Table 5 Maximum specific growth rate (μ), yields (Y) of glycerol, biomass and ethanol on glucose and the ratios of glycerol production to biomass formation and acetate consumption to glucose consumption and biomass formation in anaerobic bioreactor batch cultures of S. cerevisiae strains IMX585, IMX888 and IMX860

Relevant genotype

GND1 GND2 GPD1 GPD2

GND1 GND2 gpd2::eutE gpd1Δ

gnd2Δ gnd1::gndA ald6Δ gpd2::eutE gpd1Δ

μ (h⁻¹)

0.28 ± 0.01

0.26 ± 0.01

0.20 ± 0.01

Y glycerol/glucose (g g⁻¹)

0.060 ± 0.000

<0.001

<0.001

Y biomass/glucose (g_x g⁻¹)

0.076 ± 0.003

0.075 ± 0.000

0.077 ± 0.000

Y EtOH/glucose (g g⁻¹)

0.433 ± 0.001

0.474 ± 0.001

0.489 ± 0.000

Ratio glycerol produced/biomass (mmol g ⁻¹_x )

8.50 ± 0.04

<0.01

<0.01

Ratio acetate consumed/biomass (mmol g ⁻¹_x )

2.44 ± 0.10

6.92 ± 0.12

8.90 ± 0.04

Ratio acetate consumed/glucose (g g⁻¹)

0.011 ± 0.00

0.032 ± 0.00

0.042 ± 0.00