Figure 2
From: Bacillus megaterium as a recombinant protein production host
Proteins from 1.5 ml growth medium form samples taken at indicated time points which showed as the lines at the bottom of the graph, precipitated by ammonium sulfate, analyzed by SDS-PAGE and stained by Coomassie Brilliant Blue. G250. The lines at the top of the graph separate the cultivation medium with different Ca2+ concentration 0 mM, 2.5 mM and 5 mM. Lane M shows Precision Plus Protein Standard (Bio-Rad, Muechen).