Fig. 6

Putative ATP-driven transhydrogenase cycle for converting cytosolic NADH to NADPH involving Ald6. Acetate can be converted to acetyl-CoA via Acs1/Acs2 at the net cost of 2 ATP (ATP to AMP and pyrophosphate, followed by pyrophosphate hydrolysis). Acetyl-CoA can be converted to acetaldehyde via EutE, using cytosolic NADH as electron donor. Lastly, acetaldehyde is converted back to acetate via Ald6, thereby forming NADPH