Articles

Gut microbiome as a response marker for pancreatic enzyme replacement therapy in a porcine model of exocrine pancreas insufficiency

Exocrine pancreatic insufficiency (EPI) is characterized by the loss of active pancreatic enzymes and a resulting severely reduced food digestion. EPI therapy requires orally applied pancreatic enzyme replacem...

Authors: Sabrina Ritz, Daniela Hahn, Haleluya T. Wami, Karin Tegelkamp, Ulrich Dobrindt and Juergen Schnekenburger

The generation of metabolic changes for the production of high-purity zeaxanthin mediated by CRISPR-Cas9 in Chlamydomonas reinhardtii

Zeaxanthin, a major xanthophyll pigment, has a significant role as a retinal pigment and antioxidant. Because zeaxanthin helps to prevent age-related macular degeneration, its commercial use in personalized nu...

Authors: Inhwa Song, Jongrae Kim, Kwangryul Baek, Young Choi, ByongCheol Shin and EonSeon Jin

Effect of diet and intestinal AhR expression on fecal microbiome and metabolomic profiles

Diet, loss of aryl hydrocarbon receptor (AhR) expression and their modification of the gut microbiota community composition and its metabolites affect the development of colorectal cancer (CRC). However, the c...

Authors: Fang Yang, Jennifer A. A. DeLuca, Rani Menon, Erika Garcia-Vilarato, Evelyn Callaway, Kerstin K. Landrock, Kyongbum Lee, Stephen H. Safe, Robert S. Chapkin, Clinton D. Allred and Arul Jayaraman

Metabolic engineering of Saccharomyces cerevisiae for high-level production of gastrodin from glucose

The natural phenolic glycoside gastrodin is the major bioactive ingredient in the well-known Chinese herb Tianma and is widely used as a neuroprotective medicine in the clinic. Microbial production from sustai...

Authors: Hua Yin, Tiandong Hu, Yibin Zhuang and Tao Liu

Dual and mutual interaction between microbiota and viral infections: a possible treat for COVID-19

All of humans and other mammalian species are colonized by some types of microorganisms such as bacteria, archaea, unicellular eukaryotes like fungi and protozoa, multicellular eukaryotes like helminths, and v...

Authors: Taha Baghbani, Hossein Nikzad, Javid Azadbakht, Fatemeh Izadpanah and Hamed Haddad Kashani

Efficient protection of microorganisms for delivery to the intestinal tract by cellulose sulphate encapsulation

Gut microbiota in humans and animals play an important role in health, aiding in digestion, regulation of the immune system and protection against pathogens. Changes or imbalances in the gut microbiota (dysbio...

Authors: Walter H. Gunzburg, Myo Myint Aung, Pauline Toa, Shirelle Ng, Eliot Read, Wee Jin Tan, Eva Maria Brandtner, John Dangerfield and Brian Salmons

TLR-2 expression and dysregulated human Treg/Th17 phenotype in Aspergillus flavus infected patients of chronic rhinosinusitis with nasal polyposis

T helper (Th)17 and regulatory T (Treg) cells with toll-like receptor (TLR)-2 have been acknowledged to play a critical role in chronic rhinosinusitis with nasal polyposis (CRSwNP). However, its pathogenesis h...

Authors: Gargi Rai, Shukla Das, Mohammad Ahmad Ansari, Praveen Kumar Singh, Sajad Ahmad Dar, Shafiul Haque, Neelima Gupta, Sonal Sharma, Vishnampettai Ganapathysubramanian Ramachandran, Sanskriti Sharma, Charu Jain and Shipra Sharma

Isolation, heterologous expression, and purification of a novel antifungal protein from Bacillus subtilis strain Z-14

Wheat sheath blight, a soil borne fungal disease caused by Rhizoctonia cerealis, is considered as one of the most serious threats to wheat worldwide. Bacillus subtilis Z-14 was isolated from soil sampled from a w...

Authors: Xuechao Zhang, Xiaojun Guo, Cuihong Wu, Chengcui Li, Dongdong Zhang and Baocheng Zhu

Metabolite secretions of Lactobacillus plantarum YYC-3 may inhibit colon cancer cell metastasis by suppressing the VEGF-MMP2/9 signaling pathway

Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress ...

Authors: Yuan-Chun Yue, Bao-Yu Yang, Jing Lu, Shu-Wen Zhang, Liu Liu, Khaled Nassar, Xiao-Xi Xu, Xiao-Yang Pang and Jia-Ping Lv

Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of...

Authors: Sihan Li, Youcheng He, Haiou Zhang, Rong Zheng, Ruoying Xu, Qihong Liu, Shuihua Tang, Xiao Ke and Minghan Huang

Different transcriptional responses of haploid and diploid S. cerevisiae strains to changes in cofactor preference of XR

Xylitol accumulation is a major barrier for efficient ethanol production through heterologous xylose reductase-xylitol dehydrogenase (XR-XDH) pathway in recombinant Saccharomyces cerevisiae. Mutated NADH-preferri...

Authors: Cai-Yun Xie, Bai-Xue Yang, Qing-Ran Song, Zi-Yuan Xia, Min Gou and Yue-Qin Tang

Current insights into the relationship between the gut microbiome and Sjögren’s syndrome

Authors: Taco A. van der Meulen, Frans G. M. Kroese, Hendrika Bootsma, Fred K. L. Spijkervet and Arjan Vissink

Engineering nature for gaseous hydrocarbon production

The development of sustainable routes to the bio-manufacture of gaseous hydrocarbons will contribute widely to future energy needs. Their realisation would contribute towards minimising over-reliance on fossil...

Authors: Mohamed Amer, Helen Toogood and Nigel S. Scrutton

Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks

In an effort to ensure future energy security, reduce greenhouse gas emissions and create domestic jobs, the US has invested in technologies to develop sustainable biofuels and bioproducts from renewable carbo...

Authors: Gina M. Geiselman, James Kirby, Alexander Landera, Peter Otoupal, Gabriella Papa, Carolina Barcelos, Eric R. Sundstrom, Lalitendu Das, Harsha D. Magurudeniya, Maren Wehrs, Alberto Rodriguez, Blake A. Simmons, Jon K. Magnuson, Aindrila Mukhopadhyay, Taek Soon Lee, Anthe George…

Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products

The yeast Kluyveromyces marxianus offers unique potential for industrial biotechnology because of useful features like rapid growth, thermotolerance and a wide substrate range. As an emerging alternative platform...

Authors: Arun S. Rajkumar and John P. Morrissey

An automated oxystat fermentation regime for microoxic cultivation of Magnetospirillum gryphiswaldense

Magnetosomes produced by magnetotactic bacteria represent magnetic nanoparticles with unprecedented characteristics. However, their use in many biotechnological applications has so far been hampered by their c...

Authors: Cornelius N. Riese, René Uebe, Sabine Rosenfeldt, Anna S. Schenk, Valérie Jérôme, Ruth Freitag and Dirk Schüler

Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis

Plipastatin is a potent Bacillus antimicrobial lipopeptide with the prospect to replace conventional antifungal chemicals for controlling plant pathogens. However, the application of this lipopeptide has so far b...

Authors: Maliheh Vahidinasab, Lars Lilge, Aline Reinfurt, Jens Pfannstiel, Marius Henkel, Kambiz Morabbi Heravi and Rudolf Hausmann

Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production

Lipids from oleaginous yeasts emerged as a sustainable alternative to vegetable oils and animal fat to produce biodiesel, the biodegradable and environmentally friendly counterpart of petro-diesel fuel. To dev...

Authors: Francesca Martani, Letizia Maestroni, Mattia Torchio, Diletta Ami, Antonino Natalello, Marina Lotti, Danilo Porro and Paola Branduardi

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

Human vagina is colonised by a diverse array of microorganisms that make up the normal microbiota and mycobiota. Lactobacillus is the most frequently isolated microorganism from the healthy human vagina, this inc...

Authors: Wallace Jeng Yang Chee, Shu Yih Chew and Leslie Thian Lung Than

Intelligent microbial cell factory with genetic pH shooting (GPS) for cell self-responsive base/acid regulation

In industrial fermentation, pH fluctuation resulted from microbial metabolism influences the strain performance and the final production. The common way to control pH is adding acid or alkali after probe detec...

Authors: Chenyi Li, Xiaopeng Gao, Xiao Peng, Jinlin Li, Wenxin Bai, Jiadong Zhong, Mengchao He, Ke Xu, Ying Wang and Chun Li

Challenging microalgal vitamins for human health

Vitamins’ deficiency in humans is an important threat worldwide and requires solutions. In the concept of natural biofactory for bioactive compounds production, microalgae represent one of the most promising t...

Authors: Angelo Del Mondo, Arianna Smerilli, Elisabet Sané, Clementina Sansone and Christophe Brunet

Enhanced production of taxadiene in Saccharomyces cerevisiae

Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol^®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in ...

Authors: Behnaz Nowrouzi, Rachel A. Li, Laura E. Walls, Leo d’Espaux, Koray Malcı, Lungang Liang, Nestor Jonguitud-Borrego, Albert I. Lerma-Escalera, Jose R. Morones-Ramirez, Jay D. Keasling and Leonardo Rios-Solis

Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

The methylotrophic yeast, Pichia pastoris has been widely used for the production of human therapeutics, but production of granulocyte colony-stimulating factor (G-CSF) in this yeast is low.The work reported here...

Authors: Sakshi Aggarwal and Saroj Mishra

Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger

Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamyla...

Authors: Yu-fei Sui, Tabea Schütze, Li-ming Ouyang, Hongzhong Lu, Peng Liu, Xianzun Xiao, Jie Qi, Ying-Ping Zhuang and Vera Meyer

Improved methods for mass production of magnetosomes and applications: a review

Magnetotactic bacteria have the unique ability to synthesize magnetosomes (nano-sized magnetite or greigite crystals arranged in chain-like structures) in a variety of shapes and sizes. The chain alignment of ...

Authors: Abdul Basit, Jiaojiao Wang, Fangfang Guo, Wei Niu and Wei Jiang

MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

In Saccharomyces cerevisiae, alpha-glucosidase (maltase) is a key enzyme in maltose metabolism. In addition, the overexpression of the alpha-glucosidase-encoding gene MAL62 has been shown to increase the freezing...

Authors: Xi Sun, Jun Zhang, Zhi-Hua Fan, Ping Xiao, Feng Li, Hai-Qing Liu and Wen-Bi Zhu

Enhancing the insecticidal activity of new Bacillus thuringiensis X023 by copper ions

A new Bacillus thuringiensis X023 (BtX023) with high insecticidal activity was isolated in Hunan Province, China. The addition of metals (Cu, Fe, Mg and Mn) to the medium could influence the formation of spores a...

Authors: Zhuolin Liu, Junyan Xie, Ziru Deng, Mulan Wang, Dandan Dang, Sha Luo, Yunfeng Wang, Yunjun Sun, Liqiu Xia and Xuezhi Ding

Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis

Enzymatic quantification of creatinine has become an essential method for clinical evaluation of renal function. Although creatinase (CR) is frequently used for this purpose, its poor thermostability severely ...

Authors: Xue Bai, Daixi Li, Fuqiang Ma, Xi Deng, Manjie Luo, Yan Feng and Guangyu Yang

Haemagglutinin displayed on the surface of Lactococcus lactis confers broad cross-clade protection against different H5N1 viruses in chickens

The highly pathogenic avian influenza (HPAI) H5N1 virus poses a potential threat to the poultry industry. The currently available avian influenza H5N1 vaccines for poultry are clade-specific. Therefore, an eff...

Authors: Han Lei, Tong Gao, Qianhong Cen and Xiaojue Peng

Correction to: Gut microbial characteristics of adult patients with allergy rhinitis

An amendment to this paper has been published and can be accessed via the original article.

Authors: Libing Zhu, Feng Xu, Wenrong Wan, Bin Yu, Lin Tang, Yimin Yang, Yanling Du, Zhangran Chen and Hongzhi Xu

Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJΔ1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4

Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cel...

Authors: Weiwei Dong, Yuyuan Cai, Zhilong Xu, Biao Fu, Qitong Chen, Yuxin Cui, Zhiyong Ruan, Yunxiang Liang, Nan Peng and Shumiao Zhao

Construction of a new T7 promoter compatible Escherichia coli Nissle 1917 strain for recombinant production of heme-dependent proteins

Heme proteins and heme-derived molecules are essential in numerous cellular processes. Research into their in vitro functionality requires the production of large amounts of protein. Unfortunately, high yield ...

Authors: Kerstin Fiege and Nicole Frankenberg-Dinkel

MsrR is a thiol-based oxidation-sensing regulator of the XRE family that modulates C. glutamicum oxidative stress resistance

Corynebacterium glutamicum thrives under oxidative stress caused by the inevitably extreme environment during fermentation as it harbors antioxidative stress genes. Antioxidant genes are controlled by pathway-spe...

Authors: Meiru Si, Can Chen, Jingyi Zhong, Xiaona Li, Yang Liu, Tao Su and Ge Yang

Catabolism of l-rhamnose in A. nidulans proceeds via the non-phosphorylated pathway and is glucose repressed by a CreA-independent mechanism

l-rhamnose (6-deoxy-mannose) occurs in nature mainly as a component of certain plant structural polysaccharides and bioactive metabolites but has also been found in some microorganisms and animals. The release of...

Authors: Andrew P. MacCabe, Elpinickie I. Ninou, Ester Pardo and Margarita Orejas

Whole-genome and enzymatic analyses of an androstenedione-producing Mycobacterium strain with residual phytosterol-degrading pathways

Mycobacterium neoaurum strains can transform phytosterols to 4-androstene-3,17-dione (4-AD), a key intermediate for the synthesis of advanced steroidal medicines. In this work, we presented the complete genome se...

Authors: Hongwei Wang, Shikui Song, Fei Peng, Fei Yang, Tian Chen, Xin Li, Xiyao Cheng, Yijun He, Yongqi Huang and Zhengding Su

Immunogenicity evaluation of recombinant Lactobacillus casei W56 expressing bovine viral diarrhea virus E2 protein in conjunction with cholera toxin B subunit as an adjuvant

Bovine viral diarrhea virus (BVDV) is one of the main causes of infectious diseases in cattle and causes large financial losses to the cattle industry worldwide. In this study, Lactobacillus casei strain W56 (Lc ...

Authors: Shuo Jia, Xinning Huang, Hua Li, Dianzhong Zheng, Li Wang, Xinyuan Qiao, Yanping Jiang, Wen Cui, Lijie Tang, Yijing Li and Yigang Xu

The temperature of growth and sporulation modulates the efficiency of spore-display in Bacillus subtilis

Bacterial spores displaying heterologous antigens or enzymes have long been proposed as mucosal vaccines, functionalized probiotics or biocatalysts. Two main strategies have been developed to display heterolog...

Authors: Claudia Petrillo, Stefany Castaldi, Mariamichela Lanzilli, Anella Saggese, Giuliana Donadio, Loredana Baccigalupi, Ezio Ricca and Rachele Isticato

Production and identification of two antifungal terpenoids from the Posidonia oceanica epiphytic Ascomycota Mariannaea humicola IG100

Marine fungi are an important repository of bioactive molecules with great potential in different technological fields, the annual number of new compounds isolated from marine fungi is impressive and the gener...

Authors: Lorenzo Botta, Raffaele Saladino, Paolo Barghini, Massimiliano Fenice and Marcella Pasqualetti

Purification and initial characterization of Plasmodium falciparum K⁺ channels, PfKch1 and PfKch2 produced in Saccharomyces cerevisiae

Resistance towards known antimalarial drugs poses a significant problem, urging for novel drugs that target vital proteins in the malaria parasite Plasmodium falciparum. However, recombinant production of malaria...

Authors: Karen Molbaek, Maria Tejada, Christina Hoeier Ricke, Peter Scharff-Poulsen, Peter Ellekvist, Claus Helix-Nielsen, Nirbhay Kumar, Dan A. Klaerke and Per Amstrup Pedersen

How fructophilic lactic acid bacteria may reduce the FODMAPs content in wheat-derived baked goods: a proof of concept

FODMAPs (Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols) intake is associated with the onset of irritable bowel syndrome symptoms. FODMAPs in wheat-derived baked goods may be reduced...

Authors: Marta Acín Albiac, Raffaella Di Cagno, Pasquale Filannino, Vincenzo Cantatore and Marco Gobbetti

Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacill...

Authors: Wen Song, Nuo Zhang, Mo Yang, Yuling Zhou, Nisha He and Guimin Zhang

Heterologous redox partners supporting the efficient catalysis of epothilone B biosynthesis by EpoK in Schlegelella brevitalea

Epothilone B is a natural product that stabilizes microtubules, similar to paclitaxel (Taxol); therefore, epothilone B and several derivatives have shown obvious antitumour activities. Some of these products a...

Authors: Junheng Liang, Huimin Wang, Xiaoying Bian, Youming Zhang, Guoping Zhao and Xiaoming Ding

Comparative metabolomics of Phialemonium curvatum as an omnipotent fungus cultivated on crude palm oil versus glucose

Sugars and triglycerides are common carbon sources for microorganisms. Nonetheless, a systematic comparative interpretation of metabolic changes upon vegetable oil or glucose as sole carbon source is still lac...

Authors: Arief Izzairy Zamani, Susann Barig, Sarah Ibrahim, Hirzun Mohd. Yusof, Julia Ibrahim, Jaime Yoke Sum Low, Shwu Fun Kua, Syarul Nataqain Baharum, Klaus-Peter Stahmann and Chyan Leong Ng

In vivo characterization of the activities of novel cyclodipeptide oxidases: new tools for increasing chemical diversity of bioproduced 2,5-diketopiperazines in Escherichia coli

Cyclodipeptide oxidases (CDOs) are enzymes involved in the biosynthesis of 2,5-diketopiperazines, a class of naturally occurring compounds with a large range of pharmaceutical activities. CDOs belong to cyclod...

Authors: Fabien Le Chevalier, Isabelle Correia, Lucrèce Matheron, Morgan Babin, Mireille Moutiez, Nicolas Canu, Muriel Gondry, Olivier Lequin and Pascal Belin

Correction to: Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies

An amendment to this paper has been published and can be accessed via the original article.

Authors: Yunchang Xie, Jiawen Chen, Bo Wang, Tai Chen, Junyu Chen, Yuan Zhang, Xiaoying Liu and Qi Chen

Inducible intracellular membranes: molecular aspects and emerging applications

Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de...

Authors: Jorge Royes, Valérie Biou, Nathalie Dautin, Christophe Tribet and Bruno Miroux

Exploring the use of leucine zippers for the generation of a new class of inclusion bodies for pharma and biotechnological applications

Inclusion bodies (IBs) are biologically active protein aggregates forming natural nanoparticles with a high stability and a slow-release behavior. Because of their nature, IBs have been explored to be used as ...

Authors: Ramon Roca-Pinilla, Sara Fortuna, Antonino Natalello, Alejandro Sánchez-Chardi, Diletta Ami, Anna Arís and Elena Garcia-Fruitós

CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coli

One major mission of microbial breeding is high-level production of desired metabolites. Overproduction of intermediate metabolites in core pathways is challenging as it may impair cell growth and viability.

Authors: Qingyang Li, Peng Zhao, Hang Yin, Zhaonan Liu, Haifeng Zhao and Pingfang Tian

Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine

Due to its clear inherited backgrounds as well as simple and diverse genetic manipulation systems, Bacillus subtilis is the key Gram-positive model bacterium for studies on physiology and metabolism. Furthermore,...

Authors: Yuan Su, Chuan Liu, Huan Fang and Dawei Zhang

Application of different types of CRISPR/Cas-based systems in bacteria

As important genome editing tools, CRISPR/Cas systems, especially those based on type II Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria. However, the intrinsic toxicit...

Authors: Zhenquan Liu, Huina Dong, Yali Cui, Lina Cong and Dawei Zhang