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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast

Fig. 8

Production of tropane alkaloids in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and EcCS are shown. The strain was fed with 3β-tropine and cinnamate or benzoate for the synthesis of cinnamoyl 3β-tropine (a) and tropacocaine (c), respectively. Note the double LC–MS peaks suggesting the presence of two isomers for each product. The LC–MS chromatogram of a solution of authentic cinnamoyl 3β-tropine is also shown (b)

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Microbial Cell Factories

ISSN: 1475-2859

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