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Figure 4 | Microbial Cell Factories

Figure 4

From: Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli

Figure 4

3HV biosynthesis from glucose and threonine. This figure shows shake-flask production of chiral 3HV by recombinant E. coli strain HCT 10 grown in LB supplemented with 20 g/L glucose and 3 g/L threonine. Chiral 3HV production using alternative threonine deaminases (encoded by ilvA) from E. coli and C. glutamicum is compared. All strains contained the same set of plasmids pET-PB-B, pCOLA-Icg or pCOLA-Iec as indicated, and pCDF-T-H (for (S)-3HV synthesis) or pCDF-T-P (for (R)-3HV synthesis).

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Microbial Cell Factories

ISSN: 1475-2859

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