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Strains
| | |
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DH10B
|
F- mcr A Δ(mrr-hsd RMS-mcr BC) ϕ80lac ZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG
|
Invitrogen
|
|
ElectroTen-Blue
|
Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Kanr [F' proAB lacIqZ ΔM15 Tn10 (Tetr)]
|
Stratagene
|
|
MG1655
|
F- λ- ilvG- rfb-50 rph-1
|
ATCC 700926
|
|
HCT 10
|
MG1655 ΔendA ΔrecA (DE3)
|
This study
|
|
HCT 11
|
MG1655 ΔendA ΔrecA ΔatoB (DE3)
|
This study
|
|
HCT 20
|
MG1655 ΔendA ΔackA-pta ΔatoDA ΔpoxB (DE3)
|
This study
|
|
HCT 21
|
MG1655 ΔendA ΔackA-pta ΔatoDA ΔpoxB ΔmetA Δtdh (DE3)
|
This study
|
|
Plasmids
| | |
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pETDuet-1
|
ColE1(pBR322) ori, lacI, T7lac, AmpR
|
Novagen
|
|
pCDFDuet-1
|
CloDF13 ori, lacI, T7lac, StrepR
|
Novagen
|
|
pCOLADuet-1
|
COLA ori, lacI, T7lac, KanR
|
Novagen
|
|
pET-B
|
pETDuet-1 harboring bktB from R. eutropha H16
|
This study
|
|
pET-PB-B a
|
pETDuet-1 harboring ptb-buk operon from C. acetobutylicum ATCC 824, and bktB from R. eutropha H16
|
This study
|
|
pCDF-H
|
pCDFDuet-1 harboring hbd from C. acetobutylicum ATCC 824
|
This study
|
|
pCDF-T-H a
|
pCDFDuet-1 harboring tesB from E. coli MG1655, and hbd from C. acetobutylicum ATCC 824
|
This study
|
|
pCDF-P
|
pCDFDuet-1 harboring phaB from R. eutropha H16
|
This study
|
|
pCDF-T-P a
|
pCDFDuet-1 harboring tesB from E. coli MG1655, and phaB from R. eutropha H16
|
This study
|
|
pCOLA-Iec
|
pCOLADuet-1 harboring ilvA from E. coli MG1655
|
This study
|
|
pCOLA-Icg
|
pCOLADuet-1 harboring ilvA from C. glutamicum
|
This study
|
|
pCOLA-Tec-Icg a
|
pCOLADuet-1 harboring thrABC operon from E. coli MG1655, and ilvA from C. glutamicum ATCC 13032
|
This study
|
|
pCOLA-Tecm-Icg a
|
pCOLADuet-1 harboring thrAG1297ABC operon from E. coli ATCC 21277, and ilvA from C. glutamicum ATCC 13032
|
This study
|
|
Primers
b
|
Sequence 5'→3'
c
| |
|
bktB_US_EcoRI
|
GAATTC ATGACGCGTGAAGTGGTAGTG
|
Sigma-Genosys
|
|
bktB_DS_XhoI
|
CTCGAG CGCAAGGCTAACCTCAGAT
|
Sigma-Genosys
|
|
hbd_US_NdeI
|
ATTCATATG AAAAAGGTATGTGTTATAGG
|
Sigma-Genosys
|
|
hbd_DS_AvrII
|
ATTCCTAGG CAGGTCGACTCTAGAACTTA
|
Sigma-Genosys
|
|
phaB_US_MfeI
|
ATTCAATTG ACGAAGCCAATCAAGGAG
|
Sigma-Genosys
|
|
phaB_DS_AvrII
|
ATTCCTAGG GGTCAGCCCATATGCAG
|
Sigma-Genosys
|
|
tesB_US_NcoI
|
ATTCCATGG GCATGAGTCAGGCGCTAA
|
Sigma-Genosys
|
|
tesB_DS_NotI
|
ATTGCGGCCGCG ACTCTAGAGACTTAATTGTG
|
Sigma-Genosys
|
|
ilvAec_US_NdeI
|
ATTACATATG GCTGACTCGCAAC
|
Sigma-Genosys
|
|
ilvAec_DS_AvrII
|
ATTACCTAGG CATTTTTCCCTAACC
|
Sigma-Genosys
|
|
ilvAcg_US_NdeI
|
ATTACATATG AGTGAAACATACGTGTC
|
Sigma-Genosys
|
|
ilvAcg_DS_AvrII
|
ATTACCTAGG CCTTCAGCTATGTTTA
|
Sigma-Genosys
|
|
thrABC_US_BamHI
|
ATTAGGATCC AAGGAGATATATCATGCGAGTGTTGAAG
|
Sigma-Genosys
|
|
thrABC_US_NcoI
|
ATTACCATGG GCATGCGAGTGTTGAAG
|
Sigma-Genosys
|
|
thrABC_DS_SalI
|
ATTAGTCGAC GATAATGAATAGATTTTACTGATG
|
Sigma-Genosys
|
