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Table 1 Strains and plasmids

From: ATP citrate lyase mediated cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae

Strain

Genotype

Reference

BY4741

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

Euroscarf

JBEI-10683

BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4

This study

JBEI-10684

BY4741 ura3::PGAL1-RtACL-kanMX4

This study

JBEI-10685

BY4741 ura3::PGAL1-MmACL-kanMX4

This study

JBEI-10686

BY4741 ura3::PGAL10-YlACLb-PGAL1-YlACLa-kanMX4

This study

JBEI-10687

BY4741 ura3::PGAL10-LsACLb-PGAL1-LsACLa-kanMX4

This study

JBEI-10688

BY4741 idh1::kanMX4

Euroscarf

JBEI-10689

BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::hphMX4

This study

JBEI-10569

BY4741 trp1::EfMvaE-EfMvaSSpHIS5

This study

JBEI-10690

BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 trp1::EfMvaE-EfMvaSSpHIS5

This study

JBEI-10691

BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::hphMX4 trp1::EfMvaE-EfMvaSSpHIS5

This study

JBEI-10692

BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::ylIDHCaURA3 trp1::EfMvaE-EfMvaSSpHIS5

This study

JBEI-10693

BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 idh1::hphMX4 ERG12::KlLeu2–PCTR3-ERG12

This study

JBEI-10694

BY4741 ura3::PGAL10-AnACLb-PGAL1-AnACLa-kanMX4 ERG12::KlLeu2–PCTR3-ERG12

This study

  1. Yeast strains used in this study