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  1. Research

    Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

    The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and ...

    Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud and Christophe Leplat

    Microbial Cell Factories 2017 16:31

    Published on: 17 February 2017

  2. Research

    Thermus thermophilus as source of thermozymes for biotechnological applications: homologous expression and biochemical characterization of an α-galactosidase

    The genus Thermus, which has been considered for a long time as a fruitful source of biotechnological relevant enzymes, has emerged more recently as suitable host to overproduce thermozymes. Among these, α-galact...

    Martina Aulitto, Salvatore Fusco, Gabriella Fiorentino, Danila Limauro, Emilia Pedone, Simonetta Bartolucci and Patrizia Contursi

    Microbial Cell Factories 2017 16:28

    Published on: 13 February 2017

  3. Research

    A high-throughput expression screening platform to optimize the production of antimicrobial peptides

    Antimicrobial peptides (AMPs) are promising candidates for the development of novel antibiotics, but it is difficult to produce sufficient quantities for preclinical and clinical studies due to their toxicity ...

    Christine Schreiber, Hagen Müller, Oliver Birrenbach, Moritz Klein, Doreen Heerd, Tobias Weidner, Denise Salzig and Peter Czermak

    Microbial Cell Factories 2017 16:29

    Published on: 13 February 2017

  4. Research

    Secretion of biologically active pancreatitis-associated protein I (PAP) by genetically modified dairy Lactococcus lactis NZ9000 in the prevention of intestinal mucositis

    Mucositis is one of the most relevant gastrointestinal inflammatory conditions in humans, generated by the use of chemotherapy drugs, such as 5-fluoracil (5-FU). 5-FU-induced mucositis affects 80% of patients ...

    Rodrigo D. Carvalho, Natalia Breyner, Zelia Menezes-Garcia, Nubia M. Rodrigues, Luisa Lemos, Tatiane U. Maioli, Danielle da Gloria Souza, Denise Carmona, Ana M. C. de Faria, Philippe Langella, Jean-Marc Chatel, Luis G. Bermúdez-Humarán, Henrique C. P. Figueiredo, Vasco Azevedo and Marcela S. de Azevedo

    Microbial Cell Factories 2017 16:27

    Published on: 13 February 2017

  5. Research

    Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides

    Microalgae have the potential to rapidly accumulate lipids of high interest for the food, cosmetics, pharmaceutical and energy (e.g. biodiesel) industries. However, current lipid extraction methods show effic...

    Xiaojie Ren, Xinhe Zhao, François Turcotte, Jean-Sébastien Deschênes, Réjean Tremblay and Mario Jolicoeur

    Microbial Cell Factories 2017 16:26

    Published on: 11 February 2017

  6. Research

    Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

    Poly-gamma-glutamic acid (γ-PGA) is a promising macromolecule with potential as a replacement for chemosynthetic polymers. γ-PGA can be produced by many microorganisms, including Bacillus species. Bacillus lichen...

    Wencheng Yu, Zhen Chen, Hong Ye, Peize Liu, Zhipeng Li, Yuanpeng Wang, Qingbiao Li, Shan Yan, Chuan-jian Zhong and Ning He

    Microbial Cell Factories 2017 16:22

    Published on: 8 February 2017

  7. Research

    SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

    The yeast Saccharomyces cerevisiae is increasingly used as a cell factory. However, cell factory construction time is a major obstacle towards using yeast for bio-production. Hence, tools to speed up cell factory...

    Katherina García Vanegas, Beata Joanna Lehka and Uffe Hasbro Mortensen

    Microbial Cell Factories 2017 16:25

    Published on: 8 February 2017

  8. Research

    Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens

    The halophilic bacterium Chromohalobacter salexigens has been proposed as promising cell factory for the production of the compatible solutes ectoine and hydroxyectoine. This bacterium has evolved metabolic adapt...

    María J. Salar-García, Vicente Bernal, José M. Pastor, Manuel Salvador, Montserrat Argandoña, Joaquín J. Nieto, Carmen Vargas and Manuel Cánovas

    Microbial Cell Factories 2017 16:23

    Published on: 8 February 2017

  9. Research

    Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

    Droplet-based microfluidics is becoming an increasingly attractive alternative to microtiter plate techniques for enzymatic high-throughput screening (HTS), especially for exploring large diversities with lowe...

    Thomas Beneyton, Stéphane Thomas, Andrew D. Griffiths, Jean-Marc Nicaud, Antoine Drevelle and Tristan Rossignol

    Microbial Cell Factories 2017 16:18

    Published on: 31 January 2017

  10. Research

    Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

    Through functional screening of a fosmid library, generated from a phytopathogen-suppressive soil metagenome, the novel antifungal chitinase—named Chi18H8 and belonging to family 18 glycosyl hydrolases—was pre...

    Francesca Berini, Ilaria Presti, Fabrizio Beltrametti, Marco Pedroli, Kjell M. Vårum, Loredano Pollegioni, Sara Sjöling and Flavia Marinelli

    Microbial Cell Factories 2017 16:16

    Published on: 31 January 2017

  11. Research

    Dynamic control of ERG20 expression combined with minimized endogenous downstream metabolism contributes to the improvement of geraniol production in Saccharomyces cerevisiae

    Microbial production of monoterpenes provides a promising substitute for traditional chemical-based methods, but their production is lagging compared with sesquiterpenes. Geraniol, a valuable monoterpene alcoh...

    Jianzhi Zhao, Chen Li, Yan Zhang, Yu Shen, Jin Hou and Xiaoming Bao

    Microbial Cell Factories 2017 16:17

    Published on: 31 January 2017

  12. Review

    Microbial production of vitamin B12: a review and future perspectives

    Vitamin B12 is an essential vitamin that is widely used in medical and food industries. Vitamin B12 biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentatio...

    Huan Fang, Jie Kang and Dawei Zhang

    Microbial Cell Factories 2017 16:15

    Published on: 30 January 2017

  13. Research

    Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesis

    Camelids possess unique functional heavy chain antibodies, which can be produced and modified in vitro as a single domain antibody (sdAb or nanobody) with full antigen binding ability. Production of sdAb in co...

    Lubos Comor, Saskia Dolinska, Katarina Bhide, Lucia Pulzova, Irene Jiménez-Munguía, Elena Bencurova, Zuzana Flachbartova, Lenka Potocnakova, Evelina Kanova and Mangesh Bhide

    Microbial Cell Factories 2017 16:13

    Published on: 23 January 2017

  14. Research

    Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter

    Baker’s yeast Saccharomyces cerevisiae is a proven host for the commercial production of recombinant biopharmaceutical proteins. For the manufacture of heterologous proteins with activities deleterious to the hos...

    Thea S. B. Møller, Joanna Hay, Malcolm J. Saxton, Karen Bunting, Evamaria I. Petersen, Søren Kjærulff and Christopher J. A. Finnis

    Microbial Cell Factories 2017 16:11

    Published on: 18 January 2017

  15. Research

    Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis

    Milbemycins, produced from Streptomyces hygroscopicus subsp. aureolacrimosus and Streptomyces bingchenggensis, are 16-membered macrolides that share structural similarity with avermectin produced from Streptomyce...

    Myoun-Su Kim, Wan-Je Cho, Myoung Chong Song, Seong-Whan Park, Kaeun Kim, Eunji Kim, Naryeong Lee, Sang-Jip Nam, Ki-Hoon Oh and Yeo Joon Yoon

    Microbial Cell Factories 2017 16:9

    Published on: 17 January 2017

  16. Research

    Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

    Animal venoms are large, complex libraries of bioactive, disulphide-rich peptides. These peptides, and their novel biological activities, are of increasing pharmacological and therapeutic importance. However, ...

    Ana Filipa Sequeira, Jeremy Turchetto, Natalie J. Saez, Fanny Peysson, Laurie Ramond, Yoan Duhoo, Marilyne Blémont, Vânia O. Fernandes, Luís T. Gama, Luís M. A. Ferreira, Catarina I. P. I. Guerreiro, Nicolas Gilles, Hervé Darbon, Carlos M. G. A. Fontes and Renaud Vincentelli

    Microbial Cell Factories 2017 16:4

    Published on: 17 January 2017

  17. Research

    CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

    Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins ...

    Brady F. Cress, Quentin D. Leitz, Daniel C. Kim, Teresita D. Amore, Jon Y. Suzuki, Robert J. Linhardt and Mattheos A. G. Koffas

    Microbial Cell Factories 2017 16:10

    Published on: 17 January 2017

  18. Research

    High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

    Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity and efficacy, a variety of membrane receptors. Di...

    Jeremy Turchetto, Ana Filipa Sequeira, Laurie Ramond, Fanny Peysson, Joana L. A. Brás, Natalie J. Saez, Yoan Duhoo, Marilyne Blémont, Catarina I. P. D. Guerreiro, Loic Quinton, Edwin De Pauw, Nicolas Gilles, Hervé Darbon, Carlos M. G. A. Fontes and Renaud Vincentelli

    Microbial Cell Factories 2017 16:6

    Published on: 17 January 2017

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