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Enzymatic fine-tuning for 2-(6-hydroxynaphthyl) β-d-xylopyranoside synthesis catalyzed by the recombinant β-xylosidase BxTW1 from Talaromyces amestolkiae
Microbial Cell Factories volume 15, Article number: 171 (2016)
Glycosides are compounds displaying crucial biological roles and plenty of applications. Traditionally, these molecules have been chemically obtained, but its efficient production is limited by the lack of regio- and stereo-selectivity of the chemical synthesis. As an interesting alternative, glycosidases are able to catalyze the formation of glycosides in a process considered green and highly selective. In this study, we report the expression and characterization of a fungal β-xylosidase in Pichia pastoris. The transglycosylation potential of the enzyme was evaluated and its applicability in the synthesis of a selective anti-proliferative compound demonstrated.
The β-xylosidase BxTW1 from the ascomycete fungus Talaromyces amestolkiae was cloned and expressed in Pichia pastoris GS115. The yeast secreted 8 U/mL of β-xylosidase that was purified by a single step of cation-exchange chromatography. rBxTW1 in its active form is an N-glycosylated dimer of about 200 kDa. The enzyme was biochemically characterized displaying a K m and k cat against p-nitrophenyl-β-d-xylopyranoside of 0.20 mM and 69.3 s−1 respectively, and its maximal activity was achieved at pH 3 and 60 °C. The glycan component of rBxTW1 was also analyzed in order to interpret the observed loss of stability and maximum velocity when compared with the native enzyme. A rapid screening of aglycone specificity was performed, revealing a remarkable high number of potential transxylosylation acceptors for rBxTW1. Based on this analysis, the enzyme was successfully tested in the synthesis of 2-(6-hydroxynaphthyl) β-d-xylopyranoside, a well-known selective anti-proliferative compound, enzymatically obtained for the first time. The application of response surface methodology, following a Box-Behnken design, enhanced this production by eightfold, fitting the reaction conditions into a multiparametric model. The naphthyl derivative was purified and its identity confirmed by NMR.
A β-xylosidase from T. amestolkiae was produced in P. pastoris and purified. The final yields were much higher than those attained for the native protein, although some loss of stability and maximum velocity was observed. rBxTW1 displayed remarkable acceptor versatility in transxylosylation, catalyzing the synthesis of a selective antiproliferative compound, 2-(6-hydroxynaphthyl) β-d-xylopyranoside. These results evidence the interest of rBxTW1 for transxylosylation of relevant products with biotechnological interest.
β-Xylosidases (EC 220.127.116.11) together with endo-β-1,4-xylanases (EC 18.104.22.168) play a central role in the complete hydrolysis of xylans, the most abundant hemicelluloses in nature. During the past 20 years, β-xylosidases have been largely studied for their potential application in second-generation bioethanol production , as they improve the effectiveness of commercial enzymatic cocktails, usually with poor β-xylosidase activity . However, these catalysts are interesting not only for their hydrolytic capacities, but also because many of these enzymes are able to catalyze transglycosylation reactions for glycoconjugate synthesis [3, 4].
The class of enzymes that is useful in this regard are the retaining glycosidases, which catalyze hydrolysis with net retention of anomeric configuration. Such enzymes operate through a double-displacement mechanism involving the formation of a covalent glycosyl-enzyme intermediate, which is subsequently cleaved upon nucleophilic attack by water. Transglycosylation occurs when some other nucleophile than water takes part, giving rise to a new glycoside . Transxylosylation is the particular name given to the reaction where a retaining β-xylosidase catalyzes the transfer of a xylose residue from a xylosyl donor, such as xylobiose, to an acceptor (Fig. 1).
The efficient synthesis of glycosides remains a significant challenge, especially for reactions that must be done on a large scale. Glycosides play important roles in biology. For example, the glycan moiety of glycoproteins and glycolipids is essential for many physiological processes, such as immune responses, cell adhesion or protein folding [5–7]. In addition, non-natural glycosides have been synthesized for a wide variety of applications, for example to enhance the properties of antioxidants or to generate new antibiotics [8, 9].
Chemical approaches to glycoconjugates synthesis typically require many protection and de-protection steps, in order to avoid the lack of regio- and stereo-selectivity displayed by chemical catalysts . Currently, the use of glycosidases for synthesis of glycosides appears as one of the most interesting enzymatic alternatives. Under kinetically controlled conditions, these enzymes can glycosylate certain acceptors, with high selectivity and sensible yields, in a process considered green . Unlike glycosyltransferases, which require high-cost nucleotide sugars as donors , glycosidases can use common and inexpensive glycans such as sucrose or starch, as sources of sugar donors [11, 12]. In the case of β-xylosidases, the use of xylobiose or other xylooligosaccharides (XOS), derived from the hydrolysis of xylan, may be a cost-effective alternative . The attachment of a xylose moiety to a specific acceptor can result, for instance, in novel surfactants , antithrombotic drugs  or primers for studying the biosynthesis of heparan sulfate . In fact, this priming role in the formation of glycosaminoglycans led to the development of specific xylosides as anti-proliferative compounds [17, 18], which have been successfully tested as selective inhibitors of the growth of tumor cells both in vitro and in vivo assays, as is the case for 2-(6-hydroxynaphthyl) β-d-xylopyranoside .
The purification and characterization of the BxTW1 β-xylosidase from Talaromyces amestolkiae has been recently reported. The enzyme, which belongs to the GH3 family (CAZy; http://www.cazy.org/), displays remarkable affinity and activity against xylobiose and XOS but, most importantly, it shows broad acceptor versatility and high regioselectivity in catalyzing transxylosylation reactions . However, the amount of protein secreted by the native producer was quite low, which, when coupled with the poor purification yields reported severely limited its potential application. In this work, we report the expression of high levels of the recombinant β-xylosidase (rBxTW1) in the methylotrophic yeast Pichia pastoris. In addition, we describe the rBxTW1-catalyzed synthesis of 2-(6-hydroxynaphthyl) β-d-xylopyranoside using xylobiose as xylosyl donor and 2,6-dihydroxynaphthalene (2,6-DHN) as acceptor. To the best of our knowledge, this is the first report on enzymatic synthesis of this xyloside, which is known as a selective anti-proliferative compound . The optimization of the reaction parameters by a Box-Behnken design (BBD) is also presented .
Enzyme and protein assays
β-Xylosidase activity was measured spectrophotometrically by the release of p-nitrophenol (pNP) (ε410 = 15,200 M−1 cm−1) from p-nitrophenyl β-d-xylopyranoside (pNPX) (Sigma-Aldrich). The standard assay was performed as described in Nieto-Domínguez et al. , but using sodium acetate buffer 50 mM (pH 5) instead of sodium citrate buffer. One unit of β-xylosidase activity was defined as the amount of enzyme that hydrolyzes 1 μmol of pNPX per minute in standard conditions.
The measurement of the β-xylosidase activity with xylobiose, xylooligosaccharides and beechwood xylan was performed by direct quantification of the released xylose. The d-xylose assay kit (Megazyme) was used for this purpose in accordance with the manufacturer’s instructions. In this case one unit of activity against xylobiose and XOS was defined as the amount of enzyme necessary for the complete hydrolysis of 1 μmol of the selected XOS to xylose per minute. Because of its polymeric nature, one unit of activity against xylan was considered to be the amount of enzyme necessary for releasing 1 μmol of xylose per minute.
Proteins were quantified by the bicinchoninic acid method by using the Pierce™ Protein Assay Kit (Thermo Scientific), according to the manufacturer’s instructions with bovine serum albumin as the standard.
Isolation of fungal genomic DNA and cloning of the β-xylosidase gene
The selected T. amestolkiae strain is deposited in the IJFM (Instituto “Jaime Ferrán” de Microbiología) culture collection of the Centro de Investigaciones Biológicas, CSIC (Madrid, Spain) with the reference designation A795.
Genomic DNA was isolated from the fungus by using the DNeasy Plant Mini Kit in accordance with the manufacturer’s instructions. Primers were designed based on the nucleotide sequence of the bxtw1 gene coding for BxTW1 from T. amestolkiae (GenBank accession no. KP119719), but excluding the region corresponding to the signal peptide, which was predicted by the SignalP 4.1 server (http://www.cbs.dtu.dk/services/SignalP/). Restriction sites for EcoRI and NotI were respectively added to the 5′ and 3′ primers (BxTw1 Fw, 5′-GAATTCCAGAACAACCAGACCTATGCCAATTACTCC-3′, and BxTw1 Rv, 5′-GCGGCCGCTTAATTGGGATCAGGTTGAATCTCCTGCTC-3′). The bxtw1 gene was amplified by PCR using the genomic DNA as template. PCR mixtures contained 100 ng of DNA template, 1 μL PCR buffer, 1.5 mM MgCl2, 0.8 mM deoxynucleoside triphosphates, 0.5 μM each primer, and 1 U of Expand™ High Fidelity PCR System DNA polymerase (Sigma-Aldrich) in a final volume of 50 μL. Reaction mixtures were denatured at 94 °C for 5 min and then subjected to 34 cycles of amplification, each at 94 °C for 45 s, 55 °C for 45 s, and 72 °C for 2.5 min, followed by a final extension step at 72 °C for 7 min. Control reactions lacking DNA template were simultaneously performed.
The PCR product, encoding BxTW1 without its signal peptide, was ligated to the yeast expression vector pPIC9 (Invitrogen), fused with the α-factor signal sequence and expressed under the transcriptional control of the methanol-inducible AOX1 promoter. The pPIC9:bxtw1 construct was named pPICW1N and used for transforming P. pastoris KM71 and GS115 after linearization with SacI (New England Biolabs). Transformed colonies were grown on Yeast Nitrogen Base plates in the absence of histidine as selection marker.
Screening for high-production clones
Transformed clones were screened for maximal production of recombinant BxTW1 (rBxTW1). The screening was performed on 25 clones from each strain. The colonies were cultured in a 96-well plate with 100 μL of YEPS medium (10 g/L yeast extract, 20 g/L peptone, 10 g/L sorbitol, 100 mM potassium phosphate buffer pH 6) per well. The medium included 5 g/L of methanol as inducer of gene expression. Cultures were incubated at 28 °C and 250 rpm and 50 μL of YEPS with 5 g/L of methanol were added at 24 and 48 h. Controls with both non-transformed P. pastoris KM71 and GS115 strains were included.
After 72 h the plate was centrifuged at 2000g for 15 min at 4 °C and 50 μL of each supernatant was placed into a new plate and incubated with 50 μL of a substrate solution containing 7 mM pNPX, 100 mM sodium formate buffer (pH 3) and 0.2 % BSA. The incubation was carried out at 50 °C for 10 min with gentle agitation. The reactions were stopped by the addition of 100 μL of 2 % Na2CO3 and the release of pNP was measured at 410 nm in a plate reader (SPECTRAMax Plus 384, Molecular Devices).
The two clones with highest activities from each strain were selected and liquid cultures were carried out as described below in order to compare clones in terms of secreted β-xylosidase activity.
Production of rBxTW1
To prepare a fresh inoculum, the selected clones were grown overnight in 250-mL flasks with 50 mL of YEPS medium at 28 °C and 250 rpm. Then, rBxTW1 production was carried out in 1-L flasks with 100 mL of YEPS medium and 3.5 mL of the inoculum. Cultures were incubated at 28 °C and 250 rpm for 10 days with daily addition of 5 g/L methanol. Samples were periodically withdrawn as described above to measure β-xylosidase activity and absorbance at 600 nm (A600) as estimation of yeast growth.
Purification of enzymes
For rBxTW1 purification, 10-day-old cultures were harvested and centrifuged at 10,000g and 4 °C for 20 min. The supernatant was sequentially filtered through 0.8-, 0.45- and 0.22-μm disc filters (Merck-Millipore). Then, the crude was first concentrated by tangential filtration and finally concentrated and dialyzed against 10 mM acetate buffer (pH 4) using a 50 kDa cutoff membrane (Merck-Millipore). rBxTW1 was purified by fast protein liquid chromatography (FPLC) using an ÄKTA Purifier chromatography system (GE Healthcare). The system was equilibrated in 10 mM sodium acetate buffer (pH 4) and the enzymatic crude was loaded onto a 5-mL Hi-Trap SPFF cartridge (GE Healthcare). The elution of the bound proteins was carried out by applying a linear gradient of 1 M NaCl from 0 to 50 % in 25 mL. The column was then washed with 1 M NaCl in 10 mL and re-equilibrated by applying 10 mL of the starting buffer. A flow rate of 1 mL/min was maintained during the entire process. Fractions with β-xylosidase activity were collected, pooled together, dialyzed and concentrated by ultrafiltration using 50 kDa cutoff Amicon Ultra-15 centrifugal devices (Merck-Millipore). The purified enzyme was stored at 4 °C.
The production of the native enzyme from T. amestolkiae and its further purification were carried out as described previously .
Characterization of rBxTW1
The enzyme was treated with endoglycosidase H (Endo H; Roche) in order to remove any N-glycosylation, according to manufacturer’s instructions. Purified enzymes, before and after N-deglycosylation, were analyzed by SDS-PAGE, mass spectrometry and size exclusion chromatography. Proteins were loaded onto 10 % polyacrylamide gels and stained with Coomassie brilliant blue R-250 (Sigma-Aldrich). Precision Plus protein dual color standards (Bio-Rad) were used in order to estimate its molecular mass. The accurate molecular mass and homogeneity of the pure enzyme were determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) (Autoflex III, Bruker Daltonics) externally calibrated with Bovine Albumin from Sigma, covering the range from 30 to 150 kDa. The molecular mass in native conditions was studied by size-exclusion chromatography on a Superose 12 HR 10/30 column (GE Healthcare). Both MALDI-TOF and size-exclusion chromatography were performed as described previously .
The effect of temperature and pH were studied in terms of stability and maximal activity. These assays were carried out by using 10 μg/mL of purified rBxTW1 and 0.1 % BSA, in order to get reproducible results regardless of the enzyme concentration .
The effect of pH was analyzed in a range from 2.2 to 7 for enzyme optimum activity and from 2.2 to 9 for enzyme stability. The selected buffers for each segment of the range were glycine–HCl (pH 2.2–3), sodium formate (pH 3–4), sodium acetate (pH 4–5.5), sodium histidine (5.5–7) sodium phosphate (pH 8) and Britton Robinson (pH 9) . pH stability was determined by incubating the samples at 4 °C for 72 h, measuring β-xylosidase activity periodically in standard conditions.
The optimal temperature was analyzed by assaying β-xylosidase activity in 5 and 10 min reactions from 30 to 80 °C. Protein thermostability was described from its T50 value, a parameter defined as the temperature at which the enzyme loses 50 % activity after 10 min of incubation. The enzyme was incubated at 16 different temperatures along a range from 30 to 70 °C. Then, it was cooled at 4 °C for 10 min and rewarmed to room temperature for 5 min. The residual β-xylosidase activity was determined, taking the maximum measured value as 100 %.
Kinetics of rBxTW1 was evaluated against several synthetic and natural substrates for xylanolytic enzymes. The enzyme was assayed by using pNPX, p-NP-α-l-arabinopyranoside and p-NP-α-l-arabinofuranoside (Sigma-Aldrich). The analysis was also carried out against xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose (Megazyme) together with beechwood xylan (Sigma-Aldrich).
Kinetics for each specific substrate were obtained by using increasing substrate concentrations from 0.078 to 20 mM. The activity data were fitted by least-squares to the Lineweaver–Burk linear equation of the Michaelis–Menten model. The effect of product inhibition from xylose was also determined by evaluating the pNPX hydrolysis in the presence of 2.5, 5 and 10 mM xylose and obtaining the corresponding K i .
To determine monosaccharide composition, protein samples were first hydrolyzed with 3 M trifluoroacetic acid (TFA, 121 °C, 1 h), and derivatized and analyzed as reported by Bernabé et al. . The linkage types in the glycan chains of the protein were determined after methylation analysis of dry samples (1–3 mg), dissolved in dimethyl sulfoxide and processed according to the method of Ciucanu and Kerek . The per-O-methylated polysaccharides were hydrolyzed with 3 M TFA, derivatized to their corresponding partially methylated alditol acetates, and analyzed by gas chromatography–mass spectrometry as described elsewhere .
Specificity test of potential transxylosylation acceptors
A screening was carried out in order to evaluate the potential of several compounds to act as acceptors in transxylosylation reactions catalyzed by rBxTW1. The assay was performed in accordance with Blanchard and Withers  with minor variations. The enzyme was first inactivated as its 2-fluoroglucosyl species by incubating 150 µg/mL rBxTW1 in the presence of 5 mM 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-d-xylopyranoside, 5 % dimethyl sulfoxide (DMSO), and 25 mM sodium phosphate (pH 6) in a final volume of 200 μL, at room temperature for 1 h. The sample was dialyzed by ultrafiltration with 10 kDa cutoff Viva spin 500 µL centrifugal filter units (Vivaproducts) in order to remove the excess of inhibitor. The final concentration of inactivated enzyme was corroborated by absorption at 280 nm. An aliquot of the purified, inactivated enzyme was added to the wells of a 96-well plate together with each compound to be screened and the buffer. The final reaction mix was composed of 2.9 μg/mL inactivated rBxTW1, 25 mM sodium phosphate buffer (pH 6), 0.1 % BSA and 20 mM or 40 % saturation of the potential acceptor. pH 6 was selected instead of the standard pH 5, in order to directly follow the release of pNP with time. Controls of non-inactivated enzyme and inactivated enzyme without any potential acceptor were included in triplicate.
The plate was incubated at room temperature for 1 h. Then, pNPX was added at a final concentration of 1 mM and the continuous change in absorbance of each well was measured at 400 nm and 40 °C for 1 h in a plate reader (Molecular Devices Spectra Max 190 Reader). Compounds leading to higher rate of recovery from inhibition than the non-acceptor control were considered positive hits and potential acceptors for transxylosylation by rBxTW1.
A library of 87 compounds was screened in order to find potential transxylosylation acceptors for rBxTW1. The assayed compounds were as follows: methanol; ethanol; 1-propanol; 2-propanol; 1-butanol; 1-pentanol; 1-hexanol; cyclohexanol; 1-octanol; 2-mercaptoethanol, 2-methoxyethanol; 3-mercapto-1-propanol; 5-hexyne-1-ol; 1-ethynylcyclohexanol; 1-adamantanemethanol; 1-pyrenemethanol; 1,2-ethanediol; 1,3-propanediol; phenol; phenethyl alcohol; o-phenylphenol; p-phenylphenol; 4-(hexyloxy)phenol; p-methoxyphenol; p-vinylphenol; resorcinol; phloroglucinol; gallic acid; caffeic acid; 1-naphtol; 2-naphtol; DL-threitol; l-erythritol; l-arabitol; d-arabitol; d-sorbitol; d-galactitol; d-mannitol; myo-inositol; phenyl β-d-glucopyranoside; phenyl β-d-galactopyranoside; p-nitrophenyl α-d-xylopyranoside; p-nitrophenyl α-l-arabinopyranoside; p-nitropheynl α-d-galactopyranoside; p-nitrophenyl α-d-mannopyranoside;; p-nitrophenyl β-d-glucopyranoside; p-nitropheynl β-d-galactopyranoside; p-nitrophenyl β-d-mannopyranoside; p-nitrophenyl β-d-fucopyranoside; p-nitrophenyl β-d-glucuronide; p-nitrophenyl β-d-cellobioside; p-nitrophenyl β-d-lactopyranoside; 4-methylumbilliferyl β-d-xylopyranoside; 4-methylumbelliferyl β-d-glucopyranoside; 4-methylumbelliferyl β-d-galactopyranoside; 4-methylumbelliferyl β-d-cellobiopyranoside; d-xylose; l-arabinose; d-lyxose; d-ribose; d-glucose; d-glucal; d-galactose; d-galactal; d-mannose; 1,5-anhydro-d-glucitol; d-tagatose; d-allose; l-sorbose; l-rhamnose; l-fucose; d-fructose; sucrose; d-maltose; α-lactose; α,α‐trehalose; d-cellobiose; gentiobiose; maltotriose; d-raffinose; l-serine; l-threonine; l-tyrosine; l-asparagine; l-arginine; l-cysteine. Stock solutions of these compounds were made in water to a final concentration, where possible, of 100 mM; some of these solutions were saturated.
2,6-Dihydroxynaphthalene as transxylosylation acceptor
The role of 2,6-DHN (Sigma-Aldrich) as a transxylosylation acceptor for rBxTW1 was assayed. The compound was added to the reaction at a final concentration of 3 g/L together with 0.01 g/L rBxTW1, 20 mM xylobiose, 0.1 % BSA, 50 mM sodium acetate buffer (pH 5) and 5 % acetonitrile. The reaction was incubated at 50 °C and 1200 rpm for 1 h. Samples were withdrawn at 10 min, 30 min and 1 h. The synthesis of new products was followed by thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC).
TLCs were carried out by using silica gel G/UV254 polyester sheets, (0.2 mm thickness and 40 × 80 mm plate size) provided by Macherey–Nagel in ethyl acetate/methanol/water 10:2:1 (v/v). Detection was performed under 254 nm UV light by comparison of the pattern of spots between the reaction and a control without enzyme.
HPLC analyses were performed on an Agilent 1200 series LC instrument equipped with a ZORBAX Eclipse XDB-C18 column (Agilent). The system was equilibrated in acetonitrile/H2O 10:90 v/v (both containing 0.1 % acetic acid) with a flow of 2 mL/min. To purify the reaction products, acetonitrile concentration increased along a linear gradient from 10 to 20 % for 8 min. Then, the mobile phase mix changed to 95 % acetonitrile for 3 min and finally to 10 % for 3 min in order to respectively wash and re-equilibrate the column. The product peaks were detected by monitoring absorbance at 220 nm from the naphthalene ring and quantification was based on the areas under the peaks. As the reaction products are not commercially available, they were quantified from a calibration curve of 2,6-DHN.
Response surface methodology
Optimization of the reaction conditions for the production of 2-(6-hydroxynaphthyl) β-d-xylopyranoside was attempted by using the response surface methodology. Design-Expert® software version 10.0.1.0 (Stat-Ease Inc. MN, USA) was selected for generating a Box-Behnken design matrix and for the analysis of generated data. Concentration of xylobiose (donor), 2,6-DHN (acceptor) and enzyme, reaction time, temperature and pH were selected as the most significant parameters for xyloside production and included as independent variables for the development of the experimental design.
In this approach the parameters are studied at three levels: low, middle and high, leading to optimal values with a smaller number of designed experiments. The software then generates a polynomial quadratic equation from the obtained data which analyzes the effect of the independent variables on the response [28, 29].
The variables and levels assayed are displayed in Table 4. Maximum and minimum levels were previously determined by using one factor at a time approach (data not shown).
The reaction was scaled-up to 10 mL in conditions of high production predicted by the multiparametric model: 3 g/L 2,6-DHN, 50 mM xylobiose, 0.15 g/L rBxTW1 50 mM sodium acetate buffer (pH 5.5) and 39.5 °C for 80 min. The reaction was carried out at 1200 rpm and stopped by heating at 100 °C for 5 min.
The reaction mix was concentrated by speed vacuum before being loaded onto a semi-preparative column (Mediterranea sea18 TR-010006, Teknokroma) in order to be purified by HPLC. The system was equilibrated in acetonitrile/H2O 10:85 v/v (both containing 0.1 % acetic acid) and a flow rate of 2 mL/min was maintained during the entire process. The products were eluted by applying a gradient from 15 to 20 % acetonitrile for 13 min. Then the concentration of acetonitrile was raised up to 95 % for 3 min in order to wash the column. Finally, the system was allowed to re-equilibrate in the starting conditions for 4 min.
Product formation was followed by monitoring absorbance at 220 nm. After collection and solvent evaporation, products were stored at −20 °C.
Nuclear magnetic resonance (NMR)
The identification of the xylosides formed from 2,6-dihydroxynaphthalene was carried out by NMR. Data were acquired at 308K, using a Bruker AVANCE 500 MHz spectrometer. 1D 1H NMR spectra, 2D homonuclear TOCSY (60 ms mixing time), and 2D heteronuclear 1H-13C HSQC experiments were acquired, in order to assign all NMR signals. For 1D 1H, TOCSY, and 1H-13C HSQC, the standard zg, zgesp, dipsi2phpr, and hsqcedetgp pulse sequences implemented in TOPSPIN 2.1 acquisition software (Bruker) were employed.
Results and discussion
Expression of rBxTW1 in P. pastoris
The gene bxtw1 was successfully expressed in P. pastoris. The mature bxtw1 sequence without signal peptide and introns comprised 2337 bp, including the native stop codon. After the screening, clone 18 from strain GS115 proved to be the best rBxTW1 producer and was selected for enzyme production in liquid cultures.
β-Xylosidase activity reached a maximum of 8 U/mL in 10-day-old YEPS cultures (Fig. 2), which is an excellent value when compared with those reported for other fungal β-xylosidases of the GH3 family produced in P. pastoris (Table 1).
The recombinant rBxTW1 was completely purified by FPLC after a single step of cation-exchange chromatography with a yield of 91.5 % and a degree of purification of 1.9. The above data represent a huge increase in both maximal activity and protein purification yield respect to the native enzyme, which is produced at 0.8 U/mL and purified after three chromatographic steps, with a final yield of 10.8 % . These results indicate that P. pastoris is an appropriate host for producing rBxTW1.
Characterization of rBxTW1
The isoelectric point of the recombinant enzyme was determined to be 8.4, more basic than that of the native enzyme, which is probably due to their different glycosylation pattern . The molecular mass estimated by SDS-PAGE was around 100 kDa. The difference between this value and that predicted from the amino acid sequence of the protein (84.650 kDa) suggested that rBxTW1 was glycosylated, which is common for proteins expressed in P. pastoris . The enzyme was N-deglycosylated with Endo H and subsequently analyzed by SDS-PAGE and MALDI-TOF MS (Fig. 3). As expected, the molecular mass decreased, displaying a value of 91 kDa by MALDI-TOF. The difference with respect to the theoretical 84.650 kDa may be attributed to O-glycosylation which are also introduced by the yeast but not removed with Endo H. These assays confirmed that P. pastoris was producing the enzyme as a glycoprotein with approximately 10 % N-glycosylation. On the other hand, the measured molecular mass of rBxTW1 determined by size exclusion chromatography was around 160 kDa, suggesting that in aqueous medium this protein forms a non-covalent dimer, as reported for the native enzyme . As the values obtained by size exclusion chromatography are influenced by the shape of the proteins, and are not fully accurate, hereinafter the correct average mass of the glycosylated monomeric rBxTW1 will be assumed to be 100 kDa, as determined by SDS-PAGE, and that of the dimeric form 200 kDa. This molecular mass value is the one used to calculate the kinetic parameters of the β-xylosidase.
The kinetic properties of rBxTW1 were determined against pNP-pentoses, XOS and beechwood xylan (Table 2). The enzyme showed high affinity for pNPX and XOS, especially when compared with the increased K m displayed in the hydrolysis of pNP-α-l-arabinopyranoside and pNP-α-l-arabinofuranoside. Competitive inhibition by product (xylose) was analyzed resulting in a K i of 1.7 ± 0.3 mM when pNPX was used as substrate.
The biochemical and kinetic properties of rBxTW1, namely the general profiles for optimal pH (Fig. 4a) and temperature (Fig. 4b), substrate specificity, and high affinity for pNPX and XOS, were very similar to those reported for the native enzyme . However, the temperature for maximal activity decreased from 70 to 60 °C, T50 was around 9° lower and the enzyme lost its stability at basic pH, indicating the lower stability of the recombinant form. In addition, a decrease of its maximum velocity was also observed. Similar results have been reported by Wei et al. expressing a fungal GH3 β-glucosidase in P. pastoris , which were attributed to the fact that N-glycans usually introduced by this yeast are larger than those of fungi.
The native and recombinant BxTW1 enzymes both carry glycosylation at approx. 10 % of their mass . However, the compositions of their glycans are different (Table 3). Both enzymes had mannose as the major monosaccharide, but the carbohydrate chains of the β-xylosidase secreted by T. amestolkiae also contained substantial amounts of glucose and N-acetyl-glucosamine, while in rBxTW1 mannose residues represented more than 90 % of its total sugar content, which is consistent with the hyper-mannosylation described for yeasts . In addition, these enzymes also differed in the linkage types of their glycan component (Table 3). The interpretation of the data will be focused on the mannose content, which represents the main part of the glycan and has been deeply studied in the N-glycosylation patterns reported for fungi and yeasts [34, 35]. Mannose residues with a single link assigned (Manp-(1→) represent the non-reducing ends of oligosaccharide chains, two links meant a residue for chain extension (→2)-Manp-(1→; →3)-Manp-(1→) while three or four links implied a branching point. Based on this, there are important differences between the recombinant and the native fungal enzyme. rBxTW1 displays about 35 % of →2)-Manp-(1→ units and low content of the other types of extension-residues, while in the native enzyme the proportion of →2)-Manp-(1→ and →6)-Manp-(1→ units is similar (18 and 16 % respectively). On the other hand, the native fungal enzyme was found to have 6 % branching points for triple branching, whereas these residues represent less than 1 % in rBxTW1. These data suggest that the glycan in the enzyme from T. amestolkiae has a highly branched structure, but with shorter chains in comparison with the recombinant enzyme, which also displayed more homogeneity.
The differences in carbohydrate content and structure between the two enzymes are in good accordance with the hypothesis proposed by Wei et al. , suggesting that the long mannose-chains incorporated by P. pastoris, in opposition to the shorter and more heterogeneous fungal glycosylation, may be the primary cause of the differences between the two glycosidases.
However, the drawbacks observed for the recombinant enzyme are compensated by its high production levels and purification yields, since expression in P. pastoris led to an increase of 85-fold on the recovered activity units per volume of culture.
rBxTW1 as a versatile tool for transxylosylation of bioactive compounds
In order to fully determine the biotechnological potential of the recombinant enzyme, its transxylosylation capacities were analyzed. To test if rBxTW1 kept the transglycosylation potential exhibited by the native enzyme , and in particular to assess its aglycone preference, a rapid screen of aglycone specificity was performed, according to the methodology developed by Blanchard and Withers . The library of tested compounds included confirmed acceptors for the native enzyme, representative carbohydrates, alcohols and aromatic compounds together with some amino acids and other chemicals readily available in the laboratory.
The results from this study are gathered as a heat map at Fig. 5. The high number of positive hits suggested that the recombinant enzyme retained the broad acceptor versatility reported for BxTW1. Sugar alcohols seemed to be very good acceptors for both enzymes. The presence of gallic acid and p-nitrophenyl β-d-glucuronide among the few negative hits suggested that carboxylic acids are not suitable acceptors for being transxylosylated by rBxTW1. Xylobiose and xylose also appeared as apparent negative hits, which is at first sight surprising because both carbohydrates are well known acceptors for BxTW1 (; Additional file 1). This is due to the fact that xylose and xylobiose are, respectively, a competitive inhibitor and a substrate for rBxTW1, thus competed with pNPX in the assay, thereby appearing not to be acceptors. The same could be true for p-nitrophenyl β-d-glucuronide. This emphasizes the need to carry out a parallel screen with non-inactivated enzyme to check for potential inhibitors of this class, so that they can be investigated separately.
Among the clearest positive hits there were at least three remarkable compounds for their potential biotechnological importance in terms of transglycosylation. 4-vinylphenol is a volatile phenolic compound found in wine, beer and other non-alcoholic beverages as orange juice. It is released from precursors during storage, being one of the main causes of off-flavor problems. Transxylosylation may provide a solution to this industrial concern by converting this molecule into a stable non-volatile glycoside [36, 37]. The second interesting compound is 1-pentanol that can be transformed into pentyl xyloside, an alkyl xyloside that, like others, may be a useful surfactant . Regarding phenethyl alcohol, its antimicrobial properties are well known and natural glycosides of this compound have been found and reported to display immunosuppressive responses [39, 40]. The chemical synthesis of glycosides from 4-vinylphenol, 1-pentanol and phenethyl alcohol needs a minimum of three steps Therefore, the easily accessible synthesis of those xylosides and related derivatives by transxylosylation and the study of their potential biological activity may lead to a better understanding of these properties. To the best of our knowledge, there is only one precedent of using phenethyl alcohol  and no previous report concerning the use of 4-vinylphenol as acceptors for enzymatic transglycosylation.
Enzymatic synthesis of 2-(6-hydroxynaphthyl) β-d-xylopyranoside and production enhancement by response surface methodology
The screening of the aglycone specificity also revealed 2-naphthol and especially 1-naphthol as potential transxylosylation acceptors for rBxTW1. This was an unexpected result since the bulky naphthalene molecule might not have been expected to fit in enzyme active site. These data prompted us to test for the formation of a new xyloside by using 2,6-DHN as an acceptor, due to the existing structural similarities between this compound and 1- and 2-naphtol, and because the potential transxylosylation product would be 2-(6-hydroxynaphthyl) β-d-xylopyranoside, a well known antiproliferative compound. The three compounds contain a naphthalene ring which is monohydroxylated in the case of 1- and 2-naphtol and dihidroxylated for 2,6-DHN. The absence of any other significant difference led us to expect that this compound would also work as transxylosylation acceptor for rBxTW1. The desired xyloside, 2-(6-hydroxynaphthyl) β-d-xylopyranoside, functions as selective inhibitor of transformed or tumor-derived cell growth by acting as an alternative primer for heparan sulfate synthesis. These interesting properties had been widely demonstrated in vitro by adding the compound to cultures of normal and transformed cell lines such as HFL-1 cells (human fetal lung fibroblasts), 3T3 A31 cells (mouse 3T3 fibroblasts) and T24 cells (human bladder carcinoma cells) and in vivo with a subcutaneous tumor model in mice [19, 42].
Since the selected library did not include 2,6-DHN, the role of this compound as acceptor for rBxTW1 was evaluated by the direct detection of the new product in TLC, which is a better approach when assaying single compounds. With the set of conditions initially fixed for the transxylosylation assay, a faint new spot, probably corresponding to the reaction product, was observed upon developing the TLC plate (data not shown). HPLC analysis of the reaction mixture allowed estimation of the concentration of the hypothetical new xyloside to be 0.19 mM. The reaction conditions were further optimized using a response surface method, specifically BBD, in order to enhance the production of 2-(6-hydroxynaphthyl) β-d-xylopyranoside. The matrix of the experiments generated by the BBD approach and the outcomes from this analysis are collected in Table 4. The BBD matrix with the production data was analyzed by Design-Expert® software and fitted to the following quadratic model equation:
[Product] = − 3.82 + 0.395A + 0.128B + 1.41 × 10−3C + 1.51 × 10−2D + 1.12E + 0.144F − 5.69 × 10−3AB + 2.06 × 10−4AC + 2.38 × 10−3AD + 3.32AE + 7.58 × 10−3AF − 5.46 × 10−6BC − 2.36 × 10−4BD −2.88 × 10−2BE + 1.77 × 10−3BF + 7.48 × 10−5CD + 5.99 × 10−2CE + 1.13 × 10−4CF + 1.90 × 10−2DE + 3.72 × 10−4DF + 0.629EF − 6.54 × 10−2A2 − 1.23 × 10−3B2 − 5.16 × 10−5C2 − 1.31 × 10−4D2 − 60.8E2 −3.34 × 10−2F2
A: [2,6-DHN] (g/L); B: Temperature (°C); C: [Xylobiose] (mM); D: Time (min); E: [Enzyme] (g/L); F: pH.
The model predicted the production of the new xyloside as a function of the concentrations of 2,6-DHN and xylobiose, the amount of added enzyme, the temperature and time of reaction and the pH value. The analysis of variance test calculated by the software (Table 5) validated that the model matched the experimental data.
The Design-Expert® software was used to find the experimental conditions for maximal production predicted by the model within the selected limits for each parameter. The software generated a maximum value of 1.6 mM, which required a reaction mix composed of 3 g/L 2,6-DHN; 100 mM xylobiose; 0.1 g/L rBxTW1 and 50 mM sodium acetate pH 4.8, a temperature of 40.8 °C and a reaction time of 60 min. Under these conditions, the measured product concentration fit the theoretical value of 1.6 mM. Therefore, application of the response surface model enabled an eightfold increase (from 0.19 to 1.6 mM) in xyloside production.
The reaction parameters from the former Box-Behnken adjustment were further changed according to the availability of reactants and enzyme in order to produce the hypothetical product 2-(6-hydroxynaphthyl) β-d-xylopyranoside for its purification and identification. Since pure commercial xylobiose is an expensive substrate, but the enzyme is easily produced and purified, a new estimation was run by the model, decreasing xylobiose concentration from 100 to 50 mM, while no limits were set on enzyme concentration, temperature and reaction time. With these new settings, the predicted optimal conditions included higher enzyme concentration and longer reaction time (0.1 to 0.15 g/L and 60 to 80 min, respectively), while temperature was slightly lower to delay enzyme inactivation and pH changed from 4.8 to 5.5. Under these conditions, a maximum concentration of 1.5 mM was predicted, and an actual value of 1.4 mM was obtained in this assay. This value is close to the 1.6 mM achieved when no limits were applied to xylobiose concentration. In both cases, the empirical values corroborated the theoretical predicted data.
The reaction mix was concentrated and analyzed by semi-preparative HPLC, purifying a major product peak and a minor one (Product 2) that was not previously detected in analytical-scale reactions. The yield of this by-product was, however, very low (0.2 mM). Product 2 eluted during the acetonitrile gradient, before the hypothetical 2-(6-hydroxynaphthyl) β-d-xylopyranoside (Product 1), suggesting that Product 2 may have incorporated a second xylose unit and thereby acquired increased polarity. If this assumption is true, the most probable scenarios are a second xylose unit attached either to the remaining free hydroxyl group of the 2-(6-hydroxynaphthyl) β-d-xylopyranoside or to the xylose already present. As the demonstrated regioselectivity of the native enzyme  is expected to persist in the recombinant form, the latter option may occur through a β(1 → 4) linkage, converting the xylose substituent into xylobiose. For complete identification of products, they were analyzed by NMR as follows.
Structural elucidation of the 2,6-dihydroxynaphthyl transxylosylation products by NMR
Analysis of 1H and 13C-NMR experiments of the two purified products allowed the elucidation of their structure. As expected, Product 1 was found to be 2-(6-hydroxynaphthyl) β-d-xylopyranoside (Fig. 6a; Table 6) in accordance with data described for the chemically synthesized compound  while product 2, where two different sets of xylose signals appeared, was identified as 2-(6-hydroxynaphthyl) β-d-xylobioside (Fig. 6b; Table 6). The synthesis of the latter product instead of 2,6-dihydroxynaphthalene bis(β-d-xylopyranoside), that due to symmetry of the molecule would have only one set of xylose signals , indicated the preference of the enzyme for the sugar hydroxyl over the second naphthyl alcohol.
As far as we know the synthesis of 2-(6-hydroxynaphthyl) β-d-xylobioside has not been reported previously. The chemical synthesis of this compound would be more challenging than that of the xyloside because of the need to attach the second xylose moiety specifically to the C-4 hydroxyl group of the acceptor sugar . The potential anti-proliferative role of 2-(6-hydroxynaphthyl)-β-d-xylobioside has not yet been investigated and merits attention. Further research using new and cheaper xylose donor will be necessary in order to carry out the required increase of production for the anti-proliferative analyses.
A β-xylosidase from T. amestolkiae was produced in P. pastoris and purified. The final yields were much higher than those attained for the native protein, although some loss of stability and maximum velocity was observed. rBxTW1 displayed remarkable acceptor versatility in transxylosylation, catalyzing the synthesis of a selective antiproliferative compound, 2-(6-hydroxynaphthyl) β-d-xylopyranoside, enzymatically obtained for the first time. Response surface approaches enhanced this production by eightfold. These results evidence the interest of rBxTW1 for transxylosylation of industrially relevant products. Using xylans from lignocellulosic wastes, as cost-effective xylose donors, should be tested for developing a green alternative to current chemical synthesis.
- A600 :
absorbance at 600 nm
fast protein liquid chromatography
matrix-assisted laser desorption ionization-time of flight mass spectrometry
nuclear magnetic resonance
thin layer chromatography
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MND drafted the manuscript and performed the experiments excluding gas chromatography, NMR and mass spectrometry. AP designed and performed the gas chromatography assays and analyzed the data. BFT and FJC designed and performed the NMR assays and analyzed the data. JB designed the DNA cloning, the transformation of Pichia pastoris and the screening for high production clones. ZA and SGW designed the rapid screen of aglycone specificity. LIE and MJM coordinated the study, helped to draft, and critically review the manuscript. All authors read and approved the final manuscript.
We thank Dr. J. Jiménez-Barbero for his help in the development of NMR experiments and Dr. Hongming Chen for the synthesis of 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-d-xylopyranoside. We are grateful for the use of Proteomics and Genomics (ProteoRed-ISCIII network) and Gas Chromatography CIB-CSIC services.
The authors declare that they have no competing interests.
Availability of data and materials
All data on which the conclusions of this study rely are presented in the manuscript.
This work was carried out with funding from projects BIO2015-68387-R, RTC-2014-1777-3 and CTQ2015-64597-C2 from MINECO and S2013/MAE-2972 from Comunidad de Madrid, as well as from the Natural Sciences and Engineering Research Council of Canada. M. Nieto-Domínguez thanks the MINECO for an FPU fellowship.
Additional file 1. Xylose and xylobiose as transxylosylation acceptors. Transxylosylation reactions catalyzed by rBxTW1 using xylose or xylobiose as acceptors. The analysis by TLC of the samples is displayed in order to demonstrate the synthesis of the transxylosylation products.
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Nieto-Domínguez, M., Prieto, A., Fernández de Toro, B. et al. Enzymatic fine-tuning for 2-(6-hydroxynaphthyl) β-d-xylopyranoside synthesis catalyzed by the recombinant β-xylosidase BxTW1 from Talaromyces amestolkiae . Microb Cell Fact 15, 171 (2016). https://doi.org/10.1186/s12934-016-0568-6
- Pichia pastoris
- 2-(6-hydroxynaphthyl) β-d-xylopyranoside
- Response surface methodology
- Box-Behnken design