Table 4 Box–Behnken experimental design for optimization of 2-(6-hydroxynaphthyl)-β-d-xylopyranoside
2,6-DHN (g/L) | Temperature (°C) | Xylobiose (mM) | Time (min) | Enzyme (g/L) | pH | [Product] (mM) |
|---|---|---|---|---|---|---|
0.30 | 40 | 60 | 10 | 0.055 | 4.1 | 0.04 |
3.00 | 40 | 60 | 10 | 0.055 | 4.1 | 0.45 |
0.30 | 60 | 60 | 10 | 0.055 | 4.1 | 0.05 |
3.00 | 60 | 60 | 10 | 0.055 | 4.1 | 0.37 |
0.30 | 40 | 60 | 60 | 0.055 | 4.1 | 0.13 |
3.00 | 40 | 60 | 60 | 0.055 | 4.1 | 1.09 |
0.30 | 60 | 60 | 60 | 0.055 | 4.1 | 0.12 |
3.00 | 60 | 60 | 60 | 0.055 | 4.1 | 0.55 |
1.65 | 40 | 20 | 35 | 0.010 | 4.1 | 0.13 |
1.65 | 60 | 20 | 35 | 0.010 | 4.1 | 0.05 |
1.65 | 40 | 100 | 35 | 0.010 | 4.1 | 0.13 |
1.65 | 60 | 100 | 35 | 0.010 | 4.1 | 0.07 |
1.65 | 40 | 20 | 35 | 0.100 | 4.1 | 0.34 |
1.65 | 60 | 20 | 35 | 0.100 | 4.1 | 0.41 |
1.65 | 40 | 100 | 35 | 0.100 | 4.1 | 0.80 |
1.65 | 60 | 100 | 35 | 0.100 | 4.1 | 0.83 |
1.65 | 50 | 20 | 10 | 0.055 | 2.2 | 0.23 |
1.65 | 50 | 100 | 10 | 0.055 | 2.2 | 0.11 |
1.65 | 50 | 20 | 60 | 0.055 | 2.2 | 0.37 |
1.65 | 50 | 100 | 60 | 0.055 | 2.2 | 0.55 |
1.65 | 50 | 20 | 10 | 0.055 | 6.0 | 0.26 |
1.65 | 50 | 100 | 10 | 0.055 | 6.0 | 0.29 |
1.65 | 50 | 20 | 60 | 0.055 | 6.0 | 0.48 |
1.65 | 50 | 100 | 60 | 0.055 | 6.0 | 0.80 |
0.30 | 50 | 60 | 10 | 0.010 | 4.1 | 0.00 |
3.00 | 50 | 60 | 10 | 0.010 | 4.1 | 0.07 |
0.30 | 50 | 60 | 60 | 0.010 | 4.1 | 0.05 |
3.00 | 50 | 60 | 60 | 0.010 | 4.1 | 0.31 |
0.30 | 50 | 60 | 10 | 0.100 | 4.1 | 0.09 |
3.00 | 50 | 60 | 10 | 0.100 | 4.1 | 0.85 |
0.30 | 50 | 60 | 60 | 0.100 | 4.1 | 0.11 |
3.00 | 50 | 60 | 60 | 0.100 | 4.1 | 1.31 |
1.65 | 40 | 60 | 35 | 0.010 | 2.2 | 0.18 |
1.65 | 60 | 60 | 35 | 0.010 | 2.2 | 0.00 |
1.65 | 40 | 60 | 35 | 0.100 | 2.2 | 0.67 |
1.65 | 60 | 60 | 35 | 0.100 | 2.2 | 0.19 |
1.65 | 40 | 60 | 35 | 0.010 | 6.0 | 0.13 |
1.65 | 60 | 60 | 35 | 0.010 | 6.0 | 0.02 |
1.65 | 40 | 60 | 35 | 0.100 | 6.0 | 0.77 |
1.65 | 60 | 60 | 35 | 0.100 | 6.0 | 0.49 |
0.30 | 50 | 20 | 35 | 0.055 | 2.2 | 0.05 |
3.00 | 50 | 20 | 35 | 0.055 | 2.2 | 0.49 |
0.30 | 50 | 100 | 35 | 0.055 | 2.2 | 0.10 |
3.00 | 50 | 100 | 35 | 0.055 | 2.2 | 0.66 |
0.30 | 50 | 20 | 35 | 0.055 | 6.0 | 0.06 |
3.00 | 50 | 20 | 35 | 0.055 | 6.0 | 0.66 |
0.30 | 50 | 100 | 35 | 0.055 | 6.0 | 0.11 |
3.00 | 50 | 100 | 35 | 0.055 | 6.0 | 0.67 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.68 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.69 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.67 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.67 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.70 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.65 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.67 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.66 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.66 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.68 |
1.65 | 50 | 60 | 35 | 0.055 | 4.1 | 0.66 |