Articles

Metabolic flux sampling predicts strain-dependent differences related to aroma production among commercial wine yeasts

Metabolomics coupled with genome-scale metabolic modeling approaches have been employed recently to quantitatively analyze the physiological states of various organisms, including Saccharomyces cerevisiae. Althou...

Authors: William T. Scott Jr., Eddy J. Smid, David E. Block and Richard A. Notebaart

OsnR is an autoregulatory negative transcription factor controlling redox-dependent stress responses in Corynebacterium glutamicum

Corynebacterium glutamicum is used in the industrial production of amino acids and nucleotides. During the course of fermentation, C. glutamicum cells face various stresses and employ multiple regulatory genes to...

Authors: Haeri Jeong, Younhee Kim and Heung-Shick Lee

A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae

Saccharomyces cerevisiae is an important synthetic biology chassis for microbial production of valuable molecules. Promoter engineering has been frequently applied to generate more synthetic promoters with a vari...

Authors: Jiliang Deng, Yanling Wu, Zhaohui Zheng, Nanzhu Chen, Xiaozhou Luo, Hongting Tang and Jay D. Keasling

Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system

Autoinduction systems can regulate protein production in Escherichia coli without the need to monitor cell growth or add inducer at the proper time following culture growth. Compared to classical IPTG induction, ...

Authors: Qin Fan, Peter Neubauer and Matthias Gimpel

Establishment of a co-culture system using Escherichia coli and Pichia pastoris (Komagataella phaffii) for valuable alkaloid production

Plants produce a variety of specialized metabolites, many of which are used in pharmaceutical industries as raw materials. However, certain metabolites may be produced at markedly low concentrations in plants....

Authors: Miya Urui, Yasuyuki Yamada, Yoshito Ikeda, Akira Nakagawa, Fumihiko Sato, Hiromichi Minami and Nobukazu Shitan

Non-destructive quantification of anaerobic gut fungi and methanogens in co-culture reveals increased fungal growth rate and changes in metabolic flux relative to mono-culture

Quantification of individual species in microbial co-cultures and consortia is critical to understanding and designing communities with prescribed functions. However, it is difficult to physically separate spe...

Authors: Patrick A. Leggieri, Corey Kerdman-Andrade, Thomas S. Lankiewicz, Megan T. Valentine and Michelle A. O’Malley

Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria

The production of N-linked glycoproteins in genetically amenable bacterial hosts offers great potential for reduced cost, faster/simpler bioprocesses, greater customisation, and utility for distributed manufac...

Authors: Fenryco Pratama, Dennis Linton and Neil Dixon

Degradation of epigallocatechin and epicatechin gallates by a novel tannase Tan_Hcw from Herbaspirillum camelliae

Herbaspirillum camelliae is a gram-negative endophyte isolated from the tea plant. Both strains WT00C and WT00F were found to hydrolyze epigallocatechin-3-gallate (EGCG) and epicatechin-3-gallate (ECG) to release...

Authors: Jia Lei, Yong Zhang, Xuechen Ni, Xuejing Yu and Xingguo Wang

Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis

Glycerol is a desirable alternative substrate for 2,3-butanediol (2,3-BD) production for sustainable development in biotechnological industries and non-food competitive feedstock. B. subtilis, a “generally recogn...

Authors: Nunthaphan Vikromvarasiri, Tomokazu Shirai and Akihiko Kondo

High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast

Contemporary biotechnology focuses on many problems related to the functioning of developed societies. Many of these problems are related to health, especially with the rapidly rising numbers of people sufferi...

Authors: Magdalena Rakicka-Pustułka, Joanna Miedzianka, Dominika Jama, Sylwia Kawalec, Kamila Liman, Tomasz Janek, Grzegorz Skaradziński, Waldemar Rymowicz and Zbigniew Lazar

Heterologous production of novel and rare C₃₀-carotenoids using Planococcus carotenoid biosynthesis genes

Members of the genus Planococcus have been revealed to utilize and degrade solvents such as aromatic hydrocarbons and alkanes, and likely to acquire tolerance to solvents. A yellow marine bacterium Planococcus ma...

Authors: Miho Takemura, Chiharu Takagi, Mayuri Aikawa, Kanaho Araki, Seon-Kang Choi, Mitsuhiro Itaya, Kazutoshi Shindo and Norihiko Misawa

Multivalent poultry vaccine development using Protein Glycan Coupling Technology

Poultry is the world's most popular animal-based food and global production has tripled in the past 20 years alone. Low-cost vaccines that can be combined to protect poultry against multiple infections are a c...

Authors: Marta Mauri, Thippeswamy H. Sannasiddappa, Prerna Vohra, Ricardo Corona-Torres, Alexander A. Smith, Cosmin Chintoan-Uta, Abi Bremner, Vanessa S. Terra, Sherif Abouelhadid, Mark P. Stevens, Andrew J. Grant, Jon Cuccui and Brendan W. Wren

Genome mining of novel rubiginones from Streptomyces sp. CB02414 and characterization of the post-PKS modification steps in rubiginone biosynthesis

Rubiginones belong to the angucycline family of aromatic polyketides, and they have been shown to potentiate the vincristine (VCR)-induced cytotoxicity against VCR-resistant cancer cell lines. However, the bio...

Authors: Jingyan Zhang, Ying Sun, Yeji Wang, Xin Chen, Lu Xue, Jingjing Zhang, Xiangcheng Zhu, Yanwen Duan and Xiaohui Yan

Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay

The split GFP assay is a well-known technology for activity-independent screening of target proteins. A superfolder GFP is split into two non-fluorescent parts, GFP11 which is fused to the target protein and G...

Authors: Carolin Müller, Chika L. Igwe, Wolfgang Wiechert and Marco Oldiges

Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation

Authors: Ram Shankar, Nina Schäfer, Marco Schmeer, Joe Max Risse, Karl Friehs and Martin Schleef

Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production

Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of...

Authors: Fei Du, Yun-Qi Liu, Ying-Shuang Xu, Zi-Jia Li, Yu-Zhou Wang, Zi-Xu Zhang and Xiao-Man Sun

Influence of B. subtilis 3NA mutations in spo0A and abrB on surfactin production in B. subtilis 168

Bacillus subtilis is a well-established host for a variety of bioproduction processes, with much interest focused on the production of biosurfactants such as the cyclic lipopeptide surfactin. Surfactin production...

Authors: Peter Klausmann, Lars Lilge, Moritz Aschern, Katja Hennemann, Marius Henkel, Rudolf Hausmann and Kambiz Morabbi Heravi

Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol

The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceut...

Authors: Seyed Hossein Helalat, Carsten Jers, Mandana Bebahani, Hassan Mohabatkar and Ivan Mijakovic

Immunization with Leishmania tarentolae-derived norovirus virus-like particles elicits high humoral response and stimulates the production of neutralizing antibodies

Noroviruses are a major cause of epidemic and sporadic acute non-bacterial gastroenteritis worldwide. Unfortunately, the development of an effective norovirus vaccine has proven difficult and no prophylactic v...

Authors: Mirosława Panasiuk, Karolina Zimmer, Anna Czarnota, Katarzyna Grzyb, Magdalena Narajczyk, Grażyna Peszyńska-Sularz, Sabina Żołędowska, Dawid Nidzworski, Lilit Hovhannisyan and Beata Gromadzka

Glycerol or crude glycerol as substrates make Pseudomonas aeruginosa achieve anaerobic production of rhamnolipids

The anaerobic production of rhamnolipids is significant in research and application, such as foamless fermentation and in situ production of rhamnolipids in the anoxic environments. Although a few studies reporte...

Authors: Feng Zhao, Yuting Wu, Qingzhi Wang, Mengyao Zheng and Qingfeng Cui

Automation assisted anaerobic phenotyping for metabolic engineering

Microorganisms can be metabolically engineered to produce a wide range of commercially important chemicals. Advancements in computational strategies for strain design and synthetic biological techniques to con...

Authors: Kaushik Raj, Naveen Venayak, Patrick Diep, Sai Akhil Golla, Alexander F. Yakunin and Radhakrishnan Mahadevan

Synthesis of (−)−deoxypodophyllotoxin and (−)−epipodophyllotoxin via a multi-enzyme cascade in E. coli

The aryltetralin lignan (−)−podophyllotoxin is a potent antiviral and anti-neoplastic compound that is mainly found in Podophyllum plant species. Over the years, the commercial demand for this compound rose notab...

Authors: Davide Decembrino, Alessandra Raffaele, Ronja Knöfel, Marco Girhard and Vlada B. Urlacher

Expression, biochemical and structural characterization of high-specific-activity β-amylase from Bacillus aryabhattai GEL-09 for application in starch hydrolysis

β-amylase (EC 3.2.1.2) is an exo-enzyme that shows high specificity for cleaving the α-1,4-glucosidic linkage of starch from the non-reducing end, thereby liberating maltose. In this study, we heterologously e...

Authors: Xuguo Duan, Qiuyu Zhu, Xinyi Zhang, Zhenyan Shen and Yue Huang

Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol

Hydroxycinnamic acids such as p-coumaric acid (CA) are chemically linked to lignin in grassy biomass with fairly labile ester bonds and therefore represent a straightforward opportunity to extract and valorize li...

Authors: Alberto Rodriguez, Jamie A. Meadows, Ning Sun, Blake A. Simmons and John M. Gladden

Optimizing operational parameters for the enzymatic production of furandicarboxylic acid building block

2,5-Furandicarboxylic acid (FDCA) is a precursor for green plastics due to its structural similarity to terephthalic acid, a common precursor of oil-derived polymers, and its potential production from sugars o...

Authors: María Isabel Sánchez-Ruiz, Angel T. Martínez and Ana Serrano

Aspergillus caespitosus ASEF14, an oleaginous fungus as a potential candidate for biodiesel production using sago processing wastewater (SWW)

Oleaginous microorganisms are sustainable alternatives for the production of biodiesel. Among them, oleaginous fungi are known for their rapid growth, short life cycles, no light requirement, easy scalability,...

Authors: Naganandhini Srinivasan, Kiruthika Thangavelu, Ashika Sekar, B. Sanjeev and Sivakumar Uthandi

Recent progress in the application of omics technologies in the study of bio-mining microorganisms from extreme environments

Bio-mining microorganisms are a key factor affecting the metal recovery rate of bio-leaching, which inevitably produces an extremely acidic environment. As a powerful tool for exploring the adaptive mechanisms...

Authors: Min Li and Jianping Wen

Functional characterization of a highly specific l-arabinose transporter from Trichoderma reesei

Lignocellulose biomass has been investigated as a feedstock for second generation biofuels and other value-added products. Some of the processes for biofuel production utilize cellulases and hemicellulases to ...

Authors: Sami Havukainen, Jonai Pujol-Giménez, Mari Valkonen, Matthias A. Hediger and Christopher P. Landowski

Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras

Monomeric autotransporters have been used extensively to transport recombinant proteins or protein domains to the cell surface of Gram-negative bacteria amongst others for antigen display. Genetic fusion of su...

Authors: Trang H. Phan, Coen Kuijl, Dung T. Huynh, Wouter S. P. Jong, Joen Luirink and Peter van Ulsen

Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous

Astaxanthin is a carotenoid with a number of assets useful for the food, cosmetic and pharmaceutical industries. Nowadays, it is mainly produced by chemical synthesis. However, the process leads to an enantiom...

Authors: Alejandro Torres-Haro, Jorge Verdín, Manuel R. Kirchmayr and Melchor Arellano-Plaza

The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis

Bacillus subtilis is one of the most important microorganisms for recombinant protein production. It possesses the GRAS (generally recognized as safe) status and a potent protein secretion capacity. Secretory pro...

Authors: Patrick Lenz, Fabienne Hilgers, Alina Burmeister, Leonie Zimmermann, Kristina Volkenborn, Alexander Grünberger, Dietrich Kohlheyer, Thomas Drepper, Karl-Erich Jaeger and Andreas Knapp

Transcriptomics reveal different metabolic strategies for acid resistance and gamma-aminobutyric acid (GABA) production in select Levilactobacillus brevis strains

Of the many neurotransmitters in humans, gamma-aminobutyric acid (GABA) shows potential for improving several mental health indications such as stress and anxiety. The microbiota-gut-brain axis is an important...

Authors: Sagarika Banerjee, Matthew Poore, Svetlana Gerdes, Derek Nedveck, Lene Lauridsen, Heidi Thomsen Kristensen, Henrik Max Jensen, Phillip M. Byrd, Arthur C. Ouwehand, Elaine Patterson and Wesley Morovic

Engineering of microbial cells for L-valine production: challenges and opportunities

L-valine is an essential amino acid that has wide and expanding applications with a suspected growing market demand. Its applicability ranges from animal feed additive, ingredient in cosmetic and special nutri...

Authors: Hui Gao, Philibert Tuyishime, Xian Zhang, Taowei Yang, Meijuan Xu and Zhiming Rao

Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification

The use of bovine-origin ribonucleases has been part of the standard protocol for plasmid DNA purification. As the field of gene therapy now enters the clinical stage, such enzymes need to be phased out or alt...

Authors: Ram Shankar, Nina Schäffer, Marco Schmeer, Joe Max Risse, Karl Friehs and Martin Schleef

Combining protein and metabolic engineering strategies for biosynthesis of melatonin in Escherichia coli

Melatonin has attracted substantial attention because of its excellent prospects for both medical applications and crop improvement. The microbial production of melatonin is a safer and more promising alternat...

Authors: Yanfeng Zhang, Yongzhi He, Nan Zhang, JiaJia Gan, Shan Zhang and Zhiyang Dong

Recombinant protein production provoked accumulation of ATP, fructose-1,6-bisphosphate and pyruvate in E. coli K12 strain TG1

Recently it was shown that production of recombinant proteins in E. coli BL21(DE3) using pET based expression vectors leads to metabolic stress comparable to a carbon overfeeding response. Opposite to original ex...

Authors: Jan Weber, Zhaopeng Li and Ursula Rinas

Improving citric acid production of an industrial Aspergillus niger CGMCC 10142: identification and overexpression of a high-affinity glucose transporter with different promoters

Glucose transporters play an important role in the fermentation of citric acid. In this study, a high-affinity glucose transporter (HGT1) was identified and overexpressed in the industrial strain A. niger CGMCC 1...

Authors: Xianli Xue, Futi Bi, Boya Liu, Jie Li, Lan Zhang, Jian Zhang, Qiang Gao and Depei Wang

Bioconversion of sago processing wastewater into biodiesel: Optimization of lipid production by an oleaginous yeast, Candida tropicalis ASY2 and its transesterification process using response surface methodology

Biodiesel is an eco-friendly and renewable energy source and a valuable substitute for petro-diesel. Sago processing wastewater (SWW), a by-product of the cassava processing industry, has starch content rangin...

Authors: Kiruthika Thangavelu, Pugalendhi Sundararaju, Naganandhini Srinivasan and Sivakumar Uthandi

Improvement of FK506 production via metabolic engineering-guided combinational strategies in Streptomyces tsukubaensis

FK506, a macrolide mainly with immunosuppressive activity, can be produced by various Streptomyces strains. However, one of the major challenges in the fermentation of FK506 is its insufficient production, result...

Authors: Qing-Bin Wu, Xiao-Ying Zhang, Xin-Ai Chen and Yong-Quan Li

Improved production of 2′-fucosyllactose in engineered Saccharomyces cerevisiae expressing a putative α-1, 2-fucosyltransferase from Bacillus cereus

2′-fucosyllactose (2′-FL) is one of the most abundant oligosaccharides in human milk. It constitutes an authorized functional additive to improve infant nutrition and health in manufactured infant formulations...

Authors: Mingyuan Xu, Xiangfeng Meng, Weixin Zhang, Yu Shen and Weifeng Liu

Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum

Ganoderma lucidum, a well-known medicinal mushroom, has received wide attention as a promising cell factory for producing bioactive compounds. However, efficient expression of heterologous genes remains a major c...

Authors: Hao You, Bin Sun, Na Li and Jun-Wei Xu

Algal glycobiotechnology: omics approaches for strain improvement

Microalgae has the capability to replace petroleum-based fuels and is a promising option as an energy feedstock because of its fast growth, high photosynthetic capacity and remarkable ability to store energy r...

Authors: Ranjna Sirohi, Jaemin Joun, Hong II Choi, Vivek Kumar Gaur and Sang Jun Sim

Combining functional metagenomics and glycoanalytics to identify enzymes that facilitate structural characterization of sulfated N-glycans

Sulfate modification of N-glycans is important for several biological functions such as clearance of pituitary hormones or immunoregulation. Yet, the prevalence of this N-glycan modification and its functions rem...

Authors: Léa Chuzel, Samantha L. Fossa, Madison L. Boisvert, Samanta Cajic, René Hennig, Mehul B. Ganatra, Udo Reichl, Erdmann Rapp and Christopher H. Taron

Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal peptides

As a robust, fast growing and genetically tractable organism, the budding yeast Saccharomyces cerevisiae is one of the most widely used hosts in biotechnology. Its applications range from the manufacturing of vac...

Authors: Anna Tippelt and Markus Nett

Production of neoagarooligosaccharides by probiotic yeast Saccharomyces cerevisiae var. boulardii engineered as a microbial cell factory

Saccharomyces cerevisiae var. boulardii is a representative probiotic yeast that has been widely used in the food and pharmaceutical industries. However, S. boulardii has not been studied as a microbial cell fact...

Authors: Yerin Jin, Sora Yu, Jing-Jing Liu, Eun Ju Yun, Jae Won Lee, Yong-Su Jin and Kyoung Heon Kim

Correction to: Refactoring of a synthetic raspberry ketone pathway with EcoFlex

Authors: Simon J. Moore, Yonek B. Hleba, Sarah Bischoff, David Bell, Karen M. Polizzi and Paul S. Freemont

Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria

4-Androstene-3,17-dione (4-AD), 1,4-androstadiene-3,17-dione (ADD) and 9α-hydroxyl-4-androstene-3,17-dione (9OH-AD), which are important starting compounds for the synthesis of steroidal medicines, can be bios...

Authors: Xin Li, Tian Chen, Fei Peng, Shikui Song, Jingpeng Yu, Douanla Njimeli Sidoine, Xiyao Cheng, Yongqi Huang, Yijun He and Zhengding Su

Bacterioferritin: a key iron storage modulator that affects strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona

Butenyl-spinosyn, produced by Saccharopolyspora pogona, is a promising biopesticide due to excellent insecticidal activity and broad pesticidal spectrum. Bacterioferritin (Bfr, encoded by bfr) regulates the stora...

Authors: Jianli Tang, Zirong Zhu, Haocheng He, Zhudong Liu, Ziyuan Xia, Jianming Chen, Jinjuan Hu, Li Cao, Jie Rang, Ling Shuai, Yang Liu, Yunjun Sun, Xuezhi Ding, Shengbiao Hu and Liqiu Xia

Screening and evaluation of the strong endogenous promoters in Pichia pastoris

Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthe...

Authors: Weiwang Dou, Quanchao Zhu, Meihua Zhang, Zuyuan Jia and Wenjun Guan

Development and optimization of a microbial co-culture system for heterologous indigo biosynthesis

Indigo is a color molecule with a long history of being used as a textile dye. The conventional production methods are facing increasing economy, sustainability and environmental challenges. Therefore, develop...

Authors: Tingting Chen, Xiaonan Wang, Lei Zhuang, Alan Shao, Yinghua Lu and Haoran Zhang