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  1. Research

    Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes

    The strategy of modifying the sterol catabolism pathway in mycobacteria has been adopted to produce steroidal pharmaceutical intermediates, such as 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC), which is use...

    Liang-Bin Xiong, Hao-Hao Liu, Li-Qin Xu, Wan-Ju Sun, Feng-Qing Wang and Dong-Zhi Wei

    Microbial Cell Factories 2017 16:89

    Published on: 22 May 2017

  2. Research

    Enhancing poly-γ-glutamic acid production in Bacillus amyloliquefaciens by introducing the glutamate synthesis features from Corynebacterium glutamicum

    Poly-γ-glutamic acid (γ-PGA) is a valuable polymer with glutamate as its sole precursor. Enhancement of the intracellular glutamate synthesis is a very important strategy for the improvement of γ-PGA productio...

    Jun Feng, Yufen Quan, Yanyan Gu, Fenghong Liu, Xiaozhong Huang, Haosheng Shen, Yulei Dang, Mingfeng Cao, Weixia Gao, Xiaoyun Lu, Yi Wang, Cunjiang Song and Shufang Wang

    Microbial Cell Factories 2017 16:88

    Published on: 22 May 2017

  3. Research

    Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli

    Cyclodextrin glucanotransferases (CGTases) catalyze the synthesis of cyclodextrins, cyclic oligosaccharides composed of glucose monomers that find applications in the pharmaceutical, food, and cosmetic industr...

    Christian Sonnendecker, Ren Wei, Elisabeth Kurze, Jinpeng Wang, Thorsten Oeser and Wolfgang Zimmermann

    Microbial Cell Factories 2017 16:87

    Published on: 19 May 2017

  4. Research

    The effect of hypoxia on the lipidome of recombinant Pichia pastoris

    Cultivation of recombinant Pichia pastoris (Komagataella sp.) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expres...

    Núria Adelantado, Pablo Tarazona, Karlheinz Grillitsch, Xavier García-Ortega, Sergi Monforte, Francisco Valero, Ivo Feussner, Günther Daum and Pau Ferrer

    Microbial Cell Factories 2017 16:86

    Published on: 19 May 2017

  5. Research

    Production of a recombinant swollenin from Trichoderma harzianum in Escherichia coli and its potential synergistic role in biomass degradation

    Fungal swollenins (SWOs) constitute a class of accessory proteins that are homologous to canonical plant expansins. Expansins and expansin-related proteins are well known for acting in the deagglomeration of c...

    Clelton A. Santos, Jaire A. Ferreira-Filho, Anthonia O’Donovan, Vijai K. Gupta, Maria G. Tuohy and Anete P. Souza

    Microbial Cell Factories 2017 16:83

    Published on: 16 May 2017

  6. Research

    Purification and properties of a novel quizalofop-p-ethyl-hydrolyzing esterase involved in quizalofop-p-ethyl degradation by Pseudomonas sp. J-2

    Quizalofop-p-ethyl (QPE) is a post-emergence herbicide that effectively controls grass weeds and is often detected in the environment. However, the biochemical and molecular mechanisms of QPE degradation in the e...

    Hui Zhang, Mengya Li, Jie Li, Guangli Wang and Yuan Liu

    Microbial Cell Factories 2017 16:80

    Published on: 10 May 2017

  7. Research

    Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses

    Unlike the well-studied backer yeast where catabolite repression represents a burden for mixed substrate fermentation, Yarrowia lipolytica, an oleaginous yeast, is recognized for its potential to produce single c...

    Wael Sabra, Rajesh Reddy Bommareddy, Garima Maheshwari, Seraphim Papanikolaou and An-Ping Zeng

    Microbial Cell Factories 2017 16:78

    Published on: 8 May 2017

  8. Review

    Beneficial effects on host energy metabolism of short-chain fatty acids and vitamins produced by commensal and probiotic bacteria

    The aim of this review is to summarize the effect in host energy metabolism of the production of B group vitamins and short chain fatty acids (SCFA) by commensal, food-grade and probiotic bacteria, which are a...

    Jean Guy LeBlanc, Florian Chain, Rebeca Martín, Luis G. Bermúdez-Humarán, Stéphanie Courau and Philippe Langella

    Microbial Cell Factories 2017 16:79

    Published on: 8 May 2017

  9. Research

    Heterologous expression of abaecin peptide from Apis mellifera in Pichia pastoris

    Antimicrobial peptides (AMPs) are the first line of host immune defense against pathogens. Among AMPs from the honeybee Apis mellifera, abaecin is a major broad-spectrum antibacterial proline-enriched cationic pe...

    Denis Prudencio Luiz, Juliana Franco Almeida, Luiz Ricardo Goulart, Nilson Nicolau-Junior and Carlos Ueira-Vieira

    Microbial Cell Factories 2017 16:76

    Published on: 3 May 2017

  10. Research

    Purification and characterization of a novel cold adapted fungal glucoamylase

    Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amyl...

    Mario Carrasco, Jennifer Alcaíno, Víctor Cifuentes and Marcelo Baeza

    Microbial Cell Factories 2017 16:75

    Published on: 2 May 2017

  11. Research

    Direct expression of active human tissue inhibitors of metalloproteinases by periplasmic secretion in Escherichia coli

    As regulators of multifunctional metalloproteinases including MMP, ADAM and ADAMTS families, tissue inhibitors of metalloproteinases (TIMPs) play a pivotal role in extracellular matrix remodeling, which is inv...

    Ki Baek Lee, Dong Hyun Nam, Jacob A. M. Nuhn, Juan Wang, Ian C. Schneider and Xin Ge

    Microbial Cell Factories 2017 16:73

    Published on: 28 April 2017

  12. Research

    Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

    Recombinant expression of toxic proteins remains a challenging problem. One potential method to shield toxicity and thus improve expression of these proteins is to encapsulate them within protein compartments ...

    Mimi C. Yung, Feliza A. Bourguet, Timothy S. Carpenter and Matthew A. Coleman

    Microbial Cell Factories 2017 16:71

    Published on: 26 April 2017

  13. Research

    Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

    The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by...

    Francesca Zerbini, Ilaria Zanella, Davide Fraccascia, Enrico König, Carmela Irene, Luca F. Frattini, Michele Tomasi, Laura Fantappiè, Luisa Ganfini, Elena Caproni, Matteo Parri, Alberto Grandi and Guido Grandi

    Microbial Cell Factories 2017 16:68

    Published on: 24 April 2017

  14. Research

    A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

    Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a centr...

    Dongbo Cai, Hao Wang, Penghui He, Chengjun Zhu, Qin Wang, Xuetuan Wei, Christopher T. Nomura and Shouwen Chen

    Microbial Cell Factories 2017 16:70

    Published on: 24 April 2017

  15. Research

    Inheritance of brewing-relevant phenotypes in constructed Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids

    Interspecific hybridization has proven to be a potentially valuable technique for generating de novo lager yeast strains that possess diverse and improved traits compared to their parent strains. To further en...

    Kristoffer Krogerus, Tuulikki Seppänen-Laakso, Sandra Castillo and Brian Gibson

    Microbial Cell Factories 2017 16:66

    Published on: 21 April 2017

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