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  1. Olive mill wastewater (OMW) is the aqueous effluent of olive oil producing processes. Given its high COD and content of phenols, it has to be decontaminated before being discharged. Anaerobic digestion is one ...

    Authors: Lorenzo Bertin, Maria Chiara Colao, Maurizio Ruzzi, Leonardo Marchetti and Fabio Fava
    Citation: Microbial Cell Factories 2006 5:16
  2. The quantitative detection of low analyte concentrations in complex samples is becoming an urgent need in biomedical, food and environmental fields. Biosensors, being hybrid devices composed by a biological re...

    Authors: Rosa María Ferraz, Andrea Vera, Anna Arís and Antonio Villaverde
    Citation: Microbial Cell Factories 2006 5:15
  3. Brucella abortus is a facultative intracellular pathogen that mainly infects cattle and humans. Current vaccines rely on live attenuated strains of B. abortus, which can revert to their pathogenic status and thus...

    Authors: Anderson Miyoshi, Luis G Bermúdez-Humarán, Luciana A Ribeiro, Yves Le Loir, Sérgio C Oliveira, Philippe Langella and Vasco Azevedo
    Citation: Microbial Cell Factories 2006 5:14
  4. Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in professional and non-professional phagocytes, and cause abortion in domestic animals and undulant fever in hu...

    Authors: Patrícia Gomes Cardoso, Gilson Costa Macedo, Vasco Azevedo and Sergio Costa Oliveira
    Citation: Microbial Cell Factories 2006 5:13
  5. Due to its unique capability to analyze a large number of single cells for several parameters simultaneously, flow cytometry has changed our understanding of the behavior of cells in culture and of the populat...

    Authors: Diethard Mattanovich and Nicole Borth
    Citation: Microbial Cell Factories 2006 5:12
  6. The biotreatability of actual-site polychlorinated biphenyl (PCB)-contaminated soils is often limited by their poor content of autochthonous pollutant-degrading microorganisms. In such cases, inoculation might...

    Authors: Sara Di Toro, Giulio Zanaroli and Fabio Fava
    Citation: Microbial Cell Factories 2006 5:11
  7. Development of nucleic acid-based detection systems is the main focus of many research groups and high technology companies. The enormous work done in this field is particularly due to the broad versatility an...

    Authors: Magdalena Gabig-Ciminska
    Citation: Microbial Cell Factories 2006 5:9
  8. Shake flasks are widely used because of their low price and simple handling. Many researcher are, however, not aware of the physiological consequences of oxygen limitation and substrate overflow metabolism tha...

    Authors: Antti Vasala, Johanna Panula, Monika Bollók, Lutz Illmann, Christian Hälsig and Peter Neubauer
    Citation: Microbial Cell Factories 2006 5:8
  9. Specific complex interactions between soil bacteria belonging to Rhizobium, Sinorhizobium, Mesorhizobium, Phylorhizobium, Bradyrhizobium and Azorhizobium commonly known as rhizobia, and their host leguminous plan...

    Authors: Anna Skorupska, Monika Janczarek, Małgorzata Marczak, Andrzej Mazur and Jarosław Król
    Citation: Microbial Cell Factories 2006 5:7
  10. Heat and cold shock response are normally considered as independent phenomena. A small amount of evidence suggests instead that interactions may exist between them in two Lactococcus strains.

    Authors: Mario Varcamonti, Slavica Arsenijevic, Luca Martirani, Daniela Fusco, Gino Naclerio and Maurilio De Felice
    Citation: Microbial Cell Factories 2006 5:6
  11. Metabolic pathway manipulation for improving the properties and the productivity of microorganisms is becoming a well established concept. For the production of important metabolites, but also for a better und...

    Authors: Paola Branduardi, Michael Sauer, Luca De Gioia, Giuseppe Zampella, Minoska Valli, Diethard Mattanovich and Danilo Porro
    Citation: Microbial Cell Factories 2006 5:4
  12. Although the citric acid fermentation by Aspergillus niger is one of the most important industrial microbial processes and various aspects of the fermentation appear in a very large number of publications since t...

    Authors: Maria Papagianni and Michael Mattey
    Citation: Microbial Cell Factories 2006 5:3
  13. In an effort to identify alternate recombinant gene expression systems in Pseudomonas fluorescens, we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the an...

    Authors: Diane M Retallack, Tracey C Thomas, Ying Shao, Keith L Haney, Sol M Resnick, Vincent D Lee and Charles H Squires
    Citation: Microbial Cell Factories 2006 5:1
  14. The limited fossil fuel prompts the prospecting of various unconventional energy sources to take over the traditional fossil fuel energy source. In this respect the use of hydrogen gas is an attractive alterna...

    Authors: Debajyoti Dutta, Debojyoti De, Surabhi Chaudhuri and Sanjoy K Bhattacharya
    Citation: Microbial Cell Factories 2005 4:36
  15. Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of...

    Authors: Rigini M Papi, Sotiria A Chaitidou, Fotini A Trikka and Dimitrios A Kyriakidis
    Citation: Microbial Cell Factories 2005 4:35
  16. The solubility of recombinant proteins expressed in bacteria is often disappointingly low. Several strategies have been developed to improve the yield and one of the most common strategies is the fusion of the...

    Authors: Annett Dümmler, Ann-Marie Lawrence and Ario de Marco
    Citation: Microbial Cell Factories 2005 4:34
  17. The composition of cultivation media in relation to strain development for industrial application is reviewed. Heterologous protein production and pentose utilization by Saccharomyces cerevisiae are used to illus...

    Authors: Bärbel Hahn-Hägerdal, Kaisa Karhumaa, Christer U Larsson, Marie Gorwa-Grauslund, Johann Görgens and Willem H van Zyl
    Citation: Microbial Cell Factories 2005 4:31
  18. One of the most fascinating properties of the biotechnologically important organism Saccharomyces cerevisiae is its ability to perform simultaneous respiration and fermentation at high growth rate even under full...

    Authors: Oliver Frick and Christoph Wittmann
    Citation: Microbial Cell Factories 2005 4:30
  19. Native as well as recombinant bacterial cell surface layer (S-layer) protein of Geobacillus (G.) stearothermophilus ATCC 12980 assembles to supramolecular structures with an oblique symmetry. Upon expression in E...

    Authors: Andreas Blecha, Kristof Zarschler, Klaas A Sjollema, Marten Veenhuis and Gerhard Rödel
    Citation: Microbial Cell Factories 2005 4:28
  20. Many enzymes of industrial interest are not in the market since they are bio-produced as bacterial inclusion bodies, believed to be biologically inert aggregates of insoluble protein.

    Authors: Elena García-Fruitós, Nuria González-Montalbán, Montse Morell, Andrea Vera, Rosa María Ferraz, Anna Arís, Salvador Ventura and Antonio Villaverde
    Citation: Microbial Cell Factories 2005 4:27
  21. In 1990 a new approach for vaccination was invented involving injection of plasmid DNA in vivo, which elicits an immune response to the encoded protein. DNA vaccination can overcome most disadvantages of conventi...

    Authors: Jacob Glenting and Stephen Wessels
    Citation: Microbial Cell Factories 2005 4:26
  22. This article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. Biofilm formation is a natural process where microbial cells attach to the s...

    Authors: Nasib Qureshi, Bassam A Annous, Thaddeus C Ezeji, Patrick Karcher and Ian S Maddox
    Citation: Microbial Cell Factories 2005 4:24
  23. Reduced protein stability in vivo is a prerequisite to aggregation. While this is merely a nuisance factor in recombinant protein production, it holds a serious impact for man. This review focuses on specific app...

    Authors: Zoya Ignatova
    Citation: Microbial Cell Factories 2005 4:23
  24. The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets huma...

    Authors: Konrad Büssow, Christoph Scheich, Volker Sievert, Ulrich Harttig, Jörg Schultz, Bernd Simon, Peer Bork, Hans Lehrach and Udo Heinemann
    Citation: Microbial Cell Factories 2005 4:21
  25. It has been shown that proteins fused to ubiquitin undergo greater expression in E. coli and are easier to purify and renaturate than nonhybrid foreign proteins. However, there is no commercial source of large qu...

    Authors: Anna Wojtowicz, Anna Mazurkiewicz-Pisarek, Grazyna Plucienniczak, Diana Mikiewicz-Sygula, Luiza Chojnacka, Natalia Lukasiewicz and Andrzej Plucienniczak
    Citation: Microbial Cell Factories 2005 4:17
  26. The nisin-controlled gene expression system NICE of Lactococcus lactis is one of the most widely used expression systems in Gram-positive bacteria. Despite its widespread use, no optimization of the culture condi...

    Authors: Igor Mierau, Kees Olieman, James Mond and Eddy J Smid
    Citation: Microbial Cell Factories 2005 4:16
  27. The NI sin-C ontrolled gene E xpression system NICE of Lactococcus lactis is one of the most widespread used expression systems of Gram-positive bacteria. It is used in more than 100 laboratories for laboratory-s...

    Authors: Igor Mierau, Peter Leij, Iris van Swam, Barry Blommestein, Esther Floris, James Mond and Eddy J Smid
    Citation: Microbial Cell Factories 2005 4:15
  28. Microorganisms are used in large-scale heap or tank aeration processes for the commercial extraction of a variety of metals from their ores or concentrates. These include copper, cobalt, gold and, in the past,...

    Authors: Douglas E Rawlings
    Citation: Microbial Cell Factories 2005 4:13
  29. Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GF...

    Authors: Wei Wen Su
    Citation: Microbial Cell Factories 2005 4:12
  30. As in other Biotechnological fields, the microbial production of recombinant proteins and other biomolecules can be approached from multiple angles through the help of diverse technologies of increasing comple...

    Authors: Antonio Villaverde
    Citation: Microbial Cell Factories 2005 4:10
  31. Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the ...

    Authors: Jaakko Soini, Christina Falschlehner, Christina Mayer, Daniela Böhm, Stefan Weinel, Johanna Panula, Antti Vasala and Peter Neubauer
    Citation: Microbial Cell Factories 2005 4:9
  32. Actinomycetes (Actinobacteria) are highly attractive as cell factories or bioreactors for applications in industrial, agricultural, environmental, and pharmaceutical fields. Genome sequencing of several species o...

    Authors: Nobutaka Nakashima, Yasuo Mitani and Tomohiro Tamura
    Citation: Microbial Cell Factories 2005 4:7
  33. The permanently impaired protein folding during recombinant protein production resembles the stress encountered at extreme temperatures, under which condition the putative holding chaperones, IbpA/IbpB, play a...

    Authors: Ha LeThanh, Peter Neubauer and Frank Hoffmann
    Citation: Microbial Cell Factories 2005 4:6
  34. Carbon dioxide fixation bioprocess in reactors necessitates recycling of D-ribulose1,5-bisphosphate (RuBP) for continuous operation. A radically new close loop of RuBP regenerating reactor design has been prop...

    Authors: Debojyoti De, Debajyoti Dutta, Moloy Kundu, Sourav Mahato, Marc T Schiavone, Surabhi Chaudhuri, Ashok Giri, Vidya Gupta and Sanjoy K Bhattacharya
    Citation: Microbial Cell Factories 2005 4:5
  35. Hydrocarbon-degrading biofilms in the treatment of contaminated groundwaters have received increasing attention due to the role played in the so-called "biobarriers". These are bioremediation systems in which ...

    Authors: Alessandra Di Lorenzo, Mario Varcamonti, Palma Parascandola, Rodolfo Vignola, Adriano Bernardi, Pasquale Sacceddu, Raffaello Sisto and Elisabetta de Alteriis
    Citation: Microbial Cell Factories 2005 4:4

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