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  1. Research

    Facile aerobic construction of iron based ferromagnetic nanostructures by a novel microbial nanofactory isolated from tropical freshwater wetlands

    Iron based ferromagnetic nanoparticles (IONP) have found a wide range of application in microelectronics, chemotherapeutic cell targeting, and as contrast enhancers in MRI. As such, the design of well-defined ...

    Patricia Jayshree Jacob, Mas Jaffri Masarudin, Mohd Zobir Hussein and Raha Abdul Rahim

    Microbial Cell Factories 2017 16:175

    Published on: 11 October 2017

  2. Research

    Soluble polymorphic bank vole prion proteins induced by co-expression of quiescin sulfhydryl oxidase in E. coli and their aggregation behaviors

    The infectious prion protein (PrPSc or prion) is derived from its cellular form (PrPC) through a conformational transition in animal and human prion diseases. Studies have shown that the interspecies conversion o...

    Romany Abskharon, Johnny Dang, Ameer Elfarash, Zerui Wang, Pingping Shen, Lewis S. Zou, Sedky Hassan, Fei Wang, Hisashi Fujioka, Jan Steyaert, Mentor Mulaj, Witold K. Surewicz, Joaquín Castilla, Alexandre Wohlkonig and Wen-Quan Zou

    Microbial Cell Factories 2017 16:170

    Published on: 4 October 2017

  3. Research

    Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase

    Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C.

    Liang Tian, Skyler J. Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T. Bouvier, Adam M. Guss, Daniel G. Olson and Lee R. Lynd

    Microbial Cell Factories 2017 16:171

    Published on: 4 October 2017

  4. Research

    Metabolic network model guided engineering ethylmalonyl-CoA pathway to improve ascomycin production in Streptomyces hygroscopicus var. ascomyceticus

    Ascomycin is a 23-membered polyketide macrolide with high immunosuppressant and antifungal activity. As the lower production in bio-fermentation, global metabolic analysis is required to further explore its bi...

    Junhua Wang, Cheng Wang, Kejing Song and Jianping Wen

    Microbial Cell Factories 2017 16:169

    Published on: 3 October 2017

  5. Review

    Methods for genetic transformation of filamentous fungi

    Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a pre...

    Dandan Li, Yu Tang, Jun Lin and Weiwen Cai

    Microbial Cell Factories 2017 16:168

    Published on: 3 October 2017

  6. Review

    Novel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli

    Shikimate is an important intermediate in the aromatic amino acid pathway, which can be used as a promising building block for the synthesis of biological compounds, such as neuraminidase inhibitor Oseltamivir...

    Pengfei Gu, Xiangyu Fan, Quanfeng Liang, Qingsheng Qi and Qiang Li

    Microbial Cell Factories 2017 16:167

    Published on: 29 September 2017

  7. Research

    A novel bifunctional acetyl xylan esterase/arabinofuranosidase from Penicillium chrysogenum P33 enhances enzymatic hydrolysis of lignocellulose

    Xylan, the major constituent of hemicellulose, is composed of β-(1,4)-linked xylopyranosyl units that for the backbone, with side chains formed by other chemical moieties such as arabinose, galactose, mannose,...

    Yi Yang, Ning Zhu, Jinshui Yang, Yujian Lin, Jiawen Liu, Ruonan Wang, Fengqin Wang and Hongli Yuan

    Microbial Cell Factories 2017 16:166

    Published on: 26 September 2017

  8. Research

    Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae

    Kaempferol is a flavonol with broad bioactivity of anti-oxidant, anti-cancer, anti-diabetic, anti-microbial, cardio-protective and anti-asthma. Microbial synthesis of kaempferol is a promising strategy because...

    Lijin Duan, Wentao Ding, Xiaonan Liu, Xiaozhi Cheng, Jing Cai, Erbing Hua and Huifeng Jiang

    Microbial Cell Factories 2017 16:165

    Published on: 26 September 2017

  9. Research

    Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis

    Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications. Bacillus licheniformi...

    Peize Liu, Zhen Chen, Lijie Yang, Qingbiao Li and Ning He

    Microbial Cell Factories 2017 16:163

    Published on: 26 September 2017

  10. Research

    Contribution of single amino acid and codon substitutions to the production and secretion of a lipase by Bacillus subtilis

    Bacillus subtilis produces and secretes proteins in amounts of up to 20 g/l under optimal conditions. However, protein production can be challenging if transcription and cotranslationa...

    Pia Skoczinski, Kristina Volkenborn, Alexander Fulton, Anuseema Bhadauriya, Christina Nutschel, Holger Gohlke, Andreas Knapp and Karl-Erich Jaeger

    Microbial Cell Factories 2017 16:160

    Published on: 25 September 2017

  11. Research

    Polycistronic gene expression in Aspergillus niger

    Genome mining approaches predict dozens of biosynthetic gene clusters in each of the filamentous fungal genomes sequenced so far. However, the majority of these gene clusters still remain cryptic because they ...

    Tabea Schuetze and Vera Meyer

    Microbial Cell Factories 2017 16:162

    Published on: 25 September 2017

  12. Research

    Use of the KlADH3 promoter for the quantitative production of the murine PDE5A isoforms in the yeast Kluyveromyces lactis

    Phosphodiesterases (PDE) are a superfamily of enzymes that hydrolyse cyclic nucleotides (cAMP/cGMP), signal molecules in transduction pathways regulating crucial aspects of cell life. PDEs regulate the intensi...

    Silvia Cardarelli, Mauro Giorgi, Fabio Naro, Francesco Malatesta, Stefano Biagioni and Michele Saliola

    Microbial Cell Factories 2017 16:159

    Published on: 22 September 2017

  13. Research

    Pseudomonas stutzeri as an alternative host for membrane proteins

    Studies on membrane proteins are often hampered by insufficient yields of the protein of interest. Several prokaryotic hosts have been tested for their applicability as production platform but still Escherichia c...

    Manuel Sommer, Hao Xie and Hartmut Michel

    Microbial Cell Factories 2017 16:157

    Published on: 20 September 2017

  14. Research

    Effect of cell permeability and dehydrogenase expression on octane activation by CYP153A6-based whole cell Escherichia coli catalysts

    The regeneration of cofactors and the supply of alkane substrate are key considerations for the biocatalytic activation of hydrocarbons by cytochrome P450s. This study focused on the biotransformation of n-oct...

    Bronwyn E. White, Caryn J. Fenner, Martha S. Smit and Susan T. L. Harrison

    Microbial Cell Factories 2017 16:156

    Published on: 20 September 2017

  15. Research

    Biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65

    Lactobacillus species produce biosurfactants that can contribute to the bacteria’s ability to prevent microbial infections associated with urogenital and gastrointestinal tracts and th...

    I. M. C. Morais, A. L. Cordeiro, G. S. Teixeira, V. S. Domingues, R. M. D. Nardi, A. S. Monteiro, R. J. Alves, E. P. Siqueira and V. L. Santos

    Microbial Cell Factories 2017 16:155

    Published on: 19 September 2017

  16. Research

    Comparative assessment of fermentative capacity of different xylose-consuming yeasts

    Understanding the effects of oxygen levels on yeast xylose metabolism would benefit ethanol production. In this work, xylose fermentative capacity of Scheffersomyces stipitis, Spathaspora passalidarum, Spathaspor...

    Henrique César Teixeira Veras, Nádia Skorupa Parachin and João Ricardo Moreira Almeida

    Microbial Cell Factories 2017 16:153

    Published on: 13 September 2017

  17. Research

    The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore

    Bacillus subtilis spores can be used for presentation of heterologous proteins. Two main approaches have been developed, the recombinant one, requiring modification of bacterial genome...

    Wojciech Potocki, Alessandro Negri, Grażyna Peszyńska-Sularz, Krzysztof Hinc, Michał Obuchowski and Adam Iwanicki

    Microbial Cell Factories 2017 16:151

    Published on: 12 September 2017

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