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Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast

Microbial Cell Factories volume 15, Article number: 198 (2016) Cite this article

Abstract

Background

BAHD acyltransferases, named after the first four biochemically characterized enzymes of the group, are plant-specific enzymes that catalyze the transfer of coenzyme A-activated donors onto various acceptor molecules. They are responsible for the synthesis in plants of a myriad of secondary metabolites, some of which are beneficial for humans either as therapeutics or as specialty chemicals such as flavors and fragrances. The production of pharmaceutical, nutraceutical and commodity chemicals using engineered microbes is an alternative, green route to energy-intensive chemical syntheses that consume petroleum-based precursors. However, identification of appropriate enzymes and validation of their functional expression in heterologous hosts is a prerequisite for the design and implementation of metabolic pathways in microbes for the synthesis of such target chemicals.

Results

For the synthesis of valuable metabolites in the yeast Saccharomyces cerevisiae, we selected BAHD acyltransferases based on their preferred donor and acceptor substrates. In particular, BAHDs that use hydroxycinnamoyl-CoAs and/or benzoyl-CoA as donors were targeted because a large number of molecules beneficial to humans belong to this family of hydroxycinnamate and benzoate conjugates. The selected BAHD coding sequences were synthesized and cloned individually on a vector containing the Arabidopsis gene At4CL5, which encodes a promiscuous 4-coumarate:CoA ligase active on hydroxycinnamates and benzoates. The various S. cerevisiae strains obtained for co-expression of At4CL5 with the different BAHDs effectively produced a wide array of valuable hydroxycinnamate and benzoate conjugates upon addition of adequate combinations of donors and acceptor molecules. In particular, we report here for the first time the production in yeast of rosmarinic acid and its derivatives, quinate hydroxycinnamate esters such as chlorogenic acid, and glycerol hydroxycinnamate esters. Similarly, we achieved for the first time the microbial production of polyamine hydroxycinnamate amides; monolignol, malate and fatty alcohol hydroxycinnamate esters; tropane alkaloids; and benzoate/caffeate alcohol esters. In some instances, the additional expression of Flavobacterium johnsoniae tyrosine ammonia-lyase (FjTAL) allowed the synthesis of p-coumarate conjugates and eliminated the need to supplement the culture media with 4-hydroxycinnamate.

Conclusion

We demonstrate in this study the effectiveness of expressing members of the plant BAHD acyltransferase family in yeast for the synthesis of numerous valuable hydroxycinnamate and benzoate conjugates.

Background

Chemical synthesis from non-renewable resources is the route most employed for production of chemicals used in both the pharmaceutical and flavor and fragrance industries. Several alternatives have emerged to limit the use of petroleum-based chemicals and to develop environmentally friendly methods through decreasing solvent utilization and reducing the carbon footprint of manufacturing processes. An example of such an alternative is biological synthesis via the use of engineered microbes for the production of fine and specialty chemicals. Because these processes consume renewable lignocellulosic biomass as a carbon source, they have reduced need for toxic chemicals and also offers consistent quality, scalability, simple extraction procedures and the potential for higher synthesis efficiency [1, 2]. In addition, biological synthesis could expand the chemical diversity of natural products, the structural complexity of which is sometimes challenging to achieve using multistep chemical synthesis [3]. In this area, the industrial microorganism S. cerevisiae is a powerful host platform for the biosynthesis of plant secondary metabolites such as beta-carotene, amorphadiene, valencene, casbene, cubebol, linalool, patchoulol, resveratrol and vanillin. This is due to its food-grade status, its advantages in the expression of complex metabolic pathways, extensive knowledge regarding its use in large-scale production, the availability of genetic tools, and its biodiversity [4].

BAHD acyltransferases are plant enzymes that catalyze the transfer of coenzyme A-activated donors—which include acetyl-CoA, β-phenylalanine-CoA, malonyl-CoA, tiglyl-CoA, anthraniloyl-CoA, benzoyl-CoA and (hydroxy)cinnamoyl-CoA—onto several types of acceptor molecules [5]. BAHDs catalyze O- or N-acylation of acceptors by acylating their hydroxyl (OH) or amine (NH2) groups, respectively, with CoA donors (Fig. 1). In plants, these enzymes participate in the synthesis of a wide range of polymers and secondary metabolites such as cutin, suberin, lignin, volatiles, pigments and defense compounds [5]. Furthermore, several BAHD acyltransferases catalyze the formation of metabolites beneficial for human health, which has prompted their use as biocatalysts in engineered microbes. For example, engineered Escherichia coli strains have been developed to express BAHDs such as hydroxycinnamoyl-CoA:quinate transferases (HQT) for the synthesis of the antioxidant chlorogenic acid [6, 7]; hydroxycinnamoyl-CoA:hydroxyphenyllactate transferases for the production of rosmarinic acid [8–10]; hydroxycinnamoyl-CoA:glycerol transferase for the synthesis of the water-soluble antioxidants hydroxycinnamate glycerol esters [11] and hydroxycinnamoyl/benzoyl-CoA:anthranilate transferase (HCBT) for the production of therapeutic benzoyl and hydroxycinnamoyl anthranilates [12]. To our knowledge, the use of yeast strains engineered for the expression of BAHD acyltransferases has not been reported, except for the synthesis of hydroxycinnamoyl anthranilates using either HCBT or promiscuous hydroxycinnamoyl-CoA:shikimate transferases [13–16].

Fig. 1
figure 1

Mechanism of acylation catalyzed by BAHD acyltransferases. Acyl donors are activated upon esterification of their carboxylic group (COOH) with coenzyme A (CoA). BAHD acyltransferases using 4-hydroxycinnamoyl-CoAs (R = 4-hydroxystyrene) and benzoyl-CoAs (R = benzene) as donors were used for this study. R’ = BAHD acceptor

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Hydroxycinnamic acids such as p-coumaric acid, caffeic acid, ferulic acid and sinapic acid possess antioxidant properties, antimicrobial activities and have been implicated in the prevention of cardiovascular diseases and cancer [17]. Importantly, the pharmacokinetics and bioavailability of hydroxycinnamic acids, as well as their antimicrobial activity, are highly dependent on their chemical structures and the types of molecules to which they are conjugated [17, 18]. Moreover, certain benzoate conjugates are also used for their health benefits and therapeutic effects [19, 20], whereas benzoate alcohol esters are widely used as flavoring agents in the food and cosmetic industries [21, 22].

In this study, we investigated the potential of 10 members of the BAHD acyltransferase family for the production of diverse hydroxycinnamate and benzoate conjugates in S. cerevisiae after feeding with compatible acceptor molecules. These enzymes were selected based on their capacity to use hydroxycinnamoyl-CoAs and/or benzoyl-CoAs as donor molecules and their affinity for structurally divergent acceptors. We have previously reported on a two-gene strategy for co-expression in S. cerevisiae of Arabidopsis (Arabidopsis thaliana) 4-coumarate:CoA ligase 5 (4CL5) and HCBT for the synthesis of hydroxycinnamoyl anthranilates upon feeding with hydroxycinnamates and anthranilate [13]. We also recently showed evidence that 4CL5 can accept benzoic acid as a substrate, which enabled the production of benzoyl anthranilates when co-expressed with HCBT [14]. Here, taking advantage of 4CL5 substrate’s promiscuity, several BAHD acyltransferases were selected and co-expressed with 4CL5 in an attempt to synthesize in yeast novel hydroxycinnamate and benzoate esters and amides. Furthermore, although the yields of product formation were typically two to fivefold lower compared to those achieved with p-coumarate feedings, additional expression of tyrosine ammonia-lyase from Flavobacterium johnsoniae (FjTAL), which converts tyrosine into p-coumarate [23], enabled the synthesis of p-coumarate esters and amides directly from the endogenous tyrosine pool.

Results and discussion

Synthesis of rosmarinic acid and analogues

Rosmarinic acid (RA), a caffeate ester of 3,4-dihydroxyphenyllactate, and its analogues, are strong antioxidants with several beneficial properties for human health [24]. The RA synthase LaAT1 from lavender (Lavandula angustifolia), a member of the BAHD family, was selected for expression in yeast because of its substrate promiscuity towards various donors and acceptors [25]. A S. cerevisiae strain co-expressing 4CL5 and LaAT1 was generated. Growing this strain in the presence of 4-hydroxyphenyllactate (LaAT1’s preferred acceptor), p-coumarate, caffeate and ferulate resulted in the production of p-coumaroyl 4′-hydroxyphenyllactate, caffeoyl 4′-hydroxyphenyllactate (isorinic acid) and feruloyl 4′-hydroxyphenyllactate, respectively (Fig. 2a–c). Similarly, feeding the strain with 3,4-dihydroxyphenyllactate as the acceptor and p-coumarate, caffeate and ferulate as donors resulted in the production of p-coumaroyl 3′,4′-dihydroxyphenyllactate, caffeoyl 3′,4′-dihydroxyphenyllactate (RA) and feruloyl 3′,4′-dihydroxyphenyllactate, respectively (Fig. 2d–f). Quantification of RA using an authentic standard showed that the culture medium contained 429 ± 81 µM (or 154 mg/L) (Fig. 2g). None of these products were observed in the culture medium of a control strain expressing 4CL5 alone and fed with the same precursors (data not shown). To our knowledge, this is the first report of RA biosynthesis in yeast. Moreover, considering the substrate promiscuity of LaAT1 [25], additional RA analogues would likely be produced by supplying the culture medium with alternative donors and acceptors. Such substrate flexibility has been explored for RA synthase from Coleus blumei and allowed biosynthesis of 13 RA analogues in E. coli [10]. Heterelogous pathways for the synthesis of the two acceptors, 4-hydroxyphenyllactate and 3,4-dihydroxyphenyllactate, from an inexpensive renewable carbon source has already been demonstrated in E. coli [8, 9] and could be implemented in yeast for sustainable and economical biosynthesis. We also demonstrated that expression of tyrosine ammonia-lyase (FjTAL) in addition to 4CL5 and LaAT1 results in the production of p-coumaroyl 4′-hydroxyphenyllactate when the FjTAL-4CL5-LaAT1 strain is fed with only 4-hydroxyphenyllactate (Additional file 1: Figure S1A). This result shows a potential in yeast for producing hydroxycinnamates more sustainably and directly by using endogenous tyrosine as a precursor pool.

Fig. 2
figure 2

Production of rosmarinic acid and analogues in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and LaAT1 are shown. The strain was fed with 4-hydroxyphenyllactate and p-coumarate, caffeate and ferulate for the synthesis of p-coumaroyl 4′-hydroxyphenyllactate (a), caffeoyl 4′-hydroxyphenyllactate (b) and feruloyl 4′-hydroxyphenyllactate (c), respectively. Feeding the strain with 3,4-dihydroxyphenyllactate and p-coumarate, caffeate and ferulate allowed the synthesis of p-coumaroyl 3′,4′-dihydroxyphenyllactate (d), rosmarinic acid (e) and feruloyl 3′,4′-dihydroxyphenyllactate (f), respectively. The LC–MS chromatogram of a solution of authentic rosmarinic acid is also shown (g)

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Synthesis of chlorogenic acid

Caffeoyl quinate (chlorogenic acid) and its derivatives have several beneficial properties for human health [6, 7, 26]. NtHQT, a BAHD enzyme from tobacco (Nicotiana tabacum), was selected for the synthesis of coumaroyl quinate and chlorogenic acid in S. cerevisiae because it uses both p-coumaroyl-CoA and caffeoyl-CoA as donors and quinate as its acceptor [27]. We confirmed that feeding a strain expressing 4CL5 and NtHQT with quinate and p-coumarate or caffeate resulted in the production of the two target metabolites, namely coumaroyl quinate and chlorogenic acid (Additional file 1: Figure S2). The subsequent quantification of chlorogenic acid using an authentic standard showed that the culture medium contained 1.43 ± 0.21 µM (or 506 µg/L). To our knowledge, this is the first example of chlorogenic acid biosynthesis in yeast. Because of the conservation of the shikimate pathway between bacteria and yeast, engineering strategies for accumulation of quinate described in E. coli [28] could be easily transferred to our 4CL5-NtHQT yeast strain to achieve chlorogenic acid synthesis without feeding quinate.

Synthesis of glycerol hydroxycinnamates

Glycerol hydroxycinnamate esters have higher water solubility than non-conjugated hydroxycinnamates and several of these esters, such as feruloyl glycerol conjugates, have antioxidant capacities and neuroprotective effects [29, 30]. Hydroxycinnamoyl-CoA:glycerol transferase OsHCT4 from rice (Oryza sativa) was selected for the synthesis of hydroxycinnamoyl glycerol conjugates in yeast [11]. Because OsHCT4 uses p-coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA as donors, a strain co-expressing 4CL5 and OsHCT4 was generated and fed with glycerol and either p-coumarate, ferulate or caffeate. For these cultures, analysis of the medium revealed the presence of p-coumaroyl glycerol, feruloyl glycerol and caffeoyl glycerol, respectively (Fig. 3a–c). Interestingly, and only in the case of caffeate feeding, a compound with a m/z value matching that of trisubstituted glycerol (i.e., 1,2,3-tricaffeoyl glycerol) was also detected (Fig. 3d). None of these products were observed in the culture medium of a strain expressing 4CL5 alone and fed with the same precursors (data not shown). These experiments are the first example of synthesis of hydroxycinnamate glycerol conjugates in yeast. Finally, using its endogenous glycerol pool, a strain expressing FjTAL, 4CL5 and OsHCT4 produced p-coumaroyl glycerol without the addition of any precursors to the culture medium (Additional file 1: Figure S1B).

Fig. 3
figure 3

Production of glycerol hydroxycinnamates in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and OsHCT4 are shown. The strain was fed with glycerol and p-coumarate, ferulate and caffeate for the synthesis of p-coumaroyl glycerol (a), feruloyl glycerol (b) and caffeoyl glycerol (c), respectively. Structures corresponding to 2-O-hydroxycinnamoyl-glycerides are represented as previously determined [11]. A compound with a mass matching that of 1,2,3-tricaffeoyl glycerol was also detected in the case of caffeate feedings (d)

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Synthesis of polyamine hydroxycinnamates

Multiple studies have reported on the beneficial properties of hydroxycinnamate amides of polyamines. For example, spermidine hydroxycinnamate conjugates have antioxidant [31, 32], antibacterial [33], antiviral [34], insecticidal [35] and antiparasitic [36] activities. Other studies have demonstrated the inhibitory activity of polysubstituted p-coumaroyl polyamines on brain transporters for which a genetic polymorphism is associated with psychological disorders [37, 38]. For this experiment, we selected the BAHDs p-coumaroyl-CoA:spermidine (AtSCT) and sinapoyl-CoA:spermidine (AtSDT) transferases from Arabidopsis [39], and p-coumaroyl-CoA:agmatine transferase (HvACT) from barley (Hordeum vulgare) [40]. Consistent with the properties of both AtSCT and AtSDT to catalyze the transfer of two hydroxycinnamate moieties onto spermidine [39], feeding with spermidine and p-coumarate or sinapate strains co-expressing 4CL5 with AtSCT or AtSDT resulted in the synthesis of dicoumaroyl and disinapoyl spermidine conjugates, respectively (Fig. 4a, b). In particular, a single new liquid chromatography-mass spectrometry (LC–MS) peak with a m/z value corresponding to 438.2395 [M + H]+ was detected in the medium of the 4CL5-AtSCT strain (Fig. 4a), suggesting the presence of N 1,N 8-dicoumaroyl spermidine as previously observed in vitro [39]. In the case of the 4CL5-AtSDT strain, LC–MS analysis of the culture medium showed a double LC–MS peak with a m/z value corresponding to 558.2808 [M + H]+ (Fig. 4b), suggesting the presence of another disinapoyl spermidine isomer (i.e. N 1,N 4-disinapoyl spermidine or N 4,N 8-disinapoyl spermidine) in addition to the previously characterized AtSDT product, N 1,N 8-disinapoyl spermidine [39]. The presence of a monosubstituted spermidine (i.e., p-coumaroyl spermidine) was also observed in the culture medium of the 4CL5-AtSCT strain but not in that of the 4CL5-AtSDT strain (Fig. 4c). Experiments exploring the substrate promiscuity of AtSDT and AtSCT were conducted to produce additional polyamine hydroxycinnamates. Feeding the 4CL5-AtSCT strain with spermidine and ferulate or sinapate successfully enabled the biosynthesis of feruloyl spermidine and sinapoyl spermidine, respectively (Fig. 4d, e). Moreover, a double LC–MS peak with a m/z value matching that of diferuloyl spermidine was detected when ferulate was supplied (Fig. 4f), but the exact identities of the two isomers was not determined. Next, feeding the 4CL5-AtSDT strain with sinapate and putrescine or spermine resulted in the synthesis of sinapoyl putrescine and disinapoyl spermine, respectively (Fig. 4g, h). These results show that the substrate flexibility of the two enzymes toward both donors and acceptors can be exploited for the synthesis of diverse polyamine hydroxycinnamates. This is the first report of biological synthesis of polyamine hydroxycinnamates in microbes, and our results show the potential for the production of disubstituted polyamine conjugates. We also demonstrated that co-expression of FjTAL with 4CL5 and AtSCT resulted in the production of N 1,N 8-dicoumaroyl spermidine without the addition of any precursors to the culture medium (Additional file 1: Figure S1C), providing a potential route to sustainable synthesis of such compounds. This indicates that spermidine pools in S. cerevisiae are sufficient for such biosynthetic approaches. In that respect, recently developed engineering strategies for enhancing spermidine content in yeast could be applied to our strain [41]. Lastly, our list of microbially produced polyamine hydroxycinnamates was extended after a strain co-expressing FjTAL, 4CL5 and HvACT and fed with agmatine was able to produce p-coumaroyl agmatine (Additional file 1: Figure S1D).

Fig. 4
figure 4

Production of polyamine hydroxycinnamates in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of S. cerevisiae strains expressing 4CL5 and AtSCT (a, c–f) or 4CL5 and AtSDT (b, g, h) are shown. The 4CL5-AtSCT strain was fed with spermidine and p-coumarate, ferulate or sinapate for the synthesis of dicoumaroyl spermidine (a) p-coumaroyl spermidine (c), feruloyl spermidine (d), sinapoyl spermidine (e) and diferuloyl spermidine (f). The 4CL5-AtSDT strain was fed with sinapate and spermidine, putrescine and spermine for the synthesis of disinapoyl spermidine (b) sinapoyl putrescine (g) and disinapoyl spermine (h), respectively. Structures in a and b are represented as previously determined in [39] whereas other structures are arbitrarily shown with substitutions of the polyamines on N 1, N 8 (c–g) and N 1, N 12 (h)

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Synthesis of monolignol hydroxycinnamates

Monolignol hydroxycinnamates belong to another class of hydroxycinnamate ester conjugates with beneficial pharmacological properties. For example, coniferyl ferulate has antibacterial activity, antioxidant and anti-Alzheimer properties, and it was also shown to have a potential role in reversing multidrug resistance mechanisms acquired by tumor cells [42–44]. Feruloyl-CoA:monolignol transferase (AsFMT) from Chinese angelica (Angelica sinensis) is a BAHD enzyme involved in the synthesis of coniferyl ferulate by coupling feruloyl-CoA to the monolignol coniferyl alcohol [45]. A S. cerevisiae strain that co-expressed 4CL5 with AsFMT was generated and grown in the presence of ferulate and coniferyl alcohol for the synthesis of coniferyl ferulate. Interestingly, using LC–MS analysis and an authentic standard for comparison, we observed that coniferyl ferulate was effectively produced by the yeast strain (Fig. 5a–b) but remained exclusively intracellular. This is the first successful attempt at producing coniferyl ferulate in microbes. To facilitate its purification and reduce production costs, it would be useful to evaluate other hosts for their capability to synthesize and excrete this valuable product. In this respect, several strains of E. coli have been engineered for the production of p-coumaryl alcohol via p-coumaroyl-CoA and could become suitable hosts [46, 47].

Fig. 5
figure 5

Production of coniferyl ferulate in yeast. A representative LC–MS chromatogram of a hexane extract of isolated S. cerevisiae cells expressing 4CL5 and AsFMT and previously grown in the presence of ferulate and coniferyl alcohol is shown (a). Identification of intracellular coniferyl ferulate was made by comparison with the LC–MS profile of a solution of authentic coniferyl ferulate (b)

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Synthesis of malate hydroxycinnamates

Malate hydroxycinnamates are another group of hydroxycinnamate esters with potential beneficial properties for human health, as exemplified by caffeoyl malate (phaselic acid), which possesses anti-inflammatory activities [48]. Hydroxycinnamoyl-CoA:malate transferase from red clover (Trifolium pratense) (TpHCT2) was selected for the production of malate hydroxycinnamates in yeast [49]. A S. cerevisiae strain co-expressing 4CL5 and TpHCT2 was fed with malate and p-coumarate or caffeate. LC–MS analysis of the culture medium showed the presence of peaks corresponding to p-coumaroyl malate and phaselic acid, respectively (Fig. 6a, b). These LC–MS peaks were absent from control cultures of a strain expressing 4CL5 alone and fed with the same precursors (data not shown). This is the first example of the production of malate hydroxycinnamates in microbes, and such synthesis could theoretically be achieved sustainably considering existing engineering methods used to obtain high titers of malate in yeast [50]. Concerning the de novo synthesis of hydroxycinnamate moieties, we also show that expression of FjTAL in the 4CL5-TpHCT2 strain can sustain the synthesis of p-coumaroyl malate without an external supply of p-coumarate (Additional file 1: Figure S1E).

Fig. 6
figure 6

Production of malate hydroxycinnamates in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and TpHCT2 are shown. The strain was fed with malate and p-coumarate or caffeate for the synthesis of p-coumaroyl malate (a) and phaselic acid (b), respectively

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Synthesis of alkyl hydroxycinnamates

Alkyl hydroxycinnamates such as hydroxycinnamate esters of fatty alcohols are lipophilic antioxidants that can efficiently inhibit the growth of tumor cells [51, 52]. Hydroxycinnamoyl-CoA:fatty alcohol transferase from Arabidopsis (AtHHT3), which accepts various medium chain fatty alcohols as substrates, was selected for the synthesis of dodecyl hydroxycinnamates [53]. In particular, a S. cerevisiae strain co-expressing 4CL5 with AtHHT3 and fed with 1-dodecanol and p-coumarate, caffeate or ferulate produced the potent antioxidants dodecyl p-coumarate, dodecyl caffetae and dodecyl ferulate, respectively (Fig. 7a–c). These alkyl hydroxycinnamates were not detected in cultures of a control strain expressing 4CL5 alone and fed with hydroxycinnamates and 1-dodecanol (data not shown). The microbial synthesis of alkyl hydroxycinnamates has never been reported and engineering strategies used for the production of fatty alcohols could be leveraged for their de novo synthesis [54, 55]. Moreover, using a strain that co-express FjTAL with 4CL5 and AtHHT3, we achieved the production of dodecyl p-coumarate by supplying only 1-dodecanol to the culture medium (Additional file 1: Fig. S1F).

Fig. 7
figure 7

Production of dodecyl hydroxycinnamates in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and AtHHT3 are shown. The strain was fed with 1-dodecanol and p-coumarate, caffeate or ferulate for the synthesis of dodecyl p-coumarate (a), dodecyl caffeate (b) and dodecyl ferulate (c), respectively

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Synthesis of cinnamoyl and benzoyl tropane analogues

Tropane alkaloids, such as cinnamate and benzoate esters of tropane skeletons, are of considerable interest due to their wide range of biological activities, and some are commonly employed in the pharmaceutical industry [56, 57]. Cocaine synthase (EcCS) from coca (Erythroxylum coca) catalyzes the transfer of both cinnamoyl-CoA and benzoyl-CoA onto 2-carbomethoxy-3β-tropine for the synthesis of the tropane alkaloids cinnamoylcocaine and cocaine, respectively [58]. Because we previously demonstrated the activity of 4CL5 towards cinnamate and benzoate in yeast [14], and considering that EcCS was also shown to use 3β-tropine as an acceptor in vitro [58], we generated a S. cerevisiae strain that co-express 4CL5 and EcCS for the synthesis of the tropane alkaloids cinnamoyl 3β-tropine and benzoyl 3β-tropine (tropacocaine). By comparison with an authentic standard, LC–MS analysis identified cinnamoyl 3β-tropine (9.58 ± 1.06 µM or 2.6 mg/L) in the culture medium of the 4CL5-EcCS strain grown in the presence of cinnamate and 3β-tropine (Fig. 8a, b). Similarly, benzoyl 3β-tropine was detected in the culture medium of the 4CL5-EcCS strain cultivated with the precursors benzoate and 3β-tropine (Fig. 8c). None of these products were detected in control cultures of a strain expressing 4CL5 alone and fed with the same precursors (data not shown). Nevertheless, further investigation will be needed since the detection of double LC–MS peaks strongly suggests the presence of two isomers for each product, possibly due to the isomerization of 3β-tropine in the culture medium. To our knowledge, this is the first report on the synthesis of tropane alkaloids in microbes, and it would be interesting to investigate the substrate flexibility of EcCS for the synthesis of pharmacologically valuable cinnamoyl and benzoyl tropane analogues [20]. As future work, the plant β-oxidative pathway of benzoic acid biosynthesis could be imported into yeast for the synthesis of benzoyl-CoA from phenylalanine [59], as well as the metabolic engineering strategies already established in hairy root culture systems for the synthesis of tropane moieties from arginine [60].

Fig. 8
figure 8

Production of tropane alkaloids in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and EcCS are shown. The strain was fed with 3β-tropine and cinnamate or benzoate for the synthesis of cinnamoyl 3β-tropine (a) and tropacocaine (c), respectively. Note the double LC–MS peaks suggesting the presence of two isomers for each product. The LC–MS chromatogram of a solution of authentic cinnamoyl 3β-tropine is also shown (b)

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Synthesis of benzoate alcohol esters and caffeate phenethyl ester (CAPE)

Benzoate alcohol esters are widely used as flavoring agents in foods and cosmetics as well as in hygiene and pharmaceutical products [21, 22]. Benzoyl-CoA:benzyl alcohol/phenylethanol benzoyltransferase (BPBT), a BAHD enzyme from petunia (Petunia x hybrida), uses benzoyl-CoA as a donor and various alcohols as acceptors for the formation of benzoate alcohol esters [61]. A S. cerevisiae strain was generated for the co-expression of 4CL5 and BPBT. Based on the substrate preference of BPBT observed in vitro [61], our 4CL5-BPBT strain was fed with benzoic acid in combination with ethanol, butanol, isopentanol or 2-phenylethanol. By comparison with the elution of authentic standards, LC–MS analysis of the culture media showed the presence of ethyl benzoate (1.39 ± 0.40 µM or 209 µg/L), butyl benzoate (2.65 ± 0.69 µM or 471 µg/L), isopentyl benzoate (2.50 ± 0.50 µM or 481 µg/L) and phenethyl benzoate (7.80 ± 1.73 µM or 1763 µg/L), respectively (Fig. 9a–h). Moreover, caffeate phenethyl ester (CAPE) is a bioactive compound that possesses antimicrobial, antioxidant, anti-inflammatory and cytotoxic properties [62, 63]. Therefore, the capacity of BPBT to accept 2-phenylethanol as a substrate is of particular interest toward the biological synthesis of CAPE and its derivatives, especially because its production using chemical synthesis or its purification from natural sources (e.g., honeybee hives) are low-yielding procedures [64]. Feeding our 4CL5-BPBT strain with caffeate and 2-phenylethanol resulted in successful synthesis of CAPE (1.61 ± 0.23 nM or 457 ng/L), which was identified by LC-MS analysis of the culture medium (Fig. 9i–j). Although several BAHDs acyltransferases similar to BPBT have been shown to transfer benzoyl-CoA onto 2-phenylethanol [65, 66], the use of hydoxycinnamoyl-CoA donors (e.g., caffeoyl-CoA) by these enzymes has never been reported and could enable the biological synthesis of valuable hydroxycinnamate phenethyl esters. Taking advantage of existing engineering strategies that yield high titers of alcohols and 2-phenylethanol should enable the economical synthesis of benzoate alcohol esters and CAPE in yeast [67, 68].

Fig. 9
figure 9

Production of benzoate alcohol esters and CAPE in yeast. Representative LC–MS chromatograms of hexane extracts of culture media of a S. cerevisiae strain expressing 4CL5 and BPBT are shown. The strain was fed with benzoate and ethanol, butanol, isopentanol or 2-phenylethanol for the synthesis of ethyl benzoate (a), butyl benzoate (c), isopentyl benzoate (e) and phenethyl benzoate (g), respectively. Identification of the different benzoate alcohol esters was made by comparison with the LC–MS profiles of authentic standards (b, d, f, h). Similarly, feeding the strain with caffeate and 2-phenylethanol allowed the synthesis of CAPE (i) by comparison with the LC–MS profile of an authentic CAPE standard (j)

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Conclusions

This study illustrates the potential of using BAHD acyltransferases from plants and S. cerevisiae as an expression host for the biological synthesis of valuable molecules that are usually produced from petrochemicals or found at very low concentration in plant extracts. In particular, expression of BAHD acyltransferases that use hydoxycinnamoyl-CoA and benzoyl-CoA allowed the synthesis of more than 30 different compounds which have applications across diverse sectors such as human health products and the flavor and fragrance industry. Exploring the substrate promiscuity of these BAHDs could considerably expand the number of molecules produced. Moreover, the use of BAHDs activity on alternate donors such as acetyl-CoA or malonyl-CoA should be explored for the biosynthesis of additional beneficial products.

Methods

Chemicals

The chemicals used for the S. cerevisiae feeding are listed in Additional file 1: Table S1. Rosmarinic acid was obtained from Spectrum Chemical Mfg. Corp. (New Brunswick, NJ), chlorogenic acid from Alfa Aesar (Haverhill, MA), ethyl benzoate, butyl benzoate, isopentyl benzoate and caffeate phenetyl ester from TCI America (Portland, OR), cinnamoyl 3β-tropine and phenethyl benzoate from Sigma-Aldrich (St. Louis, MO), and coniferyl ferulate from Biorbyt LLC (Berkeley, CA).

Strains, plasmids and media

The vectors pDRf1-4CL5-AtSDT, pDRf1-4CL5-AtSCT, pDRf1-4CL5-HvACT, pDRf1-4CL5-OsHCT4, pDRf1-4CL5-AsFMT, pDRf1-4CL5-EcCS, pDRf1-4CL5-TpHCT2, pDRf1-4CL5-LaAT1, pDRf1-4CL5-NtHQT, pDRf1-4CL5-AtHHT3, and pDRf1-4CL5-BPBT were used for co-expression of 4CL5 with the different BAHDs under the control of the constitutive promoters P HXT7 and P PMA1 , respectively (Table 1). The Gateway-enabled pRS423 vector was used for the expression of FjTAL under the control of the constitutive promoter P TDH3 . The S. cerevisiae pad1 knockout (MATa his3∆1 leu2∆0 met15∆0 ura3∆0 ∆pad1, ATCC 4005833) [69] was transformed using the Frozen-EZ Yeast Transformation II Kit™ (Zymo Research Corporation, Irvine, CA) and selected on solid medium containing yeast nitrogen base (YNB) without amino acids (Difco 291940; Difco, Detroit, MI) supplemented with 3% glucose and 1X dropout-uracil (CSM-ura) or 1X dropout-uracil-histidine (CSM-ura-his) (Sunrise Science Products, San Diego, CA).

Table 1 Plasmids used in this study
Full size table

Construction of plasmids

A description of the plasmids used in this study is provided in Table 1. For the construction of the pDRf1-4CL5-DsRed vector, a red fluorescent protein (DsRed) dropout cassette sequence (kindly provided by Chris Anderson’s lab, UC Berkeley) was synthesized with flanking sequences (Genescript, Piscataway, NJ; Additional file 1: Data S1). It contains at the 5′-end the 18-bp sequence located upstream of the EcoRI site in pDRf1-4CL5-GW [13], followed by an EcoRI restriction site, the Gateway recombination site attB1 and a PmlI restriction site. At the 3′-end, it contains a PmlI restriction site, followed by the Gateway recombination site attB2, a PstI restriction site and the 15-bp sequence located upstream of the PstI site in pDRf1-4CL5-GW. This sequence was amplified by PCR using the oligonucleotides DsRed-dropout-fw and DsRed-dropout-rv (Additional file 1: Table S2) and inserted by In-Fusion cloning (Clonetech, Mountain View, CA) into a pDRf1-4CL5-GW vector digested with EcoRI and PstI to remove the Gateway cassette and yield the pDRf1-4CL5-DsRed vector. BAHD acyltransferase nucleotide sequences were codon-optimized for expression in S. cerevisiae using an empirically derived codon usage table. Codon optimization, including restriction site removal, and oligo design (150 mers) were performed using GeneDesign [70]. Oligonucleotides were pooled for synthesis using acoustic deposition (Labcyte Echo 550), and synthesis was performed at the Joint Genome Institute using a two-step polymerase chain assembly (PCA) approach in 2 μL final volume as previously described [71]. PCA products were purified by gel excision and cloned into a PmlI-digested pDRf1-4CL5-DsRed vector using Gibson assembly to generate the pDRf1-4CL5-BAHDs vectors. Plating and picking were performed using a QPix 400 system (Molecular Devices, Sunnyvale, CA). Eight colonies per construct were sequence-verified using PACBIO RSII system (Pacific Biosciences, Menlo Park, CA). Synthesized BAHD sequences and protein accession numbers are listed in Additional file 1: Data S1.

For the construct of the pRS423-GW vector, two DNA fragments covering the full backbone including P TDH3 and T CYC1 sequences while excluding XKS1 encoding sequence of the pRS423.XI vector were amplified by PCR using the oligonucleotide pairs pRS423-fw1/pRS423-rv1 and pRS423-fw2/pRS423-rv2 (Additional file 1: Table S2) and the pRS423.XI vector as a template [72]. A third fragment corresponding to the Gateway cassette was amplified using the oligonucleotides GW-fw/GW-rv (Additional file 1: Table S2) and the pDRf1-4CL5-GW vector as a template [13]. The pRS423-GW vector was constructed by assembling the three PCR fragments via In-Fusion cloning (Clonetech, Mountain View, CA). For the construct of the pRS423-FjTAL vector, a nucleotide sequence encoding FjTAL (GenBank accession number AKE50827.1) flanked with the attB1 (5′-end) and attB2 (3′-end) Gateway recombination sites was synthesized for expression in S. cerevisiae (Genescript, Piscataway, NJ) and cloned into the Gateway pDONR221 entry vector by BP recombination (Life technologies, Foster City, CA). An entry clone was LR recombined with the pRS423-GW vector to generate the pRS423-FjTAL construct. Plasmids are available upon request through the JBEI-ICE registry (http://public-registry.jbei.org) [73].

Production of hydroxycinnamate and benzoate conjugates

Overnight cultures from single colonies of recombinant S. cerevisiae harboring the pDRf1-4CL5-BAHDs and pDRf1-4CL5 [13] constructs were grown using 2X YNB medium without amino acids, supplemented with 6% glucose and 2X CSM-ura. These cultures were used to inoculated 4 mL of fresh minimal medium at an OD600 = 0.15 and shaken at 200 rpm at 30 °C. Precursors were added 5 h post inoculation at the concentrations indicated in Additional file 1: Table S1. These concentrations were selected to be below toxicity levels and avoid growth inhibition. The cultures were shaken at 200 rpm at 30 °C for 24 h in the presence of the precursors for the production of hydrocinnamate and benzoate conjugates. Yeast colonies harboring the pDRf1-4CL5 control vector were grown under similar conditions. Yeast colonies co-transformed with the pRS423-FjTAL plasmid and the pDRf1-4CL5-LaAT1, pDRf1-4CL5-OsHCT4, pDRf1-4CL5-AtSCT, pDRf1-4CL5-HvACT, pDRf1-4CL5-TpHCT2, or pDRf1-4CL5-AtHHT3 vector were grown under similar conditions except that the medium was supplemented with 2X CSM-ura-his. For the detection of metabolites, an aliquot of the culture medium was collected and cleared by centrifugation (21,000×g for 5 min at 4 °C), mixed with an equal volume of cold methanol:water (1:1, v/v), and filtered using Amicon Ultra centrifugal filters (3000 Da MW cutoff regenerated cellulose membrane; Millipore, Billerica, MA) prior to analysis using high-performance liquid chromatography (HPLC), electrospray ionization (ESI), and time-of-flight (TOF) mass spectrometry (MS). Alternatively, for the detection of dodecyl hydroxycinnamates, caffeate phenethyl ester and benzoate alcohol esters, the culture medium was cleared by centrifugation and mixed with 2 mL of hexane for extraction with vortexing. The hexane phase was collected after centrifugation and concentrated under a nitrogen stream prior to HPLC-TOF-MS analysis. For the detection of coniferyl ferulate, yeast cells were harvested by centrifugation, washed and resuspended with 1 mL of HPLC grade water, and mixed with 2 mL of hexane for extraction with vortexing. The hexane phase was collected after centrifugation and concentrated under a nitrogen stream prior to HPLC-ESI-TOF-MS analysis.

LC–MS analysis of hydroxycinnamate and benzoate conjugates

Standard solutions of chlorogenic acid, rosmarinic acid, coniferyl ferulate and cinnamoyl 3β-tropine were prepared in methanol and water (1:1, v/v) and those of caffeate phenethyl ester and benzoate alcohol esters were prepared in hexane. All metabolites were separated via an Agilent 1200 Series Rapid Resolution high performance liquid chromatography (HPLC) system. The systems autosampler tray was set to 6 °C. The HPLC system was coupled to an Agilent Technologies 6210 LC/TOF mass spectrometer (MS) via either electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI), which was used for MS detection and metabolite identification. Drying gas temperature, drying gas pressure, nebulizing gas pressure and capillary voltage were set to 330 °C, 10 or 11 L/min, 25 lb/in2, and 3500 V (in positive or negative ion modes), respectively, unless stated otherwise. For APCI, the vaporizer and corona were set to 350 °C and 4 µA, respectively, unless stated otherwise.

A HPLC-ESI-TOF-MS method previously described in [12] was used for the separation and detection of rosmarinic acid and its derivatives, coumaroyl quinate and malate hydroxycinnamate esters. The same method was used for coniferyl ferulate except that the drying gas temperature was set at 200 °C. A similar method was used for glycerol hydroxycinnamate esters with the following modifications: The mobile phase was composed of water (solvent A) and methanol (solvent B), and metabolites were separated via gradient elution under the following mobile phase compositions: % B was linearly increased from 30% B to 98% B in 12 min, held at 98% B for 0.6 min, decreased from 98% B to 30% B in 0.2 min and held at 30% B for a further 2.8 min. The total run time was 15.6 min. A drying gas temperature of 200 °C was used throughout. A HPLC-ESI-TOF-MS method previously described in [74] was used for the separation and detection of polyamine hydroxycinnamates amides and tropane alkaloids.

Separation of benzoate alcohol esters was conducted on a Phenomenex Kinetex XB-C18 column (100 mm length, 2.1 mm internal diameter, and 2.6 µm particle size; Phenomenex, Torrance, CA). The mobile phase was composed of water (solvent A) and methanol (solvent B). The elution gradient was as follows: % B was linearly increased from 60% B to 100% B in 5.0 min, held at 100% B for 2.0 min, decreased from 100% B to 60% B in 0.2 min and held at 60% B for a further 4.5 min. A flow rate of 0.3 mL/min was used until 7.0 min, increased from 0.3 to 0.45 mL/min in 0.2 min and held at 0.45 mL/min for 4.5 min. The total run time was 11.7 min. The column compartment was set to 55 °C. A sample injection volume of 2 µL was used throughout. APCI was conducted using in the positive ion mode for the detection of [M + H]+ ions. The same HPLC column and column compartment temperature were used for the separation of CAPE. The mobile phase was composed of water (solvent A) and acetonitrile (solvent B) and the elution gradient was as follows: % B was increased linearly from 25% B to 100% B in 3.0 min, held at 100% B for 1.0 min, decreased from 100% B to 25% B in 0.2 min and held at 25% B for a further 4.0 min. A flow rate of 0.3 mL/min was used until 4.0 min, increased from 0.3 to 0.45 mL/min in 0.2 min and held at 0.45 mL/min for the remaining 4.0 min. The total run time was 8.2 min. A sample injection volume of 0.5 µL was used throughout. ESI was conducted in the negative ion mode for the detection of [M–H]− ions.

Separation of dodecyl hydroxycinnamates was conducted on a Phenomenex Kinetex XB-C18 column (100 mm length, 3.0 mm internal diameter and 2.6 μm particle size). A sample injection volume of 3 μL was used throughout. The column compartment was set to 55 °C. The mobile phase was composed of water (solvent A) and methanol (solvent B). The elution gradient was as follows: % B was increased linearly from 65% B to 98% B in 1.47 min, held at 98% B for 9.2 min, decreased from 98% B to 65% B in 0.33 min and held at 65% B for a further 2.0 min. A flow rate of 0.42 mL/min was used until 10.67 min, increased from 0.42 to 0.65 mL/min in 0.33 min and held at 0.65 mL/min for 2.0 min. The total run time was 13.0 min. APCI was conducted in the positive ion mode for the detection of [M + H]+ ions. The corona and capillary voltage were set to 15 µA and 3000 V, respectively. A nebulizing gas pressure of 30 lb/in2 and a drying gas temperature of 200 °C were used throughout.

A HPLC-ESI TOF-MS method was used to detect chlorogenic acid. The chromatography was done using a Phenomenex Kinetex XB-C18 column (100 mm length, 3 mm internal diameter and 2.6 µm particle size) using the aforementioned HPLC system. A sample injection volume of 5 μL was used throughout. The mobile phase was composed of 0.1% formic acid in water (solvent A) and in methanol (solvent B). The elution gradient was as follows: % B was increased linearly from 25 to 77.5% B in 3.0 min, then increased from 77.5 to 97.1% B in 0.3 min, held at 97.1% B for 1.0 min, decreased from 97.1 to 25% B in 0.4 min and held at 25% B for a further 2 min. A flow rate of 0.42 mL/min was used until 4.3 min, then increased from 0.42 to 0.65 mL/min in 0.4 min and held at 0.65 mL/min for 2 min. The total run time was 6.7 min. The column compartment and sample tray were set to 50 °C and 6 °C, respectively. A sample injection volume of 3 µL was used throughout. ESI was conducted in the negative ion mode for the detection of [M–H]− ions. The nebulizing gas pressure was set to 30 lb/in2.

Data acquisition and processing were performed by the MassHunter software package (Agilent Technologies Inc., Santa Clara, CA). For each compound detected, the measured masses agreed with the expected theoretical masses within less than 5 ppm mass error.

Abbreviations

AsFMT:

Angelica sinensis feruloyl-CoA:monolignol transferase

AtHHT3:

Arabidopsis thaliana caffeoyl-CoA:fatty alcohol transferase

AtSCT:

Arabidopsis thaliana p-coumaroyl-CoA:spermidine transferase

AtSDT:

Arabidopsis thaliana sinapoyl-CoA:spermidine transferase

At4CL5:

Arabidopsis thaliana 4-coumarate:CoA ligase 5

BAHD:

BEAT (benzoyl alcohol O-acetyltransferase), AHCT (anthocyanin O-hydroxycinnamoyl transferase), HCBT (anthranilate N-hydroxycinnamoyl/benzoyl transferase), and DAT (deacetyl vindoline 4-O-acetyltransferase)

BPBT:

benzoyl-CoA:benzyl alcohol/phenylethanol benzoyltransferase

CAPE:

caffeate phenethyl ester

CoA:

coenzyme A

EcCS:

Erythroxylum coca cocaine synthase

FjTAL:

Flavobacterium johnsoniae tyrosine ammonia-lyase

HCBT:

hydroxycinnamoyl/benzoyl-CoA:anthranilate transferase

HvACT:

Hordeum vulgare p-coumaroyl-CoA:agmatine transferase

LC–MS:

liquid chromatography-mass spectrometry

NtHQT:

Nicotiana tabacum hydroxycinnamoyl-CoA:quinate transferase

OsHCT4:

Oryza sativa hydroxycinnamoyl-CoA:shikimate transferase 4

RA:

rosmarinic acid

TpHCT2:

Trifolium pratense hydroxycinnamoyl-CoA:shikimate transferase 2

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Authors’ contributions

AE wrote the manuscript. DL edited the document. AE, LR, MM, VTB and Y-LT performed the experiments. T-YC generated the p423-GW yeast vector. EEKB and GW conducted the LC–MS analyses. DR and SD synthesized the genes and assembled them in the yeast expression vector. AE, AM, HVS, JDK, SD and DL supervised the research. AM, DL, HVS, JDK, JLH and SD provided chemicals and financial support. All authors read and approved the final manuscript.

Acknowledgements

We thank Sabin Russell for editing this manuscript.

Competing interests

JDK has financial conflicts of interest in Amyris and Lygos. DL and HVS have financial conflicts of interest in Afingen Inc.

Availability of data and materials

All data generated or analyzed during this study are included in this published article and its supplementary information files. Plasmids are available upon request through the JBEI-ICE registry (http://public-registry.jbei.org).

Funding

This work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research and the US Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes.

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Authors and Affiliations

  1. Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St., 4th Floor, Emeryville, CA, 94608, USA

    Aymerick Eudes, Maxence Mouille, Veronica T. Benites, George Wang, Lucien Roux, Yi-Lin Tsai, Edward E. K. Baidoo, Tsan-Yu Chiu, Joshua L. Heazlewood, Henrik V. Scheller, Aindrila Mukhopadhyay, Jay D. Keasling & Dominique Loqué

  2. Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA

    Aymerick Eudes, Maxence Mouille, Veronica T. Benites, George Wang, Lucien Roux, Yi-Lin Tsai, Edward E. K. Baidoo, Tsan-Yu Chiu, Joshua L. Heazlewood, Henrik V. Scheller, Aindrila Mukhopadhyay, Jay D. Keasling & Dominique Loqué

  3. Joint Genome Institute, Walnut Creek, CA, 94598, USA

    David S. Robinson & Samuel Deutsch

  4. Graduate Program, San Francisco State University, San Francisco, CA, 94132, USA

    Veronica T. Benites

  5. Master Program, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland

    Lucien Roux

  6. School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia

    Joshua L. Heazlewood

  7. Department of Chemical & Biomolecular Engineering and Department of Bioengineering, University of California, Berkeley, CA, 94720, USA

    Jay D. Keasling

  8. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle´, 2970, Hørsholm, Denmark

    Jay D. Keasling

  9. CNRS, UMR5240, Microbiologie, Adaptation et Pathogénie, Université Claude Bernard Lyon 1, INSA de Lyon, 10 rue Raphaël Dubois, 69622, Villeurbanne, France

    Dominique Loqué

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  1. Aymerick Eudes
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Additional file

12934_2016_593_MOESM1_ESM.pdf

Additional file 1: Figure S1. Synthesis of p-coumarate esters and amides from tyrosine in yeast. S. cerevisiae strains co-expressing tyrosine ammonia-lyase FjTAL and 4CL5 with LaAT1 (A), OsHCT4 (B), AtSCT (C), HvACT (D), TpHCT2 (E) or AtHHT3 (F) were fed with 4-hydroxyphenyllactate (A), nothing (B, C), agmatine (D), malate (E) or 1-dodecanol (F) for the synthesis of p-coumaroyl 4’-hydroxyphenyllactate (A), p-coumaroyl glycerol (B), N 1,N 8-disinapoyl spermidine (C), p-coumaroyl agmatine (D), p-coumaroyl malate (E) and dodecyl p-coumarate (F), respectively. Figure S2. Synthesis of quinate hydroxycinnamates in yeast. Representative LC-MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and NtHQT are shown. The strain was fed with quinate and p-coumarate or caffeate for the synthesis of p-coumaroyl quinate (A) and chlorogenic acid (B), respectively. The LC-MS chromatogram of a solution of authentic chlorogenic acid is also shown (C). Table S1. List of acyl acceptors and donors used for the feedings of yeast strains expressing 4CL5 and BAHDs. Concentrations used in the culture medium are indicated. Table S2. Oligonucleotides used in this study. Data S1. Sequence of the red fluorescent protein dropout cassette and the codon-optimized sequences and accession numbers of BAHD acyltransferases used in this study.

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Eudes, A., Mouille, M., Robinson, D.S. et al. Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast. Microb Cell Fact 15, 198 (2016). https://doi.org/10.1186/s12934-016-0593-5

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Keywords

Microbial Cell Factories

ISSN: 1475-2859

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