- Open Access
Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli
© Tseng et al; licensee BioMed Central Ltd. 2010
- Received: 28 September 2010
- Accepted: 27 November 2010
- Published: 27 November 2010
The ability to synthesize chiral building block molecules with high optical purity is of considerable importance to the fine chemical and pharmaceutical industries. Production of one such compound, 3-hydroxyvalerate (3HV), has previously been studied with respect to the in vivo or in vitro enzymatic depolymerization of biologically-derived co-polymers of poly(3-hydroxybutyrate-co-3-hydroxyvalerate). However, production of this biopolymeric precursor typically necessitates the supplementation of a secondary carbon source (e.g., propionate) into the culture medium. In addition, previous approaches for producing 3HV have not focused on its enantiopure synthesis, and thus suffer from increased costs for product purification.
Here, we report the selective biosynthesis of each 3HV stereoisomer from a single, renewable carbon source using synthetic metabolic pathways in recombinant strains of Escherichia coli. The product chirality was controlled by utilizing two reductases of opposing stereoselectivity. Improvement of the biosynthetic pathway activity and host background was carried out to elevate both the 3HV titers and 3HV/3HB ratios. Overall, shake-flask titers as high as 0.31 g/L and 0.50 g/L of (S)-3HV and (R)-3HV, respectively, were achieved in glucose-fed cultures, whereas glycerol-fed cultures yielded up to 0.19 g/L and 0.96 g/L of (S)-3HV and (R)-3HV, respectively.
Our work represents the first report of direct microbial production of enantiomerically pure 3HV from a single carbon source. Continued engineering of host strains and pathway enzymes will ultimately lead to more economical production of chiral 3HV.
- Levulinic Acid
- Multiple Cloning Site
- Single Carbon Source
- Threonine Deaminase
The efficient production of enantiomerically pure chemicals from renewable resources has gained considerable attention especially in the fine chemical/pharmaceutical industry. Stereo-selective chemical processes generally employ expensive chiral catalysts, require harsh physical conditions and solvents, and suffer from extensive byproduct formation. In contrast, enzyme-catalyzed reactions are highly stereo-selective and can be performed in aqueous solutions under mild conditions . As a result, replacing chemical processes by biological ones for the synthesis of chiral compounds has been extensively investigated not only due to superior stereo-selectivity of enzymatic reactions but also due to sustainability as an implementation of green chemistry [2–5]. One example is the production of hydroxyacids, a family of versatile chiral molecules containing one hydroxyl group and one carboxyl group . These molecules have the potential to serve as useful chiral building blocks for a diverse range of products, including polyhydroxyalkanoates (PHAs) (biodegradable polymers) and optically-active fine chemicals, such as pharmaceuticals, vitamins, antibiotics, and flavor compounds [7–10]. Naturally, hydroxyacids are primarily found to be polymerized as PHAs where they serve as intracellular storage materials for numerous microbes. Those PHAs consist mostly of monomers with 3-hydroxy, 4-hydroxy, and 5-hydroxy groups with different lengths of main and side chains .
A novel pathway for the production of PHBV solely from glycerol has been established in recombinant Salmonella enterica Serovar Typhimurium, containing a heterologous pathway that converts succinyl-CoA to propionyl-CoA, the essential precursor molecule of 3HV-CoA in PHBV synthesis . However, expensive cyanocobalamin (CN-B12) was supplemented to the medium to provide the precursor of coenzyme B12 required for the activity of one of the enzymes in the B12-dependent biosynthetic pathway. It should also be noted that the pathway only functioned in S. enterica, a pathogen, but not E. coli, thus limiting its applicability to other industrially-relevant host organisms. In this study, we proposed an alternative biosynthetic pathway that does not require coenzyme B12 for its functionality to synthesize 3HV from glucose or glycerol. Specifically, we metabolically engineered E. coli to exploit its native metabolism for endogenous supply of propionyl-CoA via the threonine biosynthesis pathway, and introduced a heterologous pathway for chiral 3HV biosynthesis using acetyl-CoA and propionyl-CoA as precursor molecules. As stated above, several previous methods for producing 3HV did not focus on enantiopure synthesis. Similarly, due to the stereospecific constraints of PHBV synthesis, in which polymers are composed exclusively of (R)-3HB and (R)-3HV monomer units, the synthesis of (S)-3HV from PHBV remains effectively impossible. On the contrary, our proposed pathway makes possible the direct synthesis of both enantiomerically pure (R)-3HV and (S)-3HV.
We have identified a pathway which combines elements of our previously developed chiral 3HB biosynthesis pathway together with the natural threonine biosynthesis pathway of E. coli for direct biosynthesis of chiral 3HV (Figure 1). In the proposed pathway, chiral 3HV is produced from direct hydrolysis of 3HV-CoA catalyzed by a thioesterase II (encoded by tesB) where 3HV-CoA is obtained from condensation of one acetyl-CoA and one propionyl-CoA to form 3-ketovaleryl-CoA catalyzed by a thiolase (encoded by bktB), followed by a reduction of the 3-ketovaleryl-CoA to 3HV-CoA catalyzed by a 3-hydroxybutyryl-CoA dehydrogenase. Here, two enantio-selective 3-hydroxybutyryl-CoA dehydrogenases were utilized to control the chirality of 3HV-CoA produced. The NADPH-dependent dehydrogenase encoded by phaB produces (R)-3HV-CoA while the NADH-dependent dehydrogenase encoded by hbd produces (S)-3HV-CoA. It should be noted that in order to yield the highest 3HV titers and 3HV/3HB ratios, BktB was used as the thiolase in this study as opposed to other thiolases such as PhaA from R. eutropha H16 or Thil from C. acetobutylicum ATCC 824 because BktB has been shown to have highest in vitro enzyme activity towards the C5 substrate while PhaA and Thil were specific towards the C4 substrate . Next, a pathway allowing for endogenous propionyl-CoA synthesis from glucose or glycerol, through the threonine metabolic pathway intermediate 2-ketobutyrate, was introduced to circumvent the need for feeding propionate. To examine the upstream pathway for endogenous supply of propionyl-CoA, we used a bottom-up approach where 2-ketobutyrate and threonine were, at first, fed to provide propionyl-CoA, in addition to glucose, to support 3HV production. In the final stage, a single carbon source of glucose or glycerol was used to provide both acetyl-CoA and propionyl-CoA to support 3HV biosynthesis in our metabolically engineered E. coli.
Overall, in this study we successfully demonstrated the direct biological production of enantiomerically pure (R)-3HV and (S)-3HV from a single carbon source. Improvements of the biosynthetic pathway and E. coli host strains have also been carried out to elevate 3HV titers and 3HV/3HB ratios.
3HV Synthesis from Glucose and propionate
Acetyl-CoA is an obligate central intermediate occurring in any organism and under any physiological condition; however, this is not the case for propionyl-CoA, which is only synthesized under special physiological conditions and from only few substrates . Therefore, synthesis of 3HV-CoA requires propionyl-CoA biosynthesis. To validate our 3HV biosynthesis pathway, propionate was initially fed to provide propionyl-CoA as a precursor molecule to ensure the downstream pathway was capable of making chiral 3HV. It has been reported that the R. eutropha PHA biosynthesis genes can be functionally expressed in E. coli, resulting in homopolymer PHB production from glucose . However, low levels of 3HV monomer within the synthesized co-polymer PHBV was observed in recombinant E. coli when propionate was co-fed with glucose in a way analogous to the procedure used for R. eutropha . One explanation for the low content of 3HV monomer is that E. coli does not possess an efficient system for importing and/or converting propionate to propionyl-CoA. Therefore, to address the propionate utilization problem, a CoA-activation mechanism (encoded by the ptb-buk operon ) was incorporated into our previously developed 3HB pathway to investigate the substrate elasticity of the pathway for 3HV production.
3HV Synthesis from Glucose and 2-Ketobutyrate
In an effort to decrease acetate and increase 3HV production, several genes, including atoDA (encoding acetoacetyl-CoA transferase), poxB (encoding pyruvate oxidase), and ackA-pta (encoding acetate kinase and phosphate acetyltransferase) were deleted, and the resulting strain was designated as HCT 20. The production of (S)-3HV and (R)-3HV was further boosted to titers of 0.60 g/L and 2.04 g/L, respectively, in the recombinant acetate pathway knockout strains (HCT 20). In general, those strains produced less acetate and propionate and yielded more 3HB and 3HV compared to strains without these mutations (based on HCT 10), probably due to preserved acetyl-CoA and propionyl-CoA pools as a result of the introduced mutations. An empty-plasmid control experiment has also been conducted in the strain HCT 20 (that was not introduced with the 3HV pathway), yielding only trace amounts of acetate and propionate when grown in LB supplemented with glucose and 2-ketobutyrate (data not shown). This indicates that the production of acetate and propionate in the recombinant HCT 20 was attributed to the introduced CoA-cleaving activity conferred by ptb-buk and tesB.
3HV Synthesis from Glucose and Threonine
3HV Synthesis from Glucose
We have demonstrated the production of chiral 3HV from glucose supplemented with propionate, 2-ketobutyrate, or threonine, in recombinant E. coli. The next step is to construct a threonine over-producing strain in an attempt to achieve 3HV biosynthesis from a single carbon source. To do so, we up-regulated the threonine biosynthesis pathway by over-expressing the thrABC opeon, cloned from the wild type E. coli or the threonine producer E. coli ATCC 21277 that has a single amino acid alteration in the homoserine dehydrogenase (encoded by thrAG1297A) for relieved feedback-inhibition . Transcriptional attenuation of those genes was removed by replacing the native promoter with a T7lac promoter, allowing for IPTG-inducible expression. In addition, the pathways that compete with threonine formation as well as degrade threonine were eliminated by knocking out metA (encoding homoserine O-succinyltransferase) and tdh (encoding threonine dehydrogenase) genes, yielding strain HCT 21.
3HV Synthesis from Glycerol
Confirmation of 3HV Stereochemistry
In general, two approaches can be taken to engineer E. coli for direct 3HV production via the threonine biosynthesis pathway. The first is to utilize an existing threonine producer, such as E. coli ATCC 21277 , followed by further engineering to introduce our constructed 3HV pathway. However, this and other available threonine producing strains have typically been developed through multiple rounds of random mutagenesis and selection due to the difficulty of engineering this highly regulated and complex metabolic network. Although there are several successful cases in developing industrial threonine producers by such approaches, resultant strains usually also suffer from undesired phenotypes including, for example, growth retardation, low transformation efficiency, and by-product formation as a result of random mutations . In addition, other uncharacterized mutations may hinder further strain development as often needed. Fortunately, recent advances in computational genomics have allowed for rational development of production strains . Therefore, as a second approach, a genetically-defined threonine producing strain was established and introduced with the 3HV pathway to achieve direct microbial production of chiral 3HV from glucose or glycerol.
As seen in Figure 5 acetate is the major byproduct to the production of hydroxyacids (3HB and 3HV). In an effort to decrease acetate and increase 3HV production, a mutant strain HCT 20 with deletions on atoDA, poxB, and ackA-pta genes was developed. Counter-intuitively, the recombinant acetate pathway knockout strains of HCT 20 and HCT 21 produced slightly more acetate and less 3HB than recombinant HCT 10. We suspected that the enzymatic activity responsible for acetate production was restored by Ptb-Buk and TesB in the recombinant HCT 20 and HCT 21. In fact, in a separate experiment, both enzymes were found to have CoA-removing activities on acetyl-CoA and propionyl-CoA (data not shown), so an introduction of TesB and/or Ptb-Buk to strains HCT 20 or HCT 21 would likely restore the ability to produce acetate.
Apparently, knocking out enzymes responsible for acetate production failed to reduce acetate synthesis. Alternatively, to alleviate the substrate promiscuity of TesB and Ptb-Buk on acetyl-CoA, and thus reduce acetate production, one approach called enzyme co-localization could be implemented to allow substrate channeling between enzymes . For example, pathway enzymes of Hbd and TesB, catalyzing successive reactions, can be co-localized in an attempt to reduce the amount of freely floating TesB that may hydrolyze acetyl-CoA as well as to increase accessibility of 3HV-CoA by TesB. The spatial organization of the enzymes can be achieved using either the leucine zipper, a dimer resulting from interaction between leucine residues , or the synthetic scaffolds, constructed from protein-protein interaction domains . Furthermore, expressing enzymes that would assimilate produced acetate is another way to reduce acetate accumulation. For example, acetyl-CoA synthetase (encoded by acs) from E. coli can be over-expressed to convert acetate to acetyl-CoA with the use of one ATP. While successfully demonstrated in one work , in our case, over-expression of acs was found to have essentially no effect on acetate reduction (data not shown). Additionally, to overcome the hurdle of acetate reduction, approaches like protein engineering of TesB and/or Ptb-Buk to alleviate their substrate promiscuity, or utilization of better isozymes with more stringent substrate specificity could also mitigate the carbon loss in the form of acetate.
Among microbes, NADH and NADPH play a central role in energy metabolism by providing the cell with the reducing power for a variety of cellular redox reactions. The availability of such cofactors could impose a huge impact on the functionality of introduced biosynthetic pathways. In fact, we have previously shown that the NADPH/NADP+ ratio was two- to three-fold higher than the NADH/NAD+ ratio under the culture conditions examined, presumably affecting in vivo activities of PhaB and Hbd and resulting in greater production of (R)-3HB than (S)-3HB . Given that our proposed 3HV pathway was based on the previously established 3HB pathway, it was also expected to see the same trend of greater production of (R)-3HV than (S)-3HV, even though the cofactor dependency of 3HV synthesis may be complicated by the energetically expensive threonine biosynthesis pathway with utilization of both ATP and NADPH. In an effort to perturb the cofactor balance within the cells, thereby tuning the production of (R)-3HV and (S)-3HV, we attempted to used glycerol, a promising, abundant, and highly-reduced carbon source, to support 3HV production. Based on our calculation of reducing equivalents (e-) of glucose and glycerol, on the same basis of 2 moles of phosphoenolpyruvate synthesized, glucose and glycerol possess, respectively, 24 and 28 reducing equivalents. Potentially, the additional four reducing equivalents can be utilized to generate two NADPH or equivalent amount of ATP. In fact, it has been experimentally confirmed that a higher intracellular NADPH/NADP+ ratio was observed when glycerol was used as a carbon source than glucose, and this higher ratio was also reflected in boosted production of thymidine as its biosynthesis requires NADPH as a cofactor . Given that both NADPH and ATP play a central role in threonine biosynthesis, we hypothesized that the use of glycerol, which could generate more NADPH and ATP (Figure 1) relative to glucose, may favor threonine biosynthesis by directing more carbon flux towards production of propionyl-CoA, thus favoring the formation of 3HV relative to 3HB. In agreement with our hypothesis, a higher 3HV/3HB ratio was obtained in the (R)-3HV production when glycerol was used as the carbon source (Figure 6). In addition, much larger ratios of the total (R)-hydroxyacids (summation of (R)-3HB and (R)-3HV titers) to the total (S)-hydroxyacids (summation of (S)-3HB and (S)-3HV titers) were observed in glycerol-fed cultures (Figure 6) compared to glucose-fed cultures (Figure 5). We hypothesize that the higher intracellular NADPH/NADP+ ratio as a result of the use of glycerol would favor (R)-hydroxyacid biosynthesis compared to the use of glucose, thus yielding the larger ratios of total (R)-hydroxyacids to total (S)-hydroxyacids.
As mentioned previously, BktB was chosen as the primary thiolase due to its high enzymatic specificity towards the C5 substrate. Given that E. coli has an endogenous thiolase (encoded by atoB), a deletion of atoB was expected to increase the ratio of 3HV/3HB as AtoB has been shown to prefer to condense two molecules of acetyl-CoA instead of one propionyl-CoA and one acetyl-CoA . However, our preliminary result showed that the recombinant HCT 11 with an atoB deletion behaved exactly as the recombinant HCT 10, and the deletion in atoB had essentially no effect on 3HV production (data not shown), implying that atoB may not be a constitutively expressed gene. In addition, it is noteworthy that increased 3HV production in the recombinant HCT 20 relative to the recombinant HCT 10 was observed only with 2-ketobutyrate supplementation (Figure 3) but not with the threonine supplementation, solely glucose, or solely glycerol experiments (Figure 4, 5 and 6); similarly, an accumulation of propionate only occurred in the 2-ketobutyrate supplementation experiment (Figure 3), altogether, indicating that 3HV biosynthesis from glucose or glycerol is most likely limited by the precursor propionyl-CoA. Therefore, approaches to increase the availability of propionyl-CoA could enhance the 3HV production.
Carbon skeletons with even-chain number are naturally found in fatty acid metabolism, but those with odd-chain number are pretty novel. As a result, there is a good deal of interest in making odd-carbon chain molecules such as 3HV (C5) and propionate (C3) because they are so much harder to get to than even-carbon chain ones such as acetate (C2) and butyrate/butanol (C4). This paper opens the way for biosynthesis of the odd-carbon chain molecules from renewable feedstocks. Taking together, our work represents the first report of direct microbial production of enantiomerically pure 3HV from a single carbon source. In addition, we have explored the production of each stereoisomer of 3HV across different genetically altered E. coli strains, along with various enzyme homologs, for enhanced chiral 3HV production. Further engineering of host strains and pathway enzymes should lead to higher 3HV titers and a more economical bioprocess for the production of chiral 3HV.
E. coli strains, plasmids and oligonucleotides used
F- mcr A Δ(mrr-hsd RMS-mcr BC) ϕ80lac ZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG
Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Kanr [F' proAB lacIqZ ΔM15 Tn10 (Tetr)]
F- λ- ilvG- rfb-50 rph-1
MG1655 ΔendA ΔrecA (DE3)
MG1655 ΔendA ΔrecA ΔatoB (DE3)
MG1655 ΔendA ΔackA-pta ΔatoDA ΔpoxB (DE3)
MG1655 ΔendA ΔackA-pta ΔatoDA ΔpoxB ΔmetA Δtdh (DE3)
ColE1(pBR322) ori, lacI, T7lac, AmpR
CloDF13 ori, lacI, T7lac, StrepR
COLA ori, lacI, T7lac, KanR
pETDuet-1 harboring bktB from R. eutropha H16
pETDuet-1 harboring ptb-buk operon from C. acetobutylicum ATCC 824, and bktB from R. eutropha H16
pCDFDuet-1 harboring hbd from C. acetobutylicum ATCC 824
pCDFDuet-1 harboring tesB from E. coli MG1655, and hbd from C. acetobutylicum ATCC 824
pCDFDuet-1 harboring phaB from R. eutropha H16
pCDFDuet-1 harboring tesB from E. coli MG1655, and phaB from R. eutropha H16
pCOLADuet-1 harboring ilvA from E. coli MG1655
pCOLADuet-1 harboring ilvA from C. glutamicum
pCOLADuet-1 harboring thrABC operon from E. coli MG1655, and ilvA from C. glutamicum ATCC 13032
pCOLADuet-1 harboring thrAG1297ABC operon from E. coli ATCC 21277, and ilvA from C. glutamicum ATCC 13032
Sequence 5'→3' c
Genes derived from C. acetobutylicum ATCC 824 (hbd and ptb-buk operon), R. eutropha H16 (bktB and phaB), C. glutamicum ATCC 13032 (ilvA), E. coli MG1655 (tesB, ilvA, and thrABC opeon), and E. coli ATCC 21277 (thrAG1297ABC opeon) were obtained by polymerase chain reaction (PCR) using genomic DNA (gDNA) templates. All gDNAs were prepared using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI). Custom oligonucleotides (primers) were purchased for all PCR amplifications (Sigma-Genosys, St. Louis, MO) as listed in Table 1. In all cases, Phusion High Fidelity DNA polymerase (Finnzymes, Espoo, Finland) was used for DNA amplification. Restriction enzymes and T4 DNA ligase were purchased from New England Biolabs (Ipswich, MA). Recombinant DNA techniques were performed according to standard procedures . Three co-replicable vectors, pETDuet-1, pCDFDuet-1, and pCOLADuet-1 (Novagen, Darmstadt, Germany), were used for construction of chiral 3HV biosynthetic pathways . All vectors contain two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and a ribosome binding site (RBS), affording high-level expression of each individual gene.
Plasmids constructed in the present work are listed in Table 1. For cloning genes, PCR products incorporated with desired restriction sites within the 5' and 3' primers were digested, and the resulting DNA fragments were then cloned into pETDuet-1, pCDFDuet-1, or pCOLADuet-1. The bktB gene was inserted in between the Mfe I and Xho I sites (MCS II) of pETDuet-1 to create pET-B. The ptb-buk gene, digested from pCDF-PB with EcoRI and NotI , was inserted between the EcoR I and Not I sites (MCS I) of pET-B to create pET-PB-B. Plasmid pCDF-H was created by inserting the hbd gene between the Nde I and Avr II sites (MCS II) of pCDFDuet-1. Cloning the tesB gene between the Nco I and Not I sites (MCS I) of pCDFDuet-1 resulted in plasmid pCDF-T. Plasmid pCDF-T-H was then created by inserting the hbd gene between the Nde I and Avr II sites (MCS II) of pCDF-T. In a similar manner, plasmid pCDF-P was created by inserting the phaB gene between the Mfe I and Avr II sites (MCS II) of pCDFDuet-1. Plasmid pCDF-T-P was created by inserting the phaB gene between the Mfe I and Avr II sites (MCS II) of pCDF-T. Plasmids of pCOLA-Iec and pCOLA-Icg were constructed by inserting the E. coli ilvA and C. glutamicum ilvA, respectively, between the Nde I and Avr II sites (MCS II) of pCOLADuet-1. The thrABC operon from MG1655 was inserted in between the Nco I and Sal I sites (MCS I) of pCOLADuet-1 to create pCOLA-Tec. Plasmid pCOLA-Tec-Icg was then created by inserting the C. glutamicum ilvA gene between the Nde I and Avr II sites (MCS II) of pCOLA-Tec. To construct plasmid pCOLA-Tecm-Icg, the thrAG1997ABC operon from E. coli ATCC 21277 was inserted in between the BamH I and Sal I sites (MCS I) of pCOLA-Icg. All constructs were confirmed to be correct by restriction enzyme digestion and nucleotide sequencing.
Seed cultures of the recombinant strains were grown in LB medium at 30°C overnight on a rotary shaker at 250 rpm. For the biosynthesis of chiral 3HV, the seed cultures were used to inoculate 50 mL LB medium supplemented with 20 g/L glucose or 20 g/L glycerol at an inoculation volume of 2% in 250 mL flasks. Cultures were then incubated at 30°C on a rotary shaker until OD600 reached 0.8~1.0. At this point, 1 mM IPTG was added to the cultures to induce recombinant protein expression. Following induction, cells were cultivated at 30°C and sampled at 24 h intervals for up to 72 h post-induction for HPLC analysis. We found that both 3HB and 3HV titers did not reach a plateau until 48 h and that there was essentially no difference in the titers between 48 h and 72 h. Accordingly, only the peak titers observed at 48 h were reported in this study. In some experiments as indicated, 20 mM (~1.92 g/L) sodium propionate, 3 g/L sodium 2-ketobutyrate, or 3 g/L threonine was added into the cultures at the same time of induction. In all cases, LB medium was supplemented with 50 mg/L ampicillin, 50 mg/L streptomycin, and 30 mg/L kanamycin, as appropriate. In general, experiments were performed in triplicates, and data are presented as the averages and standard deviations of the results.
Samples were centrifuged to pellet cells while the aqueous supernatant was collected for HPLC analysis. Products of interest, including 3HB, 3HV, glucose, glycerol, 2-ketobutyrate, acetate, and propionate, were analyzed via HPLC using an Agilent 1100 series instrument equipped with a refractive index detector (RID) and a diode array detector (DAD). Given that the 3HB peak is overlapped with the glycerol peak in the RID chromatogram, detection of 3HB in the glycerol-fed cultures was achieved using the DAD at 210 nm. Analyte separation was achieved using an Aminex® HPX-87 H anion exchange column (Bio-Rad Laboratories, Hercules, CA) with 5 mM H2SO4 as the mobile phase. The mobile phase was pumped at a constant rate of 0.6 mL/min, and the column and detector temperatures were each set at 35°C throughout. Concentrations were determined by linear extrapolation from calibration of external standards.
Chiral Analysis of 3HV
The stereochemistry of 3HV produced was determined by methyl esterification of the 3HV present in the medium followed by chiral HPLC analysis as described in a previously reported method . The chiral analysis was performed on an Agilent 1100 Series instrument equipped with a Chiralcel OD-H column (0.46 cm ϕ × 25 cm) purchased from Daicel Chemical Industries (West Chester, PA). Methyl-3HV was detected on a DAD at 210 nm. The mobile phase was 9:1 n-hexane:isopropanol and the flow rate through the column was 0.7 mL/min. Due to unavailability of standards of Methyl-(R)-3HV and Methyl-(S)-3HV, these spectra were compared to a racemic 3HV standard (Epsilon Chimie, Brest, France) derivatized by methyl esterification.
We acknowledge financial support by the Synthetic Biology Engineering Research Center (SynBERC) funded by the National Science Foundation (Grant EEC-0540879), the MIT Energy Initiative (MITEI), and Shell Global Solutions (US) Inc.
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