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Comparative transcriptional profiling of the bacterial stress response in temperature and chemically-induced recombinant E. coli processes
Microbial Cell Factories volume 5, Article number: P1 (2006)
Production of heterologous proteins results in a number of metabolic and physiological changes in the host cells during the course of a production process, namely the induction of stress responses and corresponding alterations in gene expression profiles .
This study focuses on quantitative monitoring of the adaptation of E. coli to recombinant protein production on the transcriptome level by a bead-based RNA sandwich hybridisation assay, a rapid novel method based on the detection of hybridisation events between specific oligonucleotide probes and the target nucleic acids [2, 3].
The expression profiles of selected genes including the product gene, anabolic and stress responsive genes were quantitatively analyzed in cells producing the human basic fibroblast growth factor (hFGF-2), a protein that partially aggregates into inclusion bodies. Transcriptome profiles during temperature- and IPTG-induced synthesis of hFGF-2 using the K12 strain TG1 and BL21(DE3) as production hosts, respectively, were compared.
Hoffmann F, Rinas U: Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Eng Biotechnol. 2004, 89: 73-92.
Gabig-Ciminska M, Holmgren A, Andresen H, Barken K, Wümpelmann M, Albers J, Hintsche R, Breitenstein A, Neubauer P, Los M, et al.: Electric chips for rapid detection and quantification of nucleic acids. Biosens Bioelectron. 2004, 19: 537-546. 10.1016/S0956-5663(03)00273-2.
Soini J, Falschlehner C, Mayer C, Böhm D, Panula J, Vasala A, Neubauer P: Transient increase of ATP as a response to temperature up-shift in Escherichia coli. Microb Cell Fact. 2005, 4: 9- 10.1186/1475-2859-4-9.
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Böhm, D., Rinas, U. Comparative transcriptional profiling of the bacterial stress response in temperature and chemically-induced recombinant E. coli processes. Microb Cell Fact 5, P1 (2006). https://doi.org/10.1186/1475-2859-5-S1-P1
- Basic Fibroblast Growth Factor
- Stress Responsive Gene
- Recombinant Protein Production
- Hybridisation Event
- Coli Process