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Table 3 Maximum specific growth rates in shake-flask cultures and ratio of NAD+- and NADP+-linked 6-phosphogluconate dehydrogenase activity in cell extracts of a reference S. cerevisiae strain with native NADP+-dependent 6-phosphogluconate dehydrogenase (IMX585) and three strains expressing different heterologous NAD+-dependent 6-phosphogluconate dehydrogenases (IMX705-707)

From: Improving ethanol yield in acetate-reducing Saccharomyces cerevisiae by cofactor engineering of 6-phosphogluconate dehydrogenase and deletion of ALD6

Strain Relevant genotype μ (h−1) NAD+/NADP+ linked activity ratio
IMX585 GND1 GND2 0.38 ± 0.01 <0.01
IMX705 gnd2Δ gnd1::gndA 0.36 ± 0.00 46 ± 10
IMX706 gnd2Δ gnd1::6pgdh 0.28 ± 0.01 5 ± 0.2
IMX707 gnd2Δ gnd1::gox1705 0.36 ± 0.00 11 ± 0.5
  1. Shake-flask cultures (initial pH 6) were grown on synthetic medium containing 20 g L−1 glucose under an air atmosphere and cell extracts were prepared from exponentially growing cultures. Values represent the average and mean deviation of data from independent duplicate cultures