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  1. Research

    Optimization of the biotechnological production of a novel class of anti-MRSA antibiotics from Chitinophaga sancti

    Recently, the discovery of the elansolids, a group of macrolides, was reported. The molecules show activity against methicillin-resistant Staphylococcus aureus as well as other gram-positive organisms. This fact ...

    Amelie Beckmann, Stephan Hüttel, Viktoria Schmitt, Rolf Müller and Marc Stadler

    Microbial Cell Factories 2017 16:143

    Published on: 17 August 2017

  2. Research

    Enhancement of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation in Arxula adeninivorans by stabilization of production

    In recent years the production of biobased biodegradable plastics has been of interest of researchers partly due to the accumulation of non-biodegradable plastics in the environment and to the opportunity for ...

    Mateusz Biernacki, Marek Marzec, Thomas Roick, Reinhard Pätz, Kim Baronian, Rüdiger Bode and Gotthard Kunze

    Microbial Cell Factories 2017 16:144

    Published on: 17 August 2017

  3. Research

    Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica

    An inducible promoter for recombinant protein expression provides substantial benefits because under induction conditions cellular energy and metabolic capability can be directed into protein synthesis. The mo...

    Roshan P. Shrestha and Mark Hildebrand

    Microbial Cell Factories 2017 16:145

    Published on: 17 August 2017

  4. Research

    Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus

    The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is ...

    Ying-jie Yang, Ye Wang, Zhi-feng Li, Ya Gong, Peng Zhang, Wen-chao Hu, Duo-hong Sheng and Yue-zhong Li

    Microbial Cell Factories 2017 16:142

    Published on: 16 August 2017

  5. Research

    New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

    The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed bas...

    Marion Trassaert, Marie Vandermies, Fréderic Carly, Olivia Denies, Stéphane Thomas, Patrick Fickers and Jean-Marc Nicaud

    Microbial Cell Factories 2017 16:141

    Published on: 15 August 2017

  6. Research

    Responses of Synechocystis sp. PCC 6803 to heterologous biosynthetic pathways

    There are an increasing number of studies regarding genetic manipulation of cyanobacteria to produce commercially interesting compounds. The majority of these works study the expression and optimization of a s...

    Konstantinos Vavitsas, Emil Østergaard Rue, Lára Kristín Stefánsdóttir, Thiyagarajan Gnanasekaran, Andreas Blennow, Christoph Crocoll, Steinn Gudmundsson and Poul Erik Jensen

    Microbial Cell Factories 2017 16:140

    Published on: 15 August 2017

  7. Research

    Structure and application of antifreeze proteins from Antarctic bacteria

    Antifreeze proteins (AFPs) production is a survival strategy of psychrophiles in ice. These proteins have potential in frozen food industry avoiding the damage in the structure of animal or vegetal foods. More...

    Patricio A. Muñoz, Sebastián L. Márquez, Fernando D. González-Nilo, Valeria Márquez-Miranda and Jenny M. Blamey

    Microbial Cell Factories 2017 16:138

    Published on: 7 August 2017

  8. Technical Notes

    A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

    Plasmids are widely used and essential tools in molecular biology. However, plasmids often impose a metabolic burden and are only temporarily useful for genetic engineering, bio-sensing and characterization pu...

    Ida Lauritsen, Andreas Porse, Morten O. A. Sommer and Morten H. H. Nørholm

    Microbial Cell Factories 2017 16:135

    Published on: 2 August 2017

  9. Technical Notes

    A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

    Cell disruption is a key unit operation to make valuable, intracellular target products accessible for further downstream unit operations. Independent of the applied cell disruption method, each cell disruptio...

    Britta Eggenreich, Vignesh Rajamanickam, David Johannes Wurm, Jens Fricke, Christoph Herwig and Oliver Spadiut

    Microbial Cell Factories 2017 16:134

    Published on: 1 August 2017

  10. Research

    In vivo plug-and-play: a modular multi-enzyme single-cell catalyst for the asymmetric amination of ketoacids and ketones

    Transaminases have become a key tool in biocatalysis to introduce the amine functionality into a range of molecules like prochiral α-ketoacids and ketones. However, due to the necessity of shifting the equilib...

    Judith E. Farnberger, Elisabeth Lorenz, Nina Richter, Volker F. Wendisch and Wolfgang Kroutil

    Microbial Cell Factories 2017 16:132

    Published on: 28 July 2017

  11. Research

    Identifying genomic targets for protein over-expression by “omics” analysis of Quiescent Escherichia coli cultures

    A cellular stress response is triggered upon induction of recombinant protein expression which feedback inhibits both growth as well as protein synthesis. In order to separate these two effects, it was decided...

    Shubhashree Mahalik, Ashish Kumar Sharma, Priyanka Jain and Krishna Jyoti Mukherjee

    Microbial Cell Factories 2017 16:133

    Published on: 28 July 2017

  12. Research

    From hybridomas to a robust microalgal-based production platform: molecular design of a diatom secreting monoclonal antibodies directed against the Marburg virus nucleoprotein

    The ideal protein expression system should provide recombinant proteins in high quality and quantity involving low production costs only. However, especially for complex therapeutic proteins like monoclonal an...

    Franziska Hempel, Michael Maurer, Björn Brockmann, Christian Mayer, Nadine Biedenkopf, Anne Kelterbaum, Stephan Becker and Uwe G. Maier

    Microbial Cell Factories 2017 16:131

    Published on: 27 July 2017

  13. Research

    Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis

    Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. ...

    Dinh Thi Minh Tran, Trang Thi Phuong Phan, Thanh Kieu Huynh, Ngan Thi Kim Dang, Phuong Thi Kim Huynh, Tri Minh Nguyen, Tuom Thi Tinh Truong, Thuoc Linh Tran, Wolfgang Schumann and Hoang Duc Nguyen

    Microbial Cell Factories 2017 16:130

    Published on: 25 July 2017

  14. Research

    Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

    Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting bioma...

    Norma A. Valdez-Cruz, Greta I. Reynoso-Cereceda, Saumel Pérez-Rodriguez, Sara Restrepo-Pineda, Jesus González-Santana, Alejandro Olvera, Guadalupe Zavala, Alejandro Alagón and Mauricio A. Trujillo-Roldán

    Microbial Cell Factories 2017 16:129

    Published on: 25 July 2017

  15. Research

    Identification of two organohalide-respiring Dehalococcoidia associated to different dechlorination activities in PCB-impacted marine sediments

    Microbial reductive dechlorination of polychlorinated biphenyls (PCBs) plays a major role in detoxifying anoxic contaminated freshwater and marine sediments from PCBs. Known members of the phylum Chloroflexi are ...

    Andrea Nuzzo, Andrea Negroni, Giulio Zanaroli and Fabio Fava

    Microbial Cell Factories 2017 16:127

    Published on: 24 July 2017

  16. Research

    Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi

    Lipomyces starkeyi is one of the leading lipid-producing microorganisms reported to date; its genetic transformation was only recently reported. Our aim is to engineer L. starkeyi to s...

    Qi Xu, Eric P. Knoshaug, Wei Wang, Markus Alahuhta, John O. Baker, Shihui Yang, Todd Vander Wall, Stephen R. Decker, Michael E. Himmel, Min Zhang and Hui Wei

    Microbial Cell Factories 2017 16:126

    Published on: 24 July 2017

  17. Review

    Bottleneck in secretion of α-amylase in Bacillus subtilis

    Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield ...

    Shaomin Yan and Guang Wu

    Microbial Cell Factories 2017 16:124

    Published on: 19 July 2017

  18. Research

    Awakening sleeping beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase

    Propionic acid is used primarily as a food preservative with smaller applications as a chemical building block for the production of many products including fabrics, cosmetics, drugs, and plastics. Biological ...

    Ricardo Axayacatl Gonzalez-Garcia, Tim McCubbin, Annalena Wille, Manuel Plan, Lars Keld Nielsen and Esteban Marcellin

    Microbial Cell Factories 2017 16:121

    Published on: 17 July 2017

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