ATP citrate lyase mediated cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae
- Sarah Rodriguez†1, 2,
- Charles M. Denby†1, 3,
- T. Van Vu1, 4,
- Edward E. K. Baidoo1, 2,
- George Wang1, 2 and
- Jay D. Keasling1, 2, 3, 4, 5Email author
© Rodriguez et al. 2016
Received: 7 October 2015
Accepted: 23 February 2016
Published: 3 March 2016
With increasing concern about the environmental impact of a petroleum based economy, focus has shifted towards greener production strategies including metabolic engineering of microbes for the conversion of plant-based feedstocks to second generation biofuels and industrial chemicals. Saccharomyces cerevisiae is an attractive host for this purpose as it has been extensively engineered for production of various fuels and chemicals. Many of the target molecules are derived from the central metabolite and molecular building block, acetyl-CoA. To date, it has been difficult to engineer S. cerevisiae to continuously convert sugars present in biomass-based feedstocks to acetyl-CoA derived products due to intrinsic physiological constraints—in respiring cells, the precursor pyruvate is directed away from the endogenous cytosolic acetyl-CoA biosynthesis pathway towards the mitochondria, and in fermenting cells pyruvate is directed towards the byproduct ethanol. In this study we incorporated an alternative mode of acetyl-CoA biosynthesis mediated by ATP citrate lyase (ACL) that may obviate such constraints.
We characterized the activity of several heterologously expressed ACLs in crude cell lysates, and found that ACL from Aspergillus nidulans demonstrated the highest activity. We employed a push/pull strategy to shunt citrate towards ACL by deletion of the mitochondrial NAD+-dependent isocitrate dehydrogenase (IDH1) and engineering higher flux through the upper mevalonate pathway. We demonstrated that combining the two modifications increases accumulation of mevalonate pathway intermediates, and that both modifications are required to substantially increase production. Finally, we incorporated a block strategy by replacing the native ERG12 (mevalonate kinase) promoter with the copper-repressible CTR3 promoter to maximize accumulation of the commercially important molecule mevalonate.
By combining the push/pull/block strategies, we significantly improved mevalonate production. We anticipate that this strategy can be used to improve the efficiency with which industrial strains of S. cerevisiae convert feedstocks to acetyl-CoA derived fuels and chemicals.
KeywordsATP citrate lyase Mevalonate pathway Saccharomyces cerevisiae Acetyl Coenzyme A Metabolic engineering Isoprenoid synthesis
Increasing environmental concerns about the sustainability of producing petroleum-derived chemicals has led to increased interest in alternative means of production. Metabolic engineering of microorganisms to convert biomass and organic waste to chemicals that are ordinarily derived from petroleum represents a more sustainable production strategy. The yeast Saccharomyces cerevisiae is a commonly used microbial cell factory for metabolic engineering [2, 3, 28] as it is tolerant of industrial conditions, is extraordinarily well-characterized, and offers tools for genetic engineering [19, 20, 29]. While an immense amount of progress has been made, there remains room to improve the efficiency with which engineered microbes produce chemicals from feedstocks.
Acetyl-CoA is a key molecule in central carbon metabolism , as it is required for basic cellular functions such as energy metabolism, lipid metabolism, and amino acid metabolism. Acetyl-CoA also serves as a precursor for the biosynthesis of many industrial chemicals including lipids (dietary supplements and biodiesels), polyketides (antibiotics and anticancer drugs), polyhydroxyalkanoates (biodegradable polymers), and isoprenoids (flavors and fragrances, biodiesels, anti-microbials and anti-cancer drugs, rubber, cosmetic additives and vitamins). In addition, the isoprenoid pathway intermediate mevalonate has been cited for its use as a precursor for biobased production of β-methyl-δ-valerolactone, which can be transformed into a rubbery polymer .
In S. cerevisiae, cytoplasmic acetyl-CoA is endogenously produced from pyruvate through three metabolic steps called the pyruvate dehydrogenase (PDH) bypass. However, due to the nature of the endogenous regulatory metabolism, production of acetyl-CoA derived metabolites via the native cytoplasmic PDH bypass is constrained: when grown at high glucose concentrations a significant portion of glucose is converted to reduced byproducts such as ethanol , whereas at low glucose concentrations the bulk of pyruvate is transported to the mitochondria .
Once pyruvate enters the mitochondrion it can be converted to acetyl-CoA by the PDH complex, however, this mitochondrial acetyl-CoA pool is not available to cytoplasmic biosynthesis pathways.
In this study, we reconstituted the salient features of the ACL pathway in S. cerevisiae through genetic engineering and media optimization. As a proof of principle, we demonstrate that this alternative mode of metabolism can be used to significantly increase the yield of the acetyl-CoA derived product mevalonate. E. coli has also been engineered to produce mevalonate as a final product . While the mevalonate pathway in S. cerevisiae has been extensively engineered to produce various isoprenoid products, this is the first study to our knowledge, to produce mevalonate as a final product.
Media, cultivation, and transformation
For strain construction, pre-cultures were grown with 5 mL of Yeast extract + Peptone + Dextrose (YPD) medium in glass test tubes with shaking at 200 rpm. After 18 h of growth, pre-cultures were used to inoculate 50-mL cultures in 250-mL Erlenmeyer flasks. After ~6 h of growth, strains were transformed by the lithium acetate method . For construction of IDH1::HygB, the Hygromycin B resistance cassette was amplified from pUG32  with primers containing 40 base pairs corresponding to the chromosomal sequence immediately 5′ and 3′ of the IDH1 locus. For construction of strains containing integrated heterologous gene sequences, the integration fragments were amplified from their corresponding plasmids (Additional file 1: Table S1). Each integration fragment contained the heterologous genes and either an auxotrophic complementation or drug resistance cassette, with flanking 750 base pairs corresponding to the site of chromosomal integration. To select for DNA transformants containing auxotrophic complementation cassettes, cells were plated on standard dropout medium (Sunrise, San Diego), and for DNA transformants containing drug resistance cassettes, cells were plated on YPD, grown for 24 h, and then replica plated to YPD supplemented with 200 μg/L Geneticin (Sigma, cat. no. A1720) or Hygromycin B (Sigma, cat. no. H7772).
For the ACL activity assay, single colonies were used to inoculate 5 mL Yeast extract + Peptone (YP) + 2 % galactose liquid cultures. Cultures were then diluted into fresh medium to an OD of 0.05, and grown for 24 h.
For metabolomics and production experiments, strains were grown at 30 °C with shaking at 200 rpm in a base medium composed of 6.7 g/L Yeast Nitrogen Base (YNB) without amino acids, without ammonium sulfate (Difco), and 0.7 g/L Complete Supplemented Media (CSM) amino acid mixture (Sunrise, San Diego). Media were supplemented with additional components as specified below. For metabolomics quantification, cultures were grown in the base medium supplemented with 2 % raffinose and 0.1 % dextrose. Five milliliter pre-cultures were inoculated from single colonies and grown for 36 h in test tubes. Production cultures (50 mL) supplemented with 2 % galactose were inoculated to an initial OD600 of 0.05 in 250-mL Erlenmeyer flasks. Low nitrogen cultures were supplemented with 7 mg/L ammonium sulfate, and standard nitrogen cultures were supplemented with 1 g/L ammonium sulfate.
For experiments ‘Increasing pull on cytoplasmic acetyl-CoA towards the mevalonate pathway,’ 10-mL pre-cultures supplemented with 2 % galactose were grown for 36 h in test tubes. These cultures were used to inoculate an additional 10-mL pre-culture to an initial OD600 of 0.2, which was grown for 6 h. The resulting cultures were centrifuged at 3000×g and washed once with fresh base medium. A fraction of the resulting cell suspension was centrifuged and resuspended in 10 mL of fresh base medium in test tubes so as to reach an initial OD600 of 0.5, and then 2 × 7 mm silicone elastomer galactose discs (Kuhner) were added.
Strains and plasmids
MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::hphMX4
BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 trp1::EfMvaE-EfMvaS–SpHIS5
BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::hphMX4 trp1::EfMvaE-EfMvaS–SpHIS5
BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::ylIDH–CaURA3 trp1::EfMvaE-EfMvaS–SpHIS5
BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::hphMX4 ERG12::KlLeu2–PCTR3-ERG12
BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 ERG12::KlLeu2–PCTR3-ERG12
MvaE was codon optimized for S. cerevisiae and synthesized by Genscript (Piscataway, New Jersey). The coding sequences were obtained from NCBI cataloged under accession: AF290092.1 GI: 9937382. MvaS was amplified directly from the Enterococcus faecalis genome, with primers designed to amplify from the nucleotide sequence obtained from the same NCBI accession number.
Expression plasmids used in this study
JBEI registry number
Yeast episomal plasmid with 2μ-origin and LEU2 selectable marker
Stratagene, La Jolla, CA
Yeast episomal plasmid with 2μ-origin and URA3 selectable marker
Stratagene, La Jolla, CA
JBEI-7134 (pACLab). Two multi-copy plasmids, pRS426  and pESC-URA (Stratagene, La Jolla), were previously combined to form plasmid pADS-AMO-CPR . This plasmid contained genes encoding amorphadiene synthase (ADS), amorphadiene oxidase (AMO) and its redox partner cytochrome P450 reductase (CPR). We removed the AMO and CPR genes and replaced them with ACLa and ACLb genes, respectively, to form pESC-P GAL1 ACLa-P GAL10 ACLb-P GAL1-ADS-LEU2d (JBEI-3271) plasmid. Then ADS was removed by yeast homologous recombination. Phosphorylated primers, with 20 overhanging base pairs and matching ends homologous to the promoter or terminator regions surrounding ADS, were designed to amplify the pESC-P GAL1 ACLa-P GAL10 ACLb-P GAL1-ADS-LEU2d vector regions surrounding the ADS gene by PCR. The PCR product was transformed into yeast and colonies were screened for the removal of ADS by PCR. The resulting construct was purified using a modified Miniprep protocol (described above) and transformed into DH10b competent cells for propagation.
JBEI-10632 (pMvaES). This plasmid was constructed from Keasling-2159 by replacing AMO with mvaE and CPR with mvaS using standard yeast homologous recombination. Replacement of the LEU2d marker for the URA3 marker was performed by cutting the plasmid once within the LEU2d marker, treatment of the plasmid with phosphatase, and co-transformation of the treated cut plasmid with PCR product of the URA3 cassette, with flanking ends homologous to the site of integration within the vector.
JBEI-10650. DNA from Y. Lipolytica was prepared using the modified Mini prep protocol described above. The pESC-LEU2d backbone, the Y.l.IDH1 and P GAL1/10 fragments were amplified for Gibson assembly-based construction as described above. The genomic Y.l.IDH2 gene contains an intron 24 base pairs 3′ from the start of the coding sequence. In order to remove the intron, the intron-less coding sequence was broken into two parts. The first part was amplified from a 500-bp gBlock (IDT), while the second part was amplified from genomic DNA.
ACL Activity assay
Cell lysates were prepared as follows: 20 OD units of three biological replicate cultures were centrifuged at 3300×g for 10 min. Cells were washed in 2 mL of 0.1 M Tris buffer, pH 8.7 supplemented with 10 µL protease inhibitor (Sigma cat. no. P8215) and then resuspended in 500 µL of the same buffer. 500 µL of glass beads (USA scientific) were added, and cells were broken using a benchtop homogenizer/bead beating instrument (FastPrep-24, MP Biomedicals). Cells were shaken 5 × 20 s intervals, separated by one-minute intervals on ice. Beads and cellular debris were centrifuged at 10,000×g for 2 min at 4 °C. 300 µL of supernatant were collected as the cell lysate.
ACL activity assays were performed as described previously  with minor modifications. Briefly, 20 µL of cell lysate was added to a mixture composed of 100 mM Tris–HCl (pH 8.4), 20 mM sodium citrate, 5 u/ml malate dehydrogenase (Sigma), 10 mM MgCl2, 10 mM DTT, 0.15 μM NADH, 0.3 mM Coenzyme A, and 5 mM ATP. Reactions were initiated by adding cell lysate and the decrease in absorption at 340 nm was monitored every 15 s using a SPMAX plate reader set at 30 °C. Lysate activities were calculated as the initial rate of the decrease in absorption at 340 nm. Initial rates were calculated as the slope of a general linearized model for the first 10 min of the reaction. For the ACL-containing strains, activities were reported as the absolute initial rate divided by the initial rate of the parent strain, then divided by total mg protein in each sample as measured by Pierce BCA protein assay kit (Life Technologies).
Citric acid cycle metabolite analysis
Metabolite extraction was performed as previously demonstrated by , modified for S. cerevisiae by use of bead-beating for cell lysis of five OD units of culture. Tri-carboxylic acid (TCA) cycle intermediates were analyzed by liquid chromatography and mass spectrometry (LC–MS). Chemical standards were made up to 200 µM, as the stock solution, in methanol–water (50:50, v/v). The separation of the TCA cycle intermediates was conducted on a ZIC-pHILIC column (150 mm length, 4.6-mm internal diameter, and 5-µm particle size; from Merck SeQuant, and distributed via The Nest Group, Inc., MA., USA) using an Agilent Technologies 1200 Series HPLC system (Agilent Technologies, CA, USA). The sample injection volume was 3 µL. The temperature of the sample tray was maintained at 4 °C using an Agilent FC/ALS Thermostat. The column compartment was set to 40 °C. The mobile phase was composed of (A) 10 mM ammonium carbonate and 0.5 % ammonium hydroxide in acetonitrile–water (2:8, v/v) and (B) 10 mM ammonium carbonate and 0.5 % ammonium hydroxide in acetonitrile–water (8:2, v/v). TCA cycle intermediates were eluted isocratically with a mobile phase composition of 33 % mobile phase A and 67 % of mobile phase B. A flow rate of 0.45 mL/min was used. The HPLC system was coupled to an Agilent Technologies 6210 time-of-flight mass spectrometer (LC-TOF MS) by a 1/6 post-column split. Contact between both instrument set-ups was established using a LAN card in order to trigger the MS into operation upon initiation of a run cycle from the MassHunter workstation (Agilent Technologies, CA, USA). Electrospray ionization (ESI) was conducted in the negative ion mode and a capillary voltage of—3500 V was used. MS experiments were carried out in full scan mode, at 0.86 spectra/second for the detection of [M–H]/Z. Prior to LC-TOF MS analysis, the TOF MS was calibrated via an ESI-L-low concentration tuning mix (Agilent Technologies, CA, USA). Data acquisition and processing were performed by the MassHunter software package. The instrument was tuned for a range of 50–1700 m/z—were quantified via eight-point calibration curve ands ranging from 625 nM to 200 µM. The R2 coefficients for the calibration curves were ≥0.99.
CoA metabolite analysis
For CoA metabolite analysis, a method previously established was adapted from . Briefly, 20 OD units of each culture were pelleted (6000 rpm, 5 min, 4 °C). The supernatant was aspirated, and the cells were suspended in 1 mL of 10 % TCA containing crotonyl-CoA (10 μM) as an internal standard. The cells were bead-beaten for a total of 5 min (intervals of 20 s of beating followed by 20 s on ice). The supernatant was collected and neutralized with 2 × volume of 1 M octylamine. Samples were then filtered and the neutralized TCA extract was analyzed via LC–MS using electrospray ionization. The LC conditions used were adapted from .
Extracellular metabolites and organic acids detection
Glucose, galactose, acetate, ethanol and glycerol were separated by HPLC and detected by RID and DAD detectors. One mL of cell culture was transferred and centrifuged at 18,000×g for 5 min. The supernatant was then filtered using a Costar® Spin-X® Centrifuge Tube Filters, 0.22-µm pore and applied to an Agilent 1100 series HPLC equipped with an Agilent 1200 series auto-sampler, an Aminex HPX-87H ion exchange column (Biorad), and an Agilent 1200 series DAD and RID detectors. Metabolites were separated using 4 mM H2SO4 aqueous solution with a flow rate of 0.6 ml/min at 50 °C. Galactose consumption was calculated as the concentration of galactose remaining in prepared medium with cultured strains, subtracted from the concentration of galactose in the control culture of prepared media without cells.
Mevalonate and squalene quantification
A detailed description of mevalonate and squalene quantification was recently described . In brief, mevalonate was derivatized to mevalonolactone by mixing 200 µL cell culture with 50 µL 2 M HCl and vortexing for 15 min. Mevalonolactone was extracted into ethyl acetate by adding 250 µL ethyl acetate containing 10 μg/mL caryophyllene (internal standard) and vortexing for 5 min. Samples were centrifuged at 3000×g for 5 min and 100 µL of the organic phase was removed and transferred to glass GC vials for GCMS analysis. 1 μL of sample was injected (splitless), by using He as the carrier gas onto a CycloSil-B column (Agilent, 30-m length, 0.25-mm inner diameter (i. d.), 0.25-μm film thickness, cat. no. 112–6632) using an Agilent GC system 6890 series GCMS with Agilent mass selective detector 5973 network. The carrier gas was held at a constant flow rate of 1.0 mL/minutes. After each sample injection, the oven temperature was held at 90 °C for 1 min, followed by a ramp of 30 °C/minute to a final temperature of 250 °C, and then held at 250 °C for 2 min. Solvent delay was set to 3.5 min, EMV mode was set to a gain factor of 1, and the MS instrument was set to SIM for acquisition, monitoring m/z ions 58 and 71 (mevalonolactone ions), along with 189 and 204 (caryophyllene internal standard ions). Peak areas for mevalonolactone and caryophyllene were quantified using MSD Productivity ChemStation software (Agilent), and relative mevalonolactone levels were calculated as the quotient of mevalonolactone and caryophyllene. For absolute quantification, sample values were fit to a generalized linear model generated from mevalonolactone standards.
Squalene was extracted by resuspending cells from 2 mL culture in 0.4 mL of the alcoholic KOH solution containing 10 μg/mL cholesterol, and boiling for 5 min. Squalene was extracted from the alcoholic solution by adding 0.4 mL of dodecane and vortexing for 5 min. Samples were centrifuged at 3000×g for 5 min and 100 µL of the organic phase was removed to glass GC vials. GCMS analysis and quantification were as described above with the following modifications: samples were injected onto a DB-5MS column (Agilent); after each sample injection, the oven temperature was held at 80 °C for 1 min, followed by a ramp of 20 °C/min to 280 °C, and then held for 15 min at 280 °C and a ramp to 300 °C at a rate of 20 °C/min with a final hold at 300 °C for 2 min.; solvent delay was set to 10 min, EMV mode was set to a gain factor of 1, the mass spectrometer was set to SIM acquisition mode, monitoring m/z ions 218, 386 and 396, and the temperatures of the quadrupole and the ion source were set to 200 and 300 °C, respectively.
Activity of ACLs from different sources
Engineering increased intracellular flux through ACL
Surprisingly, the total cellular acetyl-CoA concentration decreased in both limited and standard nitrogen conditions with the expression of ACL (Fig. 3c). Because there are myriad cellular pathways that consume acetyl-CoA , we reasoned that total acetyl-CoA levels may not reflect flux through ACL. Consistently, several previous studies have demonstrated that total cellular acetyl-CoA concentrations between strains do not correlate with production of acetyl-CoA derived products [31, 39].
One of the major sinks for cytoplasmic acetyl-CoA in S. cerevisiae is the isoprenoid pathway, which leads to the biosynthesis of ubiquinone, prenylated proteins, and sterols (Fig. 1). Previous work has shown that strains engineered for isoprenoid production exhibit accumulation of the pathway intermediate mevalonate [30, 36]. We observed an increase in mevalonate suggesting that ACL expression may increase flux toward the mevalonate pathway, however the effect was modest (30 %) (Fig. 3c). We reasoned that the limited increase in flux to acetyl-CoA-dependent pathways was likely due to insufficient citrate supply.
In order to increase citrate supply to ACL, we attempted to further mimic the lipid accumulation phase of oleaginous yeast central carbon metabolism, by shunting the citrate destined for the TCA cycle to the cytoplasm. In oleaginous yeast, the major regulatory mechanism controlling the shift of citrate flux from the TCA cycle to ACL is mediated by inhibition of mitochondrial ICDH [9, 10]. S. cerevisiae contains both NADP+- and NAD+-dependent mitochondrial ICDHs. In a wild-type background the NAD+-dependent mitochondrial ICDH mediates the bulk of the cellular flux . It has been shown previously that deletion of IDH1, which encodes the regulatory subunit of NAD+-dependent mitochondrial ICDH, results in increased citrate concentration , suggesting that IDH1Δ could effectively shunt citrate from the TCA cycle to the cytoplasm. This prompted us to measure the effect of IDH1Δ and ACL overexpression on the levels of other key central carbon metabolites.
Changes in metabolite concentrations in strains with IDH1Δ and ACL expression strongly suggest that we successfully reconstituted this key aspect of oleaginous yeast metabolism. First, isocitrate was undetectable in IDH1 + strains, but was clearly detectable in the IDH1Δ background, indicating that flux through ICDH was effectively blocked (Fig. 3b). Second, while citrate and isocitrate concentrations increased in the IDH1Δ background, both decreased dramatically when ACL was expressed, indicating that a substantial fraction of cellular citrate was transported from the mitochondrion to the cytoplasm and consumed by ACL (46 % decrease in citrate concentrations with ACL expression) (Fig. 3b, c). Third, while the mevalonate concentration decreased in the IDH1Δ background, it increased when ACL was expressed (96 % increase) (Fig. 3d), suggesting that shunting citrate from the TCA cycle may allow additional acetyl-CoA produced by ACL to be directed to acetyl-CoA dependent biosynthesis pathways.
Other key metabolites of the TCA cycle and glyoxylate shunt were measured (Additional file 2: Figure S1). IDH1 ∆-modified strains demonstrate fold increases of intracellular concentrations of malate, fumarate and succinate by 2.5, 2.2, and 1.8, respectively, as compared to the wild type strain (Additional file 2: Figure S1). Interestingly, these metabolites are key products of the glyoxylate cycle, and large increases in intracellular concentrations of malate, fumarate, and succinate in IDH1∆ strains may be indicative of utilization of the glyoxylate cycle. Notably, 2-oxoglutarate was also found to significantly increase (205 % increase) with ACL expression, as compared to the IDH∆ background without ACL (Additional file 2: Figure S1). We speculate that the expression of ACL leads to down stream effects which replenish the mitochondria with 2-oxoglutarate for further oxidative phosphorylation.
Increasing pull on cytoplasmic acetyl-CoA towards the mevalonate pathway
We next set out to test whether our strategy could be used to improve biosynthesis of acetyl-CoA derived products in a strain engineered for greater pull towards the mevalonate pathway. In particular we focused on production of the acetyl-CoA derived pathway intermediate mevalonate, because of its potential use as a precursor for biobased production of β-methyl-δ-valerolactone . Acetyl-CoA flux was directed to the mevalonate pathway by integrating two genes from Enterococcus faecalis, mvaE and mvaS, that together encode the first three steps of the mevalonate pathway [17, 43, 44] (Fig. 1). Functional expression of these genes has not previously been demonstrated in S. cerevisiae to our knowledge.
Interestingly, the best producing strain (JBEI 10691–IDH1Δ; ACL; mvaES) also exhibited substantially less growth than the IDH1 + strains (Additional file 3: Figure S2). We reasoned that this may have been caused by reduced citrate flux to the TCA cycle. We initially hypothesized that replacing S. cerevisiae ICDH with one from an oleaginous yeast might achieve a flux distribution between the TCA cycle and the ACL pathway that would allow for improved growth and similar or higher titers, as previous work has shown that ICDH from oleaginous yeast are responsive to the carbon/nitrogen molar ratio . To test this, genes from Y. lipolytica with homology to S. cerevisiae IDH1 and IDH2 were identified, and their functionality was confirmed through complementation of S. cerevisiae IDH1/2Δ (Additional file 4: Figure S3). However, when the Y. lipolytica ICDH replaced IDH1, we observed reduced production compared with IDH1Δ (Fig. 4). The failure of Y.l.IDH1/2 to improve mevalonate production is likely due to the different regulatory environment between oleaginous yeasts and S. cerevisiae.
Engineering a strain for high mevalonate production
In this study the yeast S. cerevisiae was engineered with an alternative mode of acetyl-CoA biosynthesis mediated by ACL in conjunction with a push/pull/block strategy. As a proof of principle, we demonstrate that this alternative mode of metabolism can be used as a strategy to significantly increase the yield of the acetyl-CoA derived product mevalonate. We demonstrate the basic requirements for directing flux through the ACL pathway: increased citrate supply, expression of an active ACL, increased pull towards a product of interest, and limitation of competing downstream pathways. We anticipate that this strategy can be extended to the production of a wide array of acetyl-CoA derived molecules.
SR, CMD, VTV conducted the experiments; SR, CMD, and JDK wrote the manuscript; EEB and GW performed metabolomics analysis. All authors read and approved the final manuscript.
This work was funded by a grant from the National Science Foundation (MCB-1330914) and by the Joint BioEnergy Institute, which is funded by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, under Contract DEAC02-05CH11231. The authors would like to acknowledge the work of Samuel Deutsch and Matthew Hamilton of the Joint Genome Institute for synthesis of ACL constructs.
JDK has a financial interest in Amyris and Lygos. The remaining authors declare that they have no competing interests.
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