Zea mays L. transglutaminase expression in Escherichia coli
© Carvajal et al; licensee BioMed Central Ltd. 2006
Published: 10 October 2006
Transglutaminase (protein-glutamine:amine γ-glutamyl-transferase, E.C. 184.108.40.206) catalyses acyl-transfer reactions between γ-carboxyamide groups of glutamine residues and the ε -amino group of lysines in proteins, leading to inter- or intramolecular cross-linking. Transglutaminases (TGs) have been found in mammals, plants, fish, nematodes and bacteria. Two maize cDNA clones (TGZ15 and TGZ21) that expressed active transglutaminase localized in chloroplasts were isolated [1, 2].
TGZ4p was expressed in E. coli mainly as inclusion bodies. The purification under denaturing conditions and refolding in vitro was a suitable procedure to obtain functional TG.
Thanks to the Spanish National Project MCYT BFI2003-003318 and CEUB-IQS fellowship.
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