Articles

Repression of mitochondrial metabolism for cytosolic pyruvate-derived chemical production in Saccharomyces cerevisiae

Saccharomyces cerevisiae is a suitable host for the industrial production of pyruvate-derived chemicals such as ethanol and 2,3-butanediol (23BD). For the improvement of the productivity of these chemicals, it is...

Authors: Keisuke Morita, Fumio Matsuda, Koji Okamoto, Jun Ishii, Akihiko Kondo and Hiroshi Shimizu

Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica

Yarrowia lipolytica is an unconventional yeast with a huge industrial potential. Despite many advantages for biotechnological applications, it possesses enormous demand for oxygen, which is a bottleneck in large ...

Authors: Aleksandra M. Mirończuk, Katarzyna E. Kosiorowska, Anna Biegalska, Magdalena Rakicka-Pustułka, Mateusz Szczepańczyk and Adam Dobrowolski

Exploring novel herbicidin analogues by transcriptional regulator overexpression and MS/MS molecular networking

Herbicidin F has an undecose tricyclic furano-pyrano-pyran structure with post-decorations. It was detected from Streptomyces mobaraensis US-43 fermentation broth as a trace component by HPLC–MS analysis. As herb...

Authors: Yuanyuan Shi, Renjie Gu, Yihong Li, Xinwei Wang, Weicong Ren, Xingxing Li, Lifei Wang, Yunying Xie and Bin Hong

Exploiting xylan as sugar donor for the synthesis of an antiproliferative xyloside using an enzyme cascade

Currently, industrial societies are seeking for green alternatives to conventional chemical synthesis. This demand has merged with the efforts to convert lignocellulosic biomass into value-added products. In t...

Authors: Manuel Nieto-Domínguez, José Alberto Martínez-Fernández, Beatriz Fernández de Toro, Juan A. Méndez-Líter, Francisco Javier Cañada, Alicia Prieto, Laura I. de Eugenio and María Jesús Martínez

Designer bioemulsifiers based on combinations of different polysaccharides with the novel emulsifying esterase AXE from Bacillus subtilis CICC 20034

Bioemulsifiers are surface-active compounds, which exhibit advantages including low toxicity, higher biodegradability and biocompatibility over synthetic chemical surfactants. Despite their potential benefits,...

Authors: Weiyi Tao, Junzhang Lin, Weidong Wang, He Huang and Shuang Li

Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides

α-Galactosidases are enzymes that act on galactosides present in many vegetables, mainly legumes and cereals, have growing importance with respect to our diet. For this reason, the use of their catalytic activ...

Authors: María-Efigenia Álvarez-Cao, María-Esperanza Cerdán, María-Isabel González-Siso and Manuel Becerra

Using a marine microalga as a chassis for polyethylene terephthalate (PET) degradation

The biological degradation of plastics is a promising method to counter the increasing pollution of our planet with artificial polymers and to develop eco-friendly recycling strategies. Polyethylene terephthal...

Authors: Daniel Moog, Johanna Schmitt, Jana Senger, Jan Zarzycki, Karl-Heinz Rexer, Uwe Linne, Tobias Erb and Uwe G. Maier

Metabolic perturbations in mutants of glucose transporters and their applications in metabolite production in Escherichia coli

Most microorganisms have evolved to maximize growth rate, with rapid consumption of carbon sources from the surroundings. However, fast growing phenotypes usually feature secretion of organic compounds. For ex...

Authors: Hwi-Min Jung, Dae-Kyun Im, Jae Hyung Lim, Gyoo Yeol Jung and Min-Kyu Oh

Construction of a novel MK-4 biosynthetic pathway in Pichia pastoris through heterologous expression of HsUBIAD1

With a variety of physiological and pharmacological functions, menaquinone is an essential prenylated product that can be endogenously converted from phylloquinone (VK₁) or menadione (VK₃) via the expression of H...

Authors: Xiaowen Sun, Hui Liu, Peng Wang, Li wang, Wenfeng Ni, Qiang Yang, Han Wang, Hengfang Tang, Genhai Zhao and Zhiming Zheng

Mevalonate production from ethanol by direct conversion through acetyl-CoA using recombinant Pseudomonas putida, a novel biocatalyst for terpenoid production

Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With t...

Authors: Jeongmo Yang, Ji Hee Son, Hyeonsoo Kim, Sukhyeong Cho, Jeong-geol Na, Young Joo Yeon and Jinwon Lee

Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions

The oleaginous yeast Yarrowia lipolytica is increasingly used as an alternative cell factory for the production of recombinant proteins. Recently, regulated promoters from genes EYK1 and EYD1, encoding an erythru...

Authors: Young-Kyoung Park, Marie Vandermies, Paul Soudier, Samuel Telek, Stéphane Thomas, Jean-Marc Nicaud and Patrick Fickers

In vivo immobilization of an organophosphorus hydrolyzing enzyme on bacterial polyhydroxyalkanoate nano-granules

Polyhydroxyalkanoate (PHA) are nano-granules naturally produced by bacteria. Two types of proteins, PHA synthase (PhaC) and phasins (PhaPs), are attached to the PHA surface by covalent and hydrophobic interact...

Authors: Ru Li, Jian Yang, Yunzhu Xiao and Lijuan Long

Optimal proteome allocation strategies for phototrophic growth in a light-limited chemostat

Authors: Marjan Faizi and Ralf Steuer

Biosynthesis of rhamnolipid by a Marinobacter species expands the paradigm of biosurfactant synthesis to a new genus of the marine microflora

In comparison to synthetically derived surfactants, biosurfactants produced from microbial culture are generally regarded by industry as being more sustainable and possess lower toxicity. One major class of bi...

Authors: Lakshmi Tripathi, Matthew S. Twigg, Aikaterini Zompra, Karina Salek, Victor U. Irorere, Tony Gutierrez, Georgios A. Spyroulias, Roger Marchant and Ibrahim M. Banat

Engineering Escherichia coli FAB system using synthetic plant genes for the production of long chain fatty acids

Sustainable production of microbial fatty acids derivatives has the potential to replace petroleum based equivalents in the chemical, cosmetic and pharmaceutical industry. Most fatty acid sources for productio...

Authors: Elias Kassab, Monika Fuchs, Martina Haack, Norbert Mehlmer and Thomas B. Brueck

Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis

Efficient and convenient genome-editing toolkits can expedite genomic research and strain improvement for desirable phenotypes. Zymomonas mobilis is a highly efficient ethanol-producing bacterium with a small gen...

Authors: Wei Shen, Jun Zhang, Binan Geng, Mengyue Qiu, Mimi Hu, Qing Yang, Weiwei Bao, Yubei Xiao, Yanli Zheng, Wenfang Peng, Guimin Zhang, Lixin Ma and Shihui Yang

Elevated CO₂ improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum

Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that e...

Authors: Songcui Wu, Wenhui Gu, Aiyou Huang, Yuanxiang Li, Manoj Kumar, Phaik Eem Lim, Li Huan, Shan Gao and Guangce Wang

Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory

Alpha-Terpineol (α-Terpineol), a C₁₀ monoterpenoid alcohol, is widely used in the cosmetic and pharmaceutical industries. Construction Saccharomyces cerevisiae cell factories for producing monoterpenes offers a p...

Authors: Chuanbo Zhang, Man Li, Guang-Rong Zhao and Wenyu Lu

Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33

Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database. Although multiple xylanases are de...

Authors: Yi Yang, Jinshui Yang, Ruonan Wang, Jiawen Liu, Yu Zhang, Liang Liu, Fengqin Wang and Hongli Yuan

Identification and optimization of PrsA in Bacillus subtilis for improved yield of amylase

PrsA is an extracytoplasmic folding catalyst essential in Bacillus subtilis. Overexpression of the native PrsA from B. subtilis has repeatedly lead to increased amylase yields. Nevertheless, little is known about...

Authors: Ane Quesada-Ganuza, Minia Antelo-Varela, Jeppe C. Mouritzen, Jürgen Bartel, Dörte Becher, Morten Gjermansen, Peter F. Hallin, Karen F. Appel, Mogens Kilstrup, Michael D. Rasmussen and Allan K. Nielsen

Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries

Antibody fragments can be expressed in Escherichia coli, where they are commonly directed to the periplasm via Sec pathway to enable disulphide bridge formations and correct folding. In order to transport antibod...

Authors: Antti Kulmala, Tuomas Huovinen and Urpo Lamminmäki

Adaptational changes in physiological and transcriptional responses of Bifidobacterium longum involved in acid stress resistance after successive batch cultures

Bifidobacterium inhabiting the human and animal intestinal tract is known for its health-promoting effect. Tolerance to acid stress is crucial for bifidobacteria to survive and then exert their beneficial effects...

Authors: Yanxia Wei, Jing Gao, Dianbin Liu, Yang Li and Wenli Liu

Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories

A microorganism engineered for non-native tasks may suffer stresses it never met before. Therefore, we examined whether a Kluyveromyces marxianus strain engineered with a carotenoid biosynthesis pathway can serve...

Authors: Hsien-Lin Liu, Jui-Jen Chang, Caroline Thia, Yu-Ju Lin, Shou-Chen Lo, Chieh-Chen Huang and Wen-Hsiung Li

¹³C metabolic flux analysis on roles of malate transporter in lipid accumulation of Mucor circinelloides

Mitochondrial and cytoplasmic malate transporter proteins are responsible for transmembrane transport of malate, thereby linking malate metabolism in various subcellular regions of the cell. These transporters...

Authors: Lu Wang, Huaiyuan Zhang, Yao Zhang and Yuanda Song

A systematic approach to study the pH-dependent release, productivity and product specificity of dextransucrases

Dextransucrases are extracellular enzymes, which catalyze the formation of α-1→6-linked glucose polymers from sucrose. These enzymes are exclusively expressed by lactic acid bacteria, which commonly acidify th...

Authors: Jonas Schmid, Julia Bechtner, Rudi F. Vogel and Frank Jakob

Engineering Pseudomonas putida for isoprenoid production by manipulating endogenous and shunt pathways supplying precursors

The soil bacterium Pseudomonas putida is a promising platform for the production of industrially valuable natural compounds. In the case of isoprenoids, the availability of biosynthetic precursors is a major limi...

Authors: Sofía Hernandez-Arranz, Jordi Perez-Gil, Dominic Marshall-Sabey and Manuel Rodriguez-Concepcion

Engineering protein production by rationally choosing a carbon and nitrogen source using E. coli BL21 acetate metabolism knockout strains

Escherichia coli (E. coli) is a bacteria that is widely employed in many industries for the production of high interest bio-products such as recombinant proteins. Nevertheless, the use of E. coli for recombinant ...

Authors: Gema Lozano Terol, Julia Gallego-Jara, Rosa Alba Sola Martínez, Manuel Cánovas Díaz and Teresa de Diego Puente

Metabolic fluxes-oriented control of bioreactors: a novel approach to tune micro-aeration and substrate feeding in fermentations

Fine-tuning the aeration for cultivations when oxygen-limited conditions are demanded (such as the production of vaccines, isobutanol, 2–3 butanediol, acetone, and bioethanol) is still a challenge in the area ...

Authors: Thiago José Barbosa Mesquita, Cíntia Regina Sargo, José Roberto Fuzer Neto, Sheyla Alexandra Hidalgo Paredes, Roberto de Campos Giordano, Antonio Carlos Luperni Horta and Teresa Cristina Zangirolami

Efficient simultaneous production of extracellular polyol esters of fatty acids and intracellular lipids from inulin by a deep-sea yeast Rhodotorula paludigena P4R5

Polyol esters of fatty acids (PEFA) are a kind of promising biosurfactants and mainly secreted by Rhodotorula strains. In addition, some strains of Rhodotorula are reliable producers of microbial lipid. Therefore...

Authors: Mengqi Wang, Weian Mao, Xiaoxiang Wang, Fengyi Li, Jiming Wang, Zhe Chi, Zhenming Chi and Guanglei Liu

TgSWO from Trichoderma guizhouense NJAU4742 promotes growth in cucumber plants by modifying the root morphology and the cell wall architecture

Colonization of Trichoderma spp. is essential for exerting their beneficial functions on the plant. However, the interactions between Trichoderma spp. and plant roots are still not completely understood. The aim ...

Authors: Xiaohui Meng, Youzhi Miao, Qiumei Liu, Lei Ma, Kai Guo, Dongyang Liu, Wei Ran and Qirong Shen

Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds

Poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) [P(3HB-co-3HHx)] is a bacterial polyester with high biodegradability, even in marine environments. Ralstonia eutropha has been engineered for the biosynthesis...

Authors: Mengxiao Zhang, Shunsuke Kurita, Izumi Orita, Satoshi Nakamura and Toshiaki Fukui

Metabolic engineering of roseoflavin-overproducing microorganisms

Roseoflavin, a promising broad-spectrum antibiotic, is naturally produced by the bacteria Streptomyces davaonensis and Streptomyces cinnabarinus. The key enzymes responsible for roseoflavin biosynthesis and the c...

Authors: Rodrigo Mora-Lugo, Julian Stegmüller and Matthias Mack

Xylose–glucose co-fermentation to ethanol by Escherichia coli strain MS04 using single- and two-stage continuous cultures under micro-aerated conditions

Simultaneous co-fermentation of mixed sugars is an important feature to consider in the production of ethanol from lignocellulosic biomass hydrolysates because it enhances the overall ethanol yield and volumet...

Authors: Marco T. Fernández-Sandoval, Juvencio Galíndez-Mayer, Francisco Bolívar, Guillermo Gosset, Octavio T. Ramírez and Alfredo Martinez

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia. Recently, it has been shown to be a potentiall...

Authors: Qi Liu, Xiaona Shi, Lili Song, Haifeng Liu, Xiangshan Zhou, Qiyao Wang, Yuanxing Zhang and Menghao Cai

A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations

In most microbial cultivations d-glucose is the main carbon and energy source. However, quantification of d-glucose especially in small scale is still challenging. Therefore, we developed a FRET-based glucose bio...

Authors: Julia Otten, Niklas Tenhaef, Roman P. Jansen, Johannes Döbber, Lisa Jungbluth, Stephan Noack, Marco Oldiges, Wolfgang Wiechert and Martina Pohl

Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)

Hirame novirhabdovirus (HIRRV) can infect a wide range of marine and freshwater fish, causing huge economic losses to aquaculture industry. Vaccine development, especially oral vaccine, has become an effective...

Authors: Lining Zhao, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing and Wenbin Zhan

Genetic variants of the oppA gene are involved in metabolic regulation of surfactin in Bacillus subtilis

Bacillus subtilis 916 has been identified as an effective biocontrol agent against Rhizoctonia solani, the causal pathogen of rice sheath blight, under greenhouse and field conditions. HPLC analysis showed that s...

Authors: Xiaoyu Wang, Zhiyi Chen, Hui Feng, Xi Chen and Lihui Wei

Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium

S-Formylglutathione is hydrolyzed to glutathione and formate by an S-formylglutathione hydrolase (SFGH) (3.1.2.12). This thiol esterase belongs to the esterase family and is also known as esterase D. SFGHs contai...

Authors: Chang Woo Lee, Wanki Yoo, Sun-Ha Park, Ly Thi Huong Luu Le, Chang-Sook Jeong, Bum Han Ryu, Seung Chul Shin, Han-Woo Kim, Hyun Park, Kyeong Kyu Kim, T. Doohun Kim and Jun Hyuck Lee

Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli

Acrylic acid (AA) is a widely used commodity chemical derived from non-renewable fossil fuel sources. Alternative microbial-based production methodologies are being developed with the aim of providing “green” ...

Authors: Sarada S. Raghavan, Sharon Chee, Juntao Li, Jeremie Poschmann, Niranjan Nagarajan, Siau Jia Wei, Chandra S. Verma and Farid J. Ghadessy

A GH51 α-l-arabinofuranosidase from Talaromyces leycettanus strain JCM12802 that selectively drives synergistic lignocellulose hydrolysis

The development of sustainable technologies for plant cell wall degradation greatly depends on enzymes with hydrolytic activities against carbohydrates. The waste by-products of agricultural cereals are import...

Authors: Tao Tu, Xiaoli Li, Kun Meng, Yingguo Bai, Yuan Wang, Zhenxing Wang, Bin Yao and Huiying Luo

Engineering actinomycetes for biosynthesis of macrolactone polyketides

Actinobacteria are characterized as the most prominent producer of natural products (NPs) with pharmaceutical importance. The production of NPs from these actinobacteria is associated with particular biosynthe...

Authors: Dipesh Dhakal, Jae Kyung Sohng and Ramesh Prasad Pandey

Enhanced acid-stress tolerance in Lactococcus lactis NZ9000 by overexpression of ABC transporters

Microbial cell factories are widely used in the production of acidic products such as organic acids and amino acids. However, the metabolic activity of microbial cells and their production efficiency are sever...

Authors: Zhengming Zhu, Jinhua Yang, Peishan Yang, Zhimeng Wu, Juan Zhang and Guocheng Du

Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers

Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their su...

Authors: Qianqian Zhuang and Qingsheng Qi

Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow,...

Authors: José M. Pastor, Nuno Borges, Juan P. Pagán, Sara Castaño-Cerezo, Laszlo N. Csonka, Bradley W. Goodner, Kathryn A. Reynolds, Luís G. Gonçalves, Montserrat Argandoña, Joaquín J. Nieto, Carmen Vargas, Vicente Bernal and Manuel Cánovas

Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts

Sufficient reference standards of drug metabolites are required in the drug discovery and development process. However, such drug standards are often expensive or not commercially available. Chemical synthesis...

Authors: Chongliang Gao and Tingjie Zheng

Metabolic engineering of Bacillus megaterium for heparosan biosynthesis using Pasteurella multocida heparosan synthase, PmHS2

Heparosan is the unsulfated precursor of heparin and heparan sulfate and its synthesis is typically the first step in the production of bioengineered heparin. In addition to its utility as the starting materia...

Authors: Asher Williams, Kamil S. Gedeon, Deepika Vaidyanathan, Yanlei Yu, Cynthia H. Collins, Jonathan S. Dordick, Robert J. Linhardt and Mattheos A. G. Koffas

Shaping the lipid composition of bacterial membranes for membrane protein production

The overexpression and purification of membrane proteins is a bottleneck in biotechnology and structural biology. E. coli remains the host of choice for membrane protein production. To date, most of the efforts h...

Authors: Kerstin Kanonenberg, Jorge Royes, Alexej Kedrov, Gereon Poschmann, Federica Angius, Audrey Solgadi, Olivia Spitz, Diana Kleinschrodt, Kai Stühler, Bruno Miroux and Lutz Schmitt

Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources in Escherichia coli

Acetyl-CoA is an important metabolic intermediate and serves as an acetylation precursor for the biosynthesis of various value-added acetyl-chemicals. Acetyl-CoA can be produced from glucose, acetate, or fatty...

Authors: Shasha Zhang, Wei Yang, Hao Chen, Bo Liu, Baixue Lin and Yong Tao

Long-chain vitamin K2 production in Lactococcus lactis is influenced by temperature, carbon source, aeration and mode of energy metabolism

Vitamin K2 (menaquinone, MK-n) is a lipid-soluble vitamin that functions as a carboxylase co-factor for maturation of proteins involved in many vital physiological processes in humans. Notably, long-chain vita...

Authors: Yue Liu, Eric O. van Bennekom, Yu Zhang, Tjakko Abee and Eddy J. Smid

Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate conversion ...

Authors: Xian Zhang, Rumeng Han, Teng Bao, Xiaojing Zhao, Xiangfei Li, Manchi Zhu, Taowei Yang, Meijuan Xu, Minglong Shao, Youxi Zhao and Zhiming Rao