Volume 5 Supplement 1

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

Meeting abstracts

The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting.

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology.

Barcelona, Spain21-23 September 2006

Concatenated PDF 5S1

Page 2 of 3

Cellular toxicity triggered by bacterial inclusion bodies

Authors: Nuria González-Montalbán, Antonio Villaverde and Anna Aris

Citation: Microbial Cell Factories 2006 5(Suppl 1):P9

Content type: Poster Presentation Published on: 10 October 2006
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DnaK-J are limiting for proper recombinant protein folding only at low production rates and when the physiological heat-shock stress response is not triggered

Authors: Mónica Martínez-Alonso, Andrea Vera, Elena García-Fruitós, Núria González-Montalbán, Anna Arís and Antonio Villaverde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P10

Content type: Poster Presentation Published on: 10 October 2006
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Potato virus A genome-linked protein is a natively unfolded protein

Authors: Kimmo Rantalainen and Kristiina Mäkinen

Citation: Microbial Cell Factories 2006 5(Suppl 1):P11

Content type: Poster Presentation Published on: 10 October 2006
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RNA Interference mediated knockdown of genes in order to increase protein production using the baculovirus expression system

Authors: Colin Hebert, Eun Jeong Kim, Shannon F Kramer, James J Valdes and William E Bentley

Citation: Microbial Cell Factories 2006 5(Suppl 1):P12

Content type: Poster Presentation Published on: 10 October 2006
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Heterologous overexpression of a halophilic α-amylase

Authors: Vanesa Bautista, Julia Esclapez, Rosa Ma Martínez-Espinosa, Francisco Pérez-Pomares, Mónica Camacho and Ma José Bonete

Citation: Microbial Cell Factories 2006 5(Suppl 1):P13

Content type: Poster Presentation Published on: 10 October 2006
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Performance of beta-galactosidase inclusion bodies in enzymatic bioprocesses

Authors: Elena García-Fruitós, Anna Arís and Antonio Villaverde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P14

Content type: Poster Presentation Published on: 10 October 2006
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Production of recombinant mink growth hormone in E. coli

Authors: Jolanta Sereikaite, Alina Statkute, Mindaugas Morkunas, Vitaliano Borromeo, Camillo Secchi and Vladas-Algirdas Bumelis

Citation: Microbial Cell Factories 2006 5(Suppl 1):P15

Content type: Poster Presentation Published on: 10 October 2006
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Comparative analysis of E. coli inclusion bodies and thermal protein aggregates

Authors: Núria González-Montalban, Elena García-Fruitós, Salvador Ventura, Anna Arís and Antonio Villaverde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P16

Content type: Poster Presentation Published on: 10 October 2006
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Point mutation of serine 179 in the human Prolactin (PRL) affects recombinant protein expression, folding and secretion, abolishes PRL nickel (II)-binding and increases heparin binding capacities

Authors: Eric Ueda, Carlos Soares, Ameae Walker and Paolo Bartolini

Citation: Microbial Cell Factories 2006 5(Suppl 1):P17

Content type: Poster Presentation Published on: 10 October 2006
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Models for the study of inclusion bodies formation as a function of fermentation conditions and protein sequence

Authors: Pietro Gatti-Lafranconi, Diletta Ami, Antonino Natalello, Gaetano Invernizzi, Ario de Marco, Silvia Maria Doglia and Marina Lotti

Citation: Microbial Cell Factories 2006 5(Suppl 1):P18

Content type: Poster Presentation Published on: 10 October 2006
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Addition of Repressor in inducible promoter system improves soluble expression of recombinant protein in E. coli

Authors: Kyung-Hwan Jung

Citation: Microbial Cell Factories 2006 5(Suppl 1):P19

Content type: Poster Presentation Published on: 10 October 2006
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Production of proteins in Bacillus subtilis can be improved by engineering components affecting posttranslocational protein folding and degradation

Authors: Marika Vitikainen, Hanne-Leena Hyyryläinen, Anna Kivimäki, Vesa P Kontinen and Matti Sarvas

Citation: Microbial Cell Factories 2006 5(Suppl 1):P20

Content type: Poster Presentation Published on: 10 October 2006
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Production of cysteine-rich proteins in E. coli – the challenge of Wnts

Authors: Anu Mursula, Ulf Liebal and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P21

Content type: Poster Presentation Published on: 10 October 2006
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Modulation of inclusion body (IB) formation kinetics by different induction regimes in E. coli fed batch cultivations

Authors: Monika Cserjan-Puschmann, Franz Clementschitsch, Gerald Striedner, Florentiner Pötschacher, Jürgen Kern and Karl Bayer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P22

Content type: Poster Presentation Published on: 10 October 2006
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Monitoring of stress responses

Authors: Thomas Schweder, Britta Jürgen, Birgit Voigt, Daniel Pioch, Michael Hecker, Stefan Evers and Karl-Heinz Maurer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S23

Content type: Oral Presentation Published on: 10 October 2006
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Nonclassical inclusion bodies in Escherichia coli

Authors: Špela Peternel, Marjan Bele, Vladka Gaberc-Porekar and Viktor Menart

Citation: Microbial Cell Factories 2006 5(Suppl 1):P23

Content type: Poster Presentation Published on: 10 October 2006
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Interfacing Pichia pastoris cultivation with expanded bed adsorption

Authors: Mehmedalija Jahic, Josef Knoblechner, Theppanya Charoenrat, Sven-Olof Enfors and Andres Veide

Citation: Microbial Cell Factories 2006 5(Suppl 1):P24

Content type: Poster Presentation Published on: 10 October 2006
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Human Prolactin (hPRL) and Growth Hormone (hGH) distinct behavior under bacteriophage lambda P_L promoter control

Authors: Carlos RJ Soares, Eric KM Ueda, Tais L Oliveira, Susana R Heller and Paolo Bartolini

Citation: Microbial Cell Factories 2006 5(Suppl 1):P25

Content type: Poster Presentation Published on: 10 October 2006
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Two-compartment bioreactor as a scale-down model to study the effect of glucose overflow and anaerobiosis on large-scale recombinant protein production processes

Authors: Jaakko Soini, Ulla Pajulampi, Janne Sandqvist, Arne Matzen and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P26

Content type: Poster Presentation Published on: 10 October 2006
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Automated fed-batch cultivations using base consumption for real time biomass determination during production of heterologous proteins

Authors: Markus Ganzlin, Benjamin Gerwat and Philipp Garbers

Citation: Microbial Cell Factories 2006 5(Suppl 1):P27

Content type: Poster Presentation Published on: 10 October 2006
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Caspase activation, sialidase release and changes in sialylation pattern of recombinant human erythropoietin produced by CHO cells in batch and fed-batch cultures

Authors: Kok Hwee Chuan, Sing Fee Lim, Laurent Martin, Chee Yong Yun, Sophia OH Loh, Francoise Lasne and Zhiwei Song

Citation: Microbial Cell Factories 2006 5(Suppl 1):P28

Content type: Poster Presentation Published on: 10 October 2006
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Set up and optimization of a fermentation protocol for the production of a human antibody fragment (Fab') express in E. coli. Pre-pilot and cGMP pilot scale studies

Authors: E Riscaldati, A Ciabini, A Baccante, D Moscatelli, MF Errichetti, A Colagrande, S Cencioni, F Marcocci, V Di Cioccio, L Di Ciccio, M Allegretti, F Martin and G Maurizi

Citation: Microbial Cell Factories 2006 5(Suppl 1):P29

Content type: Poster Presentation Published on: 10 October 2006
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Evaluation of antifoams in the expression of a recombinant FC fusion protein in shake flask cultures of Saccharomyces cerevisiae &Pichia pastoris

Authors: William Holmes, Rodney Smith and Roslyn Bill

Citation: Microbial Cell Factories 2006 5(Suppl 1):P30

Content type: Poster Presentation Published on: 10 October 2006
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Cloning and overexpression of a yeast phytase gene in Pichia pastoris

Authors: Mélanie Ragon, Virginie Neugnot-Roux, Guy Moulin and Hélène Boze

Citation: Microbial Cell Factories 2006 5(Suppl 1):P31

Content type: Poster Presentation Published on: 10 October 2006
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Optimisation of substrate feeding in shake flask cultures of Pichia pastoris for recombinant protein production

Authors: Monika Bollok, Maria Ruottinen, Mirja Krause, Antti Vasala, Eija-Riitta Hämäläinen, Antje Neubauer, Johanna Myllyharju and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P32

Content type: Poster Presentation Published on: 10 October 2006
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Increasing the ease and speed of eukaryotic protein expression: a new cell-free in vitro translation system based on Sf insect cell extracts

Authors: Frank Schaefer, Annette Zacharias, Nicole Brinker-Krieger and Uritza von Groll

Citation: Microbial Cell Factories 2006 5(Suppl 1):P33

Content type: Poster Presentation Published on: 10 October 2006
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Impact of apoptosis gene targeting on recombinant protein glycosylation

Authors: Danny Chee Furng Wong, Niki Soo Ching Wong, John Soo Yang Goh and Miranda Gek Sim Yap

Citation: Microbial Cell Factories 2006 5(Suppl 1):P34

Content type: Poster Presentation Published on: 10 October 2006
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N-glycosylation differences between wild-type and recombinant strains affect catalytic properties of two model enzymes: β-glucosidase and phosphatase

Authors: Isabelle Mobèche, Mélanie Ragon, Guy Moulin and Hélène Boze

Citation: Microbial Cell Factories 2006 5(Suppl 1):P35

Content type: Poster Presentation Published on: 10 October 2006
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Cell engineering of Pseudoalteromonas haloplanktis TAC125: construction of a mutant strain with reduced exo-proteolytic activity

Authors: Ermenegilda Parrilli, Angela Maria Cusano, Maria Giuliani and Maria Luisa Tutino

Citation: Microbial Cell Factories 2006 5(Suppl 1):P36

Content type: Poster Presentation Published on: 10 October 2006
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Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

Authors: Georg Hinterkörner, Gudrun Brugger, Dethardt Müller, Friedemann Hesse, Renate Kunert, Hermann Katinger and Nicole Borth

Citation: Microbial Cell Factories 2006 5(Suppl 1):P37

Content type: Poster Presentation Published on: 10 October 2006
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Production of membrane proteins in yeast

Authors: Richard AJ Darby, Mohammed Jamshad, Ljuban Grgic and Roslyn M Bill

Citation: Microbial Cell Factories 2006 5(Suppl 1):P38

Content type: Poster Presentation Published on: 10 October 2006
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Fast and efficient generation of influenza A virus like particles from synthetic genes

Authors: Theresa Schinko, Haruthai Thaisuchat, Hendrik Viljoen, Nisha Padhye and Reingard Grabherr

Citation: Microbial Cell Factories 2006 5(Suppl 1):P39

Content type: Poster Presentation Published on: 10 October 2006
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Protein expression for structural studies

Authors: Yoav Peleg, Shira Albeck, Yigal Burstein, Orly Dym, Yossi Jacobovitch, Nurit Levy, Ran Meged, Yigal Michael, Jaime Prilusky, Gideon Schreiber, Israel Silman, Tamar Unger and Joel L Sussman

Citation: Microbial Cell Factories 2006 5(Suppl 1):P40

Content type: Poster Presentation Published on: 10 October 2006
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Expression and purification of the D2 dopamine receptor and the Neurokinin A receptor

Authors: Cédric Fiez-Vandal, Renaud Wagner, Franc Pattus and So Iwata

Citation: Microbial Cell Factories 2006 5(Suppl 1):P42

Content type: Poster Presentation Published on: 10 October 2006
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Efficient, antibody-mediated allosteric activation of an immobilized, E. coli beta-galactosidase recombinant biosensor

Authors: Rosa M Ferraz, Anna Arís, Gregorio Álvaro and Antonio Villaverde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P43

Content type: Poster Presentation Published on: 10 October 2006
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Cost-effective production of labeled recombinant proteins in E. coli using minimal medium

Authors: Aleksei Rozkov, Bert Larsson, Robert Björnestedt, Patrik Strömberg, Fredrik Lindqvist and Fritz Schweikart

Citation: Microbial Cell Factories 2006 5(Suppl 1):P44

Content type: Poster Presentation Published on: 10 October 2006
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Structural biology of Helicobacter pylori type IV secretion system

Authors: Alessandro Angelini, Laura Cendron, Anke Seydel, Nicola Barison, Tommaso Tosi, Roberto Battistutta and Giuseppe Zanotti

Citation: Microbial Cell Factories 2006 5(Suppl 1):P45

Content type: Poster Presentation Published on: 10 October 2006
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Expression of genes encoding membrane proteins from the hyperthermophilic Archeon Pyroccocus abyssi in Pichia pastoris

Authors: Cécile Labarre, Karine Blondeau and Herman van Tilbeurgh

Citation: Microbial Cell Factories 2006 5(Suppl 1):P46

Content type: Poster Presentation Published on: 10 October 2006
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Recombinant expression of disulfide-rich proteins: carboxypeptidase inhibitors as model proteins

Authors: Laura Sanglas, Sílvia Bronsoms, Joan L Arolas, Julia Lorenzo and Francesc X Aviles

Citation: Microbial Cell Factories 2006 5(Suppl 1):P47

Content type: Poster Presentation Published on: 10 October 2006
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Automated purification of soluble histidine tagged integrase of Tn21 expressed in E. coli cells in low amounts

Authors: Ioana Grigorescu, Anna Andersson, Markus Galin, Anita Jönsson, Anders Molin, Susanne Nyholm-Westin, Carolina Johansson, Lars Sundström and John Flensburg

Citation: Microbial Cell Factories 2006 5(Suppl 1):P48

Content type: Poster Presentation Published on: 10 October 2006
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Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter

Authors: David Resina, Monika Bollok, Francisco Valero, Peter Neubauer and Pau Ferrer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P49

Content type: Poster Presentation Published on: 10 October 2006
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Determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR

Authors: Adriano Azzoni, Elisabete Carapuça, D Miguel F Prazeres, Gabriel A Monteiro and Filipe Mergulhão

Citation: Microbial Cell Factories 2006 5(Suppl 1):P50

Content type: Poster Presentation Published on: 10 October 2006
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HYBMFA: a bioinformatics' tool for batch-to-batch bioprocess optimisation supported by elementary flux analysis

Authors: Ana Teixeira, Carlos Alves, Paula Alves, Manuel Carrondo and Rui Oliveira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P51

Content type: Poster Presentation Published on: 10 October 2006
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Application of a genome-scale metabolic model to the inference of nutritional requirements and metabolic bottlenecks during recombinant protein production in Escherichia coli

Authors: Sónia Carneiro, Isabel Rocha and Eugénio Ferreira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P52

Content type: Poster Presentation Published on: 10 October 2006
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Analysis of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated formaldehyde dehydrogenase promoter (PFLD1)

Authors: David Resina, Oriol Cos, Brigitte Gasser, Michael Mauer, Hans Marx, Michael Sauer, Francisco Valero, Diethard Mattanovich and Pau Ferrer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P53

Content type: Poster Presentation Published on: 10 October 2006
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Increasing the quality of recombinant products – Higher attraction of ribosomes leads to suppression of secondary ribosome binding sites

Authors: Ulf Liebal, Olli Niemitalo, Anu Mursula, André Juffer and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P54

Content type: Poster Presentation Published on: 10 October 2006
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Transcriptional profiling of recombinant CHO cells by a novel inter-species analysis strategy

Authors: Wolfgang Ernst, Evelyn Trummer, Hermann Katinger, Friedemann Hesse and Dethardt Müller

Citation: Microbial Cell Factories 2006 5(Suppl 1):P55

Content type: Poster Presentation Published on: 10 October 2006
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Limitations using GFP as a protein expression reporter in Pichia pastoris

Authors: Anna Surribas, David Resina, Pau Ferrer and Francisco Valero

Citation: Microbial Cell Factories 2006 5(Suppl 1):P56

Content type: Poster Presentation Published on: 10 October 2006
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Cell culture efforts to reduce glycation in recombinant humanized antibody

Authors: Inn H Yuk, Hung Huynh, Kimberly Leach, Amy Shen, Boyan Zhang, George Dutina, Patrick McKay, Amy Lim and Brad Snedecor

Citation: Microbial Cell Factories 2006 5(Suppl 1):P57

Content type: Poster Presentation Published on: 10 October 2006
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Biochemical assay development for drug discovery: a sequential optimization from protein expression to enzymatic activity

Authors: Cristina Sidoli, Angela Molteni, Beatrice Bellanti, Loredana Redaelli, Lucia Iuzzolino, Mariantonietta Rubino, Patrizia Arioli, Vanessa Nardese and Daniele Carettoni

Citation: Microbial Cell Factories 2006 5(Suppl 1):P58

Content type: Poster Presentation Published on: 10 October 2006
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The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

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