- Poster Presentation
- Open Access
Regulation of the secretion pathway of CHO cells for altered recombinant Mab production rates during the course of MTX amplification
© Yang et al; licensee BioMed Central Ltd. 2006
- Published: 10 October 2006
- Gene Copy Number
- Secretion Pathway
- Stable Cell Line
- Representative Gene
Monoclonal antibody (Mab) production by mammalian cells is a complex, multiple-step process which is regulated at transcriptional, translational, and post-translational levels. A detailed understanding of how cells regulate this pathway is a prerequisite for designing genetic strategies for increasing antibody production . Methotrexate (MTX), which is widely used in the creation of high-producing stable cell lines by amplification of gene copy number, provides an effective means to alter Mab production rates for mechanistic studies of the regulation of this pathway .
In this work, stable CHO DG44 cell lines expressing a human anti-D Mab were created and single-cell clones were amplified to obtain a series of cultures with varying production rates. During the course of amplification, changes in the Mab gene copy numbers, transcriptional levels of Mab mRNAs, and accumulated intracellular Mab peptides were examined for each clone. In addition, changes in expression levels of representative genes with function in translation, folding, assembly, and degradation were determined. Gene copy number and transcription level were quantified by quantitative real time PCR, and the intracellular Mab peptides were quantified by western blotting and ELISA.
Results obtained in this work could help identify the rate-limiting steps and factors that are significant in limiting production rate for high-producing clones.
We thank Toh Poh Choo and Jessna Yeo who created the cell lines used in this work.
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This article is published under license to BioMed Central Ltd.