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Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters
Microbial Cell Factories volume 17, Article number: 25 (2018)
Genome sequencing revealed that Streptomyces sp. can dedicate up to ~ 10% of their genomes for the biosynthesis of bioactive secondary metabolites. However, the majority of these biosynthetic gene clusters are only weakly expressed or not at all. Indeed, the biosynthesis of natural products is highly regulated through integrating multiple nutritional and environmental signals perceived by pleiotropic and pathway-specific transcriptional regulators. Although pathway-specific refactoring has been a proved, productive approach for the activation of individual gene clusters, the construction of a global super host strain by targeting pleiotropic-specific genes for the expression of multiple diverse gene clusters is an attractive approach.
Streptomyces albus J1074 is a gifted heterologous host. To further improve its secondary metabolite expression capability, we rationally engineered the host by targeting genes affecting NADPH availability, precursor flux, cell growth and biosynthetic gene transcriptional activation. These studies led to the activation of the native paulomycin pathway in engineered S. albus strains and importantly the upregulated expression of the heterologous actinorhodin gene cluster.
Rational engineering of Streptomyces albus J1074 yielded a series of mutants with improved capabilities for native and heterologous expression of secondary metabolite gene clusters.
Talented bacteria, characterized by tens of secondary metabolite gene clusters, dedicate ~ 10% of their genomes to the corresponding biosynthetic functions . The continuously reducing cost of genome sequencing renders the detection of diverse biosynthetic gene clusters rather a routine in a natural product research lab. What still hampers the large scale discovery and characterization of new bioactive molecules, however, is the expression of these gene clusters either in native or heterologous hosts. The biosynthesis of natural products is highly regulated and gene clusters often remain silent until suitable conditions are met. The expression of secondary metabolite pathways is under the control of tight and complex hierarchical regulatory networks that integrate multiple nutritional and environmental signals perceived by pleiotropic and/or cluster-situated transcriptional regulators (CSRs) . Although pathway-specific refactoring and CSR-engineering has been proved to be a productive approach for the activation of individual gene clusters [3, 4], the need for generation of orthogonal sets of promoters and repressor sites can be laborious and challenging. A more broadly applicable approach would be more desirable where pleiotropic regulators affecting more than one biosynthetic pathway are targeted to amplify their positive signals in favor of secondary metabolism.
Streptomyces albus J1074 is widely used for heterologous expression studies due to its minimized genome (6.8 Mb) that allows fast growth . The strain has been used to express diverse gene clusters and proved to be a preferred host for expression of metagenomic DNA clones encoding secondary metabolites [6,7,8,9,10]. Importantly, organic extracts from routine laboratory S. albus fermentation broths lack any endogenous secondary metabolites. Therefore, S. albus J1074 is an ideal strain for further optimization of its gene expression capabilities by targeted genetic engineering. Development of genetic engineering protocols for the activation of native gene clusters could also be useful for heterologous expression of foreign gene clusters in the same engineered host. Previously, a number of S. albus native metabolites have been characterized by targeted engineering of their corresponding cryptic gene clusters . Not much effort, however, has been dedicated to the awakening of these metabolic pathways by modulating global regulators, which may have broader applicability for the heterologous expression of diverse biosynthetic gene clusters using S. albus as a host.
With the broad heterologous expression efficacy in mind, S. coelicolor M145 derivative strains have been previously constructed where endogenous gene clusters have been deleted to alleviate precursor competition and mutations to ribosomal proteins and RNA polymerase have been incorporated for higher transcriptional and translational fidelity . On the other hand, pleiotropic regulators can be targeted to optimize the expression of natural products. Many global regulators have been identified in S. coelicolor, a model strain for genetic studies, and in the industrially important strains S. avermetilis and S. griseus (Table 1) [13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36]. For example, phosphofructokinase (pfk) has been shown to link primary and secondary metabolisms. The observed antibiotic downregulatory role of wblA may also be a secondary result of its direct role in the primary metabolism. Other pleiotropic DNA binding regulators like cAMP receptor protein (CRP) can recognize cognate binding sites in multiple gene clusters in response to not yet identified signals or, as in the case of DasR, can modulate their binding activity in response to glycosylated sugars. Additional global regulators can induce secondary metabolism in response to nutrient starvation and phosphate limitation.
We aimed to generate a series of engineered S. albus strains harboring multiple targeted pleiotropic gene modifications that would enhance secondary metabolite production. The selection of the candidate global regulators to be modified was based on the following criteria. (1) They should affect secondary metabolite overproduction in diverse ways (e.g., increased precursor supply and biosynthetic gene transcriptional activation); (2) they should not be members of the same regulon; (3) a candidate regulator should act consistently either as activator or repressor of secondary metabolism in most Streptomyces strains studied. For strain improvement purposes, gene deletions of protein repressors are preferred from overexpression of activators because there is no need to maintain multiple selection markers during genetic engineering experiments.
In this study we deleted pfk and wblA homologues in the S. albus J1074 genome yielding single and double mutants. The resulting mutant strains were further engineered by the introduction of the crp gene from S. coelicolor under the control of strong constitutive ermE* promoter to assess the potential cumulative effects towards the expression of native and heterologous gene clusters.
Bacterial strains and media used
Bacterial strains used in this work were S. albus J1074 and derivative mutants constructed in this study. Escherichia coli EPI300 (Epicentre) and S17.1 strains were used for subcloning and intergeneric conjugation, respectively. Growth medium for S. albus was tryptone soy broth (TSB) for genomic DNA isolation, and mannitol-soy flour agar (MS) was used for sporulation and R5A as regular production medium. LB medium was used for routine E. coli growth. When plasmid-containing clones were grown, media were supplemented with appropriate antibiotics: ampicillin (100 µg/ml), hygromycin (100 µg/ml), apramycin (50 µg/ml), chloramphenicol (12.5 µg/ml), when required.
S. albus J1074 genomic DNA isolation and fosmid DNA library construction
Genomic DNA was isolated from S. albus mycelia collected from 2-day cultures grown in TSB. The mycelia pellet was lysed with lysozyme solution (0.5 M sucrose, 25 mM Tris–HCl, 5 mM EDTA and 2 mg/ml lysozyme) at 37 °C for 30 min. EDTA and SDS were added to 50 mM and 0.5% (final concentration) respectively. After thorough mixing, 1/3 volume of phenol/chloroform was added and mixed to emulsify. The mixture was spun at 10,000 rpm for 10 min at 4 °C and DNA was precipitated from the aqueous phase with 0.7 vol isopropanol in the presence of 1/10 vol sodium acetate, pH 5.5. The DNA pellet was washed with 70% ethanol, air-dried and resuspended in TE buffer. Fosmid DNA library was constructed using the CopyControl HTP fosmid production kit (Epicentre) following the manufacturer’s instructions.
In-frame phosphofructokinase pfk SA gene (XNR_1407) deletion
A suicide vector for Streptomyces gene deletions through homologous recombination was constructed based on pUC19 (New England Biolabs) (Fig. 1). The vector was digested with ScaI and EcoRI and ligated with DraI–EcoRI fragment from pOJ436 carrying the oriT-apramycin cassette. Primers ALpfk1 5′-ATCGGGATCCTGGTCGACAACGCGATGGAGG-3 and ALpfk2 5′-AGCAGGAGAGACAGCACGATGTGAACCGGCTCCGCGCACACG-3′ were used to amplify 1 kb flanking region downstream of pfk gene. Primers ALpfk3 5′-CGTGTGCGCGGAGCCGGTTCACATCGTGCTGTCTCTCCTGCT-3′ and ALpfk4 5′-ATCGAAGCTTGCCCAGCAGAACCGTTCCGTC-3′ were used to amplify 1 kb flanking region upstream of pfk gene. Engineered restriction sites are underlined in the primer sequences and the start/stop codon fusion site is in italics. Standard 20 µl PCR reaction mix contained 1× G buffer (Epicentre), 50 pmol of each primer, 2.5 UTaq polymerase (New England BioLabs), and 100 ng gDNA. A 2-step PCR protocol was used with the following conditions: 1 cycle at 95 °C followed by 30 cycles consisting of 40 s at 95 °C and 3 min at 72 °C, followed by a final extension at 72 °C for 5 min. The two PCR products were gel-purified and used as a template for overlapping PCR (same protocol as before) with primers ALpfk1 and 4 to generate a 2 kb fragment. The PCR product was gel-purified and digested with BamHI and HindIII and ligated to the described suicide vector that has been similarly digested. E. coli S17.1 strain was transformed with the resulting plasmid and used for conjugal transfer into S. albus using published protocols . Apramycin resistant colonies carrying single crossover were streaked on MS agar plates with no selection for sporulation. Spores were diluted to yield single colonies and spread on MS agar plates. Double crossover mutants were identified by replica plating using Difco nutrient agar (DNA) plates with/out apramycin selection (10 µg/ml). Correct deletion of the target gene in the mutant chromosome was further verified via PCR amplification using primers ALpfkconfF 5′-GAGGTCGGCATCTCCCGCATC-3′ and ALpfkconfR 5′-ACTCCGACGATACCGGTGCG-3′. PCR reaction mix was the same as before and PCR protocol was 1 cycle at 95 °C followed by 30 cycles consisting of 40 s at 95 °C, 40 s at 58 °C and 40 s at 72 °C, followed by a final extension at 72 °C for 5 min.
In-frame wblA SA gene (XNR_2735) deletion
The S. albus fosmid library was screened by PCR using primers ALwblAF 5′-CCATCGGCACGTACCTGGCC-3′ and ALwblAR 5′-ATGTCCTTCCTGTCCCCGGC-3′. A single fosmid containing the full-length wblASA gene was recovered by PCR screening of serially diluted, PCR-positive library pools. PCR reaction mix contained 1× G buffer (Epicentre), 50 pmol of each primer, 2.5 UTaq polymerase (New England BioLabs) and 1 µl of the corresponding library pool and the PCR protocol was 1 cycle at 95 °C followed by 30 cycles consisting of 40 s at 95 °C, 40 s at 57 °C and 40 s at 72 °C, followed by a final extension at 72 °C for 5 min. The wblA ortholog was deleted using λ-mediated recombineering approach (Fig. 2). The wblASA-specific aac(3)IV-oriT resistance cassette flanking by two FRT sites was amplified from pIJ773 using primers SAwblAredF 5′-TGGGGGAGCCTCGATTCGGGAGAGGACGGCGCCGGTATGATTCCGGGGATCCGTCGACC-3′ and SawblAredR 5′-GGTTCCCGTACTCCTCGCTCGCCCTTGCCGGCCGGTCTATGTAGGCTGGAGCTGCTTC-3′. The amplified cassette was transformed into E. coli BW25113/pKD46 containing the recovered wblASA-containing fosmid and transformants were selected on apramycin and chloramphenicol LB agar plates. Gene replacement was confirmed by PCR analysis of the mutated (ΔwblASA) fosmid using ALwblAF/R primers. To generate seamless gene deletion, the mutated fosmid was transformed into E. coli EL250 strain expressing FLP recombinase that catalyzes the recombination between the FRT sites. Following induction with l-arabinose, the excision of the apramycin resistant cassette was detected by patching single colonies on LB agar plates with/out 50 µg/ml apramycin. In-frame deletion mutants were verified by PCR using ALwblAF/R primers as before. The confirmed mutated fosmid was retrofitted with oriT-apramycin cassette by λ-mediated recombineering using primers pCCFRedF 5′-GTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCCGCAACGTTGTTGCCATTGC-3′ and pCCFRedR 5′-AGCGATGAGCTCGGACTTCCATTGTTCATTCCACGGACAAATCCCCGATCCGCTCCACG-3′. The cassette was amplified from pOJ436. The pCCF2 cloning vector sites targeted for recombination are underlined in the primer sequences. The final retrofitted and mutated wblASA-containing fosmid was introduced into E. coli S17.1 cells for conjugation into S. albus. Double crossover mutants were confirmed by PCR using ALwblAF/R primers.
Overexpression of crp SC gene from S. coelicolor M145 (SCO3571) in S. albus J1074
A crp overexpression plasmid was made by cloning the crpSC gene and its downstream sequence immediately downstream of the ermE* promoter in the pIJ10257 vector. The crp coding sequence was PCR amplified from S. coelicolor M145 gDNA using primers CrpF 5′-GAGAACTCATATGGACGACGTTC-3′ and CrpR 5′-CGTAAGCTTGGCCTAGGTCGCAGGGAC-3′. Engineered NdeI and HindIII sites in the forward and reverse primer, respectively, is underlined. PCR cycling conditions were 1 cycle at 95 °C followed by 30 cycles consisting of 40 s at 95 °C, 40 s at 58 °C and 40 s at 72 °C, followed by a final extension at 72 °C for 5 min. PCR product was gel-purified, digested with NdeI/HindIII and ligated to similarly digested pIJ10257 plasmid. Recombinant plasmid was introduced into E. coli S17.1 cells for conjugation into S. albus. Exconjugants were selected on MS agar plates supplemented with 25 µg/ml nalidixic acid and 50 µg/ml hygromycin.
Knock-out of paulomycin gene cluster
For the isolation of paulomycin gene cluster, the S. albus fosmid library was screened by PCR using primers pml10F 5′-GGGATTCCCTGAGCGGAGTAC-3′ and pml10R 5′-GGTTTCCAGGGGCCCTTCTAG-3′. A single fosmid containing pml1–pml19 genes (entire gene cluster contains 42 genes) was recovered by PCR screening of serially diluted, PCR-positive library pools. PCR conditions were the same as used for wblASA—containing fosmid isolation. The recovered plm fosmid was digested with XhoI restriction enzyme and subsequently self-ligated to eliminate 13 genes out of 19 cloned pml genes including pml10 pathway-specific regulator required for the transcriptional activation of the gene cluster (Fig. 5). The resulting minimized plm fosmid was retrofitted with oriT-apramycin cassette by recombineering as before and introduced into E. coli S17.1 cells for conjugation into S. albus and derivative mutants. Double crossover mutants were confirmed by PCR using ΔpaulconfF 5′-GAAACCGCTCCGTCCGTCCGACACC-3′ and ΔpaulconfR 5′-TGCATCCGCAGCACCAGCAGG-3′ primers. PCR conditions were 1 cycle at 95 °C followed by 30 cycles consisting of 40 s at 95 °C, 40 s at 60 °C and 40 s at 72 °C, followed by a final extension at 72 °C for 5 min.
Cloning and site-specific integration of actinorhodin gene cluster into S. albus J1074 and derivative strains
For the isolation of actinorhodin gene cluster, the S. coelicolor fosmid library was screened by PCR using primers Act85F 5′-CTTAAATCCTCGAAGGCGAC-3′ and Act85R 5′-GCGCCCATCAGTTTGGCGTG-3′. PCR conditions were 1 cycle at 95 °C followed by 30 cycles consisting of 40 s at 95 °C, 40 s at 55 °C and 40 s at 72 °C, followed by a final extension at 72 °C for 5 min. Four PCR-positive single clones were recovered. Two clones contained partial actinorhodin gene cluster and the other two harbored actinorhodin gene cluster with different sizes of flanking regions. The fosmid with the largest DNA sequence flanking the entire actinorhodin gene cluster (SCO5067–SCO5104) was subsequently retrofitted with oriT-ApraR cassette. For that, pOJ436 plasmid was double digested with PmlI–SmaI and 1.8 kb fragment containing the cassette was gel-purified and ligated with PsiI-digested and dephosphorylated actinorhodin-containing fosmid. Correct recombinant fosmid was confirmed by PCR using primers Act85F/R as above and used to transform E. coli S17.1 cells for conjugation into S. albus strains. Blue-pigmented exconjugants were easily selected on MS agar plates supplemented with 25 µg/ml nalidixic acid and 50 µg/ml apramycin and verified for the actinorhodin integration by PCR.
Actinorhodin production and antimicrobial assays
Three biological replicates were tested using three confirmed colonies from each conjugation of actinorhodin cluster into S. albus J1074, S. albus+pIJ10257ermE*crp and S. albus Δpfk+pIJ10257ermE*crp. These colonies were streaked on MS agar plates to yield fully confluent spore lawns. Following 6 days of incubation at 30 °C, the agar from each plate was cut into small pieces and immersed into 50 ml of 1 M KOH. The tubes were left overnight at 4 °C with agitation. The samples were then spun at 4000×g for 10 min and the absorbance of the supernatant was measured at 640 nm. Actinorhodin concentration was calculated according to the Lambert–Beer’s law using molar extinction coefficient of 25,320/M/cm that corresponds to pure actinorhodin .
Bacillus cereus overnight cultures grown in LB were diluted by 106-fold. Aliquots (100 μl) of the diluted culture were added to individual wells of a 96-well plate starting from the second column. Diluted culture (195 µl) were then added to the wells of the first column. Crude organic extracts were resuspended in methanol at 20 mg/ml. These solutions (5 µl) were added to the wells of the first column in the microtiter plate and then serially diluted twofold per well across the plate. The plates were incubated at 30 °C for 18–24 h. Concentration of 500, 250, 125, 62.5, 31.25, 15.6, 7.8, 3.9, 1.95, 0.97, 0.48, 0.24 μg/ml were tested for each crude extract. The final methanol concentration was kept at 2.5%. Minimum inhibitor concentrations are reported as the lowest concentration at which no bacterial growth was observed.
Diamide sensitivity assays
Lawns of S. albus J1074 wild-type and Δpfk mutant were generated by overlaying R5A plates (sucrose 100 g/l, K2SO4 0.25 g/l, MgCl2 10.12 g/l, glucose 10 g/l, casamino acids 0.1 g/l, yeast extract 5 g/l, MOPS 21 g/l, NAOH 2 g/l, R2YE trace elements 2 ml/l, 15 g/l agar) with 3 ml soft Nutrient Agar containing 107 fresh spores. Immediately after plating, paper discs soaked in 100 mM diamide were added and plates were incubated at 30 °C for 24 h.
Crude extract production for screening
Crude extracts for screening purposes were generated from 50-ml cultures of S. albus strains and derivative mutants grown in R5A liquid media. After 6 days of growth, cultures were extracted twice with an equal volume of ethyl acetate and the dried extracts were then used for screening.
LC–MS profiling of engineered S. albus secondary metabolite content
The crude extracts were dissolved in LC–MS grade methanol and centrifuged for 30 min. The resulting clear supernatant (10 μl) was used for LC–MS analysis. A SHIMADZU Prominence UPLC system fitted with an Agilent Poroshell 120 EC-C18 column (2.7 μm, 4.6 × 50 mm) coupled with a Linear Ion Trap Quadrupole LC/MS/MS Mass Spectrometer system was used in the studies. Acetonitrile (B)/water (A) containing 0.1% formic acid were used as mobile phases with a linear gradient program (10–99% solvent B over 40 min) to separate chemicals by the above reverse phase HPLC column. The column at 30 °C was eluted first with 10% solvent B (acetonitrile with 0.1% formic acid) for 3 min and then with a linear gradient of 10–50% solvent B in 15 min, followed by another linear gradient of 50–99% solvent B in 12 min. After eluting in 99% solvent B for 5 min, the linear gradient of 99–10% solvent B in 1 min was used. The column was further re-equilibrated with 10% solvent B for 4 min. The flow rate was set as 0.5 ml/min, and the products were detected by a PDA detector. For MS detection, the turbo spray conditions included curtain gas: 30 psi; ion spray voltage: 5500 V; temperature: 600 °C; ion source gas 1:50 psi; ion source gas 2:60 psi. For MS/MS analysis, the collision energy was 12 eV.
Construction of S. albus engineered strains
We used SCO5426 as the first gene probe encoding one of the three phosphofructokinases found in the S. coelicolor genome that is shown to upregulate actinorhodin through increased carbon flux into the pentose phosphate pathway . Blast analysis identified the orthologue gene in S. albus genome that shares 89% nucleotide homology, designated as pfkSA. The left-side flanking regions of pfkSA are conserved in both strains as the gene is located next to a cluster of three genes (phosphate acetyltransferase, acetate kinase and pyruvate kinase) involved in pyruvate metabolism. Similarly, we probed wblASA that showed 87% identity to wblASC and SCO1712SA with 76% sequence homology to SCO1712SC. We constructed an in-frame deletion of pfkSA using a pUC19-based suicide vector where the ampicillin resistance gene was replaced with oriT-apramycin cassette for transfer and selection in Streptomyces. The vector harbors two fragments of ~ 1 kb upstream and downstream flanking regions of pfk gene that have been fused together at start and stop codons of the gene by overlapping PCR. The plasmid was conjugated into S. albus and double crossover mutants were verified by PCR of ApraS colonies (Fig. 1a, b). Wild type and ΔpfkSA showed no difference when they grew on MS plates but ΔpfkSA mutant was less sensitive to diamide (Fig. 1c). Diamide is an artificial thiol oxidant that forms protein intramolecular disulfide bonds. The reduction of these toxic disulfide bonds is achieved through the action of thioredoxin/thioredoxin reductase system in the presence of NADPH . Similar to pfk deletion in S. coelicolor, carbon flux towards pentose phosphate pathway due to pfk deletion may result in a higher level of NADPH that makes S. albus Δpfk mutant more resistant to diamide oxidant. For generating wblASA deletion mutant, we constructed a fosmid library of S. albus J1074 and screened for wblA sequences. A single fosmid containing the full-length gene was recovered and ReDirect protocol  was used to replace wblASA gene with apramycin marker flanked by FRT sites, which was subsequently removed by FLP recombinase resulting in fosmid with seamless wblASA deletion (Fig. 2a, b). The resulting mutagenized fosmid was conjugated into S. albus and exconjugants were PCR-screened for double crossover mutant identification (Fig. 2a, b). Similar to wblA deletion phenotypes reported in other Streptomyces spp. [13, 41,42,43], the ΔwblASA mutants failed to sporulate (Fig. 2c). ΔwblASA mutants also accumulated more biomass (~ 5-fold) relative to wild type when they grew in R5A media (Fig. 4b). Multiple attempts to knock out SCO1712SA using either Red/ET recombineering or CRISPR-Cas9 systems were proved fruitless, suggesting that SCO1712SA may have an essential role in S. albus growth cycle. In order to combine the positive role of crp global regulator on secondary metabolism  to the above mutants, we heterologously expressed the crp gene from S. coelicolor into S. albus chromosome under the control of a strong constitutive promoter. The coding region of crpSC was PCR-amplified, cloned into pIJ10257 conjugative integrative plasmid downstream of ermE*p and transferred in S. albus derivative strains using hygromycin selection (Fig. 3a). The overexpression of crpSC gene had no effect on the growth rate of S. albus in R5A liquid media (Fig. 4b) but interfered with the sporulation process resulting in a white phenotype relative to wild type when S. albus grew on MS solid media (Fig. 3b).
Profiling of the secondary metabolite content in engineered S. albus strains
Wild type and mutant strains of S. albus were grown in R5A media for 6 days and culture extracts were analyzed by LC–MS (Fig. 4a). Although the metabolic profile of Δpfk strain did not differ from that of wild type, ΔwblA and [+ermE*p-crpSC] strains produced a set of metabolites that were absent in wild type extracts. The most dominant peaks (1–4) appeared at 13 min, 14 min, 20 min and 21 min. The [M + Na] masses of 670, 684, 795 and 809 respectively matched those of paulomenol B/A and paulomycin B/A, respectively. In addition compounds 1/2 and 3/4 showed absorption spectra with maxima at 322 and 275 nm identical to paulomenols and paulomycin, respectively. In order to genetically verify the production of paulomenols/paulomycins in ΔwblA and [+ermE*crpSC] strains, we deleted a 15-kb region from the paulomycin (plm) gene cluster that includes the pathway-specific regulator plm10 (Fig. 5a, b). These corresponding peaks disappeared from the culture extracts of resultant strains in LC–MS analysis (Fig. 5c; Additional file 1: Figure S1). It is difficult to quantitate the production of paulomycins because of their partial degradation to paulomenols. Indeed we observed the transition from paulomycin to paulomenols when analyzing samples daily following inoculation from fermentation broths (Additional file 1: Figure S2). Nonetheless, based on the most dominant paulomenol B peak (peak #1) and the biomass produced by the engineered strains, there was a 2-fold increase in the production rate of the paulomenol B per mg dry biomass in [+ermE*crpSC] background relative to ΔwblA mutation. Paulomycins differ from paulomenols by the presence of paulic acid that gives the characteristic UV absorption maxima at 275 nm and confers antimicrobial activity against Gram-positive bacteria. Extracts of ΔwblA and [+ermE*crpSC] strains prepared after 4 days of growth in R5A media were active against Bacillus cereus (as low as 7.8 µg/ml) as opposed to extracts derived from wild type and Δplm backgrounds that they showed no antimicrobial activity up to 500 µg/ml tested (Fig. 5d).
Using Regulatory Sequence Analysis Tools (RSAT; http://rsat.eu/) , we scanned the paulomycin gene cluster for possible CRP binding sites using the two reported sequence motifs, GTG(N)6GNCAC and the one with more relaxed binding specificity GTG(N)6GNGAN . The first motif was found within the coding sequence of plm12, 28, 29, 35, 37 and plm40 genes while the second motif found within the coding sequences of plm2, 4, 6–10, 12, 23, 28, 32 and plm42 genes as well in the intergenic region plm7–plm8 genes (Additional file 1: Table S1). Genes plm2 and plm10 are two of the four transcriptional regulators found in the paulomycin cluster and specifically for plm2 gene putative CRP binding site starts five bases upstream of its start codon whereas overlaps the start codon of plm42 encoding for dTDP-4-keto-6-deoxyhexose 3,5-epimerase starting nine bases upstream of the corresponding start codon (Additional file 1: Tables S1, S2).
Heterologous expression of actinorhodin in S. albus engineered strains
Next we wanted to test the effects of the gene-targeted engineering of S. albus genome on the expression of heterologous gene clusters. For proof of principle, we used the model actinorhodin gene cluster encoding a diffusible pH-sensing pigment. A single fosmid harboring the gene cluster including flanking regions (SCO5067–SCO5104) was recovered from S. coelicolor M145 DNA library and retrofitted with oriT-integrase-apramycin cassette derived from pOJ436 vector. The resulting fosmid was transferred into S. albus mutant strains by intergeneric conjugation. The wblASA sporulation-deficient phenotype was not ideal to function as a recipient strain in intergeneric conjugations for routine transfer of foreign gene clusters due to very low transfer rates when using mycelia fragments. Therefore, we restricted the heterologous expression assay on the [ermE*crpSC] single mutant and Δpfk+ermE*crpSC double mutant. Increased production of actinorhodin was observed that followed the corresponding sequentially accumulated gene modifications wt < [+ermE*crpSC] < Δpfk+ermE*crpSC (Fig. 6). The transcriptional control of crpSC gene copy over actinorhodin gene cluster expression in S. coelicolor has already been established . In the S. albus genetic context, overexpression of crpSC gene improved the heterologous expression of actinorhodin by 1.6-fold followed by an additional 1.2-fold when combined with the pfkSA deletion, indicating the approximately additive effect of these mutations to the actinorhodin biosynthesis.
The biosynthesis of natural products is highly regulated and gene clusters often remain silent until suitable conditions are met. The expression of secondary metabolite pathways is under the control of tight and complex hierarchical regulatory networks that integrate multiple nutritional and environmental signals perceived by pleiotropic and pathway-specific transcriptional regulators. Several successful strategies have been employed in the past, targeting both groups of regulators for activation of silent pathways in many Streptomyces species. S. albus J1074 is a gifted strain for heterologous expression of secondary metabolite biosynthetic genes of broad (meta)genomic origin. Given the current plethora of sequence availability for many diverse biosynthetic gene clusters, further improvement of this strain utilizing not only pathway-specific mutagenesis but broad host engineering approaches could result in a promising drug discovery platform.
Recent studies have linked oxidative stress with antibiotic production in Streptomyces coelicolor [45,46,47] where secondary metabolite production could function as a homeostatic response to overactive cellular oxidative phosphorylation. Elevated levels of NADPH and ATP, induction of disulfide stress-responsive genes are all accompanied with the onset of antibiotic production. Phosphofructokinase (pfk) functions at the branching point between glycolysis and pentose phosphate pathway (PPP). Deletion of its gene in S. coelicolor confers more resistance to the thiol oxidant diamide than the wild-type strain and results in overexpression of actinorhodin by diverting fructose-6-phosphate into PPP leading to increased levels of NADPH cofactor that is necessary for the function of many biosynthetic redox enzymes . Avermectin production is correlated with increased activity of pentose phosphate pathway in S. avermetilis . S. hygroscopicus Δpfk mutant increases rapamycin production by 30.8% . Here we showed that S. albus Δpfk strain is more resistant to diamide than the wild type. Therefore, S. albus Δpfk strain more likely overexpresses the pentose phosphate pathway resulting in higher intracellular NADPH availability that allows the mutant to cope more efficiently with the oxidative stress than the wild-type. The increased supply of the NADPH cofactor allows this heterologous host to improve the production of herbicidal thaxtomins, whose biosynthesis relies on the NADPH-dependent function of cytochrome P450 enzymes (unpublished data).
WblA, member of the WhiB-like proteins, has also a negative effect on disulfide stress response . This family of proteins contains a [Fe-S] structural element that converts them into redox sensors. One of the key characteristics of wblA gene deletion in S. coelicolor is the limited sporulation and prolonged fast growth . WblA is shown to be involved in downregulation of antibiotic production. In fact, deletion of wblA homologue genes in S. ghanaensis, S. peucetius, S. somaliensis, S. venezuelae, S. ansochromogenes resulted in the overexpression of moenomycin, doxorubicin, violapyrone B, pikromycin and tylosin analogs biosynthetic genes respectively, suggesting that wblA functions as a down-regulator for secondary metabolites biosynthesis in Streptomyces species [39, 40, 50,51,52]. AdpA, another global regulator of secondary metabolism, is shown to repress the expression of wblA in S. coelicolor indicating that the two regulators are members of the same regulon . Our results indicate that deletion of wblA in S. albus blocked completely the sporulation process and the ΔwblA strain overproduced paulomycins A/B during fermentation, which finally ended in the accumulation of paulomenols A/B after 6 days of growth. The increased biomass observed in the S. albus ΔwblA mutant may also contribute to the production enhancement of paulomycins previously undetected by analytical techniques. The induced production of paulomycins may be a cellular response to utilize the increased energy levels generated during the extended fast growth of this mutant, which is not favorable for the cell during nutrient limitation.
cAMP receptor protein (CRP) has been extensively studied in E. coli for its involvement in carbon catabolite repression. In Streptomyces, deletion of the gene results in defects in germination and sporulation . Exogenous addition of cAMP in S. coelicolor stimulated actinorhodin production  and it has been shown that CRP protein recognizes its cognate binding sites in 8 out of the 22 biosynthetic gene clusters in S. coelicolor genome affecting their gene expression, as well as promoter region of acetyl-CoA carboxylase that generates malonyl-CoA precursor for secondary metabolism . CRP-transcriptionally targeted genes are also cross-modulated by two component regulatory cascades such as PhoR–PhoP and AfsK–AfsR–AfsS that link phosphate homeostasis and antibiotic production in Streptomyces . Overexpression of crpSC gene in S. albus produced a white phenotype and induced the production of paulomycin-related metabolites with actually higher specific rate than the ΔwblA strain. Putative CRPsc binding sites are detected in the paulomycin cluster providing a genetic framework for the modulation of paulomycin gene expression by this global regulator.
Heterologous expression is an efficient way to discover new metabolites when the original producer is not known or is not genetically amenable. While ΔwblA non-sporulating phenotype does not allow the efficient transfer and heterologous expression of large gene clusters, here we demonstrated that pfkSA gene deletion (even though it does not have any apparent effect on the S. albus native metabolome) plays a positive role in the heterologous expression of actinorhodin and acts synergistically with crpSC gene, yielding a total ~ 2-fold increase in actinorhodin production relative to the wild-type S. albus expressing the act genes. S. albus has a recorded ability to heterologously express other type II polyketides . Nae et al.  have constructed a S. coelicolor ΔpfkΔwblAΔSCO1712 triple mutant that also showed a synergistic effect in stimulating actinorhodin and other type II polyketide biosynthesis . We attempted to delete SCO1712 homologue gene in S. albus but our efforts were unsuccessful using both recombineering (REDIRECT method) and CRISPR/Cas9 approaches. Interestingly, we were able to obtain single cross-over mutants using REDIRECT method, potentially suggesting that complete elimination of the gene through a double cross-over event is not possible due to the essential role of SCO1712SA in S. albus growth. Deletion of SCO1712 in S. coelicolor results in overproduction of actinorhodin and Red antibiotics and affects morphological differentiation by earlier formation of aerial mycelium and sporulation deficient phenotypes. However, this gene is dispensable for growth in S. coelicolor . Given the natural ability of S. albus to express type II PKS genes, the constructed strains with cleaner background described here like ΔpfkΔplm+ermE*crpSC will be proved useful tools for the characterization of novel type II polyketides.
Gene-targeted engineering of S. albus J1074 genome resulted in improved gene expression capabilities of secondary metabolism. Deletion of pfk gene supplied increased levels of NADPH reducing cofactor to the biosynthetic pathways containing NADPH-dependent enzymatic steps. Heterologous expression of actinorhodin was assisted by this genetic modification. Overexpression of the transcriptional regulator CRP from S. coelicolor in the S. albus background activated the expression of paulomycins and function synergistically with global regulators controlling other modes of regulation of secondary metabolism like pfk for the heterologous expression of actinorhodin. Deletion of the global antibiotic down regulator WblA, induced the production of paulomycins in response to prolong fast growth and biomass accumulation. Overall we showed that rational, multiplex genome engineering (Fig. 7) is an efficient way to unlock the expression of native metabolites and further enhance the heterologous expression properties of gifted hosts.
nicotinamide adenine dinucleotide phosphate (reduced form)
ethylene diamine tetraacetic acid
whiB-like gene A
cAMP receptor protein
Baltz RH. Renaissance in antibacterial discovery from actinomycetes. Curr Opin Pharmacol. 2008;8:557–63.
Liu G, Chater KF, Chandra G, Niu G, Tan H. Molecular regulation of antibiotic biosynthesis in Streptomyces. Microbiol Mol Biol Rev. 2013;77:112–43.
Tan GY, Deng K, Liu X, Tao H, Chang Y, Chen J, Chen K, Sheng Z, Deng Z, Liu T. Heterologous biosynthesis of spinosad: an omics-guided large polyketide synthase gene cluster reconstitution in Streptomyces. ACS Synth Biol. 2017;6:995–1005.
Shao Z, Rao G, Li C, Abil Z, Luo Y, Zhao H. Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold. ACS Synth Biol. 2013;2:662–9.
Zaburannyi N, Rabyk M, Ostash B, Fedorenko V, Luzhetskyy A. Insights into naturally minimised Streptomyces albus J1074 genome. BMC Genom. 2014;15:97.
Baltz RH. Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters. J Ind Microbiol Biotechnol. 2010;15:759–72.
Lombó F, Velasco A, Castro A, de la Calle F, Braña AF, Sánchez-Puelles JM, Méndez C, Salas JA. Deciphering the biosynthesis pathway of the antitumor thiocoraline from a marine actinomycete and its expression in two Streptomyces species. ChemBioChem. 2006;15:366–76.
Bilyk O, Sekurova ON, Zotchev SB, Luzhetskyy A. Cloning and heterologous expression of the grecocycline biosynthetic gene cluster. PLoS ONE. 2016;11:e0158682.
Kallifidas D, Kang HS, Brady SF. Tetarimycin A, an MRSA-active antibiotic identified through induced expression of environmental DNA gene clusters. J Am Chem Soc. 2012;134:19552–5.
Feng Z, Kallifidas D, Brady SF. Functional analysis of environmental DNA-derived type II polyketide synthases reveals structurally diverse secondary metabolites. Proc Natl Acad Sci USA. 2011;108:12629–34.
Olano C, García I, González A, Rodriguez M, Rozas D, Rubio J, Sánchez-Hidalgo M, Braña AF, Méndez C, Salas JA. Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074. Microb Biotechnol. 2014;7:242–56.
Gomez-Escribano JP, Bibb MJ. Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters. Microb Biotechnol. 2011;4:207–15.
Fowler-Goldsworthy K, Gust B, Mouz S, Chandra G, Findlay KC, Chater KF. The actinobacteria-specific gene wblA controls major developmental transitions in Streptomyces coelicolor A3(2). Microbiology. 2011;157:1312–28.
Kang SH, Huang J, Lee HN, Hur A, Cohen SN, Kim ES. Interspieces DNA microarray analysis identifies WblA as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces. J Bacteriol. 2007;189:4315–9.
Lee HN, Kim JS, Kim P, Lee HS, Kim ES. Repression of antibiotic down-regulator by AdpA in Streptomyces coelicolor. Appl Environ Microbiol. 2013;79:4159–63.
Higo A, Hara H, Horinouchi S, Ohnishi Y. Genome-wide distribution of AdpA, a global regulator for secondary metabolism and morphological differentiation in Streptomyces, revealed the extent and complexity of the AdpA regulatory network. DNA Res. 2012;193:259–73.
Rigali S, Titgemeyer F, Barends S, Mulder S, Thomae AW, Hopwood DA, van Wezel GP. Feast or famine: the global regulator DasR links nutrient stress to antibiotic production by Streptomyces. EMBO Rep. 2008;9:670–5.
Li X, Yu T, He Q, McDowall KJ, Jiang B, Jiang Z, Wu L, Li G, Li Q, Wang S, Shi Y, Wang L, Hong B. Binding of a biosynthetic intermediate to AtrA modulates the production of lidamycin by Streptomyces globisporus. Mol Microbiol. 2015;96:1257–71.
van Wezela Gilles P, McDowall Kenneth J. The regulation of the secondary metabolism of Streptomyces: new links and experimental advances. Nat Prod Rep. 2011;28:1311–33.
Borodina I, Siebring J, Zhang J, Smith CP, van Keulen G, Dijkhuizen L, Nielsen J. Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by phosphofructokinase deletion. J Biol Chem. 2008;283:25186–99.
Dang L, Liu J, Wang C, Liu H, Wen J. Enhancement of rapamycin production by metabolic engineering in Streptomyces hygroscopicus based on genome-scale metabolic model. J Ind Microbiol Biotechnol. 2017;44:259–70.
Horinouchi S. AfsR as an integrator of signals that are sensed by multiple serine/threonine kinases in Streptomyces coelicolor A3(2). J Ind Microbiol Biotechnol. 2003;30:462–7.
Sola-Landa A, Moura RS, Martin JF. The two-component PhoRPhoP system controls both primary metabolism and secondary metabolite biosynthesis in Streptomyces lividans. Proc Natl Acad Sci USA. 2003;100:6133–8.
Martín JF, Rodríguez-García A, Liras P. The master regulator PhoP coordinates phosphate and nitrogen metabolism, respiration, cell differentiation and antibiotic biosynthesis: comparison in Streptomyces coelicolor and Streptomyces avermitilis. J Antibiot. 2017;70:534–41.
Horinouchi S. A microbial hormone, A-factor, as a master switch for morphological differentiation and secondary metabolism in Streptomyces griseus. Front Biosci. 2002;7:d2045–57.
Ando N, Matsumori N, Sakuda S, Beppu T, Horinouchi S. Involvement of afsA in A-factor biosynthesis as a key enzyme. J Antibiot. 1997;50:847–52.
Gao C, Mulder D, Yin C, Elliot MA. Crp is a global regulator of antibiotic production in Streptomyces. MBio. 2012;3:e00407–12.
McKenzie NL, Nodwell JR. Phosphorylated AbsA2 negatively regulates antibiotic production in Streptomyces coelicolor through interactions with pathway-specific regulatory gene promoters. J Bacteriol. 2007;189:5284–92.
Shu D, Chen L, Wang W, Yu Z, Ren C, Zhang W, Yang S, Lu Y, Jiang W. afsQ1–Q2-sigQ is a pleiotropic but conditionally required signal transduction system for both secondary metabolism and morphological development in Streptomyces coelicolor. Appl Microbiol Biotechnol. 2009;81:1149–60.
Hesketh A, Sun J, Bibb M. Induction of ppGpp synthesis in Streptomyces coelicolor A3(2) grown under conditions of nutritional sufficiency elicits actII-ORF4 transcription and actinorhodin biosynthesis. Mol Microbiol. 2001;39:136–44.
Jin W, Ryu YG, Kang SG, Kim SK, Saito N, Ochi K, Lee SH, Lee KJ. Two relA/spoT homologous genes are involved in the morphological and physiological differentiation of Streptomyces clavuligerus. Microbiology. 2004;150:1485–93.
Hu H, Zhang Q, Ochi K. Activation of antibiotic biosynthesis by specified mutations in the rpoB gene (encoding the RNA polymerase beta subunit) of Streptomyces lividans. J Bacteriol. 2002;184:3984–91.
Wang G, Hosaka T, Ochi K. Dramatic activation of antibiotic production in Streptomyces coelicolor by cumulative drug resistance mutations. Appl Environ Microbiol. 2008;74:2834–40.
Koshla O, Lopatniuk M, Rokytskyy I, Yushchuk O, Dacyuk Y, Fedorenko V, Luzhetskyy A, Ostash B. Properties of Streptomyces albus J1074 mutant deficient in tRNALeuUAA gene bldA. Arch Microbiol. 2017. https://doi.org/10.1007/s00203-017-1389-7.
Chater KF, Chandra G. The use of the rare UUA codon to define “expression space” for genes involved in secondary metabolism, development and environmental adaptation in Streptomyces. J Microbiol. 2008;46:1–11.
Lee HN, Huang J, Im JH, Kim SH, Noh JH, Cohen SN, Kim ES. Putative TetR family transcriptional regulator SCO1712 encodes an antibiotic downregulator in Streptomyces coelicolor. Appl Environ Microbiol. 2010;76:3039–43.
Kieser T, Bibb MJ, Chater K, Hopwood DA. Practical Streptomyces genetics. Norwich: Norwich Research Park; 2000.
Bystrykh L, Fernandez-Moreno MA, Herrema JK, Malpartida F, Hopwood DA, Dijkhuizen L. Production of actinorhodin-related “blue pigments” by Streptomyces coelicolor A3(2). J Bacteriol. 1996;178:2238–44.
Paget MS, Kang JG, Roe JH, Buttner MJ. sigmaR, an RNA polymerase sigma factor that modulates expression of the thioredoxin system in response to oxidative stress in Streptomyces coelicolor A3(2). EMBO J. 1998;17:5776–82.
Gust B, Challis GL, Fowler K, Kieser T, Chater KF. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci USA. 2003;100:1541–6.
Huang X, Ma T, Tian J, Shen L, Zuo H, Hu C, Liao G. wblA, a pleiotropic regulatory gene modulating morphogenesis and daptomycin production in Streptomyces roseosporus. J Appl Microbiol. 2017;123:669–77.
Lu C, Liao G, Zhang J, Tan H. Identification of novel tylosin analogues generated by a wblA disruption mutant of Streptomyces ansochromogenes. Microb Cell Fact. 2015;14:173.
Rabyk M, Ostash B, Rebets Y, Walker S, Fedorenko V. Streptomyces ghanaensis pleiotropic regulatory gene wblA(gh) influences morphogenesis and moenomycin production. Biotechnol Lett. 2011;33:2481–6.
Medina-Rivera A, Defrance M, Sand O, Herrmann C, Castro-Mondragon JA, Delerce J, Jaeger S, Blanchet C, Vincens P, Caron C, Staines DM, Contreras-Moreira B, Artufel M, Charbonnier-Khamvongsa L, Hernandez C, Thieffry D, Thomas-Chollier M, van Helden J. RSAT 2015: regulatory sequence analysis tools. Nucleic Acids Res. 2015;43:W50–6.
Coze F, Gilard F, Tcherkez G, Virolle MJ, Guyonvarch A. Carbon-flux distribution within Streptomyces coelicolor metabolism: a comparison between the actinorhodin-producing strain M145 and its non-producing derivative M1146. PLoS ONE. 2013;8(12):e84151.
Esnault C, Dulermo T, Smirnov A, Askora A, David M, Deniset-Besseau A, Holland IB, Virolle MJ. Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145. Sci Rep. 2017;7:200.
Tierrafría VH, Cuauhtemoc LC, Nidia MC, Alba RR, Sara CL, Esteban M, Romina RS, Ruiz-Villafán B, Nielsen LK, Sánchez S. Deletion of the hypothetical protein SCO2127 of Streptomyces coelicolor allowed identification of a new regulator of actinorhodin production. Appl Microbiol Biotechnol. 2016;100:9229–37.
Ikeda H, Kotaki H, Tanaka H, Omura S. Involvement of glucose catabolism in avermectin production by Streptomyces avermitilis. Antimicrob Agents Chemother. 1988;32:282–4.
Kim JS, Lee HN, Kim P, Lee HS, Kim ES. Negative role of wblA in response to oxidative stress in Streptomyces coelicolor. J Microbiol Biotechnol. 2012;22:736–41.
Huang H, Hou L, Li H, Qiu Y, Ju J, Li W. Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66. Microb Cell Fact. 2016;15:116.
Noh JH, Kim SH, Lee HN, Lee SY, Kim ES. Isolation and genetic manipulation of the antibiotic down-regulatory gene, wblA ortholog for doxorubicin-producing Streptomyces strain improvement. Appl Microbiol Biotechnol. 2010;86(4):1145–53.
Woo Min-Woo, Nah Hee-Ju, Choi Si-Sun, Kim Eung-Soo. Pikromycin production stimulation through antibiotic down-regulatory gene disruption in Streptomyces venezuelae. Biotechnol Bioprocess Eng. 2014;19:973–7.
Derouaux A, Halici S, Nothaft H, Neutelings T, Moutzourelis G, Dusart J, Titgemeyer F, Rigali S. Deletion of a cyclic AMP receptor protein homologue diminishes germination and affects morphological development of Streptomyces coelicolor. J Bacteriol. 2004;186:1893–7.
Süsstrunk U, Pidoux J, Taubert S, Ullmann A, Thompson CJ. Pleiotropic effects of cAMP on germination, antibiotic biosynthesis and morphological development in Streptomyces coelicolor. Mol Microbiol. 1998;30:33–46.
Iqbal HA, Low-Beinart L, Obiajulu JU, Brady SF. Natural product discovery through improved functional metagenomics in Streptomyces. J Am Chem Soc. 2016;138:9341.
Nah JH, Kim HJ, Lee HN, Lee MJ, Choi SS, Kim ES. Identification and biotechnological application of novel regulatory genes involved in Streptomyces polyketide overproduction through reverse engineering strategy. Biomed Res Int. 2013;2013:549737.
Lee HN, Kim HJ, Kim P, Lee HS, Kim ES. Minimal polyketide pathway expression in an actinorhodin cluster-deleted and regulation-stimulated Streptomyces coelicolor. J Ind Microbiol Biotechnol. 2012;39:805–11.
DK conceptualized the study, designed and performed the experiments, analyzed and interpreted the data, and wrote the manuscript. GJ performed the LC–MS analysis of the samples. HL and YD supervised the study and revised the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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Kallifidas, D., Jiang, G., Ding, Y. et al. Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters. Microb Cell Fact 17, 25 (2018). https://doi.org/10.1186/s12934-018-0874-2
- Secondary metabolism
- Streptomyces albus host engineering
- Heterologous expression
- Synthetic biology