Skip to main content


We're creating a new version of this page. See preview

  • Poster Presentation
  • Open Access

Comparative transcriptional profiling of the bacterial stress response in temperature and chemically-induced recombinant E. coli processes

  • 1 and
  • 1
Microbial Cell Factories20065 (Suppl 1) :P1

  • Published:


  • Basic Fibroblast Growth Factor
  • Stress Responsive Gene
  • Recombinant Protein Production
  • Hybridisation Event
  • Coli Process


Production of heterologous proteins results in a number of metabolic and physiological changes in the host cells during the course of a production process, namely the induction of stress responses and corresponding alterations in gene expression profiles [1].


This study focuses on quantitative monitoring of the adaptation of E. coli to recombinant protein production on the transcriptome level by a bead-based RNA sandwich hybridisation assay, a rapid novel method based on the detection of hybridisation events between specific oligonucleotide probes and the target nucleic acids [2, 3].

The expression profiles of selected genes including the product gene, anabolic and stress responsive genes were quantitatively analyzed in cells producing the human basic fibroblast growth factor (hFGF-2), a protein that partially aggregates into inclusion bodies. Transcriptome profiles during temperature- and IPTG-induced synthesis of hFGF-2 using the K12 strain TG1 and BL21(DE3) as production hosts, respectively, were compared.

Authors’ Affiliations

GBF – German Research Centre for Biotechnology, Braunschweig, Germany


  1. Hoffmann F, Rinas U: Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Eng Biotechnol. 2004, 89: 73-92.Google Scholar
  2. Gabig-Ciminska M, Holmgren A, Andresen H, Barken K, Wümpelmann M, Albers J, Hintsche R, Breitenstein A, Neubauer P, Los M, et al.: Electric chips for rapid detection and quantification of nucleic acids. Biosens Bioelectron. 2004, 19: 537-546. 10.1016/S0956-5663(03)00273-2.View ArticleGoogle Scholar
  3. Soini J, Falschlehner C, Mayer C, Böhm D, Panula J, Vasala A, Neubauer P: Transient increase of ATP as a response to temperature up-shift in Escherichia coli. Microb Cell Fact. 2005, 4: 9- 10.1186/1475-2859-4-9.View ArticleGoogle Scholar


© Böhm and Rinas; licensee BioMed Central Ltd. 2006

This article is published under license to BioMed Central Ltd.