- Poster Presentation
- Open Access
Bacillus megaterium as a recombinant protein production host
© Yang et al; licensee BioMed Central Ltd. 2006
- Published: 10 October 2006
- Shake Flask Cultivation
- Ethylene Terephthalate
- Bacillus Megaterium
- Xylose Isomerase
- High Cell Density Cultivation
The gram positive soil bacterium Bacillus megaterium is well known for its industrial utilization for production of various extracellular enzymes as amylases. Recently production and secretion of recombinant proteins in B. megaterium was also studied [1, 2]. In this contribution a homologous model protein (penicillin G amidase (PGA) from B. megaterium ATCC14945) and a heterologous protein (a hydrolase from Thermobifida fusca DSM43793 (TFH)) were used to further investigate and improve the system. Penicillin amidase is applied in the synthesis of semisynthetic penicillins. TFH is able to degrade specific polyesters such as poly (ethylene terephthalate) (PET) or poly (butylene terephthalate) (PBT), which are hither to regarded as 'non-biodegradable' plastics .
In this new host system both proteins were secreted directly into supernatant and the good productivity was obtained from fermentation.
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