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  1. Content type: Review


    Authors: Nabih A Baeshen, Mohammed N Baeshen, Abdullah Sheikh, Roop S Bora, Mohamed Morsi M Ahmed, Hassan A I Ramadan, Kulvinder Singh Saini and Elrashdy M Redwan

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Thematic series
Recombinant antibody technology: from phage display panning to complex combined approaches
Edited by Ario de Marco
Collection published: 22nd January 2018


Thematic series
Engineering cell factories for production of bulk and fine chemicals: from designing principles to industrial-scale production
Edited by Yin Li
Collection published: 24 June 2017


Thematic series
CRISPR/Cas9 and the microbial cell factory
Edited by George Guo-Qiang Chen
Collection published: 16 February 2017


Thematic series
Microbial food biotechnology
Edited by: Prof Maria Papagianni
Collection published: 3 May 2016


Thematic series
Systems biotechnology and metabolic engineering
Edited by: Prof Sang Yup Lee
Collection published: 19 June 2014

Thematic series
Added value chemicals and biofuels
Edited by: Prof Akihiko Kondo
Collection published: 9 June 2014

Thematic series
Yeast biotechnology
Microbial Cell Factories
Edited by: Prof Diethard Mattanovich
Collection published: 6 May 2014

Thematic series
Recombinant protein quality: approaches for the quantitative evaluation of functional and structural features of purified proteins and antibodies
Edited by: Prof Ario de Marco
Collection published: 11 April 2014

Cross journal collection
Reviewer acknowledgements 2013
Collection published: 26 February 2014

Recombinant antibody technology: from phage display panning to complex combined approaches

Recombinant antibody technology is considered a fast and inexpensive method to recover new binders. It is also acknowledged that it enables to expand the repertoire represented by natural binders issued from immunization.  This improvement can be realized by forcing the system at different levels: i) design of the library in terms of dimension and structural characteristics; ii) panning and screening conditions for selecting antibodies with specific biophysical features; iii) in vitro and in silico maturation procedures paired to molecular engineering for optimizing the binders in the function of their final applications.

In many cases, the advancement in this research field has been the consequence of applying in creative form combinations of methodologies which initially developed separately. Examples are the alternate use of phage and yeast/ribosomal/bacterial display, the introduction of rational mutagenesis based on computational modeling to optimize the initial hits recovered by conventional panning, the use of automated systems that allow the analysis of huge amounts of biochemical (from ELISA, flow-cytometry, affinity measurement) and NGS data. These approaches simplified the isolation of antibodies for difficult targets (membrane proteins, toxic and non-immunogenic targets), specific applications (structural chaperones, allosteric inhibitors, detectors of structural conformation), and better biophysical characteristics (higher stability and yields, stronger and more selective binding, lower immunogenicity).

Edited by Ario de Marco, University of Nova Gorica, Slovenia. If you are interested in contributing to the Special Issue, please contact the Editor:

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Aims and scope

Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology.

The journal is divided into the following editorial sections:

Metabolic engineering
Synthetic biology
Whole-cell biocatalysis
Microbial regulations
Recombinant protein production/bioprocessing
Production of natural compounds
Systems biology of cell factories
Microbial production processes
Cell-free systems

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