Fig. 5
a Paulomycin gene cluster knockout strategy. b PCR confirmation of 15 kb deletion from the paulomycin gene cluster in different backgrounds. M, 1 kb ladder. Solid black arrows represent primers used for PCR screening and sizes of the PCR products are indicated. Confirmation of upregulation of paulomycin gene cluster c in triple mutant S. albus ΔpfkΔwblA+ermE*crpSC versus S. albus ΔpfkΔwblAΔplm+ermE*crpSC by LC–MS profiling of culture extracts produced following 6 days of growth in R5A media and d antimicrobial assay of culture extracts derived from S. albus and engineered strains grown for 4 days in R5A media against Bacillus cereus. Minimum inhibition concentration for both S. albus+ermE*crpSC and ΔwblA derived extracts are reported to be 7.8 µg/ml