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  1. Content type: RESEARCH

    The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs). Howeve...

    Authors: Animesh Sarker, Abhishek Singh Rathore and Rinkoo Devi Gupta

    Citation: Microbial Cell Factories 2019 18:5

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  2. Content type: RESEARCH

    The shortage of food based feedstocks has been one of the stumbling blocks in industrial biomanufacturing. The acetone bioproduction from the traditional acetone–butanol–ethanol fermentation is limited by the ...

    Authors: Hao Yang, Bing Huang, Ningyu Lai, Yang Gu, Zhimin Li, Qin Ye and Hui Wu

    Citation: Microbial Cell Factories 2019 18:6

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  3. Content type: RESEARCH

    As an essential platform chemical mostly used for rubber synthesis, isoprene is produced in industry through chemical methods, derived from petroleum. As an alternative, bio-production of isoprene has attracte...

    Authors: Meijie Li, Hailin Chen, Changqing Liu, Jing Guo, Xin Xu, Haibo Zhang, Rui Nian and Mo Xian

    Citation: Microbial Cell Factories 2019 18:4

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  4. Content type: RESEARCH

    Genome-scale metabolic models (GEMs) allow predicting metabolic phenotypes from limited data on uptake and secretion fluxes by defining the space of all the feasible solutions and excluding physio-chemically a...

    Authors: Ilaria Massaiu, Lorenzo Pasotti, Nikolaus Sonnenschein, Erlinda Rama, Matteo Cavaletti, Paolo Magni, Cinzia Calvio and Markus J. Herrgård

    Citation: Microbial Cell Factories 2019 18:3

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  5. Content type: RESEARCH

    Glucosamine-6-phosphate N-acetyltransferase (GNA1) is the key enzyme that causes overproduction of N-acetylglucosamine in Bacillus subtilis. Previously, we increased GlcNAc production by promoting the expression ...

    Authors: Wenlong Ma, Yanfeng Liu, Xueqin Lv, Jianghua Li, Guocheng Du and Long Liu

    Citation: Microbial Cell Factories 2019 18:1

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  6. Content type: Review

    Biosurfactants are surface-active agents produced by microorganisms that have higher efficiency and stability, lower toxicity and higher biocompatibility and biodegradability than chemical surfactants. Despite...

    Authors: Hélvia W. C. Araújo, Rosileide F. S. Andrade, Dayana Montero-Rodríguez, Daylin Rubio-Ribeaux, Carlos A. Alves da Silva and Galba M. Campos-Takaki

    Citation: Microbial Cell Factories 2019 18:2

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  7. Content type: RESEARCH

    The catabolite control protein A (CcpA) is a master regulator of many important cellular processes in Gram-positive bacteria. In Lactobacillus plantarum, CcpA directly or indirectly controls the transcription of ...

    Authors: Yanqing Lu, Sichao Song, Huaixiang Tian, Haiyan Yu, Jianxin Zhao and Chen Chen

    Citation: Microbial Cell Factories 2018 17:201

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  8. Content type: RESEARCH

    Corynebacterium glutamicum is a well-known producer of various l-amino acids in industry. During the fermenting process, C. glutamicum unavoidably encounters oxidative stress due to a specific reactive oxygen spe...

    Authors: Meiru Si, Tao Su, Can Chen, Jinfeng Liu, Zhijin Gong, Chengchuan Che, GuiZhi Li and Ge Yang

    Citation: Microbial Cell Factories 2018 17:200

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  9. Content type: RESEARCH

    The secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activit...

    Authors: Luminita Gabriela Horga, Samantha Halliwell, Tania Selas Castiñeiras, Chris Wyre, Cristina F. R. O. Matos, Daniela S. Yovcheva, Ross Kent, Rosa Morra, Steven G. Williams, Daniel C. Smith and Neil Dixon

    Citation: Microbial Cell Factories 2018 17:199

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  10. Content type: RESEARCH

    The Gram-positive Streptomyces lividans TK24 is an attractive host for heterologous protein production because of its high capability to secrete proteins—which favors correct folding and facilitates downstream pr...

    Authors: Wouter Daniels, Jeroen Bouvin, Tobias Busche, Christian Rückert, Kenneth Simoens, Spyridoula Karamanou, Lieve Van Mellaert, Ólafur H. Friðjónsson, Bart Nicolai, Anastassios Economou, Jörn Kalinowski, Jozef Anné and Kristel Bernaerts

    Citation: Microbial Cell Factories 2018 17:198

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  11. Content type: RESEARCH

    Butadiene is a platform chemical used as an industrial feedstock for the manufacture of automobile tires, synthetic resins, latex and engineering plastics. Currently, butadiene is predominantly synthesized as ...

    Authors: Jing Yang, Chang-Tai Zhang, Xiao-Jie Yuan, Min Zhang, Xu-Hua Mo, Ling-Ling Tan, Li-Ping Zhu, Wen-Jing Chen, Ming-Dong Yao, Bo Hu and Song Yang

    Citation: Microbial Cell Factories 2018 17:194

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  12. Content type: RESEARCH

    Scaling up of bioprocesses represents a crucial step in the industrial production of biologicals. However, our knowledge about the impact of scale-up on the organism’s physiology and function is still incomple...

    Authors: Juan C. Aon, Ricardo C. Tecson and Vakhtang Loladze

    Citation: Microbial Cell Factories 2018 17:195

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  13. Content type: Review

    Gram-negative bacteria are attractive hosts for recombinant protein production because they are fast growing, easy to manipulate, and genetically stable in large cultures. However, the utility of these microbe...

    Authors: Lisa Ann Burdette, Samuel Alexander Leach, Han Teng Wong and Danielle Tullman-Ercek

    Citation: Microbial Cell Factories 2018 17:196

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  14. Content type: RESEARCH

    Although interactions between microorganisms involved in biogas production are largely uncharted, it is commonly accepted that methanogenic Archaea are essential for the process. Methanogens thrive in various env...

    Authors: Adam Pyzik, Martyna Ciezkowska, Pawel S. Krawczyk, Adam Sobczak, Lukasz Drewniak, Andrzej Dziembowski and Leszek Lipinski

    Citation: Microbial Cell Factories 2018 17:197

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  15. Content type: RESEARCH

    Beyond pathway engineering, the metabolic state of the production host is critical in maintaining the efficiency of cellular production. The biotechnologically important yeast Saccharomyces cerevisiae adjusts its...

    Authors: Maren Wehrs, Jan-Philip Prahl, Jadie Moon, Yuchen Li, Deepti Tanjore, Jay D. Keasling, Todd Pray and Aindrila Mukhopadhyay

    Citation: Microbial Cell Factories 2018 17:193

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  16. Content type: RESEARCH

    Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of des...

    Authors: Saiful F. Haq, Anirudh P. Shanbhag, Subbulakshmi Karthikeyan, Imran Hassan, Kannan Thanukrishnan, Abhishek Ashok, Sunilkumar Sukumaran, S. Ramaswamy, Nagakumar Bharatham, Santanu Datta, Shalaka Samant and Nainesh Katagihallimath

    Citation: Microbial Cell Factories 2018 17:192

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  17. Content type: RESEARCH

    Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves ...

    Authors: Anna Bonhoure, Auguste Demenge, Camille Kostmann, Leticia San José, Eva De la Cal, Pilar Armisen, Yves Nominé and Gilles Travé

    Citation: Microbial Cell Factories 2018 17:191

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  18. Content type: RESEARCH

    Efficient microbial production of chemicals is often hindered by the cytotoxicity of the products or by the pathogenicity of the host strains. Hence 2,3-butanediol, an important drop-in chemical, is an interes...

    Authors: Anna Maria Erian, Martin Gibisch and Stefan Pflügl

    Citation: Microbial Cell Factories 2018 17:190

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  19. Content type: RESEARCH

    In Streptomyces, understanding the switch from primary to secondary metabolism is important for maximizing the production of secondary metabolites such as antibiotics, as well as for optimizing recombinant glycop...

    Authors: Ramsés A. Gamboa-Suasnavart, Norma A. Valdez-Cruz, Gerardo Gaytan-Ortega, Greta I. Reynoso-Cereceda, Daniel Cabrera-Santos, Lorena López-Griego, Wolf Klöckner, Jochen Büchs and Mauricio A. Trujillo-Roldán

    Citation: Microbial Cell Factories 2018 17:189

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  20. Content type: RESEARCH

    d-Psicose 3-epimerase (DPEase) catalyzes the isomerization of d-fructose to the rare sugar d-psicose, which may help prevent obesity, reduce blood sugar and blood fat, and inhibit intra-abdominal fat accumulation...

    Authors: Lingqia Su, Fan Sun, Zhanzhi Liu, Kang Zhang and Jing Wu

    Citation: Microbial Cell Factories 2018 17:188

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  21. Content type: RESEARCH

    We previously engineered Bacillus subtilis to express an antigen of interest fused to TasA in a biofilm. B. subtilis has several properties such as sporulation, biofilm formation and probiotic ability that were u...

    Authors: Cédric M. Vogt, Monika Hilbe, Mathias Ackermann, Claudio Aguilar and Catherine Eichwald

    Citation: Microbial Cell Factories 2018 17:187

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  22. Content type: RESEARCH

    The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical appli...

    Authors: Alexander Pekarsky, Lukas Veiter, Vignesh Rajamanickam, Christoph Herwig, Clemens Grünwald-Gruber, Friedrich Altmann and Oliver Spadiut

    Citation: Microbial Cell Factories 2018 17:183

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  23. Content type: RESEARCH

    The viral or host systems for a gene expression assume repeatability of the process and high quality of the protein product. Since level and fidelity of transcription primarily determines the overall efficienc...

    Authors: Dawid Koscielniak, Ewa Wons, Karolina Wilkowska and Marian Sektas

    Citation: Microbial Cell Factories 2018 17:184

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  24. Content type: RESEARCH

    Promoters are commonly used to regulate the expression of specific target genes or operons. Although a series of promoters have been developed in Corynebacterium glutamicum, more precise and unique expression pat...

    Authors: Yuechao Ma, Yi Cui, Lihong Du, Xiaoqian Liu, Xixian Xie and Ning Chen

    Citation: Microbial Cell Factories 2018 17:185

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  25. Content type: RESEARCH

    The chloroplast of eukaryotic microalgae such as Chlamydomonas reinhardtii is a potential platform for metabolic engineering and the production of recombinant proteins. In industrial biotechnology, inducible expr...

    Authors: Rosanna Young and Saul Purton

    Citation: Microbial Cell Factories 2018 17:186

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  26. Content type: RESEARCH

    Vegetable ‘mandi’ (road-side vegetable market) waste was converted to a suitable fermentation medium for cultivation of oleaginous yeast Rhodosporidium toruloides by steaming under pressure. This cultivation medi...

    Authors: Gunjan Singh, Sweta Sinha, K. K. Bandyopadhyay, Mark Lawrence and Debarati Paul

    Citation: Microbial Cell Factories 2018 17:182

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  27. Content type: RESEARCH

    Forskolin is a high-value diterpenoid produced exclusively by the Lamiaceae plant Coleus forskohlii. Today forskolin is used pharmaceutically for its adenyl-cyclase activating properties. The limited availability...

    Authors: Victor Forman, Niels Bjerg-Jensen, Jane D. Dyekjær, Birger Lindberg Møller and Irini Pateraki

    Citation: Microbial Cell Factories 2018 17:181

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  28. Content type: RESEARCH

    The glutamate decarboxylase (GAD) system of Lactobacillus brevis involves two isoforms of GAD, GadA and GadB, which catalyze the conversion of L-glutamate to γ-aminobutyric acid (GABA) in a proton-consuming react...

    Authors: Changjiang Lyu, Weirui Zhao, Chunlong Peng, Sheng Hu, Hui Fang, Yujiao Hua, Shanjing Yao, Jun Huang and Lehe Mei

    Citation: Microbial Cell Factories 2018 17:180

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  29. Content type: RESEARCH

    N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing. We have previously demonstrated t.....

    Authors: Wah Seng See-Too, Peter Convey, David A. Pearce and Kok-Gan Chan

    Citation: Microbial Cell Factories 2018 17:179

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  30. Content type: RESEARCH

    Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal de...

    Authors: Cristiana Faria, Nuno Borges, Isabel Rocha and Helena Santos

    Citation: Microbial Cell Factories 2018 17:178

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  31. Content type: RESEARCH

    Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) selectively eliminates tumor cells. However, the short biological half-life of this molecule limits its potential use in the clinic. Our aim was ...

    Authors: Katarzyna Ciaćma, Jerzy Więckiewicz, Sylwia Kędracka-Krok, Magdalena Kurtyka, Małgorzata Stec, Maciej Siedlar and Jarek Baran

    Citation: Microbial Cell Factories 2018 17:177

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  32. Content type: RESEARCH

    Economical production of value-added chemicals from renewable biomass is a promising path to sustainability. 3-Hydroxypropionic acid (3-HP) is an important chemical for building a bio-sustainable society. Esta...

    Authors: Seiya Takayama, Aiko Ozaki, Rie Konishi, Chisako Otomo, Mayumi Kishida, Yuuki Hirata, Takuya Matsumoto, Tsutomu Tanaka and Akihiko Kondo

    Citation: Microbial Cell Factories 2018 17:176

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  33. Content type: RESEARCH

    Autophagy is used for degradation of cellular components and nutrient recycling. Atg8 is one of the core proteins in autophagy and used as a marker for autophagic detection. However, the autophagy of filamento...

    Authors: Honghua Li, Pengjie Hu, Ying Wang, Yuanyuan Pan and Gang Liu

    Citation: Microbial Cell Factories 2018 17:175

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  34. Content type: Review

    Interest in bulk biomass from microalgae, for the extraction of high-value nutraceuticals, bio-products, animal feed and as a source of renewable fuels, is high. Advantages of microalgal vs. plant biomass prod...

    Authors: Manuel Benedetti, Valeria Vecchi, Simone Barera and Luca Dall’Osto

    Citation: Microbial Cell Factories 2018 17:173

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  35. Content type: RESEARCH

    Arbutin is a plant-derived glycoside with potential antioxidant, antibacterial and anti-inflammatory activities. Currently, it is mainly produced by plant extraction or enzymatic processes, which suffers from ...

    Authors: Songwei Wang, Cong Fu, Muhammad Bilal, Hongbo Hu, Wei Wang and Xuehong Zhang

    Citation: Microbial Cell Factories 2018 17:174

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  36. Content type: RESEARCH

    Proprotein convertase furin is responsible for the processing of a wide variety of precursors consisted of signal peptide, propeptide and mature peptide in mammal. Many precursors processed by furin have impor...

    Authors: Yide Huang, Yanyu Long, Suhuan Li, Ting Lin, Jingwen Wu, Yafei Zhang and Yao Lin

    Citation: Microbial Cell Factories 2018 17:172

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  37. Content type: RESEARCH

    The process of plant growth in the contaminated environment is often inhibited and entails the neutralization of harmful compounds. To reduce the negative impact of harmful compounds microorganisms produce uni...

    Authors: Olga Marchut-Mikolajczyk, Piotr Drożdżyński, Dominika Pietrzyk and Tadeusz Antczak

    Citation: Microbial Cell Factories 2018 17:171

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  38. Content type: Review

    Ammonium assimilation is linked to fundamental cellular processes that include the synthesis of non-essential amino acids like glutamate and glutamine. In Saccharomyces cerevisiae glutamate can be synthesized fro...

    Authors: P. Mara, G. S. Fragiadakis, F. Gkountromichos and D. Alexandraki

    Citation: Microbial Cell Factories 2018 17:170

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  39. Content type: RESEARCH

    Escherichia coli is one of the most widely used hosts for recombinant protein production in academia and industry. Strain BL21(DE3) is frequently employed due to its advantageous feature of lacking proteases whic...

    Authors: Johanna Hausjell, Julia Weissensteiner, Christian Molitor, Heidi Halbwirth and Oliver Spadiut

    Citation: Microbial Cell Factories 2018 17:169

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  40. Content type: RESEARCH

    Increasing efforts have been made to assess the potential of Escherichia coli strains for the production of complex recombinant proteins. Since a considerable part of therapeutic proteins are glycoproteins, the l...

    Authors: Phillipp Mueller, Rahul Gauttam, Nadja Raab, René Handrick, Claudia Wahl, Sebastian Leptihn, Michael Zorn, Michaela Kussmaul, Marianne Scheffold, Bernhard Eikmanns, Lothar Elling and Sabine Gaisser

    Citation: Microbial Cell Factories 2018 17:168

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  41. Content type: RESEARCH

    High thermostability is required for trypsin to have wider industrial applications. Target mutagenesis at flexible regions has been proved to be an efficient protein engineering method to enhance the protein t...

    Authors: Lin Liu, Haoran Yu, Kun Du, Zhiyan Wang, Yiru Gan and He Huang

    Citation: Microbial Cell Factories 2018 17:165

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  42. Content type: RESEARCH

    Cellular metabolism is tightly regulated by hard-wired multiple layers of biological processes to achieve robust and homeostatic states given the limited resources. As a result, even the most intuitive enzyme-...

    Authors: Lokanand Koduru, Meiyappan Lakshmanan and Dong-Yup Lee

    Citation: Microbial Cell Factories 2018 17:167

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  43. Content type: RESEARCH

    Microbial biofuel production provides a promising sustainable alternative to fossil fuels. 1-Butanol is recognized as an advanced biofuel and is gaining attention as an ideal green replacement for gasoline. In...

    Authors: Aiqun Yu, Yakun Zhao, Yaru Pang, Zhihui Hu, Cuiying Zhang, Dongguang Xiao, Matthew Wook Chang and Susanna Su Jan Leong

    Citation: Microbial Cell Factories 2018 17:166

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  44. Content type: RESEARCH

    Terpenes are an important and extremely versatile class of secondary metabolites that are commercially used in the pharmaceutical, food and cosmetics sectors. Genome mining of different fungal collections has ...

    Authors: Wolfgang Mischko, Max Hirte, Monika Fuchs, Norbert Mehlmer and Thomas B. Brück

    Citation: Microbial Cell Factories 2018 17:164

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  45. Content type: RESEARCH

    Bacillus subtilis has been widely used as a host for heterologous protein expression in food industry. B. subtilis ATCC6051 is an alternative expression host for the production of industrial enzymes, and exhibits...

    Authors: Xin Liu, Hai Wang, Bin Wang and Li Pan

    Citation: Microbial Cell Factories 2018 17:163

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  46. Content type: RESEARCH

    The fungus Ashbya gossypii is an important industrial producer of the vitamin riboflavin. Using this microbe, riboflavin is manufactured in a two-stage process based on a rich medium with vegetable oil, yeast ext...

    Authors: Susanne Katharina Schwechheimer, Judith Becker, Lindsay Peyriga, Jean-Charles Portais and Christoph Wittmann

    Citation: Microbial Cell Factories 2018 17:162

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  47. Content type: RESEARCH

    Proteins can be secreted from a host organism with the aid of N-terminal secretion signals. The budding yeast Pichia pastoris (Komagataella sp.) is widely employed to secrete proteins of academic and industrial i...

    Authors: Juan J. Barrero, Jason C. Casler, Francisco Valero, Pau Ferrer and Benjamin S. Glick

    Citation: Microbial Cell Factories 2018 17:161

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  48. Content type: RESEARCH

    The canonical heterotrimeric G protein-cAMP/PKA pathway regulates numerous cellular processes in filamentous fungi. Chaetomium globosum, a saprophytic fungus, is known for producing many secondary metabolites, in...

    Authors: Yang Hu, Yanjie Liu, Xiaoran Hao, Dan Wang, Oren Akhberdi, Biyun Xiang and Xudong Zhu

    Citation: Microbial Cell Factories 2018 17:160

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  49. Content type: RESEARCH

    Whole cells of Gluconobacter oxydans are widely used in various biocatalytic processes. Sorbitol at high concentrations is commonly used in complex media to prepare biocatalysts. Exploiting an alternative process...

    Authors: Jinxin Yan, Jing Xu, Menghao Cao, Zhong Li, Chengpeng Xu, Xinyu Wang, Chunyu Yang, Ping Xu, Chao Gao and Cuiqing Ma

    Citation: Microbial Cell Factories 2018 17:158

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  50. Content type: RESEARCH

    Microbial production of chemicals from renewable carbon sources enables a sustainable route to many bioproducts. Sugar streams, such as those derived from biomass pretreated with ionic liquids (IL), provide ef...

    Authors: Thomas Eng, Philipp Demling, Robin A. Herbert, Yan Chen, Veronica Benites, Joel Martin, Anna Lipzen, Edward E. K. Baidoo, Lars M. Blank, Christopher J. Petzold and Aindrila Mukhopadhyay

    Citation: Microbial Cell Factories 2018 17:159

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