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Research
Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting bioma...
Microbial Cell Factories 2017 16:129
Published on: 25 July 2017
Research
Filamentous fungi are commonly used as production hosts for bulk enzymes in biotechnological applications. Their robust and quick growth combined with their ability to secrete large amounts of protein directly...
Microbial Cell Factories 2017 16:128
Published on: 25 July 2017
Research
Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. ...
Microbial Cell Factories 2017 16:130
Published on: 25 July 2017
Research
Lipomyces starkeyi is one of the leading lipid-producing microorganisms reported to date; its genetic transformation was only recently reported. Our aim is to engineer L. starkeyi to s...
Microbial Cell Factories 2017 16:126
Published on: 24 July 2017
Research
Microbial reductive dechlorination of polychlorinated biphenyls (PCBs) plays a major role in detoxifying anoxic contaminated freshwater and marine sediments from PCBs. Known members of the phylum Chloroflexi are ...
Microbial Cell Factories 2017 16:127
Published on: 24 July 2017
Review
Plant natural products (PNPs) are widely used as pharmaceuticals, nutraceuticals, seasonings, pigments, etc., with a huge commercial value on the global market. However, most of these PNPs are still being extr...
Microbial Cell Factories 2017 16:125
Published on: 19 July 2017
Review
Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield ...
Microbial Cell Factories 2017 16:124
Published on: 19 July 2017
Research
Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enz...
Microbial Cell Factories 2017 16:123
Published on: 18 July 2017
Research
The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and p...
Microbial Cell Factories 2017 16:122
Published on: 17 July 2017
Research
Propionic acid is used primarily as a food preservative with smaller applications as a chemical building block for the production of many products including fabrics, cosmetics, drugs, and plastics. Biological ...
Microbial Cell Factories 2017 16:121
Published on: 17 July 2017
Research
Nonomuraea dietziae is a promising microorganism to mediate the region-specific monooxygenation reaction of cyclosporine A (CsA). The main product [(4′-OH)MeLeu]4-CsA possesses high an...
Microbial Cell Factories 2017 16:120
Published on: 14 July 2017
Research
Industrial processes for recombinant protein production challenge production hosts, such as the yeast Pichia pastoris, on multiple levels. During a common P. pastoris fed-batch process, cells experience strong ad...
Microbial Cell Factories 2017 16:117
Published on: 11 July 2017
Research
Erythritol is a natural sweetener that is used in the food industry. It is produced as an osmoprotectant by bacteria and yeast. Due to its chemical properties, it does not change the insulin level in the blood...
Microbial Cell Factories 2017 16:118
Published on: 11 July 2017
Research
Bioconversion of d-galacturonic acid to galactaric (mucic) acid has previously been carried out in small scale (50–1000 mL) cultures, which produce tens of grams of galactaric acid. To obtain larger amounts of bi...
Microbial Cell Factories 2017 16:119
Published on: 11 July 2017
Research
Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector...
Microbial Cell Factories 2017 16:116
Published on: 5 July 2017
Review
The biosynthetic pathways of most alcohols are linked to intracellular redox homeostasis, which is crucial for life. This crucial balance is primarily controlled by the generation of reducing equivalents, as w...
Microbial Cell Factories 2017 16:115
Published on: 24 June 2017
Research
γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding o...
Microbial Cell Factories 2017 16:113
Published on: 21 June 2017
Research
Citramalate, a chemical precursor to the industrially important methacrylic acid (MAA), can be synthesized using Escherichia coli overexpressing citramalate synthase (cimA gene). Deletion of gltA encoding citrate...
Microbial Cell Factories 2017 16:114
Published on: 21 June 2017
Research
Lactofen, a member of the diphenylether herbicides, has high activity and is commonly used to control broadleaf weeds. As a post-emergent herbicide, it is directly released to the environment, and easily cause...
Microbial Cell Factories 2017 16:112
Published on: 19 June 2017
Research
The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in t...
Microbial Cell Factories 2017 16:108
Published on: 15 June 2017
Research
The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light-driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired pheno...
Microbial Cell Factories 2017 16:111
Published on: 15 June 2017
Research
Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of be...
Microbial Cell Factories 2017 16:110
Published on: 15 June 2017
Research
Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vin...
Microbial Cell Factories 2017 16:109
Published on: 15 June 2017
Erratum
Microbial Cell Factories 2017 16:107
Published on: 14 June 2017
The original article was published in Microbial Cell Factories 2015 14:170
Research
21-deoxycortisol (21-DF) is the key intermediate to manufacture pharmaceutical glucocorticoids. Recently, a Japan patent has realized 21-DF production via biotransformation of 17-hydroxyprogesterone (17-OHP) b...
Microbial Cell Factories 2017 16:105
Published on: 13 June 2017
