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  1. In Saccharomyces cerevisiae galactose is initially metabolized through the Leloir pathway after which glucose 6-phosphate enters glycolysis. Galactose is controlled both by glucose repression and by galactose ind...

    Authors: Rosa Garcia Sanchez, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund
    Citation: Microbial Cell Factories 2010 9:40
  2. Aeromonas hydrophila is a serious pathogen and can cause hemorrhagic septicemia in fish. To control this disease, antibiotics and chemicals are widely used which can consequently result in "superbugs" and chemica...

    Authors: Ruidong Chen, Zhigang Zhou, Yanan Cao, Yingguo Bai and Bin Yao
    Citation: Microbial Cell Factories 2010 9:39
  3. Evolvability is an intrinsic feature of all living cells. However, newly emerging, evolved features can be undesirable when genetic circuits, designed and fabricated by rational, synthetic biological approache...

    Authors: Kinga Umenhoffer, Tamás Fehér, Gabriella Balikó, Ferhan Ayaydin, János Pósfai, Frederick R Blattner and György Pósfai
    Citation: Microbial Cell Factories 2010 9:38
  4. Staphylococcal (or micrococcal) nuclease or thermonuclease (SNase or Nuc) is a naturally-secreted nucleic acid degrading enzyme that participates in Staphylococcus aureus spread in the infected host. Purified Nuc...

    Authors: Nicolas Trémillon, Nicolas Issaly, Julien Mozo, Thomas Duvignau, Hervé Ginisty, Eric Devic and Isabelle Poquet
    Citation: Microbial Cell Factories 2010 9:37
  5. Glycerol is the major by-product accounting for up to 5% of the carbon in Saccharomyces cerevisiae ethanolic fermentation. Decreasing glycerol formation may redirect part of the carbon toward ethanol production. ...

    Authors: Julien Pagliardini, Georg Hubmann, Carine Bideaux, Sandrine Alfenore, Elke Nevoigt and Stéphane E Guillouet
    Citation: Microbial Cell Factories 2010 9:36
  6. Bioprocess development of recombinant proteins is time consuming and laborious as many factors influence the accumulation of the product in the soluble and active form. Currently, in most cases the development...

    Authors: Juozas Šiurkus, Johanna Panula-Perälä, Uwe Horn, Mario Kraft, Renata Rimšeliene and Peter Neubauer
    Citation: Microbial Cell Factories 2010 9:35
  7. Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among b...

    Authors: Anna Zanfardino, Odile F Restaino, Eugenio Notomista, Donatella Cimini, Chiara Schiraldi, Mario De Rosa, Maurilio De Felice and Mario Varcamonti
    Citation: Microbial Cell Factories 2010 9:34
  8. Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a ...

    Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo and Bin Yao
    Citation: Microbial Cell Factories 2010 9:33
  9. The filamentous fungus T. reesei effectively degrades cellulose and is known to produce various cellulolytic enzymes such as β-glucosidase, endoglucanase, and cellobiohydrolase. The expression levels of each cell...

    Authors: Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda and Akihiko Kondo
    Citation: Microbial Cell Factories 2010 9:32
  10. The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increa...

    Authors: Chandrasekhar Gurramkonda, Sulena Polez, Natasa Skoko, Ahmad Adnan, Thomas Gäbel, Dipti Chugh, Sathyamangalam Swaminathan, Navin Khanna, Sergio Tisminetzky and Ursula Rinas
    Citation: Microbial Cell Factories 2010 9:31
  11. The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the ...

    Authors: Sampali Banerjee, Jitendra Kumar, Anjali Apte-Deshpande and Sriram Padmanabhan
    Citation: Microbial Cell Factories 2010 9:30
  12. Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, howe...

    Authors: Hakan Dortay and Bernd Mueller-Roeber
    Citation: Microbial Cell Factories 2010 9:29
  13. PhaR which is a repressor protein for microbial polyhydroxyalkanoates (PHA) biosynthesis, is able to attach to bacterial PHA granules in vivo, was developed as an affinity tag for in vitro protein purification. F...

    Authors: Shuang Zhang, Zhi Hui Wang and Guo Qiang Chen
    Citation: Microbial Cell Factories 2010 9:28
  14. Recombinant gene expression is among the most important techniques used both in molecular and medical research and in industrial settings. Today, two recombinant expression systems are particularly well repres...

    Authors: Hans Peter Sørensen
    Citation: Microbial Cell Factories 2010 9:27
  15. The green fluorescent protein has revolutionized many areas of cell biology and biotechnology since it is widely used in determining gene expression and for localization of protein expression. Expression of re...

    Authors: Shardul S Salunkhe, Veena A Raiker, Sachin Rewanwar, Prakash Kotwal, Avijeet Kumar and Sriram Padmanabhan
    Citation: Microbial Cell Factories 2010 9:26
  16. Trigonopsis variabilis D-amino acid oxidase (Tv DAO) is a well characterized enzyme used for cephalosporin C conversion on industrial scale. However, the demands on the enzyme with respect to activity, operationa...

    Authors: Sandra Abad, Jozef Nahalka, Gabriele Bergler, S Alison Arnold, Robert Speight, Ian Fotheringham, Bernd Nidetzky and Anton Glieder
    Citation: Microbial Cell Factories 2010 9:24
  17. The filamentous fungus Aspergillus niger is well-known as a producer of primary metabolites and extracellular proteins. For example, glucoamylase is the most efficiently secreted protein of Aspergillus niger, thu...

    Authors: Xin Lu, Jibin Sun, Manfred Nimtz, Josef Wissing, An-Ping Zeng and Ursula Rinas
    Citation: Microbial Cell Factories 2010 9:23
  18. The overexpression of scFv antibody fragments in the periplasmic space of Escherichia coli frequently results in extensive protein misfolding and loss of cell viability. Although protein folding factors such as S...

    Authors: Dave Siak-Wei Ow, Denis Yong-Xiang Lim, Peter Morin Nissom, Andrea Camattari and Victor Vai-Tak Wong
    Citation: Microbial Cell Factories 2010 9:22
  19. Shikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed to produce SA in Escherichia coli strains derived fro...

    Authors: Adelfo Escalante, Rocío Calderón, Araceli Valdivia, Ramón de Anda, Georgina Hernández, Octavio T Ramírez, Guillermo Gosset and Francisco Bolívar
    Citation: Microbial Cell Factories 2010 9:21
  20. Mannans are one of the key polymers in hemicellulose, a major component of lignocellulose. The Mannan endo-1,4-β-mannosidase or 1,4-β-D-mannanase (EC 3.2.1.78), commonly named β-mannanase, is an enzyme that can c...

    Authors: Chomphunuch Songsiriritthigul, Bancha Buranabanyat, Dietmar Haltrich and Montarop Yamabhai
    Citation: Microbial Cell Factories 2010 9:20
  21. Protein over-production in Escherichia coli often results in formation of inclusion bodies (IBs). Some recent reports have shown that the aggregation into IBs does not necessarily mean that the target protein is ...

    Authors: Ermenegilda Parrilli, Maria Giuliani, Gennaro Marino and Maria Luisa Tutino
    Citation: Microbial Cell Factories 2010 9:19
  22. The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use to produce recombinant proteins in prokariotic ce...

    Authors: Norma A Valdez-Cruz, Luis Caspeta, Néstor O Pérez, Octavio T Ramírez and Mauricio A Trujillo-Roldán
    Citation: Microbial Cell Factories 2010 9:18
  23. In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-through...

    Authors: Eva García-Ruiz, Diana Maté, Antonio Ballesteros, Angel T Martinez and Miguel Alcalde
    Citation: Microbial Cell Factories 2010 9:17
  24. In spite of the substantial metabolic engineering effort previously devoted to the development of Saccharomyces cerevisiae strains capable of fermenting both the hexose and pentose sugars present in lignocellulos...

    Authors: Stefan Krahulec, Barbara Petschacher, Michael Wallner, Karin Longus, Mario Klimacek and Bernd Nidetzky
    Citation: Microbial Cell Factories 2010 9:16
  25. Since about three decades, Saccharomyces cerevisiae can be engineered to efficiently produce proteins and metabolites. Even recognizing that in baker's yeast one determining step for the glucose consumption rate ...

    Authors: Giorgia Rossi, Michael Sauer, Danilo Porro and Paola Branduardi
    Citation: Microbial Cell Factories 2010 9:15
  26. The heterologous production of the industrially relevant fungal enzyme pyranose 2-oxidase in the prokaryotic host E. coli was investigated using 3 different expression systems, i.e. the well-studied T7 RNA polyme...

    Authors: Oliver Spadiut, Gerald Posch, Roland Ludwig, Dietmar Haltrich and Clemens K Peterbauer
    Citation: Microbial Cell Factories 2010 9:14
  27. Bacteria possess a reservoir of metabolic functionalities ready to be exploited for multiple purposes. The use of microorganisms to clean up xenobiotics from polluted ecosystems (e.g. soil and water) represent...

    Authors: Massimo Marzorati, Annalisa Balloi, Francesca de Ferra, Lorenzo Corallo, Giovanna Carpani, Lieven Wittebolle, Willy Verstraete and Daniele Daffonchio
    Citation: Microbial Cell Factories 2010 9:12
  28. Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks of...

    Authors: Mirja Krause, Kaisa Ukkonen, Tatu Haataja, Maria Ruottinen, Tuomo Glumoff, Antje Neubauer, Peter Neubauer and Antti Vasala
    Citation: Microbial Cell Factories 2010 9:11
  29. The bioremediation of soils impacted by diesel fuels is very often limited by the lack of indigenous microflora with the required broad substrate specificity. In such cases, the soil inoculation with cultures ...

    Authors: Giulio Zanaroli, Sara Di Toro, Daniela Todaro, Giovanna C Varese, Antonio Bertolotto and Fabio Fava
    Citation: Microbial Cell Factories 2010 9:10
  30. Wine Saccharomyces cerevisiae strains, adapted to anaerobic must fermentations, suffer oxidative stress when they are grown under aerobic conditions for biomass propagation in the industrial process of active dry...

    Authors: Rocío Gómez-Pastor, Roberto Pérez-Torrado, Elisa Cabiscol, Joaquim Ros and Emilia Matallana
    Citation: Microbial Cell Factories 2010 9:9

    The Erratum to this article has been published in Microbial Cell Factories 2012 11:31

  31. It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of prac...

    Authors: Rahul Kumar and Kazuyuki Shimizu
    Citation: Microbial Cell Factories 2010 9:8
  32. Zygosaccharomyces bailii is a diploid budding yeast still poorly characterized, but widely recognised as tolerant to several stresses, most of which related to industrial processes of production. Because of that,...

    Authors: Simone Passolunghi, Luca Riboldi, Laura Dato, Danilo Porro and Paola Branduardi
    Citation: Microbial Cell Factories 2010 9:7
  33. Nikkomycins are a group of peptidyl nucleoside antibiotics produced by Streptomyces ansochromogenes. They are competitive inhibitors of chitin synthase and show potent fungicidal, insecticidal, and acaricidal act...

    Authors: Guojian Liao, Jine Li, Lei Li, Haihua Yang, Yuqing Tian and Huarong Tan
    Citation: Microbial Cell Factories 2010 9:6
  34. Chitosanases (EC 3.2.1.132) hydrolyze the polysaccharide chitosan, which is composed of partially acetylated β-(1,4)-linked glucosamine residues. In nature, chitosanases are produced by a number of Gram-positi...

    Authors: Mads G Johnsen, Ole C Hansen and Peter Stougaard
    Citation: Microbial Cell Factories 2010 9:5
  35. In spite of its advantageous physiological properties for bioprocess applications, the use of the yeast Kluyveromyces marxianus as a host for heterologous protein production has been very limited, in constrast to...

    Authors: Saul N Rocha, José Abrahão-Neto, María E Cerdán, María I González-Siso and Andreas K Gombert
    Citation: Microbial Cell Factories 2010 9:4
  36. The bacterial endospore (spore) has recently been proposed as a new surface display system. Antigens and enzymes have been successfully exposed on the surface layers of the Bacillus subtilis spore, but only in a ...

    Authors: Krzysztof Hinc, Rachele Isticato, Marcin Dembek, Joanna Karczewska, Adam Iwanicki, Grażyna Peszyńska-Sularz, Maurilio De Felice, Michał Obuchowski and Ezio Ricca
    Citation: Microbial Cell Factories 2010 9:2
  37. Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and secretion of human interleukin-1β (IL1β), using the cell wall protein Pir4 as f...

    Authors: Lucia Paciello, Elisabetta de Alteriis, Cristina Mazzoni, Vanessa Palermo, Jesus Zueco and Palma Parascandola
    Citation: Microbial Cell Factories 2009 8:70
  38. The last four years have brought exciting progress in membrane protein research. Finally those many efforts that have been put into expression of eukaryotic membrane proteins are coming to fruition and enable ...

    Authors: Maria Freigassner, Harald Pichler and Anton Glieder
    Citation: Microbial Cell Factories 2009 8:69
  39. In the past decade, an enormous number of new bioprocesses have evolved in the biotechnology industry. These bioprocesses have to be developed fast and at a maximum productivity. Up to now, only few microbiore...

    Authors: Frank Kensy, Christoph Engelbrecht and Jochen Büchs
    Citation: Microbial Cell Factories 2009 8:68
  40. Xylose is a second most abundant sugar component of lignocellulose besides glucose. Efficient fermentation of xylose is important for the economics of biomass-based biorefineries. However, sugar mixtures are s...

    Authors: Chuan Ren, Tingjian Chen, Jingqing Zhang, Ling Liang and Zhanglin Lin
    Citation: Microbial Cell Factories 2009 8:66
  41. Analysis of the cell operation at the metabolic level requires collecting data of different types and to determine their confidence level. In addition, the acquired information has to be combined in order to o...

    Authors: Marc Carnicer, Kristin Baumann, Isabelle Töplitz, Francesc Sánchez-Ferrando, Diethard Mattanovich, Pau Ferrer and Joan Albiol
    Citation: Microbial Cell Factories 2009 8:65
  42. Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors an...

    Authors: Karl Rumbold, Hugo JJ van Buijsen, Karin M Overkamp, Johan W van Groenestijn, Peter J Punt and Mariët J van der Werf
    Citation: Microbial Cell Factories 2009 8:64
  43. Phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. To face these drawbacks, producers have become increasingly dependent on agrochemicals...

    Authors: Anthony Arguelles-Arias, Marc Ongena, Badre Halimi, Yannick Lara, Alain Brans, Bernard Joris and Patrick Fickers
    Citation: Microbial Cell Factories 2009 8:63
  44. The 190-kDa Paenibacillus β-1,3-glucanase (LamA) contains a catalytic module of the glycoside hydrolase family 16 (GH16) and several auxiliary domains. Of these, a discoidin domain (DS domain), present in both eu...

    Authors: Yueh-Mei Cheng, Feng-Chia Hsieh and Menghsiao Meng
    Citation: Microbial Cell Factories 2009 8:62
  45. Nikkomycins are a group of peptidyl nucleoside antibiotics and act as potent inhibitors of chitin synthases in fungi and insects. Nikkomycin X and Z are the main components produced by Streptomyces ansochromogene...

    Authors: Guojian Liao, Jine Li, Lei Li, Haihua Yang, Yuqing Tian and Huarong Tan
    Citation: Microbial Cell Factories 2009 8:61

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