Table 2 Kinetic parameters of rBxTW1
Substrate | K m (mM) | V max (U/mg) | k cat (s−1) | k cat /K m (mM−1 s−1) |
|---|---|---|---|---|
pNPX | 0.20 ± 0.01 | 20.8 ± 0.3 | 69.3 | 336 |
pNP-α-l-arabinopyranoside | 1.6 ± 0.1 | 8.3 ± 0.2 | 28 | 17 |
pNP-α-l-arabinofuranoside | 4.3 ± 0.3 | 14.9 ± 0.4 | 49.8 | 12 |
Xylobiose | 0.51 ± 0.03 | 26.4 ± 0.3 | 87.9 | 170 |
Xylotriose | 0.19 ± 0.01 | 9.0 ± 0.1 | 30 | 160 |
Xylotetraose | 0.20 ± 0.01 | 6.34 ± 0.06 | 21.1 | 100 |
Xylopentaose | 0.22 ± 0.01 | 4.48 ± 0.05 | 14.9 | 68 |
Xylohexaose | 0.21 ± 0.02 | 4.27 ± 0.07 | 14.2 | 69 |
Polymeric substrate | K m (mg/mL) | V max (U/mg) | k cat (s−1) | k cat /K m (mM−1 s−1) |
|---|---|---|---|---|
Xylan | 8.9 ± 0.3 | 21.4 ± 0.3 | 71.5 | – |