Monitoring of stress responses
© Schweder et al; licensee BioMed Central Ltd. 2006
Published: 10 October 2006
The consideration of bacterial stress and starvation responses is of great importance for the successful establishment of an industrial large scale fermentation process. Suitable analysis techniques for stress and starvation specific genes are therefore particularly interesting for the monitoring and control of such processes. The combined methods of transcriptome analysis, high resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry have been extensively applied for the description of general and specific stress and starvation responses of industrial microorganisms.
It is demonstrated that electric chips loaded with mRNA specific DNA-probes or with marker protein specific antibodies represent a suitable alternative for gene expression analyses in competition with the real time RT-PCR during fermentation processes. The electric chip technique is easy to automate and could be cheaper in the handling than the established gene expression analysis techniques. The electric biochip combined with an automated sample preparation establishes a basis for continuous at-line monitoring of host cell physiology during industrial bioprocesses.
This study was financially supported by the Ministry of Education, Science and Culture of Mecklenburg-Vorpommern (grant number: 0202120) and the Federal Ministry of Education, and Science (BMBF) (grant number: 031U213A).
- Voigt B, Schweder T, Sibbald MJ, Albrecht D, Ehrenreich A, Bernhardt J, Feesche J, Maurer KH, Gottschalk G, Van Dijl JM, Hecker M: The extracellular proteome of Bacillus licheniformis grown in different media and under different nutrient starvation conditions. Proteomics. 2006, 6: 268-281. 10.1002/pmic.200500091.View ArticleGoogle Scholar
- Jürgen B, Barken KB, Tobisch S, Pioch D, Wümpelmann M, Hecker M, Schweder T: Application of an Electrical DNA-Chip for the Expression Analysis of Bioprocess-Relevant Genes of Bacillus subtilis. Biotechnol Bioeng. 2005, 92: 299-307. 10.1002/bit.20578.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd.