Fig. 3

Production of glycerol hydroxycinnamates in yeast. Representative LC–MS chromatograms obtained from analysis of the culture medium of a S. cerevisiae strain expressing 4CL5 and OsHCT4 are shown. The strain was fed with glycerol and p-coumarate, ferulate and caffeate for the synthesis of p-coumaroyl glycerol (a), feruloyl glycerol (b) and caffeoyl glycerol (c), respectively. Structures corresponding to 2-O-hydroxycinnamoyl-glycerides are represented as previously determined [11]. A compound with a mass matching that of 1,2,3-tricaffeoyl glycerol was also detected in the case of caffeate feedings (d)