- Poster Presentation
- Open Access
Protein expression for structural studies
© Peleg et al; licensee BioMed Central Ltd. 2006
- Published: 10 October 2006
- Soluble Protein
- Molecular Chaperone
- Pichia Pastoris
- Eukaryotic Protein
Protein structure determination is an essential tool for studying protein function, and assists the design of novel drugs. The goal of the Israel Structural Proteomics Center (ISPC) is to determine the structures of proteins related to human health in their functional context http://www.weizmann.ac.il/ISPC. One of the bottlenecks encountered, primarily for eukaryotic proteins, is the production of soluble and correctly folded proteins suitable for crystallization trials. In order to overcome this obstacle, we have applied various expression strategies. E. coli is the expression system of choice, in which different parameters are tested in parallel. In cases in which post-translational modification is essential for obtaining a functional protein, eukaryotic expression systems such as Pichia pastoris or baculovirus are being employed.
Rapid evaluation of protein expression under small-scale culture conditions is essential for implementing an efficient high-throughput protein production process. Parallel utilization of a repertoire of protein expression strategies resulted in a significant increase in the number of soluble proteins obtained.
- Albeck S, Burstein Y, Dym O, Jacobovitch Y, Levi N, Meged R, Michael Y, Peleg Y, Prilusky J, Schreiber G, Silman I, Unger T, Sussman JL: Three-dimensional structure determination of proteins related to human health in their functional context at the Israel Structural Proteomics Center (ISPC). Acta Crystallogr D Biol Crystallogr. 2005, 61: 1364-72. 10.1107/S0907444905023565.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd.