Articles

Expression of the human molecular chaperone domain Bri2 BRICHOS on a gram per liter scale with an E. coli fed-batch culture

The human Bri2 BRICHOS domain inhibits amyloid formation and toxicity and could be used as a therapeutic agent against amyloid diseases. For translation into clinical use, large quantities of correctly folded ...

Authors: Benjamin Schmuck, Gefei Chen, Josef Pelcman, Nina Kronqvist, Anna Rising and Jan Johansson

Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum

The intracellular ATP level is an indicator of cellular energy state and plays a critical role in regulating cellular metabolism. Depletion of intracellular ATP in (facultative) aerobes can enhance glycolysis,...

Authors: Zongjie Dai, Yan Zhu, Hongjun Dong, Chunhua Zhao, Yanping Zhang and Yin Li

Fine-tuning the expression of pathway gene in yeast using a regulatory library formed by fusing a synthetic minimal promoter with different Kozak variants

Tailoring gene expression to balance metabolic fluxes is critical for the overproduction of metabolites in yeast hosts, and its implementation requires coordinated regulation at both transcriptional and transl...

Authors: Liping Xu, Pingping Liu, Zhubo Dai, Feiyu Fan and Xueli Zhang

Engineering of Saccharomyces cerevisiae for the accumulation of high amounts of triacylglycerol

Fatty acid-based substances play an important role in many products, from food supplements to pharmaceutical products and biofuels. The production of fatty acids, mainly in their esterified form as triacylglyc...

Authors: Simon Arhar, Gabriela Gogg-Fassolter, Mojca Ogrizović, Klavdija Pačnik, Katharina Schwaiger, Mia Žganjar, Uroš Petrovič and Klaus Natter

A cell engineering approach to enzyme-based fed-batch fermentation

A fundamental problem associated with E. coli fermentations is the difficulty in achieving high cell densities in batch cultures, attributed in large part to the production and accumulation of acetate through a p...

Authors: Michael Sibley and John M. Ward

Production of trans-cinnamic acid by whole-cell bioconversion from l-phenylalanine in engineered Corynebacterium glutamicum

trans-cinnamic acid (t-CA) is a phenylpropanoid with a broad spectrum of biological activities including antioxidant and antibacterial activities, and it also has high potential in food and cosmetic applications...

Authors: Jaewoo Son, Jun Hong Jang, In Hyeok Choi, Chang Gyu Lim, Eun Jung Jeon, Hyun Bae Bang and Ki Jun Jeong

The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production

Adenosine 5′-triphosphate (ATP) plays both a central role as an intracellular energy source, and a crucial extracellular signaling role in diverse physiological processes of animals and plants. However, there ...

Authors: Xin Ping Li, Lu Lu Zhou, Yan Hua Guo and Jian Wen Wang

Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris

The antimicrobial peptide LL37 is produced by white blood cells (mainly neutrophils) and various epithelial cells, and has the outstanding advantages of participating in immune regulation, causing chemotaxis o...

Authors: Na Zhan, Licong Zhang, Hong Yang, Yalan Zheng, Xinke Wei, Jiajun Wang and Anshan Shan

Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

Vanillin is one of the important phenolic inhibitors in Saccharomyces cerevisiae for bioconversion of lignocellulosic materials and has been reported to inhibit the translation process in cells. In our previous s...

Authors: Wenyan Cao, Weiquan Zhao, Bolun Yang, Xinning Wang, Yu Shen, Tiandi Wei, Wensheng Qin, Zailu Li and Xiaoming Bao

Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa

Acetoin utilization protein (acuC) is a type I histone deacetylase which is highly conserved in bacteria. The acuC gene is related to the acetylation/deacetylation posttranslational modification (PTM) system in S...

Authors: Zhudong Liu, Jie Xiao, Jianli Tang, Yang Liu, Ling Shuai, Li Cao, Ziyuan Xia, Xuezhi Ding, Jie Rang and Liqiu Xia

Correction to: Gold standard for nutrition: a review of human milk oligosaccharide and its effects on infant gut microbiota

Authors: Shunhao Zhang, Tianle Li, Jing Xie, Demao Zhang, Caixia Pi, Lingyun Zhou and Wenbin Yang

Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases

Biological nitrogen fixation is catalyzed by Mo-, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively. NifB is the key protein in synthesis of the cofactors of all nitrogenases. Most d...

Authors: Qin Li, Haowei Zhang, Liqun Zhang and Sanfeng Chen

Co-culturing experiments reveal the uptake of myo-inositol phosphate synthase (EC 5.5.1.4) in an inositol auxotroph of Saccharomyces cerevisiae

Myo-Inositol Phosphate Synthase (MIP) catalyzes the conversion of glucose 6- phosphate into inositol phosphate, an essential nutrient and cell signaling molecule. Data obtained, first in bovine brain and later...

Authors: Erika Steele, Hana D. Alebous, Macy Vickers, Mary E. Harris and Margaret D. Johnson

Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress

In China, during the cultivation process of Pleurotus ostreatus, the yield and quality of fruiting bodies are easily affected by high temperatures in summer. Nitric oxide (NO) plays an important regulatory role i...

Authors: Ludan Hou, Mengran Zhao, Chenyang Huang, Qi He, Lijiao Zhang and Jinxia Zhang

Cellulose induced protein 1 (Cip1) from Trichoderma reesei enhances the enzymatic hydrolysis of pretreated lignocellulose

Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 (Cip1) is one of the most abundant proteins in extracellular proteins of T. reesei. Reported...

Authors: Hexue Jia, Wan Sun, Xuezhi Li and Jian Zhao

Impact of organic carbon acquisition on growth and functional biomolecule production in diatoms

Diatoms are unicellular photosynthetic protists which constitute one of the most successful microalgae contributing enormously to global primary productivity and nutrient cycles in marine and freshwater habita...

Authors: Thomas Kiran Marella, Raya Bhattacharjya and Archana Tiwari

Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

Monoclonal antibodies, antibody fragments and fusion proteins derived thereof have revolutionized the practice of medicine. Major challenges faced by the biopharmaceutical industry are however high production...

Authors: Yanyan Wang, Xiaowei Li, Xin Chen, Jens Nielsen, Dina Petranovic and Verena Siewers

Biodegradation of flonicamid by Ensifer adhaerens CGMCC 6315 and enzymatic characterization of the nitrile hydratases involved

Flonicamid (N-cyanomethyl-4-trifluoromethylnicotinamide, FLO) is a new type of pyridinamide insecticide that regulates insect growth. Because of its wide application in agricultural production and high solubility...

Authors: Yun-Xiu Zhao, Li Wang, Ke-Xin Chen, Neng-Dang Jiang, Shi-Lei Sun, Feng Ge and Yi-Jun Dai

Systematic identification of a panel of strong promoter regions from Listeria monocytogenes for fine-tuning gene expression

Attenuated Listeria monocytogenes (Lm) has been widely used as a vaccine vector in the prevention and treatment of pathogen infection and tumor diseases. In addition, previous studies have proved that the atten...

Authors: Qianyu Ji, Junfei Ma, Shuying Wang and Qing Liu

Augmented peroxisomal ROS buffering capacity renders oxidative and thermal stress cross-tolerance in yeast

Thermotolerant yeast has outstanding potential in industrial applications. Komagataella phaffii (Pichia pastoris) is a common cell factory for industrial production of heterologous proteins.

Authors: Nai-Xin Lin, Rui-Zhen He, Yan Xu and Xiao-Wei Yu

Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number

Synechocystis sp. PCC 6803 provides a well-established reference point to cyanobacterial metabolic engineering as part of basic photosynthesis research, as well as in the development of next-generation biotechnol...

Authors: Csaba Nagy, Kati Thiel, Edita Mulaku, Henna Mustila, Paula Tamagnini, Eva-Mari Aro, Catarina C. Pacheco and Pauli Kallio

Characterization of glycoside hydrolase family 11 xylanase from Streptomyces sp. strain J103; its synergetic effect with acetyl xylan esterase and enhancement of enzymatic hydrolysis of lignocellulosic biomass

Xylanase-containing enzyme cocktails are used on an industrial scale to convert xylan into value-added products, as they hydrolyse the β-1,4-glycosidic linkages between xylopyranosyl residues. In the present s...

Authors: Svini Dileepa Marasinghe, Eunyoung Jo, Sachithra Amarin Hettiarachchi, Youngdeuk Lee, Tae-Yang Eom, Yehui Gang, Yoon-Hyeok Kang and Chulhong Oh

Artificial complementary chromatic acclimation gene expression system in Escherichia coli

The development of multiple gene expression systems, especially those based on the physical signals, such as multiple color light irradiations, is challenging. Complementary chromatic acclimation (CCA), a phot...

Authors: Dwi Ariyanti, Kazunori Ikebukuro and Koji Sode

Early response of methanogenic archaea to H₂ as evaluated by metagenomics and metatranscriptomics

The molecular machinery of the complex microbiological cell factory of biomethane production is not fully understood. One of the process control elements is the regulatory role of hydrogen (H₂). Reduction of carb...

Authors: Balázs Kakuk, Roland Wirth, Gergely Maróti, Márk Szuhaj, Gábor Rakhely, Krisztián Laczi, Kornél L. Kovács and Zoltán Bagi

Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls

The fungal cell wall is an essential and robust external structure that protects the cell from the environment. It is mainly composed of polysaccharides with different functions, some of which are necessary fo...

Authors: Vanessa S. D. Carvalho, Laura Gómez-Delgado, M. Ángeles Curto, M. Belén Moreno, Pilar Pérez, Juan Carlos Ribas and Juan Carlos G. Cortés

Integration of enzyme constraints in a genome-scale metabolic model of Aspergillus niger improves phenotype predictions

Genome-scale metabolic model (GSMM) is a powerful tool for the study of cellular metabolic characteristics. With the development of multi-omics measurement techniques in recent years, new methods that integrat...

Authors: Jingru Zhou, Yingping Zhuang and Jianye Xia

Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

The manufacture of recombinant therapeutics is a fastest-developing section of therapeutic pharmaceuticals and presently plays a significant role in disease management. Yeasts are established eukaryotic host f...

Authors: Aravind Madhavan, K. B. Arun, Raveendran Sindhu, Jayaram Krishnamoorthy, R. Reshmy, Ranjna Sirohi, Arivalagan Pugazhendi, Mukesh Kumar Awasthi, George Szakacs and Parameswaran Binod

Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production

Klebsiella pneumoniae is a bacterium that can be used as producer for numerous chemicals. Glycerol can be catabolised by K. pneumoniae and dihydroxyacetone is an intermediate of this catabolism pathway. Here dihy...

Authors: Shaoqi Sun, Yike Wang, Lin Shu, Xiyang Lu, Qinghui Wang, Chenguang Zhu, Jiping Shi, Gary J. Lye, Frank Baganz and Jian Hao

Enterococcus faecium PNC01 isolated from the intestinal mucosa of chicken as an alternative for antibiotics to reduce feed conversion rate in broiler chickens

The development and utilization of probiotics had many environmental benefits for replacing antibiotics in animal production. Bacteria in the intestinal mucosa have better adhesion to the host intestinal epith...

Authors: Yang He, Xuan Liu, Yuanyang Dong, Jiaqi Lei, Koichi Ito and Bingkun Zhang

Metabolic engineering of Escherichia coli for de novo production of 3-phenylpropanol via retrobiosynthesis approach

3-Phenylpropanol with a pleasant odor is widely used in foods, beverages and cosmetics as a fragrance ingredient. It also acts as the precursor and reactant in pharmaceutical and chemical industries. Currently...

Authors: Zhenning Liu, Xue Zhang, Dengwei Lei, Bin Qiao and Guang-Rong Zhao

A critical review on various feedstocks as sustainable substrates for biosurfactants production: a way towards cleaner production

The quest for a chemical surfactant substitute has been fuelled by increased environmental awareness. The benefits that biosurfactants present like biodegradability, and biocompatibility over their chemical an...

Authors: Swayansu Sabyasachi Mohanty, Yamini Koul, Sunita Varjani, Ashok Pandey, Huu Hao Ngo, Jo-Shu Chang, Jonathan W. C. Wong and Xuan-Thanh Bui

Universal capability of 3-ketosteroid Δ¹-dehydrogenases to catalyze Δ¹-dehydrogenation of C17-substituted steroids

3-Ketosteroid Δ¹-dehydrogenases (KSTDs) are the enzymes involved in microbial cholesterol degradation and modification of steroids. They catalyze dehydrogenation between C1 and C2 atoms in ring A of the polycycli...

Authors: Patrycja Wójcik, Michał Glanowski, Agnieszka M. Wojtkiewicz, Ali Rohman and Maciej Szaleniec

Genetic tools for the development of recombinant lactic acid bacteria

Lactic acid bacteria (LAB) are a phylogenetically diverse group with the ability to convert soluble carbohydrates into lactic acid. Many LAB have a long history of safe use in fermented foods and are recognize...

Authors: Jiapeng Wu, Yongping Xin, Jian Kong and Tingting Guo

Enzyme-catalyzed biodegradation of penicillin fermentation residues by β-lactamase OtLac from Ochrobactrum tritici

Biodegradation of antibiotics is a promising method for the large-scale removal of antibiotic residues in the environment. However, the enzyme that is involved in the biodegradation process is the key informat...

Authors: Peng Wang, Chen Shen, Qinqin Cong, Kaili Xu and Jialin Lu

Refactoring of a synthetic raspberry ketone pathway with EcoFlex

 A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to ...

Authors: Simon J. Moore, Yonek B. Hleba, Sarah Bischoff, David Bell, Karen M. Polizzi and Paul S. Freemont

Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives

Interleukin-15 (IL-15) is a critical cytokine for the development, proliferation, and function of natural killer (NK) cells, NKT cells, and CD8⁺ memory T cells and has become one of the most promising protein mol...

Authors: Huan Xu, Mingyang Shi, Changsheng Shao, Hao Li, Jing Wu, Yin Yu, Fang Fang, Yugang Guo and Weihua Xiao

In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain

The current shift from a fossil-resource based economy to a more sustainable, bio-based economy requires development of alternative production routes based on utilization of biomass for the many chemicals that...

Authors: Thomas Nicolaï, Quinten Deparis, María R. Foulquié-Moreno and Johan M. Thevelein

Site-directed mutagenesis of the quorum-sensing transcriptional regulator SinR affects the biosynthesis of menaquinone in Bacillus subtilis

Menaquinone (MK-7) is a highly valuable vitamin K₂ produced by Bacillus subtilis. Common static metabolic engineering approaches for promoting the production of MK-7 have been studied previously. However, these a...

Authors: Jing Wu, Wei Li, Shi-guang Zhao, Sen-he Qian, Zhou Wang, Meng-jie Zhou, Wen-song Hu, Jian Wang, Liu-xiu Hu, Yan Liu and Zheng-lian Xue

Emerging prospects of macro- and microalgae as prebiotic

Macro- and microalgae-based foods are becoming popular due to their high nutritious value. The algal biomass is enriched with polysaccharides, protein, polyunsaturated fatty acids, carotenoids, vitamins and mi...

Authors: Anil Kumar Patel, Reeta Rani Singhania, Mukesh Kumar Awasthi, Sunita Varjani, Shashi Kant Bhatia, Mei-Ling Tsai, Shu-Ling Hsieh, Chiu-Wen Chen and Cheng-Di Dong

Superior production of heavy pamamycin derivatives using a bkdR deletion mutant of Streptomyces albus J1074/R2

Pamamycins are macrodiolides of polyketide origin which form a family of differently large homologues with molecular weights between 579 and 663. They offer promising biological activity against pathogenic fun...

Authors: Lars Gläser, Martin Kuhl, Julian Stegmüller, Christian Rückert, Maksym Myronovskyi, Jörn Kalinowski, Andriy Luzhetskyy and Christoph Wittmann

The cssR gene of Corynebacterium glutamicum plays a negative regulatory role in stress responses

CssR, the product of the Corynebacterium glutamicum ncgl1578 gene cotranscribed with ncgl1579, is a TetR (tetracycline regulator) family repressor. Although many TetR-type regulators in C. glutamicum have been ex...

Authors: Yang Liu, Wenzhi Yang, Tao Su, Chengchuan Che, Guizhi Li, Can Chen and Meiru Si

Genome-based selection and application of food-grade microbes for chickpea milk fermentation towards increased l-lysine content, elimination of indigestible sugars, and improved flavour

Plant-based milk alternatives are more popular than ever, and chickpea-based milks are among the most commercially relevant products. Unfortunately, limited nutritional value because of low levels of the essen...

Authors: Muzi Tangyu, Michel Fritz, Rosa Aragao-Börner, Lijuan Ye, Biljana Bogicevic, Christoph J. Bolten and Christoph Wittmann

Gold standard for nutrition: a review of human milk oligosaccharide and its effects on infant gut microbiota

Human milk is the gold standard for nutrition of infant growth, whose nutritional value is mainly attributed to human milk oligosaccharides (HMOs). HMOs, the third most abundant component of human milk after l...

Authors: Shunhao Zhang, Tianle Li, Jing Xie, Demao Zhang, Caixia Pi, Lingyun Zhou and Wenbin Yang

Unlocking the potential of insect and ruminant host symbionts for recycling of lignocellulosic carbon with a biorefinery approach: a review

Uprising fossil fuel depletion and deterioration of ecological reserves supply have led to the search for alternative renewable and sustainable energy sources and chemicals. Although first generation biorefine...

Authors: Gunasekaran Rajeswari, Samuel Jacob, Anuj Kumar Chandel and Vinod Kumar

Adaptive laboratory evolution of β-caryophyllene producing Saccharomyces cerevisiae

β-Caryophyllene is a plant terpenoid with therapeutic and biofuel properties. Production of terpenoids through microbial cells is a potentially sustainable alternative for production. Adaptive laboratory evolu...

Authors: Avinash Godara and Katy C. Kao

Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor

Commercial xylose purification produces xylose mother liquor (XML) as a major byproduct, which has become an inexpensive and abundant carbon source. A portion of this XML has been used to produce low-value-add...

Authors: Lihua Zhang, Zhen Chen, Junhua Wang, Wei Shen, Qi Li and Xianzhong Chen

Development of an automated platform for the optimal production of glycoconjugate vaccines expressed in Escherichia coli

Protein Glycan Coupling Technology (PGCT) uses purposely modified bacterial cells to produce recombinant glycoconjugate vaccines. This vaccine platform holds great potential in this context, namely due to its ...

Authors: Jasmin J. Samaras, Marta Mauri, Emily J. Kay, Brendan W. Wren and Martina Micheletti

Anaerobic biosynthesis of rhamnolipids by Pseudomonas aeruginosa: performance, mechanism and its application potential for enhanced oil recovery

Pseudomonas aeruginosa, the rhamnolipids-producer, is one of dominant bacteria in oil reservoirs. Although P. aeruginosa strains are facultative bacteria, the anaerobic biosynthesis mechanism of rhamnolipids is u...

Authors: Feng Zhao, Qingzhi Wang, Ying Zhang and Liying Lei

Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis

As treatment of Staphylococcus aureus (S. aureus) osteomyelitis is often hindered by the development of antibiotic tolerance, novel antibacterial therapeutics are required. Here we found that the cell-free supern...

Authors: Fan Zhang, Bowei Wang, Shiluan Liu, Yuhui Chen, Yihuang Lin, Zixian Liu, Xianrong Zhang and Bin Yu

Biosynthesis of pinene in purple non-sulfur photosynthetic bacteria

Pinene is a monoterpene, that is used in the manufacture of fragrances, insecticide, fine chemicals, and renewable fuels. Production of pinene by metabolic-engineered microorganisms is a sustainable method. Pu...

Authors: Xiaomin Wu, Guang Ma, Chuanyang Liu, Xin-yuan Qiu, Lu Min, Jingyu Kuang and Lingyun Zhu