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  1. Content type: Research

    Simultaneous co-fermentation of mixed sugars is an important feature to consider in the production of ethanol from lignocellulosic biomass hydrolysates because it enhances the overall ethanol yield and volumet...

    Authors: Marco T. Fernández-Sandoval, Juvencio Galíndez-Mayer, Francisco Bolívar, Guillermo Gosset, Octavio T. Ramírez and Alfredo Martinez

    Citation: Microbial Cell Factories 2019 18:145

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  2. Content type: Research

    Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia. Recently, it has been shown to be a potentiall...

    Authors: Qi Liu, Xiaona Shi, Lili Song, Haifeng Liu, Xiangshan Zhou, Qiyao Wang, Yuanxing Zhang and Menghao Cai

    Citation: Microbial Cell Factories 2019 18:144

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  3. Content type: Research

    In most microbial cultivations d-glucose is the main carbon and energy source. However, quantification of d-glucose especially in small scale is still challenging. Therefore, we developed a FRET-based glucose bio...

    Authors: Julia Otten, Niklas Tenhaef, Roman P. Jansen, Johannes Döbber, Lisa Jungbluth, Stephan Noack, Marco Oldiges, Wolfgang Wiechert and Martina Pohl

    Citation: Microbial Cell Factories 2019 18:143

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  4. Content type: Research

    Hirame novirhabdovirus (HIRRV) can infect a wide range of marine and freshwater fish, causing huge economic losses to aquaculture industry. Vaccine development, especially oral vaccine, has become an effective...

    Authors: Lining Zhao, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing and Wenbin Zhan

    Citation: Microbial Cell Factories 2019 18:142

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  5. Content type: Research

    Bacillus subtilis 916 has been identified as an effective biocontrol agent against Rhizoctonia solani, the causal pathogen of rice sheath blight, under greenhouse and field conditions. HPLC analysis showed that s...

    Authors: Xiaoyu Wang, Zhiyi Chen, Hui Feng, Xi Chen and Lihui Wei

    Citation: Microbial Cell Factories 2019 18:141

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  6. Content type: Research

    S-Formylglutathione is hydrolyzed to glutathione and formate by an S-formylglutathione hydrolase (SFGH) ( This thiol esterase belongs to the esterase family and is also known as esterase D. SFGHs contai...

    Authors: Chang Woo Lee, Wanki Yoo, Sun-Ha Park, Ly Thi Huong Luu Le, Chang-Sook Jeong, Bum Han Ryu, Seung Chul Shin, Han-Woo Kim, Hyun Park, Kyeong Kyu Kim, T. Doohun Kim and Jun Hyuck Lee

    Citation: Microbial Cell Factories 2019 18:140

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  7. Content type: Research

    Acrylic acid (AA) is a widely used commodity chemical derived from non-renewable fossil fuel sources. Alternative microbial-based production methodologies are being developed with the aim of providing “green” ...

    Authors: Sarada S. Raghavan, Sharon Chee, Juntao Li, Jeremie Poschmann, Niranjan Nagarajan, Siau Jia Wei, Chandra S. Verma and Farid J. Ghadessy

    Citation: Microbial Cell Factories 2019 18:139

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  8. Content type: Research

    The development of sustainable technologies for plant cell wall degradation greatly depends on enzymes with hydrolytic activities against carbohydrates. The waste by-products of agricultural cereals are import...

    Authors: Tao Tu, Xiaoli Li, Kun Meng, Yingguo Bai, Yuan Wang, Zhenxing Wang, Bin Yao and Huiying Luo

    Citation: Microbial Cell Factories 2019 18:138

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  9. Content type: Research

    Microbial cell factories are widely used in the production of acidic products such as organic acids and amino acids. However, the metabolic activity of microbial cells and their production efficiency are sever...

    Authors: Zhengming Zhu, Jinhua Yang, Peishan Yang, Zhimeng Wu, Juan Zhang and Guocheng Du

    Citation: Microbial Cell Factories 2019 18:136

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  10. Content type: Research

    Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their su...

    Authors: Qianqian Zhuang and Qingsheng Qi

    Citation: Microbial Cell Factories 2019 18:135

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  11. Content type: Research

    The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow,...

    Authors: José M. Pastor, Nuno Borges, Juan P. Pagán, Sara Castaño-Cerezo, Laszlo N. Csonka, Bradley W. Goodner, Kathryn A. Reynolds, Luís G. Gonçalves, Montserrat Argandoña, Joaquín J. Nieto, Carmen Vargas, Vicente Bernal and Manuel Cánovas

    Citation: Microbial Cell Factories 2019 18:134

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  12. Content type: Research

    Heparosan is the unsulfated precursor of heparin and heparan sulfate and its synthesis is typically the first step in the production of bioengineered heparin. In addition to its utility as the starting materia...

    Authors: Asher Williams, Kamil S. Gedeon, Deepika Vaidyanathan, Yanlei Yu, Cynthia H. Collins, Jonathan S. Dordick, Robert J. Linhardt and Mattheos A. G. Koffas

    Citation: Microbial Cell Factories 2019 18:132

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  13. Content type: Research

    The overexpression and purification of membrane proteins is a bottleneck in biotechnology and structural biology. E. coli remains the host of choice for membrane protein production. To date, most of the efforts h...

    Authors: Kerstin Kanonenberg, Jorge Royes, Alexej Kedrov, Gereon Poschmann, Federica Angius, Audrey Solgadi, Olivia Spitz, Diana Kleinschrodt, Kai Stühler, Bruno Miroux and Lutz Schmitt

    Citation: Microbial Cell Factories 2019 18:131

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  14. Content type: Research

    Acetyl-CoA is an important metabolic intermediate and serves as an acetylation precursor for the biosynthesis of various value-added acetyl-chemicals. Acetyl-CoA can be produced from glucose, acetate, or fatty...

    Authors: Shasha Zhang, Wei Yang, Hao Chen, Bo Liu, Baixue Lin and Yong Tao

    Citation: Microbial Cell Factories 2019 18:130

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  15. Content type: Research

    Vitamin K2 (menaquinone, MK-n) is a lipid-soluble vitamin that functions as a carboxylase co-factor for maturation of proteins involved in many vital physiological processes in humans. Notably, long-chain vita...

    Authors: Yue Liu, Eric O. van Bennekom, Yu Zhang, Tjakko Abee and Eddy J. Smid

    Citation: Microbial Cell Factories 2019 18:129

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  16. Content type: Research

    Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate conversion ...

    Authors: Xian Zhang, Rumeng Han, Teng Bao, Xiaojing Zhao, Xiangfei Li, Manchi Zhu, Taowei Yang, Meijuan Xu, Minglong Shao, Youxi Zhao and Zhiming Rao

    Citation: Microbial Cell Factories 2019 18:128

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  17. Content type: Research

    Our laboratory has constructed a Bacillus licheniformis strain that secretes alkaline protease (AprE) with excellent enzymatic properties. B. licheniformis is generally regarded as safe and has a high industrial ...

    Authors: Cuixia Zhou, Huiying Zhou, Huitu Zhang and Fuping Lu

    Citation: Microbial Cell Factories 2019 18:127

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  18. Content type: Research

    Bacterial secretory proteins often require the formation of disulphide bonds outside the cell to acquire an active conformation. Thiol-disulphide oxidoreductases are enzymes that catalyse the formation of disu...

    Authors: Sonia Gullón, Silvia Marín and Rafael P. Mellado

    Citation: Microbial Cell Factories 2019 18:126

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  19. Content type: Research

    Threonine is an essential amino acid that is extensively used in livestock industry as feed supplement due to its pronounced effect in improving the growth performance of animals. Application of genetically en...

    Authors: Ye Heng Lim, Hooi Ling Foo, Teck Chwen Loh, Rosfarizan Mohamad, Raha Abdul Rahim and Zulkifli Idrus

    Citation: Microbial Cell Factories 2019 18:125

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  20. Content type: Research

    The microbial production of useful fuels and chemicals has been widely studied. In several cases, glucose is used as the raw material, and almost all microbes adopt the Embden–Meyerhof (EM) pathway to degrade ...

    Authors: Shuhei Noda, Yutaro Mori, Sachiko Oyama, Akihiko Kondo, Michihiro Araki and Tomokazu Shirai

    Citation: Microbial Cell Factories 2019 18:124

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  21. Content type: Research

    Myxococcus xanthus DK1622 is a model system for studying multicellular development, predation, cellular differentiation, and evolution. Furthermore, it is a rich source of novel secondary metabolites and is widel...

    Authors: Ying-jie Yang, Raghvendra Pratap Singh, Xin Lan, Cheng-sheng Zhang, Duo-hong Sheng and Yi-qiang Li

    Citation: Microbial Cell Factories 2019 18:123

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  22. Content type: Research

    Acetyl xylan esterase plays an important role in the complete enzymatic hydrolysis of lignocellulosic materials. It hydrolyzes the ester linkages of acetic acid in xylan and supports and enhances the activity ...

    Authors: Sachithra Amarin Hettiarachchi, Young-Kyung Kwon, Youngdeuk Lee, Eunyoung Jo, Tae-Yang Eom, Yoon-Hyeok Kang, Do-Hyung Kang, Mahanama De Zoysa, Svini Dileepa Marasinghe and Chulhong Oh

    Citation: Microbial Cell Factories 2019 18:122

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  23. Content type: Research

    Fungal perylenequinonoid (PQ) pigments from Shiraia fruiting body have been well known as excellent photosensitizers for medical and agricultural uses. The fruiting bodies are colonized by a diverse bacterial com...

    Authors: Yan Jun Ma, Li Ping Zheng and Jian Wen Wang

    Citation: Microbial Cell Factories 2019 18:121

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  24. Content type: Research

    Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing a...

    Authors: Wen Wang, Ping He, Dongdong Zhao, Lijun Ye, Longhai Dai, Xueli Zhang, Yuanxia Sun, Jing Zheng and Changhao Bi

    Citation: Microbial Cell Factories 2019 18:120

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  25. Content type: Research

    The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the va...

    Authors: Lukas Marsalek, Verena Puxbaum, Markus Buchetics, Diethard Mattanovich and Brigitte Gasser

    Citation: Microbial Cell Factories 2019 18:119

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  26. Content type: Research

    Enzymatic glycan synthesis has leapt forward in recent years and a number of glucuronosyltransferase (EC have been identified and prepared, which provides a guide to an efficient approach to prepare ...

    Authors: Dan-Hua Meng, Ran-Ran Du, Lu-Zhou Chen, Meng-Ting Li, Fei Liu, Jin Hou, Yi-Kang Shi, Feng-Shan Wang and Ju-Zheng Sheng

    Citation: Microbial Cell Factories 2019 18:118

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  27. Content type: Research

    Rhodosporidium toruloides is a promising host for the production of bioproducts from lignocellulosic biomass. A key prerequisite for efficient pathway engineering is the availability of robust genetic tools and r...

    Authors: Luísa Czamanski Nora, Maren Wehrs, Joonhoon Kim, Jan-Fang Cheng, Angela Tarver, Blake A. Simmons, Jon Magnuson, Miranda Harmon-Smith, Rafael Silva-Rocha, John M. Gladden, Aindrila Mukhopadhyay, Jeffrey M. Skerker and James Kirby

    Citation: Microbial Cell Factories 2019 18:117

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  28. Content type: Research

    Sucrose is an attractive industrial carbon source due to its abundance and the fact that it can be cheaply generated from sources such as sugarcane. However, only a few characterized Escherichia coli strains are ...

    Authors: Elsayed T. Mohamed, Hemanshu Mundhada, Jenny Landberg, Isaac Cann, Roderick I. Mackie, Alex Toftgaard Nielsen, Markus J. Herrgård and Adam M. Feist

    Citation: Microbial Cell Factories 2019 18:116

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  29. Content type: Research

    Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate syn...

    Authors: Yu Fei, Na Li, De-Huai Zhang and Jun-Wei Xu

    Citation: Microbial Cell Factories 2019 18:115

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  30. Content type: Research

    Actinoplanes sp. SE50/110 is a natural producer of acarbose. It has been extensively studied in the last decades, which has led to the comprehensive analysis of the whole genome, transcriptome and proteome. First...

    Authors: Lena Schaffert, Camilla März, Lisa Burkhardt, Julian Droste, David Brandt, Tobias Busche, Winfried Rosen, Susanne Schneiker-Bekel, Marcus Persicke, Alfred Pühler and Jörn Kalinowski

    Citation: Microbial Cell Factories 2019 18:114

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  31. Content type: Research

    Microalgae have been proposed as potential platform to produce lipid-derived products, such as biofuels. Knowledge on the intracellular carbon flow distribution may identify key metabolic processes during lipi...

    Authors: Xiaojie Ren, Jean-Sébastien Deschênes, Réjean Tremblay, Sabine Peres and Mario Jolicoeur

    Citation: Microbial Cell Factories 2019 18:113

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  32. Content type: Research

    Yaks living in the high-altitude hypoxic environment of Tibetan plateau (3600 m) have special gut microbes. However, it is still little research on yak probiotics until now. Therefore, the purpose of our study...

    Authors: Aoyun Li, Yaping Wang, Zhixing Li, Hammad Qamar, Khalid Mehmood, Lihong Zhang, Juanjuan Liu, Hui Zhang and Jiakui Li

    Citation: Microbial Cell Factories 2019 18:112

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  33. Content type: Research

    Bacillus subtilis is developed to be an attractive expression host to produce both secreted and cytoplasmic proteins owing to its prominent biological characteristics. Chromosomal integration is a stable expressi...

    Authors: Chaoyang Zhou, Bin Ye, Shan Cheng, Leizhen Zhao, Yuanxin Liu, Jiandong Jiang and Xin Yan

    Citation: Microbial Cell Factories 2019 18:111

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  34. Content type: Research

    Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopo...

    Authors: Hui Jin, Yunju Jeong, Sang-Ho Yoo, Tony V. Johnston, Seockmo Ku and Geun Eog Ji

    Citation: Microbial Cell Factories 2019 18:110

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  35. Content type: Research

    Metabolic models are indispensable in guiding cellular engineering and in advancing our understanding of systems biology. As not all enzymatic activities are fully known and/or annotated, metabolic models rema...

    Authors: Sara A. Amin, Elizabeth Chavez, Vladimir Porokhin, Nikhil U. Nair and Soha Hassoun

    Citation: Microbial Cell Factories 2019 18:109

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  36. Content type: Research

    In lactic acid bacteria (LAB), acid stress leads to decreases of cell vitality and fermentation yield. Glutamate decarboxylase (GAD) system is regarded as one of the essential acid-resistance mechanisms in LAB...

    Authors: Luchan Gong, Cong Ren and Yan Xu

    Citation: Microbial Cell Factories 2019 18:108

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  37. Content type: Research

    Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due ...

    Authors: Junshu Wang, Fapeng Liu, Tianyuan Su, Yizhao Chang, Qi Guo, Qian Wang, Quanfeng Liang and Qingsheng Qi

    Citation: Microbial Cell Factories 2019 18:107

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  38. Content type: Research

    Late-stage fermentation broth contains high concentrations of target chemicals. Additionally, it contains various cellular metabolites which have leaked from lysed cells, which would exert multifactorial stres...

    Authors: Xiaowei Wang, Qinggang Li, Cunmin Sun, Zhen Cai, Xiaomei Zheng, Xuan Guo, Xiaomeng Ni, Wenjuan Zhou, Yanmei Guo, Ping Zheng, Ning Chen, Jibin Sun, Yin Li and Yanhe Ma

    Citation: Microbial Cell Factories 2019 18:106

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  39. Content type: RESEARCH

    Geraniol, an acyclic monoterpene alcohol, is found as a primary constituent in the essential oils of plants such as geranium, lemongrass and rose. The floral-like scent of geraniol has made it a popular consti...

    Authors: Micaela G. Chacón, Alice Marriott, Emanuele G. Kendrick, Matthew Q. Styles and David J. Leak

    Citation: Microbial Cell Factories 2019 18:105

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  40. Content type: Research

    Methylocystis parvus is a type II methanotroph characterized by its high specific methane degradation rate (compared to other methanotrophs of the same family) and its ability to accumulate up to 50% of its bioma...

    Authors: Sergio Bordel, Antonia Rojas and Raúl Muñoz

    Citation: Microbial Cell Factories 2019 18:104

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  41. Content type: Research

    Pseudorabies caused by pseudorabies virus (PRV) mainly infects the swine and seriously threatens the biosafety of the other animals, including humans. Since 2011, the outbreaks of PRV mutants have caused enorm...

    Authors: Jialu Wang, Yongheng Wang, En Zhang, Mengyun Zhou, Jian Lin and Qian Yang

    Citation: Microbial Cell Factories 2019 18:103

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  42. Content type: Research

    The global prevalence of traditional Chinese medicine stimulates the prosperous development of herb medicines, but the annual generation of massive herb residues becomes big issues about environmental pollutio...

    Authors: Wenping Kong, Chengshuang Huang, Jie Shi, Yu Li, Xinxin Jiang, Quwen Duan, Yong Huang, Yanwen Duan and Xiangcheng Zhu

    Citation: Microbial Cell Factories 2019 18:102

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  43. Content type: Research

    Many fermented foods and beverages are produced through the action of complex microbial communities. Synthetic biology approaches offer the ability to genetically engineer these communities to improve the prop...

    Authors: Rachatida Det-udom, Charlie Gilbert, Long Liu, Cheunjit Prakitchaiwattana, Tom Ellis and Rodrigo Ledesma-Amaro

    Citation: Microbial Cell Factories 2019 18:101

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  44. Content type: Research

    Bacillus subtilis spores have been commonly used for the surface display of various food-related or human antigens or enzymes. For successful display, the target protein needs to be fused with an anchor protein. ...

    Authors: Hongling Liu, Shaojie Yang, Xihui Wang and Tengfei Wang

    Citation: Microbial Cell Factories 2019 18:100

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  45. Content type: Research

    The oleaginous yeast Yarrowia lipolytica is an organism of choice for the tailored production of various compounds such as biofuels or biopolymers. When properly engineered, it is capable of producing medium-chai...

    Authors: Coraline Rigouin, Sophie Lajus, Connie Ocando, Vinciane Borsenberger, Jean Marc Nicaud, Alain Marty, Luc Avérous and Florence Bordes

    Citation: Microbial Cell Factories 2019 18:99

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  46. Content type: Research

    Farnesol is an acyclic sesquiterpene alcohol present in the essential oils of various plants in nature. It has been reported to be valuable in medical applications, such as alleviation of allergic asthma, glio...

    Authors: Xueduan Chen, Xianzhang Jiang, Man Xu, Mingliang Zhang, Runye Huang, Jianzhong Huang and Feng Qi

    Citation: Microbial Cell Factories 2019 18:98

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  47. Content type: Research

    Transglycosylation represents one of the most promising approaches for obtaining novel glycosides, and plant phenols and polyphenols are emerging as one of the best targets for creating new molecules with enha...

    Authors: Juan Antonio Méndez-Líter, Isabel Tundidor, Manuel Nieto-Domínguez, Beatriz Fernández de Toro, Andrés González Santana, Laura Isabel de Eugenio, Alicia Prieto, Juan Luis Asensio, Francisco Javier Cañada, Cristina Sánchez and María Jesús Martínez

    Citation: Microbial Cell Factories 2019 18:97

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  48. Content type: Research

    Promoter evolution by synthetic promoter library (SPL) is a powerful approach to development of functional synthetic promoters to synthetic biology. However, it requires much tedious and time-consuming screeni...

    Authors: Laichuang Han, Wenjing Cui, Feiya Suo, Shengnan Miao, Wenliang Hao, Qiaoqing Chen, Junling Guo, Zhongmei Liu, Li Zhou and Zhemin Zhou

    Citation: Microbial Cell Factories 2019 18:96

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