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Production and purification of high molecular weight oligomers of Yersinia pestis F1 capsular antigen released by high cell density culture of recombinant Escherichia coli cells carrying the caf1 operon

Authors: Tzvi Holtzman, Yinon Levy, Dino Marcus, Yehuda Flashner, Emanuelle Mamroud, Sara Cohen and Rephael Fass

Citation: Microbial Cell Factories 2006 5(Suppl 1):P98

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Kinetics of aggregation and structural properties of proteins in inclusion bodies studied by Fourier transform infrared spectroscopy

Authors: Antonino Natalello, Diletta Ami, Pietro Gatti-Lafranconi, Ario de Marco, Marina Lotti and Silvia Maria Doglia

Citation: Microbial Cell Factories 2006 5(Suppl 1):P97

Content type: Poster Presentation Published on: 10 October 2006

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Identifying key signatures of highly productive CHO cells from transcriptome and proteome profiles

Authors: Arleen Sanny, Yee Jiun Kok, Robin Philip, Song Hui Chuah, Sze Wai Ng, Kher Shing Tan, Lee Yih Yean, Kathy Wong, Hu Weishou, Miranda Yap and Peter Morin Nissom

Citation: Microbial Cell Factories 2006 5(Suppl 1):P96

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Purification of a chimeric virus-like particle from a complex culture medium

Authors: Luísa Pedro and Guilherme NM Ferreira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P95

Content type: Poster Presentation Published on: 10 October 2006

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Analysis and characterization of different preparations of recombinant human follicle stimulating hormone (hFSH) and of its subunits

Authors: Maria Teresa CP Ribela, Renan F Loureiro, João E Oliveira, Cristiane M Carvalho, Cibele N Peroni and Paolo Bartolini

Citation: Microbial Cell Factories 2006 5(Suppl 1):P91

Content type: Poster Presentation Published on: 10 October 2006

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Expression of functional recombinant rabies virus glycoprotein in Drosophila melanogaster S2 cells

Authors: Adriana Y Yokomizo, Soraia AC Jorge, Renato M Astray, Mariza AG Santos, Irene Fernandes, Orlando G Ribeiro, Denise SPQ Horton, Aldo Tonso and Carlos A Pereira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P89

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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ColE1 derived RNA I as a key molecule in a novel antibiotic free plasmid addiction system

Authors: Irene Pfaffenzeller, Gerald Striedner, Karl Bayer and Reingard Grabherr

Citation: Microbial Cell Factories 2006 5(Suppl 1):P87

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli

Authors: Luis Vidal, Josep López-Santín, Glòria Caminal and Pau Ferrer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P85

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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The expression of truncated form of CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) from genetically modified plant in Escherichia coli

Authors: Stanislav Stuchlík, Satheesh Natarajan and Ján Turña

Citation: Microbial Cell Factories 2006 5(Suppl 1):P83

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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A simple emergency procedure to be used if biotechnological protein production is endangered by bacteriophage infection of Escherichia coli cultures: effective inhibition of bacteriophage lytic development in infected cultures by removing a carbon source from the medium

Authors: Grzegorz Wegrzyn, Marcin Los and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P81

Content type: Poster Presentation Published on: 10 October 2006

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A regulatory acceptable alternative to E. coli: high yield recombinant protein production using the Lactococcus lactis P170 expression system combined with "Reverse electro enhanced dialysis" (REED) for lactate control

Authors: Søren M Madsen, Astrid Vrang, Lars H Pedersen, Sean A MacDonald, Jens-Ulrik Rype and Arvid Garde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P80

Content type: Poster Presentation Published on: 10 October 2006

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N-terminally acetylated tropomyosin generated in E. coli by coexpression of the S. cerevisiae NatB acetylation complex shows functional properties in vitro

Authors: Robin Maytum and Manfred Konrad

Citation: Microbial Cell Factories 2006 5(Suppl 1):P76

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Design and production of multi-bioactive recombinant elastin-like polymer: Mimicking the extracellular matrix

Authors: Alessandra Girotti, F Javier Arias, Ana M Testera and J Carlos Rodriguez-Cabello

Citation: Microbial Cell Factories 2006 5(Suppl 1):P75

Content type: Poster Presentation Published on: 10 October 2006

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Bacillus megaterium as a recombinant protein production host

Authors: Yang Yang, Marco Malten, Rebekka Biedendieck, Wei Wang, Dieter Jahn and Wolf-Dieter Deckwer

Citation: Microbial Cell Factories 2006 5(Suppl 1):P74

Content type: Poster Presentation Published on: 10 October 2006

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Over-expression and single-step purification of human IFN_α8 and human IFN_α2b reveals the highest antiviral activity of human IFN_α8

Authors: Julio César Sánchez García, Alejandro Miranda Ariza, Alexis Musacchio Lassa, Luis Javier González and Vladimir Besada Perez

Citation: Microbial Cell Factories 2006 5(Suppl 1):P73

Content type: Poster Presentation Published on: 10 October 2006

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Genomic and proteomics approaches for vaccine development in Pasteurella multocida

Authors: Keith Al-Hasani, Victoria McCarl, Stephen Bottomley, Ben Adler and John Boyce

Citation: Microbial Cell Factories 2006 5(Suppl 1):P71

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Disruption of the GAS1 gene of Pichia pastoris confers a supersecretory phenotype for Rhizopus oryzae lipase, but not for human trypsinogen

Authors: Hans Marx, Michael Sauer, David Resina, Marina Vai, Danilo Porro, Francisco Valero, Pau Ferrer and Diethard Mattanovich

Citation: Microbial Cell Factories 2006 5(Suppl 1):P69

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Potentials and limitations of prokaryotic and eukaryotic expression systems for recombinant protein production – a comparative view

Authors: Dethardt Müller, Karl Bayer and Diethard Mattanovich

Citation: Microbial Cell Factories 2006 5(Suppl 1):P61

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Automation for higher throughput in protein expression: visions, facts and fictions

Authors: Marion Mahnke, Jean Marc Schlaeppi, Yann Pouliquen, Louise Barys, Catherine Rolvering, Mario Henke, Rita Schmitz, Sabine Geisse and Frank Kolbinger

Citation: Microbial Cell Factories 2006 5(Suppl 1):P60

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Evaluation of the baculovirus and E.coli-expressed non-structural (NS) proteins of bluetongue virus (BTV) as antigen in an indirect or competition ELISA to differentiate infected from vaccinated animals

Authors: Lissette López, Angel Venteo, Miguel Angel Jiménez-Clavero, Juan Luis Carrasco, María José Cano, Elena Soria, Concepción Gómez-Tejedor, Antonio Sanz, Carmen Vela and Paloma Rueda

Citation: Microbial Cell Factories 2006 5(Suppl 1):P59

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Cell culture efforts to reduce glycation in recombinant humanized antibody

Authors: Inn H Yuk, Hung Huynh, Kimberly Leach, Amy Shen, Boyan Zhang, George Dutina, Patrick McKay, Amy Lim and Brad Snedecor

Citation: Microbial Cell Factories 2006 5(Suppl 1):P57

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Limitations using GFP as a protein expression reporter in Pichia pastoris

Authors: Anna Surribas, David Resina, Pau Ferrer and Francisco Valero

Citation: Microbial Cell Factories 2006 5(Suppl 1):P56

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Application of a genome-scale metabolic model to the inference of nutritional requirements and metabolic bottlenecks during recombinant protein production in Escherichia coli

Authors: Sónia Carneiro, Isabel Rocha and Eugénio Ferreira

Citation: Microbial Cell Factories 2006 5(Suppl 1):P52

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Cost-effective production of labeled recombinant proteins in E. coli using minimal medium

Authors: Aleksei Rozkov, Bert Larsson, Robert Björnestedt, Patrik Strömberg, Fredrik Lindqvist and Fritz Schweikart

Citation: Microbial Cell Factories 2006 5(Suppl 1):P44

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Efficient, antibody-mediated allosteric activation of an immobilized, E. coli beta-galactosidase recombinant biosensor

Authors: Rosa M Ferraz, Anna Arís, Gregorio Álvaro and Antonio Villaverde

Citation: Microbial Cell Factories 2006 5(Suppl 1):P43

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Design of improved membrane protein production experiments in yeast: quantitation of the host response

Authors: Nicklas Bonander, Kristina Hedfalk, Christer Larsson, Petter Mostad, Celia Chang, Lena Gustafsson and Roslyn M Bill

Citation: Microbial Cell Factories 2006 5(Suppl 1):S43

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

Authors: Georg Hinterkörner, Gudrun Brugger, Dethardt Müller, Friedemann Hesse, Renate Kunert, Hermann Katinger and Nicole Borth

Citation: Microbial Cell Factories 2006 5(Suppl 1):P37

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Recombinant protein expression system in cold loving microorganisms

Authors: Rosanna Papa, Valentina Rippa, Giovanni Sannia, Gennaro Marino and Angela Duilio

Citation: Microbial Cell Factories 2006 5(Suppl 1):S37

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Cell engineering of Pseudoalteromonas haloplanktis TAC125: construction of a mutant strain with reduced exo-proteolytic activity

Authors: Ermenegilda Parrilli, Angela Maria Cusano, Maria Giuliani and Maria Luisa Tutino

Citation: Microbial Cell Factories 2006 5(Suppl 1):P36

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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A novel yeast expression system based on a hormone-induced transcriptional cascade

Authors: María José Quintero, Miguel Arévalo-Rodríguez, Ángel Cebolla and Sebastián Chávez

Citation: Microbial Cell Factories 2006 5(Suppl 1):S35

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Impact of high throughput technology on recombinant protein production

Authors: Jo J Jones, Angela M Bridges, Andrew P Fosberry, Jonathan Bullock, Ian Hudson, Richard M Hall, Owen Jenkins and Jeffrey A Cole

Citation: Microbial Cell Factories 2006 5(Suppl 1):S30

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Evaluation of antifoams in the expression of a recombinant FC fusion protein in shake flask cultures of Saccharomyces cerevisiae &Pichia pastoris

Authors: William Holmes, Rodney Smith and Roslyn Bill

Citation: Microbial Cell Factories 2006 5(Suppl 1):P30

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Set up and optimization of a fermentation protocol for the production of a human antibody fragment (Fab') express in E. coli. Pre-pilot and cGMP pilot scale studies

Authors: E Riscaldati, A Ciabini, A Baccante, D Moscatelli, MF Errichetti, A Colagrande, S Cencioni, F Marcocci, V Di Cioccio, L Di Ciccio, M Allegretti, F Martin and G Maurizi

Citation: Microbial Cell Factories 2006 5(Suppl 1):P29

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Monitoring of transcript regulation and protein production related stress responses in Pichia pastoris secreting Fab antibody fragments

Authors: Brigitte Gasser, Michael Maurer, Michael Sauer, Markku Saloheimo, Jari Rautio, Merja Penttila and Diethard Mattanovich

Citation: Microbial Cell Factories 2006 5(Suppl 1):S26

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Human Prolactin (hPRL) and Growth Hormone (hGH) distinct behavior under bacteriophage lambda P_L promoter control

Authors: Carlos RJ Soares, Eric KM Ueda, Tais L Oliveira, Susana R Heller and Paolo Bartolini

Citation: Microbial Cell Factories 2006 5(Suppl 1):P25

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Dynamic optimisation of a recombinant BHK-21 culture based on elementary flux analysis and hybrid parametric/nonparametric modeling

Authors: Ana Teixeira, Carlos Alves, Paula Alves, Manuel Carrondo and Rui Oliveira

Citation: Microbial Cell Factories 2006 5(Suppl 1):S25

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Design of transcriptional fusions of stress sensitive promoters and GFP to monitor the overburden of E.coli hosts during recombinant protein production

Authors: Sabine Nemecek, Karoline Marisch, Renata Juric and Karl Bayer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S24

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Nonclassical inclusion bodies in Escherichia coli

Authors: Špela Peternel, Marjan Bele, Vladka Gaberc-Porekar and Viktor Menart

Citation: Microbial Cell Factories 2006 5(Suppl 1):P23

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Monitoring of stress responses

Authors: Thomas Schweder, Britta Jürgen, Birgit Voigt, Daniel Pioch, Michael Hecker, Stefan Evers and Karl-Heinz Maurer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S23

Content type: Oral Presentation Published on: 10 October 2006

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An optimized method to produce halophilic proteins in Escherichia coli

Authors: Julia Esclapez, Ma José Bonete, Mónica Camacho, Carmen Pire, Juan Ferrer, Vanesa Bautista, Rosa Ma Martínez-Espinosa, Basilio Zafrilla, Francisco Pérez-Pomares and Susana Díaz

Citation: Microbial Cell Factories 2006 5(Suppl 1):S22

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Production, purification and structural analysis of a cation efflux membrane protein from Thermus thermophilus

Authors: Olga Kolaj, Hui Li, Elizabeth Burrowes, Jeremy Moore, Martin Caffrey and J Gerard Wall

Citation: Microbial Cell Factories 2006 5(Suppl 1):S21

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Antisense RNA based control of detrimental factors for recombinant gene expression in Escherichia coli – down-regulation of RNase E

Authors: Christian Kemmer and Peter Neubauer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S18

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Point mutation of serine 179 in the human Prolactin (PRL) affects recombinant protein expression, folding and secretion, abolishes PRL nickel (II)-binding and increases heparin binding capacities

Authors: Eric Ueda, Carlos Soares, Ameae Walker and Paolo Bartolini

Citation: Microbial Cell Factories 2006 5(Suppl 1):P17

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Heterologous overexpression of a halophilic α-amylase

Authors: Vanesa Bautista, Julia Esclapez, Rosa Ma Martínez-Espinosa, Francisco Pérez-Pomares, Mónica Camacho and Ma José Bonete

Citation: Microbial Cell Factories 2006 5(Suppl 1):P13

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production

Authors: Ramon Ramon, Oriol Cos, Pau Ferrer, JoséLuis Montesinos and Francisco Valero

Citation: Microbial Cell Factories 2006 5(Suppl 1):S13

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Investigation of the inclusion body formation process by FTIR spectroscopy

Authors: Gerd Margreiter, Manfred Schwanninger, Christian Obinger and Karl Bayer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S11

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Kinetics of aggregation and structural properties of proteins in inclusion bodies studied by Fourier transform infrared spectroscopy

Authors: Antonino Natalello, Diletta Ami, Pietro Gatti-Lafranconi, Ario de Marco, Marina Lotti and Silvia Maria Doglia

Citation: Microbial Cell Factories 2006 5(Suppl 1):S10

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Cellular toxicity triggered by bacterial inclusion bodies

Authors: Nuria González-Montalbán, Antonio Villaverde and Anna Aris

Citation: Microbial Cell Factories 2006 5(Suppl 1):P9

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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Monitoring the Dynamics of Transcription and Translation Within the Time Course of Recombinant E. coli Cultivations

Authors: Karin Dürrschmid, Wolfgang Schmidt-Heck, Thomas Hrebicek, Helga Reischer, Norbert Auer, Gerd Margreiter, Reinhard Guthke, Andreas Rizzi and Karl Bayer

Citation: Microbial Cell Factories 2006 5(Suppl 1):S6

Content type: Oral Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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A sensor of the Unfolded Protein Response to study the stress induced in Yarrowia lipolytica strains by the production of heterologous proteins

Authors: Catherine Madzak and Jean-Marie Beckerich

Citation: Microbial Cell Factories 2006 5(Suppl 1):P5

Content type: Poster Presentation Published on: 10 October 2006

This article is part of a Supplement: Volume 5 Supplement 1
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