Improving ethanol yield in acetate-reducing Saccharomyces cerevisiae by cofactor engineering of 6-phosphogluconate dehydrogenase and deletion of ALD6
© Papapetridis et al. 2016
Received: 29 January 2016
Accepted: 13 April 2016
Published: 26 April 2016
Acetic acid, an inhibitor of sugar fermentation by yeast, is invariably present in lignocellulosic hydrolysates which are used or considered as feedstocks for yeast-based bioethanol production. Saccharomyces cerevisiae strains have been constructed, in which anaerobic reduction of acetic acid to ethanol replaces glycerol formation as a mechanism for reoxidizing NADH formed in biosynthesis. An increase in the amount of acetate that can be reduced to ethanol should further decrease acetic acid concentrations and enable higher ethanol yields in industrial processes based on lignocellulosic feedstocks. The stoichiometric requirement of acetate reduction for NADH implies that increased generation of NADH in cytosolic biosynthetic reactions should enhance acetate consumption.
Replacement of the native NADP+-dependent 6-phosphogluconate dehydrogenase in S. cerevisiae by a prokaryotic NAD+-dependent enzyme resulted in increased cytosolic NADH formation, as demonstrated by a ca. 15 % increase in the glycerol yield on glucose in anaerobic cultures. Additional deletion of ALD6, which encodes an NADP+-dependent acetaldehyde dehydrogenase, led to a 39 % increase in the glycerol yield compared to a non-engineered strain. Subsequent replacement of glycerol formation by an acetate reduction pathway resulted in a 44 % increase of acetate consumption per amount of biomass formed, as compared to an engineered, acetate-reducing strain that expressed the native 6-phosphogluconate dehydrogenase and ALD6. Compared to a non-acetate reducing reference strain under the same conditions, this resulted in a ca. 13 % increase in the ethanol yield on glucose.
The combination of NAD+-dependent 6-phosphogluconate dehydrogenase expression and deletion of ALD6 resulted in a marked increase in the amount of acetate that was consumed in these proof-of-principle experiments, and this concept is ready for further testing in industrial strains as well as in hydrolysates. Altering the cofactor specificity of the oxidative branch of the pentose-phosphate pathway in S. cerevisiae can also be used to increase glycerol production in wine fermentation and to improve NADH generation and/or generation of precursors derived from the pentose-phosphate pathway in other industrial applications of this yeast.
The intensive use of fossil resources by mankind presents one of the great challenges of our time and many research efforts focus on seeking sustainable alternatives for petrochemistry-based production of transport fuels and chemicals. One of these alternatives is the microbial conversion of hydrolysates of lignocellulosic plant biomass into fuel ethanol. Saccharomyces cerevisiae is a major candidate for this application, because of its naturally high ethanol yield on sugar and tolerance to inhibitors and low pH values [22, 38, 64]. In addition to these natural attributes, robust performance of S. cerevisiae in lignocellulosic hydrolysates requires tolerance to the organic acids, furans and phenols that are released during biomass pre-treatment.
One of the most important inhibitors released during hemicellulose hydrolysis is acetic acid, whose concentration in lignocellulosic hydrolysates can exceed 10 g L−1 . As for all weak organic acids in solution, the relative concentrations of the un- and dissociated (acetate) forms of acetic acid are determined by its acid-dissociation constant (pKa) and by the extant pH. Industrial fermentation processes with S. cerevisiae are typically performed at pH values close to the pKa of acetic acid (4.75). This implies that a substantial fraction of the acid will be present in its non-dissociated form, which can diffuse across the yeast plasma membrane. Upon entry into the near-neutral yeast cytosol (pH 6.5–7 during exponential growth ), acetic acid will dissociate and release a proton. To avoid acidification of the cytosol, protons have to be expelled by the yeast plasma membrane ATPase. This proton export requires 1 ATP per proton, while additional metabolic energy may be required to expel the acetate anion [44, 46]. At low to moderate concentrations of acetic acid (1–3 g L−1) and at pH values of 4–5, this increased demand for ATP results in lower biomass and glycerol yields and a higher ethanol yield on glucose in anaerobic cultures of S. cerevisiae [2, 27, 46]. However, at higher acetic acid concentrations (or at a lower pH), cells can no longer meet the energy requirements for pH homeostasis and can no longer prevent acidification of the cytosol, leading to inhibition of fermentation and growth [32, 60]. Inhibition by acetic acid is even more pronounced when engineered yeast strains utilise xylose, a major component of lignocellulosic hydrolysates, as a carbon source . The variability in acetic acid sensitivity of glucose- and xylose-grown cultures has been attributed to the sugar fermentation rates with these sugars, with a slower fermentation of xylose constraining the maximum rate of proton export via the plasma-membrane ATPase. In addition to the impact of acetic acid on intracellular pH homeostasis, intracellular accumulation of the acetate anion has been linked to increased oxidative stress and inhibition of key enzymes, such as aldolase , transaldolase and transketolase .
Although removal of acetic acid and other inhibitors from lignocellulosic hydrolysates can be achieved through chemical or biological detoxification, such additional steps are costly and can cause loss of fermentable substrate [28, 43, 47, 59]. Therefore, development of stress-resistant yeast strains has received considerable attention. Acetic acid tolerance, which differs among S. cerevisiae strains, is a multi-gene trait [23, 35, 58] which has been the objective of metabolic and evolutionary engineering studies [51, 55, 70]. Guadalupe-Medina et al.  first explored the in situ reduction of acetic acid to ethanol as an alternative strategy to combat acetic acid toxicity. Under anaerobic conditions, wild-type strains of S. cerevisiae cannot metabolise acetic acid . Expression of the E. coli mhpF gene, which encodes an NAD+-dependent acetylating acetaldehyde dehydrogenase, introduced a pathway for NADH-dependent reduction of acetic acid to ethanol into S. cerevisiae. When combined with inactivation of the GPD1 and GPD2 genes, which encode glycerol-3-phosphate dehydrogenase and are essential for glycerol production, reoxidation of NADH formed in biosynthesis was coupled to the reduction of acetic acid to ethanol . This approach completely abolished the formation of glycerol which, after biomass and CO2, is the most important by-product of industrial ethanol production. The ensuing 13 % increase in the apparent ethanol yield on sugar was caused by the elimination of carbon loss to glycerol and the conversion of acetic acid to additional ethanol. In addition to improving the ethanol yield on sugar, this metabolic engineering strategy enabled a partial in situ detoxification of acetic acid by the yeast. However, the amount of acetic acid that can be converted by the engineered yeast strain is limited by the amount of NADH resulting from biosynthesis which, in anaerobic cultures of wild-type yeast, is reoxidized via the formation of glycerol [63, 68].
The goal of the present study is to explore a metabolic engineering strategy for increasing the amount of acetic acid that can be reduced to ethanol in anaerobic S. cerevisiae cultures. The proposed strategy aims to increase the formation of surplus cytosolic NADH in biosynthesis by replacing the native NADP+-dependent yeast 6-phosphogluconate dehydrogenase (encoded by GND1 and GND2 ) with a prokaryotic NAD+-dependent enzyme. 6-phosphogluconate dehydrogenase (6-PGDH) catalyses the oxidative decarboxylation of 6-phospho-D-gluconate to D-ribulose-5-phosphate. In S. cerevisiae, this reaction is strictly NADP+-dependent and part of the oxidative pentose-phosphate pathway, the major NADPH-providing pathway in this yeast [4, 54]. First, the predicted impact of this strategy on increasing NADH availability was evaluated by a theoretical stoichiometric analysis. Subsequently, three candidate genes encoding heterologous NAD+-dependent 6-phosphogluconate dehydrogenases were tested for functional expression in S. cerevisiae. One of these genes was then expressed in a reference strain of S. cerevisiae and in strain backgrounds that contained additional modifications. The final set of strains also included strains in which the glycerol production pathway had been replaced by an acetate reduction pathway. The physiological impact of these redox-cofactor engineering interventions on product yields and acetate conversion was quantitatively analysed in anaerobic bioreactor cultures.
Strains and maintenance
Saccharomyces cerevisiae strains used in this study
MATa MAL2-8c SUC2
MATa MAL2-8c SUC2 can1::cas9-natNT2
MATa MAL2-8c SUC2 can1::cas9-natNT2 gnd2Δ
MATa MAL2-8c SUC2 can1::cas9-natNT2 ald6Δ
MATa MAL2-8c SUC2 can1::cas9-natNT2 gnd2Δ gnd1::gndA
MATa MAL2-8c SUC2 can1::cas9-natNT2 gnd2Δ gnd1::6pgdh
MATa MAL2-8c SUC2 can1::cas9-natNT2 gnd2Δ gnd1:gox1705
MATa MAL2-8c SUC2 can1::cas9-natNT2 gnd2Δ gnd1::gndA ald6Δ
MATa MAL2-8c SUC2 can1::cas9-natNT2 gnd2Δ gnd1::gndA ald6Δ gpd2::eutE
MATa MAL2-8c SUC2 can1::cas9-natNT2 gnd2Δ gnd1::gndA ald6Δ gpd2::eutE gpd1Δ
MATa MAL2-8c SUC2 can1::cas9-natNT2 gpd2::eutE
MATa MAL2-8c SUC2 can1::cas9-natNT2 gpd2::eutE gpd1Δ
Plasmid and cassette construction
Plasmids used in this study
Delivery vector, p426-TDH3p-eutE
2 μm ori, amdS, SNR52p-gRNA.CAN1.Y-SUP4 t
amdS, gRNA.CAN1-2 μm ori-gRNA.ADE2
2 μm ori, p426-TDH3p-eutE-CYC1t
2 μm ori, amdS, SNR52p-gRNA.GND2.Y-SUP4 t
2 μm ori, amdS, SNR52p-gRNA.GND1.Y-SUP4 t
Delivery vector, TPI1p-gndA-CYC1 t
Delivery vector, TP1Ip-6pgdh-CYC1 t
Delivery vector, TPI1p-gox1705-CYC1 t
Sequences of Methylobacillus flagellatus KT gndA [Genbank: AAF34407.1], Gluconobacter oxydans 621H gox1705 [Genbank: AAW61445.1] and Bradyrhizobium japonicum USDA 110 6pgdh were codon optimized based on the codon composition of highly expressed glycolytic genes . In the case of B. japonicum, the sequence of 6pgdh was obtained by aligning its translated genomic sequence [Genbank: NC_004463.1] with the other two proteins (45 and 57 % similarity respectively). In yeast integration cassettes, the codon-optimized coding sequences of these bacterial genes were flanked by the native yeast promoter of TPI1 and the terminator of CYC1. Complete expression cassettes [Genbank: KU601575, KU601576, KU601577] were synthesized by GeneArt GmbH (Regensburg, Germany) and delivered in pMK-RQ vectors (GeneArt). After cloning in E. coli, plasmids were re-isolated and used as templates for PCR amplification of the integration cassettes. The integration cassettes TPI1p-gndA-CYC1t, TPI1p-6pgdH-CYC1t and TPI1p-gox1705-CYC1t were obtained by PCR using, respectively, primer combination 7380-7381 and plasmids pMK-RQ-gndA, pMK-RQ-6pgdH and pMK-RQ-gox1705 as templates.
A gene encoding E. coli eutE [Genbank: WP_001075673.1], codon-pair optimized for expression in S. cerevisiae  was obtained from pBOL199 by digestion with XhoI/SpeI and ligated into pAG426GPD-ccdB (Addgene, Cambridge, MA, USA), yielding the multi-copy plasmid pUDE197. For integration cassette preparation, SacI/EagI-digested pRS406 (Addgene) was used as a plasmid backbone and ligated with the TDH3p-eutE-CYC1t cassette [Genbank: KU601578], which was obtained from pUDE197 by digestion with the same restriction enzymes, yielding plasmid pUDI076.
The integration cassette TDH3p-eutE-CYC1t was amplified using primers 7991 and 7992 with plasmid pUDI076 as template. These primers were designed to add 60 bp of DNA sequence at the 5′ and 3′ ends of the PCR products, corresponding to the sequences directly upstream and downstream of the open-reading frames of the targeted chromosomal genes. The TPI1p-gndA-CYC1t, TPI1p-6pgdH-CYC1t and TPI1p-gox1705-CYC1t expression cassettes were targeted to GND1 and the TDH3p-eutE-CYC1t cassette was targeted to GPD2.
Yeast transformations were performed using the lithium acetate method . Selection of mutants was performed on synthetic medium agar plates (2 % Bacto Agar, BD, Franklin Lakes, NJ)  with 20 g L−1 glucose as carbon source and with acetamide as sole nitrogen source . In each case, correct integration was verified by diagnostic PCR, using primer combinations binding outside the targeted loci as well as inside the coding sequences of the integrated cassettes (Additional file 1: Table S1). Plasmid recycling after each transformation was performed as described previously .
Strain IMK643 was obtained by markerless CRISPR/Cas9-based deletion of GND2 by co-transformation of the gRNA-expressing plasmid pUDR123 and the repair oligo nucleotides 7299–7300. The TPI1p-gndA-CYC1t, TPI1p-6pgdH-CYC1t and TPI1p-gox1705-CYC1t integration cassettes were transformed to IMK643, along with the gRNA expressing plasmid pUDR122, yielding strains IMX705, IMX706 and IMX707 respectively. Co-transformation of the pMEL11 backbone, the ALD6-targeting gRNA-expressing plasmid insert and the repair oligonucleotides 7608–7609 to strains IMX705 and IMX585 yielded strains IMX756 and IMX899 respectively, in which ALD6 was deleted without integration of a marker. Co-transformation of the pROS11 backbone, the GPD2-targeting gRNA-expressing plasmid insert and the TDH3p-eutE-CYC1t integration cassette to strains IMX756 and IMX585 yielded strains IMX817 and IMX883 respectively. Markerless deletion of GPD1 in strains IMX817 and IMX883 was performed by co-transformation of the pROS11 backbone, the GPD1-targeting gRNA-expressing plasmid insert and the repair oligo nucleotides 6967–6968, yielding strains IMX860 and IMX888 respectively.
Cultivation and media
Shake-flask cultures were grown in 500-mL flasks containing 100 mL of synthetic medium  supplemented with glucose to a final concentration of 20 g L−1 under an air atmosphere. The pH was adjusted to 6 by addition of 2 M KOH before autoclaving at 120 °C for 20 min. Glucose solutions were autoclaved separately at 110 °C for 20 min and added to the sterile flasks. Vitamin solutions  were filter sterilized and added to the sterile flasks separately. Cultures were grown at 30 °C and shaken at 200 rpm. Initial pre-culture shake flasks were inoculated from frozen stocks in each case. After 8–12 h, fresh pre-culture flasks were inoculated from the initial flasks. Cultures prepared in this way were used for shake-flask experiments or as inoculum for anaerobic bioreactor experiments. Bioreactors were inoculated from exponentially growing pre-culture flasks to an initial OD660 of 0.2–0.3. Anaerobic batch cultivations were performed in 2-L Applikon bioreactors (Applikon, Schiedam, The Netherlands) with a 1-L working volume. All anaerobic batch fermentations were performed in synthetic medium (20 g L−1 glucose), prepared as described above. Anaerobic growth media additionally contained 0.2 g L−1 sterile antifoam C (Sigma-Aldrich), ergosterol (10 mg L−1) and Tween 80 (420 mg L−1), added separately. Bioreactor cultivations were performed at 30 °C and at a stirrer speed of 800 rpm. Nitrogen gas (<10 ppm oxygen) was sparged through the cultures at 0.5 L min−1 and culture pH was maintained at 5.0 by automated addition of 2 M KOH. Bioreactors were equipped with Norprene tubing and Viton O-rings to minimize oxygen diffusion. All strains and conditions were tested in independent duplicate cultures.
Determination of optical density at 660 nm was done using a Libra S11 spectrophotometer (Biochrom, Cambridge, UK). Off-gas analysis, biomass dry weight measurements, HPLC analysis of culture supernatants and correction for ethanol evaporation in bioreactor experiments were performed as described previously . For anaerobic batch cultures, biomass concentrations were estimated from OD660 measurements, using calibration curves based of a minimum of six samples taken in mid-exponential phase for which both biomass dry weight and OD660 were measured. Yields of each fermentation were calculated from a minimum of six samples taken during the mid-exponential growth phase by plotting either biomass against substrate, ethanol against substrate, glycerol against substrate, acetate against substrate, glycerol against biomass or acetate against biomass and calculating the absolute value of the slopes of the resulting linear fits. An example of the calculations performed is given in Additional file 2: Table S2.
Cell extracts for in vitro enzyme-activity assays were prepared as described previously  from exponentially growing shake-flask cultures harvested at an OD660 between 4 and 5. Spectrophotometric assays were performed at 30 °C and conversion of NAD+/NADP+ to NADH/NADPH was monitored by measuring absorbance at 340 nm. For NAD+- or NADP+-linked 6-phosphogluconate dehydrogenase, the 1-mL assay mixture contained 50 mM Tris–HCl (pH 8.0), 5 mM MgCl2, 0.4 mM NAD+ or NADP+ and 50 or 100 μL of cell extract. Reactions were started by addition of 6-phosphogluconate to a concentration of 5 mM. Glucose-6-phosphate dehydrogenase activity was routinely measured as a quality check of the cell extracts, using an assay mix containing 50 mM Tris–HCl (pH 8.0), 5 mM MgCl2, 0.4 mM NADP+ and 50 or 100 μl of cell extract in a volume of 1 mL. The reaction was started by addition of glucose-6-phosphate to a concentration of 5 mM. NADP+-linked glucose-6-phosphate dehydrogenase activities in different cell extracts varied between 0.43 and 0.55 μmol (mg protein)−1 min−1. All assays were performed in duplicate and reaction rates were proportional to the amount of cell extract added.
Theoretical analysis of the stoichiometric impact of altering the cofactor specificity of 6-PGDH
From Eqs. 1–4, anaerobic formation of 1 g of wild-type S. cerevisiae biomass from glucose can be calculated to require 71.38 mmol ATP for biosynthesis and 11.02 mmol ATP for NAD+ regeneration and to result in the formation of 11.02 mmol glycerol g x −1 and 82.4 mmol ethanol g x −1 . This corresponds to a predicted glycerol yield on glucose of 0.104 g g−1 and an ethanol yield on glucose of 0.391 g g−1.
When the cofactor specificity of 6-phosphogluconate dehydrogenase is changed from NADP+ to NAD+, formation of NADPH in the oxidative branch of the pentose-phosphate pathway only occurs in the glucose-6-phosphate dehydrogenase reaction. As a result, only 1 mol of NADPH is formed for each mol of glucose converted via this pathway and, moreover, its formation is coupled to the formation of 1 mol of NADH (Fig. 1; bottom numbers). In this scenario, Eq. 2 should therefore be replaced by NADPH formation according to Eq. 5.
Assuming an identical ATP, NAD+ and NADPH requirement for biosynthesis of 1 g of biomass (Eq. 1) and exclusive formation of NADPH via this modified version of the oxidative pentose-phosphate pathway (Eq. 5), the flux through the pentose-phosphate pathway should, at the same specific growth rate, be twice as high in the engineered strain as in the wild type (Fig. 1). As a result, an additional 6.48 mmol g x −1 NADH are generated which, under anaerobic conditions, need to be reoxidized to NAD+ via glycerol formation (Eq. 3). The increased ATP requirement for glycerol formation also requires an increased conversion of glucose into ethanol, according to the stoichiometry shown in Eq. 4. The total amount of glucose that is required for production of 1 g of biomass in this scenario increases to 60.96 mmol g x −1 (Fig. 1). As a result, the glycerol yield on glucose is predicted to increase to 0.147 g g−1 (41 % increase relative to wild type), while the ethanol yield on glucose is predicted to decrease to 0.373 g g−1 (5 % decrease relative to wild type). Furthermore, the biomass yield on glucose is predicted to decrease to 0.091 gx g−1 (12 % decrease relative to wild type) in the engineered strain. This corresponds to an increase of 59 % on the glycerol formed per g of biomass relative to wild type.
Characterization of S. cerevisiae strains expressing NAD+-dependent 6-PGDH
Maximum specific growth rates in shake-flask cultures and ratio of NAD+- and NADP+-linked 6-phosphogluconate dehydrogenase activity in cell extracts of a reference S. cerevisiae strain with native NADP+-dependent 6-phosphogluconate dehydrogenase (IMX585) and three strains expressing different heterologous NAD+-dependent 6-phosphogluconate dehydrogenases (IMX705-707)
NAD+/NADP+ linked activity ratio
0.38 ± 0.01
0.36 ± 0.00
46 ± 10
0.28 ± 0.01
5 ± 0.2
0.36 ± 0.00
11 ± 0.5
Maximum specific growth rate (μ), yields (Y) of glycerol, biomass and ethanol on glucose and the ratios of glycerol and acetate formation to biomass formation in anaerobic bioreactor batch cultures of S. cerevisiae strains IMX585, IMX705, IMX899 and IMX756
GND1 GND2 ald6Δ
gnd2Δ gnd1::gndA ald6Δ
0.32 ± 0.00
0.30 ± 0.01
0.29 ± 0.01
0.26 ± 0.01
Y glycerol/glucose (g g−1)
0.105 ± 0.000
0.121 ± 0.001
0.106 ± 0.000
0.146 ± 0.000
Y biomass/glucose (gx g−1)
0.094 ± 0.004
0.087 ± 0.002
0.088 ± 0.001
0.083 ± 0.002
Y EtOH/glucose (g g−1)
0.372 ± 0.001
0.379 ± 0.001
0.386 ± 0.000
0.374 ± 0.002
Ratio glycerol formed/biomass (mmol g x −1 )
12.19 ± 0.44
15.14 ± 0.22
12.83 ± 0.39
18.90 ± 0.56
Ratio acetate formed/biomass (mmol g x −1 )
1.50 ± 0.03
1.63 ± 0.02
Anaerobic cultures of strain IMX705 (gnd2Δ gnd1::gndA) showed a ca. 9 % higher production of extracellular acetate than those of the reference strain IMX585 (Table 4). Acetate can be formed via cytosolic NADP+-dependent acetaldehyde dehydrogenase, which is encoded by ALD6 and provides an alternative route of cytosolic NADPH formation . NADPH formation through Ald6 is not desirable in an ethanol producing strain, since it decreases the impact of the cofactor switch of 6-PGDH and results in the production of acetate instead of ethanol. To eliminate this alternative NADPH-forming route, ALD6 was deleted in strain IMX705, yielding strain IMX756 (gnd2Δ gnd1::gndA ald6Δ). To distinguish between the impact of ALD6 deletion alone and in combination with NAD+-dependent 6-PGDH, ALD6 was also deleted in strain IMX585, yielding strain IMX899 (GND1 GND2 ald6Δ). Strains IMX899 (GND1 GND2 ald6Δ) and IMX756 (gnd2Δ gnd1::gndA ald6Δ) were then characterized in anaerobic bioreactor experiments under the same conditions as the previous experiments with their parental strains IMX585 and IMX705 (Table 4; Fig. 3c, d). Deletion of ALD6 in strains IMX899 (GND1 GND2 ald6Δ) and IMX756 (gnd2Δ gnd1::gndA ald6Δ) resulted in slightly lower specific growth rates (90 % and 81 %, respectively) than those observed in the case of the reference strain IMX585 (GND1 GND2). The additional growth rate decrease of strain IMX756 could be an indication of a limited capacity of NADPH formation in the absence of both Ald6 and NADP+-dependent 6-PGDH.
Inactivation of ALD6 resulted in a strong decrease in the production of acetate during the early stages of the anaerobic cultures, and acetate concentrations even dropped to below detection level during the later stages of cultivation in both IMX899 (GND1 GND2 ald6Δ) and IMX756 (gnd2Δ gnd1::gndA ald6Δ) (Fig. 3). In strain IMX899, the deletion of ALD6 resulted in a glycerol production of 12.83 mmol g x −1 compared to 12.19 mmol g x −1 for IMX585 (Table 4). This small difference suggested that, in the presence of native 6-PGDH, the contribution of Ald6 to NADPH formation is limited in this strain background. However, in combination with gndA overexpression and deletion of GND1 and GND2, deletion of ALD6 resulted in a 55 % increase of the glycerol formation, from 12.19 mmol g x −1 in IMX585 to 18.90 mmol g x −1 in strain IMX756 (Table 4), which closely corresponds to the theoretically predicted 59 % increase. The biomass yield of strain IMX756 (gnd2Δ gnd1::gndA ald6Δ) was 13 % lower than the reference strain IMX585 (GND1 GND2), as compared to a theoretically predicted 12 % decrease. The corresponding glycerol yield on glucose of strain IMX756 was 39 % higher (0.146 g g−1 compared to 0.105 g g−1) than the glycerol yield of the GND1 GND2 reference strain IMX585 (Table 4).
Theoretical analysis of the impact of changing the cofactor specificity of 6-PGDH in an acetate-reducing strain
Changing the cofactor specificity of 6-PGDH from NADP+ to NAD+ (Eq. 5) in an acetate-reducing strain should result in an increase in the acetate requirement to 8.75 mmol g x −1 (Fig. 4; bottom numbers). This corresponds to an increase of 59 % relative to the strain expressing the native enzyme. As reduction of acetate to ethanol requires ATP (Eq. 6), the requirement for glucose in this scenario increases to 52.21 mmol g x −1 , resulting in the formation of 97.63 mmol g x −1 ethanol. This scenario, therefore, results in an increase in acetate consumption per g of consumed glucose to 0.056 g, which corresponds to an increase of 47 % relative to an acetate-reducing strain expressing the native, NADP+-dependent 6-PGDH. Additionally, the apparent ethanol yield on glucose is predicted to further increase, by an additional 3 %, to 0.478 g g−1.
Physiological impact of gndA expression and ALD6 deletion in an acetylating acetaldehyde dehydrogenase expressing strain
Maximum specific growth rate (μ), yields (Y) of glycerol, biomass and ethanol on glucose and the ratios of glycerol production to biomass formation and acetate consumption to glucose consumption and biomass formation in anaerobic bioreactor batch cultures of S. cerevisiae strains IMX585, IMX888 and IMX860
GND1 GND2 GPD1 GPD2
GND1 GND2 gpd2::eutE gpd1Δ
gnd2Δ gnd1::gndA ald6Δ gpd2::eutE gpd1Δ
0.28 ± 0.01
0.26 ± 0.01
0.20 ± 0.01
Y glycerol/glucose (g g−1)
0.060 ± 0.000
Y biomass/glucose (gx g−1)
0.076 ± 0.003
0.075 ± 0.000
0.077 ± 0.000
Y EtOH/glucose (g g−1)
0.433 ± 0.001
0.474 ± 0.001
0.489 ± 0.000
Ratio glycerol produced/biomass (mmol g x −1 )
8.50 ± 0.04
Ratio acetate consumed/biomass (mmol g x −1 )
2.44 ± 0.10
6.92 ± 0.12
8.90 ± 0.04
Ratio acetate consumed/glucose (g g−1)
0.011 ± 0.00
0.032 ± 0.00
0.042 ± 0.00
The maximum specific growth rate of the acetate reducing strain with native 6-PGDH (IMX888) in the presence of acetate was 93 % of that of the reference strain IMX585 (Table 5). This represents a significant improvement in the specific growth rate relative to what was previously reported for a gpd1Δ gpd2Δ strain expressing mhpF from E. coli which, in the same genetic background, displayed only half the growth rate of a GPD1 GPD2 reference strain . This difference indicates that, in the previous study, the in vivo activity of the heterologous acetylating acetaldehyde dehydrogenase limited the rate of acetate reduction and, thereby, the specific growth rate. The apparent ethanol yield on glucose (corrected for ethanol evaporation but not for use of acetate as a substrate for ethanol formation) of strain IMX888 (GND1 GND2 gpd1Δ gpd2::eutE) was 0.474 g g−1, compared to 0.433 g g−1 of the reference strain IMX585 (GND1 GND2 GPD1 GPD2) (Table 5). This corresponds to an increase of 9 % and is consistent with a previous report on a gpd1Δ gpd2Δ strain that overexpressed mhpF . Strain IMX888 (GND1 GND2 gpd1Δ gpd2::eutE) showed an acetate consumption of 6.92 mmol g x −1 (Table 5). Corrected for the acetate-consumption of strain IMX585 as described above, it follows that 4.48 mmol g x −1 acetate were reduced to ethanol via the EutE-dependent pathway. The corresponding regeneration of 8.96 mmol NAD+ g x −1 is very close to the regeneration of 8.50 mmol NAD+ g x −1 via glycerol production of strain IMX585 (Table 5).
The NAD+-dependent 6-PGDH-expressing strain IMX860 (gnd2Δ gnd1::gndA ald6Δ gpd1Δ gpd2::eutE) showed a growth rate that was 29 % lower than that of the reference strain IMX585 (GND1 GND2 GPD1 GPD2) (Table 5). This difference in growth rate increased the overall fermentation time by ca. 5 h (Fig. 5). The acetate consumption of strain IMX860 (gnd2Δ gnd1::gndA ald6Δ gpd1Δ gpd2::eutE) was 8.9 mmol g x −1 (Table 5). Corrected for the acetate consumption of IMX585, this corresponds to the regeneration of 12.92 mmol NADH g x −1 via reduction of 6.46 mmol g x −1 acetate to ethanol via the EutE-dependent pathway. These calculations indicate that increased NADH generation via NAD+-dependent 6-PGDH resulted in a 44 % increase in the EutE-dependent acetate consumption per g biomass of strain IMX860, compared to the native 6-PGDH expressing strain IMX888. In regard to the overall fermentation performance, strain IMX860 consumed 0.042 g acetate per g of consumed glucose, which is 31 % higher than the observed consumption of strain IMX888 (GND1 GND2 gpd1Δ gpd2::eutE) (Fig. 5; Table 5). Furthermore, strain IMX860 (gnd2Δ gnd1::gndA ald6Δ gpd1Δ gpd2::eutE) showed an apparent ethanol yield on glucose of 0.489 g g−1, which corresponded to an increase of 3 % compared to strain IMX888 (GND1 GND2 gpd1Δ gpd2::eutE) and an increase of 13 % compared to strain IMX585 under the same conditions (Table 5). In comparison to IMX585 in the absence of (added) acetate, the combined effects of weak-acid uncoupling, acetate-consumption and the redox-cofactor of 6-PGDH in IMX860 increased the (apparent) ethanol yield on glucose by 32 % from 0.372 to 0.489 g g−1.
The cofactor switch from NADP+-dependent 6-PGDH to an NAD+-dependent enzyme, in combination with deletion of ALD6, elimination of glycerol formation and heterologous expression of acetylating acetaldehyde dehydrogenase, resulted in a strain with significantly increased acetic acid consumption per g of biomass formed in synthetic media. However, even when corrected for acetate consumption independent of acetylating acetaldehyde dehydrogenase, the experimentally observed acetate consumption increase of 44 % was lower than the theoretically predicted 59 %. This deviation can, for instance, be caused by differences in biomass composition due to differences between strain backgrounds and/or their specific growth rates (specific growth rate is known to affect RNA and protein content ), or by suboptimal enzyme kinetics due to lower than predicted in vivo activity/affinity of GndA with NAD+ instead of NADP+, which could result in some NADPH formation. One clear possibility for further improvement is the maximum specific growth rate of the acetate-reducing strains. In strain IMX860 (gnd2Δ gnd1::gndA ald6Δ gpd1Δ gpd2::eutE) the specific growth rate was 29 % lower than that of the reference strain IMX585 under the same conditions. The superior growth rates of strains expressing the EutE acetylating acetaldehyde dehydrogenase, instead of the previously used MhpF , identifies the in vivo capacity of this enzyme as a relevant target for further engineering studies, especially in strains with an increased requirement for NADH-regeneration. In addition to a systematic evaluation of alternative acetylating acetaldehyde-dehydrogenase genes, the copy number of the corresponding expression cassettes can also vary. Alternatively, a limited in vivo capacity of NAD+-dependent 6-PGDH, for which only 3 candidate genes were screened, and/or of the non-oxidative pentose-phosphate pathway may be responsible for the suboptimal growth rates of the engineered strains. As an alternative approach, cofactor engineering of the native NADP+-dependent glucose-6-phosphate dehydrogenase  might be considered. The stoichiometric impact of such an intervention is expected to be identical to that of the strategy presented in this study.
Recently, an alternative metabolic engineering strategy to increase the reduction of acetate to ethanol was described . This alternative strategy is based on introduction of a heterologous NADPH-dependent alcohol dehydrogenase in combination with overexpression of ZWF1 and/or ACS2. In contrast to the strategy described in the present study, this alternative strategy is not dependent on NADH generation in biosynthesis. The absence of a stoichiometric coupling to growth potentially provides more flexibility in acetate reduction but might also lead to cells that are less stable during long-term cultivation, since mutational loss of either ZWF1 overexpression or NADPH-dependent alcohol dehydrogenase provides a benefit for the cells. Further research is required to study how these two strategies, which can in principle be combined, can be used to maximize acetic-acid detoxification and optimization of ethanol yields in lignocellulosic hydrolysates. Such research should also address the question of how changes in NADPH formation affect cellular robustness in lignocellulosic hydrolysates, since NADPH can play a key role in the reductive detoxification of, for example, 2-furaldehyde (furfural) and 5-hydroxymethyl furaldehyde (HMF) to the corresponding less toxic alcohols [10, 17, 26]. Although the strains in this study have a gpd1Δ gpd2Δ (Gpd−) genotype, which can affect strain performance in industrial fermentations that are operated at high osmotic pressures [1, 3], this phenotype can be overcome by additional metabolic engineering steps, such as expression of alternative compatible solutes , tuning of expression of Gpd1/2 [25, 37], or by evolutionary engineering of growth in high osmolarity media .
The possible applications of the cofactor engineering strategy presented in this study extend beyond increasing acetate consumption in second-generation ethanol production. Altering the balance between glycerol and ethanol production is, for example, of interest to wine fermentation, in which a shift of carbon away from ethanol production is desirable during fermentation of grapes with high sugar content [31, 52]. Several previous studies have investigated increased glycerol production as a means to decrease the ethanol content of the wine [34, 61, 65] without negatively affecting its organoleptic properties . A benefit of the strategy presented in this work is that formation of the NADH required for additional glycerol formation is coupled to carbon dioxide production rather than to increased formation of organic products such as acetate, pyruvate or acetaldehyde, which negatively affect wine quality [5, 12, 52]. However, it must be noted that, in spite of an increased glycerol formation, the ethanol yield on glucose in our study did not decrease in a strain containing NAD+-dependent 6-phosphogluconate dehydrogenase, caused by a larger than predicted decrease in the biomass yield. Analysis of the applicability in wine fermentation, therefore, requires a careful analysis of product formation under actual wine fermentation conditions. In general, this novel approach can be used to improve production of compounds that are more reduced than glucose in glucose-based industrial processes using S. cerevisiae. Expression of a NAD+-dependent 6-PGDH can also be applied in metabolic engineering strategies for production of compounds that require pentose-phosphate pathway derived precursors, such as for example erythrose-4-phosphate for 2-phenylethanol  or flavonoid production , but that do not require (all) the accompanying NADPH formation.
This work demonstrates an efficient and versatile strategy to increase cytosolic NADH generation in S. cerevisiae by engineering the cofactor specificity of the oxidative part of the pentose-phosphate pathway. The strategy was successfully applied to the generation of a strain that was able to reduce more acetate and produce more ethanol than a non-engineered, acetate-reducing reference strain.
IP, JTP and AJAvM designed experiments and wrote the text. IP selected the heterologous genes, constructed yeast strains and performed enzymatic activity determinations and fermentations. MvD constructed yeast strains and performed fermentations. APAD constructed yeast strains, performed fermentations and carried out enzymatic activity determinations. BM selected and screened the acetylating acetaldehyde dehydrogenase. All authors read and approved the final manuscript.
This project is part of a collaboration between the Delft University of Technology and DSM. We thank Jean-Marc Daran, Pilar de la Torre and Paul Klaassen for advice on molecular biology, Matthijs Niemeijer and Erik de Hulster for advice on fermentations, Marcel van der Broek for assistance with 6-PGDH sequence data screening, Marijke Luttik for advice on enzymatic activity determinations and Susan Weening for pre-screening of acetylating acetaldehyde dehydrogenases.
Availability of data and materials
IP, JTP and AJAvM are inventors on a patent application related to the content of this work. The PhD project of IP is funded by DSM Bio-based Products & Services B.V. (Delft, The Netherlands).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Albertyn J, Hohmann S, Thevelein JM, Prior BA. GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Mol Cell Biol. 1994;14:4135–44.View ArticleGoogle Scholar
- Bellissimi E, van Dijken JP, Pronk JT, van Maris AJA. Effects of acetic acid on the kinetics of xylose fermentation by an engineered, xylose-isomerase-based Saccharomyces cerevisiae strain. FEMS Yeast Res. 2009;9:358–64.View ArticleGoogle Scholar
- Blomberg A, Adler L. Roles of glycerol and glycerol-3-phosphate dehydrogenase (NAD+) in acquired osmotolerance of Saccharomyces cerevisiae. J Bacteriol. 1989;171:1087–92.View ArticleGoogle Scholar
- Bruinenberg PM, van Dijken JP, Scheffers WA. A theoretical analysis of NADPH production and consumption in yeasts. J Gen Microbiol. 1983;129:953–64.Google Scholar
- Cambon B, Monteil V, Remize F, Camarasa C, Dequin S. Effects of GPD1 overexpression in Saccharomyces cerevisiae commercial wine yeast strains lacking ALD6 genes. Appl Environ Microb. 2006;72:4688–94.View ArticleGoogle Scholar
- Celton M, Goelzer A, Camarasa C, Fromion V, Dequin S. A constraint-based model analysis of the metabolic consequences of increased NADPH oxidation in Saccharomyces cerevisiae. Metab Eng. 2012;14:366–79.View ArticleGoogle Scholar
- Celton M, Sanchez I, Goelzer A, Fromion V, Camarasa C, Dequin S. A comparative transcriptomic, fluxomic and metabolomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation. BMC Genom. 2012;13:317.View ArticleGoogle Scholar
- Chistoserdova L, Gomelsky L, Vorholt JA, Gomelsky M, Tsygankov YD, Lidstrom ME. Analysis of two formaldehyde oxidation pathways in Methylobacillus flagellatus KT, a ribulose monophosphate cycle methylotroph. Microbiology. 2000;146:233–8.View ArticleGoogle Scholar
- Ciriacy M. Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. Mutat Res-Fund Mol M. 1975;29:315–25.View ArticleGoogle Scholar
- Cunha JT, Aguiar TQ, Romaní A, Oliveira C, Domingues L. Contribution of PRS3, RPB4 and ZWF1 to the resistance of industrial Saccharomyces cerevisiae CCUG53310 and PE-2 strains to lignocellulosic hydrolysate-derived inhibitors. Bioresour Technol. 2015;191:7–16.View ArticleGoogle Scholar
- DiCarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res. 2013;41:4336–43.View ArticleGoogle Scholar
- Eglinton JM, Heinrich AJ, Pollnitz AP, Langridge P, Henschke PA, de Barros Lopes M. Decreasing acetic acid accumulation by a glycerol overproducing strain of Saccharomyces cerevisiae by deleting the ALD6 aldehyde dehydrogenase gene. Yeast. 2002;19:295–301.View ArticleGoogle Scholar
- Entian KD, Kötter P. Yeast genetic strain and plasmid collections. Method Microbiol. 2007;36:629–66.View ArticleGoogle Scholar
- Etschmann M, Bluemke W, Sell D, Schrader J. Biotechnological production of 2-phenylethanol. Appl Microbiol Biotechnol. 2002;59:1–8.View ArticleGoogle Scholar
- Flikweert MT, de Swaaf M, van Dijken JP, Pronk JT. Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae. FEMS Microbiol Lett. 1999;174:73–9.View ArticleGoogle Scholar
- Gietz RD, Woods RA. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol. 2002;350:87–96.View ArticleGoogle Scholar
- Gorsich SW, Dien BS, Nichols NN, Slininger PJ, Liu ZL, Skory CD. Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPE1, and TKL1 in Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2006;71:339–49.View ArticleGoogle Scholar
- Grabowska D, Chelstowska A. The ALD6 gene product is indispensable for providing NADPH in yeast cells lacking glucose-6-phosphate dehydrogenase activity. J Biol Chem. 2003;278:13984–8.View ArticleGoogle Scholar
- Guadalupe-Medina V, Metz B, Oud B, van der Graaf CM, Mans R, Pronk JT, van Maris AJA. Evolutionary engineering of a glycerol-3-phosphate dehydrogenase-negative, acetate-reducing Saccharomyces cerevisiae strain enables anaerobic growth at high glucose concentrations. Microb Biotechnol. 2014;7:44–53.View ArticleGoogle Scholar
- Guadalupe-Medina V, Almering MJH, van Maris AJA, Pronk JT. Elimination of glycerol production in anaerobic cultures of a Saccharomyces cerevisiae strain engineered to use acetic acid as an electron acceptor. Appl Environ Microb. 2010;76:190–5.View ArticleGoogle Scholar
- Guadalupe-Medina V, Wisselink H, Luttik M, de Hulster E, Daran J-M, Pronk JT, van Maris AJA. Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeast. Biotechnol Biofuels. 2013;6:125.View ArticleGoogle Scholar
- Hahn-Hägerdal B, Karhumaa K, Fonseca C, Spencer-Martins I, Gorwa-Grauslund MF. Towards industrial pentose-fermenting yeast strains. Appl Microbiol Biotechnol. 2007;74:937–53.View ArticleGoogle Scholar
- Hasunuma T, Sanda T, Yamada R, Yoshimura K, Ishii J, Kondo A. Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae. Microb Cell Fact. 2011;10:2.View ArticleGoogle Scholar
- Henningsen BM, Hon S, Covalla SF, Sonu C, Argyros DA, Barrett TF, Wiswall E, Froehlich AC, Zelle RM. Increasing anaerobic acetate consumption and ethanol yield in Saccharomyces cerevisiae with NADPH-specific alcohol dehydrogenase. Appl Environ Microbiol. 2015;81:8108–17.View ArticleGoogle Scholar
- Hubmann G, Guillouet S, Nevoigt E. Gpd1 and Gpd2 fine-tuning for sustainable reduction of glycerol formation in Saccharomyces cerevisiae. Appl Environ Microbiol. 2011;77:5857–67.View ArticleGoogle Scholar
- Jeppsson M, Johansson B, Jensen PR, Hahn-Hägerdal B, Gorwa-Grauslund MF. The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains. Yeast. 2003;20:1263–72.View ArticleGoogle Scholar
- Keating JD, Panganiban C, Mansfield SD. Tolerance and adaptation of ethanologenic yeasts to lignocellulosic inhibitory compounds. Biotechnol Bioeng. 2006;93:1196–206.View ArticleGoogle Scholar
- Klinke HB, Thomsen AB, Ahring BK. Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass. Appl Microbiol Biotechnol. 2004;66:10–26.View ArticleGoogle Scholar
- Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch D, van Maris AJA, Pronk JT, Daran J-M. De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae. Microb Cell Fact. 2012;11:155.View ArticleGoogle Scholar
- Kozak BU, van Rossum HM, Benjamin KR, Wu L, Daran J-M, Pronk JT, van Maris AJA. Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases by alternative pathways for cytosolic acetyl-CoA synthesis. Metab Eng. 2014;21:46–59.View ArticleGoogle Scholar
- Kutyna DR, Varela C, Henschke PA, Chambers PJ, Stanley GA. Microbiological approaches to lowering ethanol concentration in wine. Trends Food Sci Tech. 2010;21:293–302.View ArticleGoogle Scholar
- Larsson S, Palmqvist E, Hahn-Hägerdal B, Tengborg C, Stenberg K, Zacchi G, Nilvebrant NO. The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microb Tech. 1999;24:151–9.View ArticleGoogle Scholar
- Mans R, van Rossum HM, Wijsman M, Backx A, Kuijpers NG, van den Broek M, Daran-Lapujade P, Pronk JT, van Maris AJA, Daran J-M. CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae. FEMS Yeast Res. 2015;15:fov004.View ArticleGoogle Scholar
- Michnick S, Roustan JL, Remize F, Barre P, Dequin S. Modulation of glycerol and ethanol yields during alcoholic fermentation in Saccharomyces cerevisiae strains overexpressed or disrupted for GPD1 encoding glycerol 3-phosphate dehydrogenase. Yeast. 1997;13:783–93.View ArticleGoogle Scholar
- Mira N, Palma M, Guerreiro J, Sa-Correia I. Genome-wide identification of Saccharomyces cerevisiae genes required for tolerance to acetic acid. Microb Cell Fact. 2010;9:79.View ArticleGoogle Scholar
- Müller UM, Wu L, Raamsdonk LM, Winkler AA. Acetyl-coa producing enzymes in yeast. PCT/EP2008/059119(WO2009013159 A2). 30-9-2010.Google Scholar
- Nevoigt E, Kohnke J, Fischer CR, Alper H, Stahl U, Stephanopoulos G. Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae. Appl Environ Microbiol. 2006;72:5266–73.View ArticleGoogle Scholar
- Nielsen J, Larsson C, van Maris AJA, Pronk JT. Metabolic engineering of yeast for production of fuels and chemicals. Curr Opin Biotechnol. 2013;24:398–404.View ArticleGoogle Scholar
- Nijkamp JF, van den Broek M, Datema E, de Kok S, Bosman L, Luttik MA, Daran-Lapujade P, Vongsangnak W, Nielsen J, Heijne WHM, Klaassen P, Paddon CJ, Platt D, Kötter P, van Ham RC, Reinders MJT, Pronk JT, de Ridder D, Daran J-M. De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology. Microb Cell Fact. 2012;11:36.View ArticleGoogle Scholar
- Noble AC, Bursick GF. The contribution of glycerol to perceived viscosity and sweetness in white wine. Am J Enol Viticult. 1984;35:110–2.Google Scholar
- Nogae I, Johnston M. Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase. Gene. 1990;96:161–9.View ArticleGoogle Scholar
- Orij R, Urbanus M, Vizeacoumar F, Giaever G, Boone C, Nislow C, Brul S, Smits G. Genome-wide analysis of intracellular pH reveals quantitative control of cell division rate by pHc in Saccharomyces cerevisiae. Genome Biol. 2012;13:R80.View ArticleGoogle Scholar
- Palmqvist E, Hahn-Hägerdal B. Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification. Bioresour Technol. 2000;74:17–24.View ArticleGoogle Scholar
- Palmqvist E, Hahn-Hägerdal B. Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour Technol. 2000;74:25–33.View ArticleGoogle Scholar
- Pampulha ME, Loureiro-Dias MC. Activity of glycolytic enzymes of Saccharomyces cerevisiae in the presence of acetic acid. Appl Microbiol Biotechnol. 1990;34:375–80.View ArticleGoogle Scholar
- Pampulha ME, Loureiro-Dias MC. Energetics of the effect of acetic acid on growth of Saccharomyces cerevisiae. FEMS Microbiol Lett. 2000;184:69–72.View ArticleGoogle Scholar
- Parawira W, Tekere M. Biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review. Crit Rev Biotechnol. 2010;31:20–31.View ArticleGoogle Scholar
- Rauch B, Pahlke J, Schweiger P, Deppenmeier U. Characterization of enzymes involved in the central metabolism of Gluconobacter oxydans. Appl Microbiol Biotechnol. 2010;88:711–8.View ArticleGoogle Scholar
- Roubos JA, van Noel N, Peij VNNM. A method for achieving improved polypeptide expression. PCT/EP2007/055943(WO2008000632 A1). 3-1-2008.Google Scholar
- Saint-Prix F, Bönquist L, Dequin S. Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+ -dependent Ald6p and Ald5p isoforms play a major role in acetate formation. Microbiology. 2004;150:2209–20.View ArticleGoogle Scholar
- Sanda T, Hasunuma T, Matsuda F, Kondo A. Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids. Bioresour Technol. 2011;102:7917–24.View ArticleGoogle Scholar
- Schmidtke LM, Blackman JW, Agboola SO. Production technologies for reduced alcoholic wines. J Food Sci. 2012;77:R25–41.View ArticleGoogle Scholar
- Shen B, Hohmann S, Jensen RG, Bohnert H. Roles of sugar alcohols in osmotic stress adaptation. Replacement of glycerol by mannitol and sorbitol in yeast. Plant Physiol. 1999;121:45–52.View ArticleGoogle Scholar
- Sinha A, Maitra PK. Induction of specific enzymes of the oxidative pentose phosphate pathway by glucono-δ-lactone in Saccharomyces cerevisiae. J Gen Microbiol. 1992;138:1865–73.View ArticleGoogle Scholar
- Smith J, van Rensburg E, Gorgens J. Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiae harbouring xylose isomerase. BMC Biotechnol. 2014;14:41.View ArticleGoogle Scholar
- Solis-Escalante D, Kuijpers NGA, Bongaerts N, Bolat I, Bosman L, Pronk JT, Daran J-M, Daran-Lapujade P. amdSYM, a new dominant recyclable marker cassette for Saccharomyces cerevisiae. FEMS Yeast Res. 2013;13:126–39.View ArticleGoogle Scholar
- Sosa-Saavedra F, León-Barrios M, Pérez-Galdona R. Pentose phosphate pathway as the main route for hexose catabolism in Bradyrhizobium sp. lacking Entner-Doudoroff pathway. A role for NAD+-dependent 6-phosphogluconate dehydrogenase (decarboxylating). Soil Biol Biochem. 2001;33:339–43.View ArticleGoogle Scholar
- Swinnen S, Fernández-Niño M, González-Ramos D, van Maris AJA, Nevoigt E. The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance in Saccharomyces cerevisiae. FEMS Yeast Res. 2014;14:642–53.View ArticleGoogle Scholar
- Taherzadeh MJ, Karimi K. Chapter 12—fermentation inhibitors in ethanol processes and different strategies to reduce their effects. In: Gnansounou APL, editor. Biofuels. Amsterdam: Academic Press; 2011. p. 287–311.View ArticleGoogle Scholar
- Thomas KC, Hynes SH, Ingledew WM. Influence of medium buffering capacity on inhibition of Saccharomyces cerevisiae growth by acetic and lactic acids. Appl Environ Microbiol. 2002;68:1616–23.View ArticleGoogle Scholar
- Tilloy V, Ortiz-Julien A, Dequin S. Reduction of ethanol yield and improvement of glycerol formation by adaptive evolution of the wine yeast Saccharomyces cerevisiae under hyperosmotic conditions. Appl Environ Microbiol. 2014;80:2623–32.View ArticleGoogle Scholar
- van den Berg MA, de Jong-Gubbels P, Kortland CJ, van Dijken JP, Pronk JT, Steensma HY. The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation. J Biol Chem. 1996;271:28953–9.View ArticleGoogle Scholar
- van Dijken JP, Scheffers WA. Redox balances in the metabolism of sugars by yeasts. FEMS Microbiol Lett. 1986;32:199–224.View ArticleGoogle Scholar
- van Maris AJA, Abbott DA, Bellissimi E, van den Brink J, Kuyper M, Luttik MA, Wisselink HW, Scheffers WA, van Dijken JP, Pronk JT. Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status. Antonie Van Leeuwenhoek. 2006;90:391–418.View ArticleGoogle Scholar
- Varela C, Kutyna DR, Solomon MR, Black CA, Borneman A, Henschke PA, Pretorius IS, Chambers PJ. Evaluation of gene modification strategies for the development of low-alcohol-wine yeasts. Appl Environ Microbiol. 2012;78:6068–77.View ArticleGoogle Scholar
- Verduyn C, Postma E, Scheffers WA, van Dijken JP. Energetics of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. J Gen Microbiol. 1990;136:405–12.View ArticleGoogle Scholar
- Verduyn C, Postma E, Scheffers WA, van Dijken JP. Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast. 1992;8:501–17.View ArticleGoogle Scholar
- Verduyn C, Postma E, Scheffers WA, van Dijken JP. Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. J Gen Microbiol. 1990;136:395–403.View ArticleGoogle Scholar
- Wiedemann B, Boles E. Codon-optimized bacterial genes improve l-arabinose fermentation in recombinant Saccharomyces cerevisiae. Appl Environ Microbiol. 2008;74:2043–50.View ArticleGoogle Scholar
- Wright J, Bellissimi E, de Hulster E, Wagner A, Pronk JT, van Maris AJA. Batch and continuous culture-based selection strategies for acetic acid tolerance in xylose-fermenting Saccharomyces cerevisiae. FEMS Yeast Res. 2011;11:299–306.View ArticleGoogle Scholar