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Table 3 Maximum specific growth rates in shake-flask cultures and ratio of NAD+- and NADP+-linked 6-phosphogluconate dehydrogenase activity in cell extracts of a reference S. cerevisiae strain with native NADP+-dependent 6-phosphogluconate dehydrogenase (IMX585) and three strains expressing different heterologous NAD+-dependent 6-phosphogluconate dehydrogenases (IMX705-707)

From: Improving ethanol yield in acetate-reducing Saccharomyces cerevisiae by cofactor engineering of 6-phosphogluconate dehydrogenase and deletion of ALD6

Strain

Relevant genotype

μ (h−1)

NAD+/NADP+ linked activity ratio

IMX585

GND1 GND2

0.38 ± 0.01

<0.01

IMX705

gnd2Δ gnd1::gndA

0.36 ± 0.00

46 ± 10

IMX706

gnd2Δ gnd1::6pgdh

0.28 ± 0.01

5 ± 0.2

IMX707

gnd2Δ gnd1::gox1705

0.36 ± 0.00

11 ± 0.5

  1. Shake-flask cultures (initial pH 6) were grown on synthetic medium containing 20 g L−1 glucose under an air atmosphere and cell extracts were prepared from exponentially growing cultures. Values represent the average and mean deviation of data from independent duplicate cultures