Skip to main content

Table 4 Maximum specific growth rate (μ), yields (Y) of glycerol, biomass and ethanol on glucose and the ratios of glycerol and acetate formation to biomass formation in anaerobic bioreactor batch cultures of S. cerevisiae strains IMX585, IMX705, IMX899 and IMX756

From: Improving ethanol yield in acetate-reducing Saccharomyces cerevisiae by cofactor engineering of 6-phosphogluconate dehydrogenase and deletion of ALD6

Strain IMX585 IMX705 IMX899 IMX756
Relevant genotype GND1 GND2 gnd2Δ gnd1::gndA GND1 GND2 ald6Δ gnd2Δ gnd1::gndA ald6Δ
μ (h−1) 0.32 ± 0.00 0.30 ± 0.01 0.29 ± 0.01 0.26 ± 0.01
Y glycerol/glucose (g g−1) 0.105 ± 0.000 0.121 ± 0.001 0.106 ± 0.000 0.146 ± 0.000
Y biomass/glucose (gx g−1) 0.094 ± 0.004 0.087 ± 0.002 0.088 ± 0.001 0.083 ± 0.002
Y EtOH/glucose (g g−1) 0.372 ± 0.001 0.379 ± 0.001 0.386 ± 0.000 0.374 ± 0.002
Ratio glycerol formed/biomass (mmol g −1x ) 12.19 ± 0.44 15.14 ± 0.22 12.83 ± 0.39 18.90 ± 0.56
Ratio acetate formed/biomass (mmol g −1x ) 1.50 ± 0.03 1.63 ± 0.02 <0.05 <0.05
  1. Cultures were grown on synthetic medium containing 20 g L−1 glucose (pH 5). Yields and ratios were calculated from the exponential growth phase. The ethanol yield on glucose was corrected for evaporation. Values represent average and mean deviation of data from independent duplicate cultures. Carbon recovery in all fermentations was between 95 and 100 %