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  1. Insufficient availability of molecular chaperones is observed as a major bottleneck for proper protein folding in recombinant protein production. Therefore, co-production of selected sets of cell chaperones al...

    Authors: Mónica Martínez-Alonso, Elena García-Fruitós, Neus Ferrer-Miralles, Ursula Rinas and Antonio Villaverde
    Citation: Microbial Cell Factories 2010 9:64
    Content type: Review Published on:

  2. Overexpression of recombinant proteins usually triggers the induction of heat shock proteins that regulate aggregation and solubility of the overexpressed protein. The two-dimensional gel electrophoresis (2-DE...

    Authors: Chung-Hsien Cheng and Wen-Chien Lee
    Citation: Microbial Cell Factories 2010 9:63
    Content type: Research Published on:

  3. Fumonisin B1 is a cancerogenic mycotoxin produced by Fusarium verticillioides and other fungi. Sphingopyxis sp. MTA144 can degrade fumonisin B1, and a key enzyme in the catabolic pathway is an aminotransferase wh...

    Authors: Doris Hartinger, Stefan Heinl, Heidi Elisabeth Schwartz, Reingard Grabherr, Gerd Schatzmayr, Dietmar Haltrich and Wulf-Dieter Moll
    Citation: Microbial Cell Factories 2010 9:62
    Content type: Research Published on:

  4. Conjugate vaccines in which polysaccharide antigens are covalently linked to carrier proteins belong to the most effective and safest vaccines against bacterial pathogens. State-of-the art production of conjug...

    Authors: Julian Ihssen, Michael Kowarik, Sandro Dilettoso, Cyril Tanner, Michael Wacker and Linda Thöny-Meyer
    Citation: Microbial Cell Factories 2010 9:61
    Content type: Research Published on:

  5. Oxidative stress can severely compromise viability of bifidobacteria. Exposure of Bifidobacterium cells to oxygen causes accumulation of reactive oxygen species, mainly hydrogen peroxide, leading to cell death. I...

    Authors: Valeria Mozzetti, Franck Grattepanche, Déborah Moine, Bernard Berger, Enea Rezzonico, Leo Meile, Fabrizio Arigoni and Christophe Lacroix
    Citation: Microbial Cell Factories 2010 9:60
    Content type: Research Published on:

  6. Many workers have reported halotolerant bacteria from saline conditions capable of protease production. However, antibiotic resistance and heavy metal tolerance pattern of such organisms is not documented very...

    Authors: Sanjay K Singh, Vinayak R Tripathi, Rakesh K Jain, Surendra Vikram and Satyendra K Garg
    Citation: Microbial Cell Factories 2010 9:59
    Content type: Research Published on:

  7. To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzy...

    Authors: David Navarro, Marie Couturier, Gabriela Ghizzi Damasceno da Silva, Jean-Guy Berrin, Xavier Rouau, Marcel Asther and Christophe Bignon
    Citation: Microbial Cell Factories 2010 9:58
    Content type: Technical Notes Published on:

  8. The flavin-dependent enzyme pyranose 2-oxidase (P2Ox) has gained increased attention during the last years because of a number of attractive applications for this enzyme. P2Ox is a unique biocatalyst with high...

    Authors: Clara Salaheddin, Yoshimitsu Takakura, Masako Tsunashima, Barbara Stranzinger, Oliver Spadiut, Montarop Yamabhai, Clemens K Peterbauer and Dietmar Haltrich
    Citation: Microbial Cell Factories 2010 9:57
    Content type: Research Published on:

  9. Recent years have seen a huge growth in the market of industrial yeasts with the need for strains affording better performance or to be used in new applications. Stress tolerance of commercial Saccharomyces cerev...

    Authors: Roberto Pérez-Torrado, Joaquín Panadero, María José Hernández-López, José Antonio Prieto and Francisca Randez-Gil
    Citation: Microbial Cell Factories 2010 9:56
    Content type: Research Published on:

  14. Chemical methods of producing dyes involve extreme temperatures and unsafe toxic compounds. Application of oxidizing enzymes obtained from fungal species, for example laccase, is an alternative to chemical syn...

    Authors: Jolanta Polak and Anna Jarosz-Wilkołazka
    Citation: Microbial Cell Factories 2010 9:51
    Content type: Research Published on:

  15. Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system. However, its physiology and cellular metab...

    Authors: Bevan KS Chung, Suresh Selvarasu, Andrea Camattari, Jimyoung Ryu, Hyeokweon Lee, Jungoh Ahn, Hongweon Lee and Dong-Yup Lee
    Citation: Microbial Cell Factories 2010 9:50
    Content type: Research Published on:

  16. The unfolded protein response (UPR) in eukaryotes upregulates factors that restore ER homeostasis upon protein folding stress and in yeast is activated by a non-conventional splicing of the HAC1 mRNA. The spliced...

    Authors: Mouna Guerfal, Stefan Ryckaert, Pieter P Jacobs, Paul Ameloot, Kathleen Van Craenenbroeck, Riet Derycke and Nico Callewaert
    Citation: Microbial Cell Factories 2010 9:49
    Content type: Research Published on:

  17. We describe a method for specific, quantitative and quick detection of human collagen prolyl 4-hydroxylase (C-P4H), the key enzyme for collagen prolyl-4 hydroxylation, in crude samples based on a sandwich ELIS...

    Authors: Ekaterina Osmekhina, Antje Neubauer, Katharina Klinzing, Johanna Myllyharju and Peter Neubauer
    Citation: Microbial Cell Factories 2010 9:48
    Content type: Technical Notes Published on:

  18. Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceuti...

    Authors: Cecilia Ferndahl, Nicklas Bonander, Christel Logez, Renaud Wagner, Lena Gustafsson, Christer Larsson, Kristina Hedfalk, Richard AJ Darby and Roslyn M Bill
    Citation: Microbial Cell Factories 2010 9:47
    Content type: Research Published on:

  19. Hyaluronic acid is one of the biopolymers most commonly used by the pharmaceutical industry. Thus, there is an increasing number of recent works that deal with the production of microbial hyaluronic acid. Diff...

    Authors: José A Vázquez, María I Montemayor, Javier Fraguas and Miguel A Murado
    Citation: Microbial Cell Factories 2010 9:46
    Content type: Research Published on:

  20. The production of stable and soluble proteins is one of the most important steps prior to structural and functional studies of biological importance. We investigated the parallel production in a medium through...

    Authors: Agnès Groisillier, Cécile Hervé, Alexandra Jeudy, Etienne Rebuffet, Pierre F Pluchon, Yann Chevolot, Didier Flament, Claire Geslin, Isabel M Morgado, Déborah Power, Margherita Branno, Hervé Moreau, Gurvan Michel, Catherine Boyen and Mirjam Czjzek
    Citation: Microbial Cell Factories 2010 9:45
    Content type: Research Published on:

  21. A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for t...

    Authors: Anna Giardina, Rosa Alduina, Elvira Gottardi, Valentina Di Caro, Roderich D Süssmuth and Anna M Puglia
    Citation: Microbial Cell Factories 2010 9:44
    Content type: Research Published on:

  22. Erythrose reductase (ER) catalyzes the final step of erythritol production, which is reducing erythrose to erythritol using NAD(P)H as a cofactor. ER has gained interest because of its importance in the produc...

    Authors: Dae-Hee Lee, Ye-Ji Lee, Yeon-Woo Ryu and Jin-Ho Seo
    Citation: Microbial Cell Factories 2010 9:43
    Content type: Research Published on:

  23. Single-use rocking-motion-type bag bioreactors provide advantages compared to standard stirred tank bioreactors by decreased contamination risks, reduction of cleaning and sterilization time, lower investment ...

    Authors: Julia Glazyrina, Eva-Maria Materne, Thomas Dreher, Dirk Storm, Stefan Junne, Thorsten Adams, Gerhard Greller and Peter Neubauer
    Citation: Microbial Cell Factories 2010 9:42
    Content type: Research Published on:

  24. Bacterial inclusion bodies (IBs) are key intermediates for protein production. Their quality affects the refolding yield and further purification. Recent functional and structural studies have revealed that IB...

    Authors: Britta Jürgen, Antje Breitenstein, Vlada Urlacher, Knut Büttner, Hongying Lin, Michael Hecker, Thomas Schweder and Peter Neubauer
    Citation: Microbial Cell Factories 2010 9:41
    Content type: Research Published on:

  25. In Saccharomyces cerevisiae galactose is initially metabolized through the Leloir pathway after which glucose 6-phosphate enters glycolysis. Galactose is controlled both by glucose repression and by galactose ind...

    Authors: Rosa Garcia Sanchez, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund
    Citation: Microbial Cell Factories 2010 9:40
    Content type: Research Published on:

  26. Aeromonas hydrophila is a serious pathogen and can cause hemorrhagic septicemia in fish. To control this disease, antibiotics and chemicals are widely used which can consequently result in "superbugs" and chemica...

    Authors: Ruidong Chen, Zhigang Zhou, Yanan Cao, Yingguo Bai and Bin Yao
    Citation: Microbial Cell Factories 2010 9:39
    Content type: Research Published on:

  27. Evolvability is an intrinsic feature of all living cells. However, newly emerging, evolved features can be undesirable when genetic circuits, designed and fabricated by rational, synthetic biological approache...

    Authors: Kinga Umenhoffer, Tamás Fehér, Gabriella Balikó, Ferhan Ayaydin, János Pósfai, Frederick R Blattner and György Pósfai
    Citation: Microbial Cell Factories 2010 9:38
    Content type: Research Published on:

  28. Staphylococcal (or micrococcal) nuclease or thermonuclease (SNase or Nuc) is a naturally-secreted nucleic acid degrading enzyme that participates in Staphylococcus aureus spread in the infected host. Purified Nuc...

    Authors: Nicolas Trémillon, Nicolas Issaly, Julien Mozo, Thomas Duvignau, Hervé Ginisty, Eric Devic and Isabelle Poquet
    Citation: Microbial Cell Factories 2010 9:37
    Content type: Research Published on:

  29. Glycerol is the major by-product accounting for up to 5% of the carbon in Saccharomyces cerevisiae ethanolic fermentation. Decreasing glycerol formation may redirect part of the carbon toward ethanol production. ...

    Authors: Julien Pagliardini, Georg Hubmann, Carine Bideaux, Sandrine Alfenore, Elke Nevoigt and Stéphane E Guillouet
    Citation: Microbial Cell Factories 2010 9:36
    Content type: Research Published on:

  30. Bioprocess development of recombinant proteins is time consuming and laborious as many factors influence the accumulation of the product in the soluble and active form. Currently, in most cases the development...

    Authors: Juozas Šiurkus, Johanna Panula-Perälä, Uwe Horn, Mario Kraft, Renata Rimšeliene and Peter Neubauer
    Citation: Microbial Cell Factories 2010 9:35
    Content type: Research Published on:

  31. Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among b...

    Authors: Anna Zanfardino, Odile F Restaino, Eugenio Notomista, Donatella Cimini, Chiara Schiraldi, Mario De Rosa, Maurilio De Felice and Mario Varcamonti
    Citation: Microbial Cell Factories 2010 9:34
    Content type: Research Published on:

  32. Alicyclobacillus sp. A4 is thermoacidophilic and produces many glycoside hydrolases. An extremely acidic β-1,4-glucanase (CelA4) has been isolated from Alicyclobacillus sp. A4 and purified. This glucanase with a ...

    Authors: Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo and Bin Yao
    Citation: Microbial Cell Factories 2010 9:33
    Content type: Research Published on:

  33. The filamentous fungus T. reesei effectively degrades cellulose and is known to produce various cellulolytic enzymes such as β-glucosidase, endoglucanase, and cellobiohydrolase. The expression levels of each cell...

    Authors: Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda and Akihiko Kondo
    Citation: Microbial Cell Factories 2010 9:32
    Content type: Research Published on:

  34. The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increa...

    Authors: Chandrasekhar Gurramkonda, Sulena Polez, Natasa Skoko, Ahmad Adnan, Thomas Gäbel, Dipti Chugh, Sathyamangalam Swaminathan, Navin Khanna, Sergio Tisminetzky and Ursula Rinas
    Citation: Microbial Cell Factories 2010 9:31
    Content type: Research Published on:

  35. The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the ...

    Authors: Sampali Banerjee, Jitendra Kumar, Anjali Apte-Deshpande and Sriram Padmanabhan
    Citation: Microbial Cell Factories 2010 9:30
    Content type: Research Published on:

  36. Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, howe...

    Authors: Hakan Dortay and Bernd Mueller-Roeber
    Citation: Microbial Cell Factories 2010 9:29
    Content type: Research Published on:

  37. PhaR which is a repressor protein for microbial polyhydroxyalkanoates (PHA) biosynthesis, is able to attach to bacterial PHA granules in vivo, was developed as an affinity tag for in vitro protein purification. F...

    Authors: Shuang Zhang, Zhi Hui Wang and Guo Qiang Chen
    Citation: Microbial Cell Factories 2010 9:28
    Content type: Research Published on:

  38. Recombinant gene expression is among the most important techniques used both in molecular and medical research and in industrial settings. Today, two recombinant expression systems are particularly well repres...

    Authors: Hans Peter Sørensen
    Citation: Microbial Cell Factories 2010 9:27
    Content type: Commentary Published on:

  39. The green fluorescent protein has revolutionized many areas of cell biology and biotechnology since it is widely used in determining gene expression and for localization of protein expression. Expression of re...

    Authors: Shardul S Salunkhe, Veena A Raiker, Sachin Rewanwar, Prakash Kotwal, Avijeet Kumar and Sriram Padmanabhan
    Citation: Microbial Cell Factories 2010 9:26
    Content type: Research Published on:

  41. Trigonopsis variabilis D-amino acid oxidase (Tv DAO) is a well characterized enzyme used for cephalosporin C conversion on industrial scale. However, the demands on the enzyme with respect to activity, operationa...

    Authors: Sandra Abad, Jozef Nahalka, Gabriele Bergler, S Alison Arnold, Robert Speight, Ian Fotheringham, Bernd Nidetzky and Anton Glieder
    Citation: Microbial Cell Factories 2010 9:24
    Content type: Research Published on:

  42. The filamentous fungus Aspergillus niger is well-known as a producer of primary metabolites and extracellular proteins. For example, glucoamylase is the most efficiently secreted protein of Aspergillus niger, thu...

    Authors: Xin Lu, Jibin Sun, Manfred Nimtz, Josef Wissing, An-Ping Zeng and Ursula Rinas
    Citation: Microbial Cell Factories 2010 9:23
    Content type: Research Published on:

  43. The overexpression of scFv antibody fragments in the periplasmic space of Escherichia coli frequently results in extensive protein misfolding and loss of cell viability. Although protein folding factors such as S...

    Authors: Dave Siak-Wei Ow, Denis Yong-Xiang Lim, Peter Morin Nissom, Andrea Camattari and Victor Vai-Tak Wong
    Citation: Microbial Cell Factories 2010 9:22
    Content type: Research Published on:

  44. Shikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed to produce SA in Escherichia coli strains derived fro...

    Authors: Adelfo Escalante, Rocío Calderón, Araceli Valdivia, Ramón de Anda, Georgina Hernández, Octavio T Ramírez, Guillermo Gosset and Francisco Bolívar
    Citation: Microbial Cell Factories 2010 9:21
    Content type: Research Published on:

  45. Mannans are one of the key polymers in hemicellulose, a major component of lignocellulose. The Mannan endo-1,4-β-mannosidase or 1,4-β-D-mannanase (EC 3.2.1.78), commonly named β-mannanase, is an enzyme that can c...

    Authors: Chomphunuch Songsiriritthigul, Bancha Buranabanyat, Dietmar Haltrich and Montarop Yamabhai
    Citation: Microbial Cell Factories 2010 9:20
    Content type: Research Published on:

  46. Protein over-production in Escherichia coli often results in formation of inclusion bodies (IBs). Some recent reports have shown that the aggregation into IBs does not necessarily mean that the target protein is ...

    Authors: Ermenegilda Parrilli, Maria Giuliani, Gennaro Marino and Maria Luisa Tutino
    Citation: Microbial Cell Factories 2010 9:19
    Content type: Research Published on:

  47. The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use to produce recombinant proteins in prokariotic ce...

    Authors: Norma A Valdez-Cruz, Luis Caspeta, Néstor O Pérez, Octavio T Ramírez and Mauricio A Trujillo-Roldán
    Citation: Microbial Cell Factories 2010 9:18
    Content type: Review Published on:

  48. In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-through...

    Authors: Eva García-Ruiz, Diana Maté, Antonio Ballesteros, Angel T Martinez and Miguel Alcalde
    Citation: Microbial Cell Factories 2010 9:17
    Content type: Research Published on:

  49. In spite of the substantial metabolic engineering effort previously devoted to the development of Saccharomyces cerevisiae strains capable of fermenting both the hexose and pentose sugars present in lignocellulos...

    Authors: Stefan Krahulec, Barbara Petschacher, Michael Wallner, Karin Longus, Mario Klimacek and Bernd Nidetzky
    Citation: Microbial Cell Factories 2010 9:16
    Content type: Research Published on:

  50. Since about three decades, Saccharomyces cerevisiae can be engineered to efficiently produce proteins and metabolites. Even recognizing that in baker's yeast one determining step for the glucose consumption rate ...

    Authors: Giorgia Rossi, Michael Sauer, Danilo Porro and Paola Branduardi
    Citation: Microbial Cell Factories 2010 9:15
    Content type: Research Published on:
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Microbial Cell Factories

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