Tomokazu Shirai, Koki Fujimura, Chikara Furusawa, Keisuke Nagahisa, Suteaki Shioya and Hiroshi Shimizu
    Citation: Microbial Cell Factories 2007 6:19
  16. Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal intestinal flora is ...

    Authors: Asgar Ergin, Konrad Büssow, Joachim Sieper, Andreas Thiel, Rainer Duchmann and Thomas Adam
    Citation: Microbial Cell Factories 2007 6:18
  17. Commercial medium (Pharmamedia) was investigated for the production of surfactin by Bacillus subtilis MZ-7. Different media (defined, semi-defined, and complex media) were compared for the production of surfactin...

    Authors: Muaaz Mutaz Al-Ajlani, Muhammad Abid Sheikh, Zeeshan Ahmad and Shahida Hasnain
    Citation: Microbial Cell Factories 2007 6:17
  18. Despite the fact that many reports deal with glycolysis in Lactococcus lactis, there is not much information on the regulation of uptake of glucose itself. The aim of the present work was to investigate the effec...

    Authors: Maria Papagianni, Nicholaos Avramidis and George Filiousis
    Citation: Microbial Cell Factories 2007 6:16
  19. Together with the development of optical sensors, fluorometry is becoming an increasingly attractive tool for the monitoring of cultivation processes. In this context, the green fluorescence protein (GFP) has ...

    Authors: Anna Surribas, David Resina, Pau Ferrer and Francisco Valero
    Citation: Microbial Cell Factories 2007 6:15
  20. Vanillin is one of the most important aromatic flavour compounds used in the food and cosmetic industries. Natural vanillin is extracted from vanilla beans and is relatively expensive. Moreover, the consumer d...

    Authors: Paolo Barghini, Diana Di Gioia, Fabio Fava and Maurizio Ruzzi
    Citation: Microbial Cell Factories 2007 6:13
  21. Lactic acid bacteria (LAB) are attractive tools to deliver therapeutic molecules at the mucosal level. The model LAB Lactococcus lactis has been intensively used to produce and deliver such heterologous proteins....

    Authors: Stéphane Hazebrouck, Laetitia Pothelune, Vasco Azevedo, Gérard Corthier, Jean-Michel Wal and Philippe Langella
    Citation: Microbial Cell Factories 2007 6:12
  22. All organisms living under aerobic atmosphere have powerful mechanisms that confer their macromolecules protection against oxygen reactive species. Microorganisms have developed biomolecule-protecting systems ...

    Authors: Ebert S Hanna, Maria-Cristina Roque-Barreira, Emerson S Bernardes, Ademilson Panunto-Castelo, Marcelo V Sousa, Igor C Almeida and Marcelo Brocchi
    Citation: Microbial Cell Factories 2007 6:11
  23. Saccharomyces cerevisiae is widely utilized in basic research as a model eukaryotic organism and in biotechnology as a host for heterologous protein production. Both activities demand the use of highly regulated ...

    Authors: María J Quintero, Douglas Maya, Miguel Arévalo-Rodríguez, Ángel Cebolla and Sebastián Chávez
    Citation: Microbial Cell Factories 2007 6:10
  24. Several aspects of alginate and PHB synthesis in Azotobacter vinelandii at a molecular level have been elucidated in articles published during the last ten years. It is now clear that alginate and PHB synthesis a...

    Authors: Enrique Galindo, Carlos Peña, Cinthia Núñez, Daniel Segura and Guadalupe Espín
    Citation: Microbial Cell Factories 2007 6:7
  25. Fluxome analysis aims at the quantitative analysis of in vivo carbon fluxes in metabolic networks, i. e. intracellular activities of enzymes and pathways. It allows investigating the effects of genetic or envi...

    Authors: Christoph Wittmann
    Citation: Microbial Cell Factories 2007 6:6
  26. Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomer...

    Authors: Kaisa Karhumaa, Rosa Garcia Sanchez, Bärbel Hahn-Hägerdal and Marie-F Gorwa-Grauslund
    Citation: Microbial Cell Factories 2007 6:5
  27. With advances in robotics, computational capabilities, and the fabrication of high quality glass slides coinciding with increased genomic information being available on public databases, microarray technology ...

    Authors: Pratik Jaluria, Konstantinos Konstantopoulos, Michael Betenbaugh and Joseph Shiloach
    Citation: Microbial Cell Factories 2007 6:4
  28. P. multocida is a Gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. Current vaccines include bacterins, which provide only l...

    Authors: Keith Al-Hasani, John Boyce, Victoria P McCarl, Stephen Bottomley, Ian Wilkie and Ben Adler
    Citation: Microbial Cell Factories 2007 6:3
  29. Recombinant antibodies are essential reagents for research, diagnostics and therapy. The well established production host Escherichia coli relies on the secretion into the periplasmic space for antibody synthesis...

    Authors: Eva Jordan, Michael Hust, Andreas Roth, Rebekka Biedendieck, Thomas Schirrmann, Dieter Jahn and Stefan Dübel
    Citation: Microbial Cell Factories 2007 6:2
  30. Recombinant proteins expressed in Escherichia coli vectors are generally contaminated with endotoxin. In this study, we evaluated the ability of Polymyxin B to neutralize the effect of LPS present as contaminant ...

    Authors: Luciana S Cardoso, Maria Ilma Araujo, Alfredo M Góes, Lucila G Pacífico, Ricardo R Oliveira and Sergio C Oliveira
    Citation: Microbial Cell Factories 2007 6:1
  31. The β-galactosidase from Kluyveromyces lactis is a protein of outstanding biotechnological interest in the food industry and milk whey reutilization. However, due to its intracellular nature, its industrial produ...

    Authors: Ángel Pereira Rodríguez, Rafael Fernández Leiro, M Cristina Trillo, M Esperanza Cerdán, M Isabel González Siso and Manuel Becerra
    Citation: Microbial Cell Factories 2006 5:41
  32. The final aim of recombinant protein production is both to have a high specific production rate and a high product quality. It was already shown that using cold-adapted bacteria as host vectors, some "intracta...

    Authors: Angela Maria Cusano, Ermenegilda Parrilli, Gennaro Marino and Maria Luisa Tutino
    Citation: Microbial Cell Factories 2006 5:40
  33. Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing nu...

    Authors: Franz S Hartner and Anton Glieder
    Citation: Microbial Cell Factories 2006 5:39
  34. Messenger RNA decay is an important mechanism for controlling gene expression in all organisms. The rate of the mRNA degradation directly affects the steady state concentration of mRNAs and therefore influence...

    Authors: Christian Kemmer and Peter Neubauer
    Citation: Microbial Cell Factories 2006 5:38
  35. Secretion of heterologous proteins depends both on biomass concentration and on the specific product secretion rate, which in turn is not constant at varying specific growth rates. As fed batch processes usual...

    Authors: Michael Maurer, Manfred Kühleitner, Brigitte Gasser and Diethard Mattanovich
    Citation: Microbial Cell Factories 2006 5:37
  36. During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. megaterium ATCC14945 penicilli...

    Authors: Yang Yang, Rebekka Biedendieck, Wei Wang, Martin Gamer, Marco Malten, Dieter Jahn and Wolf-Dieter Deckwer
    Citation: Microbial Cell Factories 2006 5:36
  37. Lactic acid bacteria (LAB) are widely used in food industry and their growth performance is important for the quality of the fermented product. During industrial processes changes in temperature may represent ...

    Authors: Cristiana Castaldo, Rosa A Siciliano, Lidia Muscariello, Rosangela Marasco and Margherita Sacco
    Citation: Microbial Cell Factories 2006 5:35
  38. Although understanding of physiological interactions between plasmid DNA and its host is important for vector design and host optimization in many biotechnological applications, to our knowledge, global studie...

    Authors: Zhijun Wang, Li Xiang, Junjie Shao, Alicja Węgrzyn and Grzegorz Węgrzyn
    Citation: Microbial Cell Factories 2006 5:34
  39. Yeasts provide attractive expression platforms in combining ease of genetic manipulation and fermentation of a microbial organism with the capability to secrete and to modify proteins according to a general eu...

    Authors: Gerhard Steinborn, Erik Böer, Anja Scholz, Kristina Tag, Gotthard Kunze and Gerd Gellissen
    Citation: Microbial Cell Factories 2006 5:33
  40. Industrial fermentation typically uses complex nitrogen substrates which consist of mixture of amino acids. The uptake of amino acids is known to be mediated by several amino acid transporters with certain pre...

    Authors: Prashant M Bapat, Debasish Das, Sujata V Sohoni and Pramod P Wangikar
    Citation: Microbial Cell Factories 2006 5:32
  41. Fungal laccases are useful enzymes for industrial applications; they exhibit broad substrate specificity and thus are able to oxidize a variety of xenobiotic compounds including chlorinated phenolics, syntheti...

    Authors: Maria Chiara Colao, Stefania Lupino, Anna Maria Garzillo, Vincenzo Buonocore and Maurizio Ruzzi
    Citation: Microbial Cell Factories 2006 5:31

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