- Poster Presentation
- Open Access
FT-IR spectroscopy for the study of bacterial membrane stress induced by recombinant protein production
Microbial Cell Factories volume 5, Article number: P2 (2006)
Microorganisms respond to environmental stresses regulating their membrane fluidity by changing the lipid fatty acid composition. In particular, in bacterial cells the alteration of membrane lipid composition plays an important role in response to heat and toxic stresses [1, 2]. It is known that at increasing temperatures the regulation of membrane fluidity occurs through the incorporation of more saturated fatty acids, longer acyl chains and, in some cases, by changing the unsaturated fatty acids from cis to trans conformation. Among different stresses, production of heterologous proteins is of particular interest for its applications in biotechnology.
In this work we show the potential of FT-IR microspectroscopy to monitor changes in membrane composition during recombinant protein production. In particular, we studied the infrared absorption of E. coli strains expressing several proteins with different amount of soluble and aggregated fractions. In addition, as a control experiment, we have studied E. coli strains expressing beta galactosidase, as reporter gene, under the chaperone IbpB promoter .
In all model systems examined, an increase in the band intensities at 2850 cm-1 and at 2925 cm-1 was observed in presence of a high level of stress . As these two bands are due to CH2 stretching vibrations , this spectral behaviour may indicate that longer acyl chain and /or more saturated fatty acids are incorporated in cell membranes. The protein expression analysis indicates that the enzymes that lead to the accumulation of Acetyl Co-A (precursor of fatty acids) are strongly accumulated whereas the phospholipide degradation seems being inhibited.
Indeed, when the protein is expressed also in a soluble form – as indicated by the FT-IR protein response in the amide I region and confirmed by the SDS-PAGE analysis – the two CH2 bands are more intense than in the case of IB formation. This behaviour seems to indicate that the presence of the soluble recombinant protein induces a stress in the cell, suggesting that IBs could limit the toxicity of overexpressed foreign proteins.
Yuk HG, Marshall DL: Heat adaptation alters Escherichia coli O157:H7 membrane lipid composition and verotoxin production. Appl Environ Microbiol. 2003, 69: 5115-5119. 10.1128/AEM.69.9.5115-5119.2003.
Heipieper HJ, Meinhardt F, Segura A: The cis-trans isomerase of unsaturated fatty acids in Pseudomonas and Vibrio: biochemistry, molecular biology and physiological function of a unique stress adaptive mechanism. FEMS Microbiol Lett. 2003, 229: 1-7. 10.1016/S0378-1097(03)00792-4.
Lesley SA, Graziano J, Cho CY, Knuth MW, Klock HE: Gene expression response to misfolded protein as a screen for soluble recombinant protein. Protein Eng. 2002, 15: 153-160. 10.1093/protein/15.2.153.
Schuster KC, Mertens F, Gapes JR: FT-IR spectroscopy applied to bacterial cells as a novel method for monitoring complex biotechnological processes. Vibrational Spectroscopy. 1999, 19: 467-477. 10.1016/S0924-2031(98)00058-7.
Arrondo JLR, Goni FM: Infrared studies of protein-induced perturbation of lipids in lipoproteins and membranes. Chem Phys Lipids. 1998, 96: 53-68. 10.1016/S0009-3084(98)00080-2.
Ami D, Bonecchi L, Calì S, Orsini G, Tonon G, Doglia SM: FT-IR study of heterologous protein expression in recombinant Escherichia coli strains. Biochim Biophys Acta. 2003, 1624: 6-10.
Ami D, Natalello A, Gatti-Lafranconi P, Lotti M, Doglia SM: Kinetics of inclusion body formation studied in intact cells by FT-IR spectroscopy. FEBS Lett. 2005, 579: 3433-3436. 10.1016/j.febslet.2005.04.085.
Ami D, Natalello A, Taylor G, Tonon G, Doglia SM: Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy. Biochim Biophys Acta. 2006
This work was supported by INFM (Istituto Nazionale Fisica della Materia) grant to SMD.
About this article
Cite this article
Ami, D., Natalello, A., Gatti-Lafranconi, P. et al. FT-IR spectroscopy for the study of bacterial membrane stress induced by recombinant protein production. Microb Cell Fact 5, P2 (2006). https://doi.org/10.1186/1475-2859-5-S1-P2
- Saturated Fatty Acid
- Membrane Fluidity
- Infrared Absorption
- Recombinant Protein Production
- Aggregate Fraction