Volume 5 Supplement 1

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

Meeting abstracts

The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting.

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology.

Barcelona, Spain21-23 September 2006

Concatenated PDF 5S1

Page 1 of 3

The moss bioreactor offers best of both worlds for biopharmaceutical production

Authors: Eva L Decker and Ralf Reski

Citation: Microbial Cell Factories 2006 5(Suppl 1):S1

Content type: Oral Presentation Published on: 10 October 2006
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Enhanced production of recombinant proteins by systems biotechnological approaches

Authors: Sang Yup Lee

Citation: Microbial Cell Factories 2006 5(Suppl 1):S2

Content type: Oral Presentation Published on: 10 October 2006
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Industrial aspects of protein production by filamentous fungi

Authors: Michael Ward

Citation: Microbial Cell Factories 2006 5(Suppl 1):S3

Content type: Oral Presentation Published on: 10 October 2006
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The Effects of Expressing Anti-Apoptotic Genes On Mammalian Cell Survival, Physiology, and Protein Production

Authors: Michael J Betenbaugh

Citation: Microbial Cell Factories 2006 5(Suppl 1):S4

Content type: Oral Presentation Published on: 10 October 2006
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The making and breaking of an efficient antibody factory

Authors: Roberto Sitia

Citation: Microbial Cell Factories 2006 5(Suppl 1):S5

Content type: Oral Presentation Published on: 10 October 2006
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Monitoring the Dynamics of Transcription and Translation Within the Time Course of Recombinant E. coli Cultivations

Authors: Karin Dürrschmid, Wolfgang Schmidt-Heck, Thomas Hrebicek, Helga Reischer, Norbert Auer, Gerd Margreiter, Reinhard Guthke, Andreas Rizzi and Karl Bayer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S6

Content type: Oral Presentation Published on: 10 October 2006
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Targeting expression of expanded polyglutamine proteins to the endoplasmic reticulum or mitochondria prevents their aggregation

Authors: Erwann Rousseau, Benjamin Dehay, Léa Ben-Haïem, Yvon Trottier, Michel Morange and Anne Bertolotti

Citation: Microbial Cell Factories 2006 5(Suppl 1):S7

Content type: Oral Presentation Published on: 10 October 2006
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Protein quality control systems: Mechanisms and applications

Authors: Axel Mogk and Bernd Bukau

Citation: Microbial Cell Factories 2006 5(Suppl 1):S8

Content type: Oral Presentation Published on: 10 October 2006
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Protein aggregation into bacterial inclusion bodies is a specific kinetically driven process

Authors: Natalia S de Groot, Montse Morell, Josep Vendrell, Francesc X Aviles and Salvador Ventura

Citation: Microbial Cell Factories 2006 5(Suppl 1):S9

Content type: Oral Presentation Published on: 10 October 2006
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Kinetics of aggregation and structural properties of proteins in inclusion bodies studied by Fourier transform infrared spectroscopy

Authors: Antonino Natalello, Diletta Ami, Pietro Gatti-Lafranconi, Ario de Marco, Marina Lotti and Silvia Maria Doglia

Citation: Microbial Cell Factories 2006 5(Suppl 1):S10

Content type: Oral Presentation Published on: 10 October 2006
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Investigation of the inclusion body formation process by FTIR spectroscopy

Authors: Gerd Margreiter, Manfred Schwanninger, Christian Obinger and Karl Bayer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S11

Content type: Oral Presentation Published on: 10 October 2006
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Environment matters – An E. coli case study. How process technology affects cell physiology and product quality/quantity

Authors: Ursula Rinas

Citation: Microbial Cell Factories 2006 5(Suppl 1):S12

Content type: Oral Presentation Published on: 10 October 2006
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Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production

Authors: Ramon Ramon, Oriol Cos, Pau Ferrer, JoséLuis Montesinos and Francisco Valero

Citation: Microbial Cell Factories 2006 5(Suppl 1):S13

Content type: Oral Presentation Published on: 10 October 2006
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A rational approach to optimize feed profiles for the maximization of productivity of secreted proteins expressed in Pichia pastoris

Authors: Michael Maurer, Brigitte Gasser, Manfred Kühleitner and Diethard Mattanovich

Citation: Microbial Cell Factories 2006 5(Suppl 1):S14

Content type: Oral Presentation Published on: 10 October 2006
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Expression of soluble and membrane proteins in E. coli

Authors: A James Link, Ki-Jun Jeong and George Georgiou

Citation: Microbial Cell Factories 2006 5(Suppl 1):S15

Content type: Oral Presentation Published on: 10 October 2006
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Enhancing recombinant glycoprotein yield and quality using gene targeted CHO cells lines

Authors: Danny Chee Furng Wong, Yih Yean Lee, Kathy Tin Kam Wong, Peter Morin Nissom, May May Lee and Miranda Gek Sim Yap

Citation: Microbial Cell Factories 2006 5(Suppl 1):S16

Content type: Oral Presentation Published on: 10 October 2006
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A novel engineered insect cell line for pro-protein processing and activation

Authors: Maria Cristina Sidoli, Mariantonietta Rubino, Lucia Iuzzolino, Beatrice Bellanti, Angela Molteni, Patrizia Arioli, Loredana Redaelli and Daniele Carettoni

Citation: Microbial Cell Factories 2006 5(Suppl 1):S17

Content type: Oral Presentation Published on: 10 October 2006
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Antisense RNA based control of detrimental factors for recombinant gene expression in Escherichia coli – down-regulation of RNase E

Authors: Christian Kemmer and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S18

Content type: Oral Presentation Published on: 10 October 2006
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The first fruits of an HTP membrane platform: crystal structure of the CorA Mg²⁺ transporter

Authors: Vladimir V Lunin, Elena Dobrovetsky, Galina Khutoreskaya, Rongguang Zhang, Andrzej Joachimiak, Declan A Doyle, Alexey Bochkarev, Michael E Maguire, Aled M Edwards and Christopher M Koth

Citation: Microbial Cell Factories 2006 5(Suppl 1):S19

Content type: Oral Presentation Published on: 10 October 2006
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Highly active membrane proteins produced in a cell-free expression system

Authors: Lavinia Liguori, Bruno Marques, Ana Villegas-Mendez, Romy Rothe, Norbert Rolland, Françoise Morel and Jean-Luc Lenormand

Citation: Microbial Cell Factories 2006 5(Suppl 1):S20

Content type: Oral Presentation Published on: 10 October 2006
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Production, purification and structural analysis of a cation efflux membrane protein from Thermus thermophilus

Authors: Olga Kolaj, Hui Li, Elizabeth Burrowes, Jeremy Moore, Martin Caffrey and J Gerard Wall

Citation: Microbial Cell Factories 2006 5(Suppl 1):S21

Content type: Oral Presentation Published on: 10 October 2006
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An optimized method to produce halophilic proteins in Escherichia coli

Authors: Julia Esclapez, Ma José Bonete, Mónica Camacho, Carmen Pire, Juan Ferrer, Vanesa Bautista, Rosa Ma Martínez-Espinosa, Basilio Zafrilla, Francisco Pérez-Pomares and Susana Díaz

Citation: Microbial Cell Factories 2006 5(Suppl 1):S22

Content type: Oral Presentation Published on: 10 October 2006
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Design of transcriptional fusions of stress sensitive promoters and GFP to monitor the overburden of E.coli hosts during recombinant protein production

Authors: Sabine Nemecek, Karoline Marisch, Renata Juric and Karl Bayer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S24

Content type: Oral Presentation Published on: 10 October 2006
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Dynamic optimisation of a recombinant BHK-21 culture based on elementary flux analysis and hybrid parametric/nonparametric modeling

Authors: Ana Teixeira, Carlos Alves, Paula Alves, Manuel Carrondo and Rui Oliveira

Citation: Microbial Cell Factories 2006 5(Suppl 1):S25

Content type: Oral Presentation Published on: 10 October 2006
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Monitoring of transcript regulation and protein production related stress responses in Pichia pastoris secreting Fab antibody fragments

Authors: Brigitte Gasser, Michael Maurer, Michael Sauer, Markku Saloheimo, Jari Rautio, Merja Penttila and Diethard Mattanovich

Citation: Microbial Cell Factories 2006 5(Suppl 1):S26

Content type: Oral Presentation Published on: 10 October 2006
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High level Aspergillus production of proteins

Authors: Dominique Aubert, Jan Lehmbeck, Mogens Trier Hansen and Carsten Hjort

Citation: Microbial Cell Factories 2006 5(Suppl 1):S27

Content type: Oral Presentation Published on: 10 October 2006
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Pseudomonas fluorescens – a robust expression platform for pharmaceutical protein production

Authors: Diane Retallack, J Carrie Schneider, Lawrence Chew, Tom Ramseier, Jeffrey Allen, Anant Patkar, Charles Squires, Henry Talbot and Jon Mitchell

Citation: Microbial Cell Factories 2006 5(Suppl 1):S28

Content type: Oral Presentation Published on: 10 October 2006
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Enhanced protein expression through strain selection, gene disruption, improved vector design and co-expression of endogenous chaperones

Authors: Darrell Sleep, Chris Finni and Leslie Evans

Citation: Microbial Cell Factories 2006 5(Suppl 1):S29

Content type: Oral Presentation Published on: 10 October 2006
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Impact of high throughput technology on recombinant protein production

Authors: Jo J Jones, Angela M Bridges, Andrew P Fosberry, Jonathan Bullock, Ian Hudson, Richard M Hall, Owen Jenkins and Jeffrey A Cole

Citation: Microbial Cell Factories 2006 5(Suppl 1):S30

Content type: Oral Presentation Published on: 10 October 2006
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Industrial scale production of chymosin with Aspergillus niger

Authors: Karsten Hellmuth

Citation: Microbial Cell Factories 2006 5(Suppl 1):S31

Content type: Oral Presentation Published on: 10 October 2006
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Filamentous fungi as cell factories for protein production

Authors: Peter J Punt

Citation: Microbial Cell Factories 2006 5(Suppl 1):S32

Content type: Oral Presentation Published on: 10 October 2006
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Genome-wide analysis of protein production physiology in the filamentous fungus Trichoderma reesei

Authors: Markku Saloheimo, Tiina Pakula, Mikko Arvas, Jari Rautio and Merja Penttilä

Citation: Microbial Cell Factories 2006 5(Suppl 1):S33

Content type: Oral Presentation Published on: 10 October 2006
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Expression efficiency and sequence-based factors: a comparative view on 79 human genes in Pichia pastoris

Authors: Christine Lang and Mewes Böttner

Citation: Microbial Cell Factories 2006 5(Suppl 1):S34

Content type: Oral Presentation Published on: 10 October 2006
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A novel yeast expression system based on a hormone-induced transcriptional cascade

Authors: María José Quintero, Miguel Arévalo-Rodríguez, Ángel Cebolla and Sebastián Chávez

Citation: Microbial Cell Factories 2006 5(Suppl 1):S35

Content type: Oral Presentation Published on: 10 October 2006
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Use of a "universal" yeast vector (CoMed™) system for the production of proteins in Hansenula polymorpha and Arxula adeninivorans

Authors: Gerd Gellissen, Gerhard Steinborn and Gotthard Kunze

Citation: Microbial Cell Factories 2006 5(Suppl 1):S36

Content type: Oral Presentation Published on: 10 October 2006
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Recombinant protein expression system in cold loving microorganisms

Authors: Rosanna Papa, Valentina Rippa, Giovanni Sannia, Gennaro Marino and Angela Duilio

Citation: Microbial Cell Factories 2006 5(Suppl 1):S37

Content type: Oral Presentation Published on: 10 October 2006
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Rapid detection of bacteriophage infection and prophage induction using electric biochips

Authors: Marcin Los, Joanna Los and Grzegorz Wegrzyn

Citation: Microbial Cell Factories 2006 5(Suppl 1):S38

Content type: Oral Presentation Published on: 10 October 2006
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NIisin Controlled gene Expression (NICE) in Lactococcus lactis - versatile applications ranging from membrane proteins to large scale processes

Authors: Igor Mierau

Citation: Microbial Cell Factories 2006 5(Suppl 1):S39

Content type: Oral Presentation Published on: 10 October 2006
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An integral process for the production of virus-like particles by insect cells

Authors: Laura A Palomares and Jimmy A Mena

Citation: Microbial Cell Factories 2006 5(Suppl 1):S40

Content type: Oral Presentation Published on: 10 October 2006
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Fast generation of high producer cho cell lines by an iterative transfection process

Authors: P-A Girod, M Grandjean, D Calabrese, D Martinet, J Beckmann and N Mermod

Citation: Microbial Cell Factories 2006 5(Suppl 1):S41

Content type: Oral Presentation Published on: 10 October 2006
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Zera^®, a novel technology for stable accumulation and easy recovery of recombinant proteins in eukaryotic protein-production hosts

Authors: I Llop, M Torrent, P Marzabal, M Bastida, B Llompart and D Ludevid

Citation: Microbial Cell Factories 2006 5(Suppl 1):S42

Content type: Oral Presentation Published on: 10 October 2006
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Design of improved membrane protein production experiments in yeast: quantitation of the host response

Authors: Nicklas Bonander, Kristina Hedfalk, Christer Larsson, Petter Mostad, Celia Chang, Lena Gustafsson and Roslyn M Bill

Citation: Microbial Cell Factories 2006 5(Suppl 1):S43

Content type: Oral Presentation Published on: 10 October 2006
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Comparative transcriptional profiling of the bacterial stress response in temperature and chemically-induced recombinant E. coli processes

Authors: Daniela Böhm and Ursula Rinas

Citation: Microbial Cell Factories 2006 5(Suppl 1):P1

Content type: Poster Presentation Published on: 10 October 2006
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FT-IR spectroscopy for the study of bacterial membrane stress induced by recombinant protein production

Authors: Diletta Ami, Antonino Natalello, Pietro Gatti-Lafranconi, Tina Schultz, Marina Lotti, Ario de Marco and Silvia Maria Doglia

Citation: Microbial Cell Factories 2006 5(Suppl 1):P2

Content type: Poster Presentation Published on: 10 October 2006
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Regulation of the secretion pathway of CHO cells for altered recombinant Mab production rates during the course of MTX amplification

Authors: Yuan Sheng Yang, Janet Chusainow, Yan Ying Mao, Steven CL Ho and Miranda GS Yap

Citation: Microbial Cell Factories 2006 5(Suppl 1):P3

Content type: Poster Presentation Published on: 10 October 2006
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Microarray-based analysis of recombinant protein production in E. coli

Authors: Ronan O'Dwyer, Xuejun Hu, Mattia Pelizzola, Olga Kolaj, Maria Foti, Paola Ricciardi-Castagnoli and JGerard Wall

Citation: Microbial Cell Factories 2006 5(Suppl 1):P4

Content type: Poster Presentation Published on: 10 October 2006
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A sensor of the Unfolded Protein Response to study the stress induced in Yarrowia lipolytica strains by the production of heterologous proteins

Authors: Catherine Madzak and Jean-Marie Beckerich

Citation: Microbial Cell Factories 2006 5(Suppl 1):P5

Content type: Poster Presentation Published on: 10 October 2006
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Native and heterologous protein oxidation and subsequent degradation in a recombinant filamentous fungus Aspergillus niger B1-D

Authors: Qiang Li, Linda M Harvey and Brian McNeil

Citation: Microbial Cell Factories 2006 5(Suppl 1):P6

Content type: Poster Presentation Published on: 10 October 2006
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Low growth temperatures improve the conformational quality of aggregation prone recombinant proteins in both soluble and insoluble E. coli cell fractions

Authors: Andrea Vera, Núria González-Montalbán, Elena Garcia-Fruitós, Anna Arís and Antonio Villaverde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P7

Content type: Poster Presentation Published on: 10 October 2006
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Zea mays L. transglutaminase expression in Escherichia coli

Authors: Patricia Carvajal, Enrique Villalobos, Alexandre Campos, JMa Torné, Eduard Barberà and Mireya Santos

Citation: Microbial Cell Factories 2006 5(Suppl 1):P8

Content type: Poster Presentation Published on: 10 October 2006
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The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

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