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Research
Recently, the discovery of the elansolids, a group of macrolides, was reported. The molecules show activity against methicillin-resistant Staphylococcus aureus as well as other gram-positive organisms. This fact ...
Microbial Cell Factories 2017 16:143
Published on: 17 August 2017
Research
In recent years the production of biobased biodegradable plastics has been of interest of researchers partly due to the accumulation of non-biodegradable plastics in the environment and to the opportunity for ...
Microbial Cell Factories 2017 16:144
Published on: 17 August 2017
Research
An inducible promoter for recombinant protein expression provides substantial benefits because under induction conditions cellular energy and metabolic capability can be directed into protein synthesis. The mo...
Microbial Cell Factories 2017 16:145
Published on: 17 August 2017
Research
The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is ...
Microbial Cell Factories 2017 16:142
Published on: 16 August 2017
Research
The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed bas...
Microbial Cell Factories 2017 16:141
Published on: 15 August 2017
Research
There are an increasing number of studies regarding genetic manipulation of cyanobacteria to produce commercially interesting compounds. The majority of these works study the expression and optimization of a s...
Microbial Cell Factories 2017 16:140
Published on: 15 August 2017
Erratum
Microbial Cell Factories 2017 16:139
Published on: 9 August 2017
The original article was published in Microbial Cell Factories 2017 16:55
Research
Antifreeze proteins (AFPs) production is a survival strategy of psychrophiles in ice. These proteins have potential in frozen food industry avoiding the damage in the structure of animal or vegetal foods. More...
Microbial Cell Factories 2017 16:138
Published on: 7 August 2017
Review
Rhamnolipids are a class of biosurfactants which contain rhamnose as the sugar moiety linked to β-hydroxylated fatty acid chains. Rhamnolipids can be widely applied in many industries including petroleum, food...
Microbial Cell Factories 2017 16:137
Published on: 5 August 2017
Research
Chalcones are the biogenetic precursors of all known flavonoids, which play an essential role in various metabolic processes in photosynthesizing organisms. The use of whole cyanobacteria cells in a two-step, ...
Microbial Cell Factories 2017 16:136
Published on: 4 August 2017
Technical Notes
Plasmids are widely used and essential tools in molecular biology. However, plasmids often impose a metabolic burden and are only temporarily useful for genetic engineering, bio-sensing and characterization pu...
Microbial Cell Factories 2017 16:135
Published on: 2 August 2017
Technical Notes
Cell disruption is a key unit operation to make valuable, intracellular target products accessible for further downstream unit operations. Independent of the applied cell disruption method, each cell disruptio...
Microbial Cell Factories 2017 16:134
Published on: 1 August 2017
Research
Transaminases have become a key tool in biocatalysis to introduce the amine functionality into a range of molecules like prochiral α-ketoacids and ketones. However, due to the necessity of shifting the equilib...
Microbial Cell Factories 2017 16:132
Published on: 28 July 2017
Research
A cellular stress response is triggered upon induction of recombinant protein expression which feedback inhibits both growth as well as protein synthesis. In order to separate these two effects, it was decided...
Microbial Cell Factories 2017 16:133
Published on: 28 July 2017
Research
The ideal protein expression system should provide recombinant proteins in high quality and quantity involving low production costs only. However, especially for complex therapeutic proteins like monoclonal an...
Microbial Cell Factories 2017 16:131
Published on: 27 July 2017
Research
Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. ...
Microbial Cell Factories 2017 16:130
Published on: 25 July 2017
Research
Filamentous fungi are commonly used as production hosts for bulk enzymes in biotechnological applications. Their robust and quick growth combined with their ability to secrete large amounts of protein directly...
Microbial Cell Factories 2017 16:128
Published on: 25 July 2017
Research
Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting bioma...
Microbial Cell Factories 2017 16:129
Published on: 25 July 2017
Research
Microbial reductive dechlorination of polychlorinated biphenyls (PCBs) plays a major role in detoxifying anoxic contaminated freshwater and marine sediments from PCBs. Known members of the phylum Chloroflexi are ...
Microbial Cell Factories 2017 16:127
Published on: 24 July 2017
Research
Lipomyces starkeyi is one of the leading lipid-producing microorganisms reported to date; its genetic transformation was only recently reported. Our aim is to engineer L. starkeyi to s...
Microbial Cell Factories 2017 16:126
Published on: 24 July 2017
Review
Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield ...
Microbial Cell Factories 2017 16:124
Published on: 19 July 2017
Review
Plant natural products (PNPs) are widely used as pharmaceuticals, nutraceuticals, seasonings, pigments, etc., with a huge commercial value on the global market. However, most of these PNPs are still being extr...
Microbial Cell Factories 2017 16:125
Published on: 19 July 2017
Research
Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enz...
Microbial Cell Factories 2017 16:123
Published on: 18 July 2017
Research
Propionic acid is used primarily as a food preservative with smaller applications as a chemical building block for the production of many products including fabrics, cosmetics, drugs, and plastics. Biological ...
Microbial Cell Factories 2017 16:121
Published on: 17 July 2017
Research
The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and p...
Microbial Cell Factories 2017 16:122
Published on: 17 July 2017
