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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters

Fig. 1

a Strategy for in-frame deletion of pfk gene in S. albus. b PCR confirmation of pfk deletion; M, 1 kb ladder. Solid black arrows represent primers used for PCR screening and sizes of the PCR products are indicated. c Comparison of sensitivity to diamide (100 mM) between S. albus J1074 and the Δpfk derivative strain using diamide disc assays. The table shows the diameter and area of the halo formed around a disk impregnated with diamide. Values are means of three replicates. ± standard deviation (p < 0.01). Statistical significance was calculated with Student’s t-test

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