Table 3 Monosaccharide distribution and linkage types present in the carbohydrate moiety of the native and recombinant BxTW1
Characteristic ions (m/z) | Content (%) | ||
|---|---|---|---|
Native | Recombinant | ||
Monosaccharide | |||
Mannose | 43, 115, 145, 187, 217, 259, 361 | 68.7 | 91.9 |
Glucose | 43, 115, 145, 187, 217, 259, 361 | 13.1 | 1.9 |
Glucosamine | 43, 84, 102, 144, 156, 258, 318, 360 | 12.1 | 6.2 |
Galactose | 43, 115, 145, 187, 217, 259, 361 | 6.1 | – |
Deduced linkage | |||
Manp-(1→ | 87, 88, 102, 118, 129, 161, 205 | 27.5 | 29.0 |
Galp-(1→ | 89, 101, 102, 118, 162, 205 | 3.0 | 0.4 |
→2)-Manp-(1→ | 87, 88, 101, 129, 130, 161, 190 | 18.1 | 35.3 |
→3)-Manp-(1→ | 101, 118, 129, 161, 234 | 0.0 | 2.0 |
→6)-Manp-(1→ | 87, 88, 99, 102, 118, 129, 162, 189 | 16.4 | 3.4 |
→2,3)-Manp-(1→ | 101, 129, 161, 202, 262 | 0.0 | 1.3 |
→2,6)-Manp-(1→ | 117, 118, 129, 130, 189, 190 | 1.9 | 14.0 |
→3,6)-Manp-(1→ | 118, 129, 189, 174, 234 | 14.5 | 8.1 |
→3,4,6)-Manp-(1→ | 118, 139 | 6.3 | 0.5 |
→4)-GlcpNH2-(1→ | 117, 159, 233 | 12.3 | 4.6 |