Fig. 9

Production of benzoate alcohol esters and CAPE in yeast. Representative LC–MS chromatograms of hexane extracts of culture media of a S. cerevisiae strain expressing 4CL5 and BPBT are shown. The strain was fed with benzoate and ethanol, butanol, isopentanol or 2-phenylethanol for the synthesis of ethyl benzoate (a), butyl benzoate (c), isopentyl benzoate (e) and phenethyl benzoate (g), respectively. Identification of the different benzoate alcohol esters was made by comparison with the LC–MS profiles of authentic standards (b, d, f, h). Similarly, feeding the strain with caffeate and 2-phenylethanol allowed the synthesis of CAPE (i) by comparison with the LC–MS profile of an authentic CAPE standard (j)