Fig. 2

a Strategy for in-frame deletion of the wblA gene in S. albus using λ-mediated recombineering. b PCR confirmation of unmarked wblA deletion in different backgrounds. M, 1 kb ladder. Solid black arrows represent primers used for PCR screening and sizes of the PCR products are indicated. c S. albus ΔwblA mutant showed a sporulation-deficient phenotype on MS agar media