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  1. Content type: Research

    Bifidobacterium inhabiting the human and animal intestinal tract is known for its health-promoting effect. Tolerance to acid stress is crucial for bifidobacteria to survive and then exert their beneficial effects...

    Authors: Yanxia Wei, Jing Gao, Dianbin Liu, Yang Li and Wenli Liu

    Citation: Microbial Cell Factories 2019 18:156

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  2. Content type: Research

    A microorganism engineered for non-native tasks may suffer stresses it never met before. Therefore, we examined whether a Kluyveromyces marxianus strain engineered with a carotenoid biosynthesis pathway can serve...

    Authors: Hsien-Lin Liu, Jui-Jen Chang, Caroline Thia, Yu-Ju Lin, Shou-Chen Lo, Chieh-Chen Huang and Wen-Hsiung Li

    Citation: Microbial Cell Factories 2019 18:155

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  3. Content type: Research

    Mitochondrial and cytoplasmic malate transporter proteins are responsible for transmembrane transport of malate, thereby linking malate metabolism in various subcellular regions of the cell. These transporters...

    Authors: Lu Wang, Huaiyuan Zhang, Yao Zhang and Yuanda Song

    Citation: Microbial Cell Factories 2019 18:154

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  4. Content type: Research

    Dextransucrases are extracellular enzymes, which catalyze the formation of α-1→6-linked glucose polymers from sucrose. These enzymes are exclusively expressed by lactic acid bacteria, which commonly acidify th...

    Authors: Jonas Schmid, Julia Bechtner, Rudi F. Vogel and Frank Jakob

    Citation: Microbial Cell Factories 2019 18:153

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  5. Content type: Research

    The soil bacterium Pseudomonas putida is a promising platform for the production of industrially valuable natural compounds. In the case of isoprenoids, the availability of biosynthetic precursors is a major limi...

    Authors: Sofía Hernandez-Arranz, Jordi Perez-Gil, Dominic Marshall-Sabey and Manuel Rodriguez-Concepcion

    Citation: Microbial Cell Factories 2019 18:152

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  6. Content type: Research

    Escherichia coli (E. coli) is a bacteria that is widely employed in many industries for the production of high interest bio-products such as recombinant proteins. Nevertheless, the use of E. coli for recombinant ...

    Authors: Gema Lozano Terol, Julia Gallego-Jara, Rosa Alba Sola Martínez, Manuel Cánovas Díaz and Teresa de Diego Puente

    Citation: Microbial Cell Factories 2019 18:151

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  7. Content type: Research

    Fine-tuning the aeration for cultivations when oxygen-limited conditions are demanded (such as the production of vaccines, isobutanol, 2–3 butanediol, acetone, and bioethanol) is still a challenge in the area ...

    Authors: Thiago José Barbosa Mesquita, Cíntia Regina Sargo, José Roberto Fuzer Neto, Sheyla Alexandra Hidalgo Paredes, Roberto de Campos Giordano, Antonio Carlos Luperni Horta and Teresa Cristina Zangirolami

    Citation: Microbial Cell Factories 2019 18:150

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  8. Content type: Research

    Polyol esters of fatty acids (PEFA) are a kind of promising biosurfactants and mainly secreted by Rhodotorula strains. In addition, some strains of Rhodotorula are reliable producers of microbial lipid. Therefore...

    Authors: Mengqi Wang, Weian Mao, Xiaoxiang Wang, Fengyi Li, Jiming Wang, Zhe Chi, Zhenming Chi and Guanglei Liu

    Citation: Microbial Cell Factories 2019 18:149

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  9. Content type: Research

    Colonization of Trichoderma spp. is essential for exerting their beneficial functions on the plant. However, the interactions between Trichoderma spp. and plant roots are still not completely understood. The aim ...

    Authors: Xiaohui Meng, Youzhi Miao, Qiumei Liu, Lei Ma, Kai Guo, Dongyang Liu, Wei Ran and Qirong Shen

    Citation: Microbial Cell Factories 2019 18:148

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  10. Content type: Research

    Poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) [P(3HB-co-3HHx)] is a bacterial polyester with high biodegradability, even in marine environments. Ralstonia eutropha has been engineered for the biosynthesis...

    Authors: Mengxiao Zhang, Shunsuke Kurita, Izumi Orita, Satoshi Nakamura and Toshiaki Fukui

    Citation: Microbial Cell Factories 2019 18:147

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  11. Content type: Research

    Roseoflavin, a promising broad-spectrum antibiotic, is naturally produced by the bacteria Streptomyces davaonensis and Streptomyces cinnabarinus. The key enzymes responsible for roseoflavin biosynthesis and the c...

    Authors: Rodrigo Mora-Lugo, Julian Stegmüller and Matthias Mack

    Citation: Microbial Cell Factories 2019 18:146

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  12. Content type: Research

    Simultaneous co-fermentation of mixed sugars is an important feature to consider in the production of ethanol from lignocellulosic biomass hydrolysates because it enhances the overall ethanol yield and volumet...

    Authors: Marco T. Fernández-Sandoval, Juvencio Galíndez-Mayer, Francisco Bolívar, Guillermo Gosset, Octavio T. Ramírez and Alfredo Martinez

    Citation: Microbial Cell Factories 2019 18:145

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  13. Content type: Research

    Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia. Recently, it has been shown to be a potentiall...

    Authors: Qi Liu, Xiaona Shi, Lili Song, Haifeng Liu, Xiangshan Zhou, Qiyao Wang, Yuanxing Zhang and Menghao Cai

    Citation: Microbial Cell Factories 2019 18:144

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  14. Content type: Research

    In most microbial cultivations d-glucose is the main carbon and energy source. However, quantification of d-glucose especially in small scale is still challenging. Therefore, we developed a FRET-based glucose bio...

    Authors: Julia Otten, Niklas Tenhaef, Roman P. Jansen, Johannes Döbber, Lisa Jungbluth, Stephan Noack, Marco Oldiges, Wolfgang Wiechert and Martina Pohl

    Citation: Microbial Cell Factories 2019 18:143

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  15. Content type: Research

    Hirame novirhabdovirus (HIRRV) can infect a wide range of marine and freshwater fish, causing huge economic losses to aquaculture industry. Vaccine development, especially oral vaccine, has become an effective...

    Authors: Lining Zhao, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing and Wenbin Zhan

    Citation: Microbial Cell Factories 2019 18:142

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  16. Content type: Research

    Bacillus subtilis 916 has been identified as an effective biocontrol agent against Rhizoctonia solani, the causal pathogen of rice sheath blight, under greenhouse and field conditions. HPLC analysis showed that s...

    Authors: Xiaoyu Wang, Zhiyi Chen, Hui Feng, Xi Chen and Lihui Wei

    Citation: Microbial Cell Factories 2019 18:141

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  17. Content type: Research

    S-Formylglutathione is hydrolyzed to glutathione and formate by an S-formylglutathione hydrolase (SFGH) ( This thiol esterase belongs to the esterase family and is also known as esterase D. SFGHs contai...

    Authors: Chang Woo Lee, Wanki Yoo, Sun-Ha Park, Ly Thi Huong Luu Le, Chang-Sook Jeong, Bum Han Ryu, Seung Chul Shin, Han-Woo Kim, Hyun Park, Kyeong Kyu Kim, T. Doohun Kim and Jun Hyuck Lee

    Citation: Microbial Cell Factories 2019 18:140

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  18. Content type: Research

    Acrylic acid (AA) is a widely used commodity chemical derived from non-renewable fossil fuel sources. Alternative microbial-based production methodologies are being developed with the aim of providing “green” ...

    Authors: Sarada S. Raghavan, Sharon Chee, Juntao Li, Jeremie Poschmann, Niranjan Nagarajan, Siau Jia Wei, Chandra S. Verma and Farid J. Ghadessy

    Citation: Microbial Cell Factories 2019 18:139

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  19. Content type: Research

    The development of sustainable technologies for plant cell wall degradation greatly depends on enzymes with hydrolytic activities against carbohydrates. The waste by-products of agricultural cereals are import...

    Authors: Tao Tu, Xiaoli Li, Kun Meng, Yingguo Bai, Yuan Wang, Zhenxing Wang, Bin Yao and Huiying Luo

    Citation: Microbial Cell Factories 2019 18:138

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  20. Content type: Research

    Microbial cell factories are widely used in the production of acidic products such as organic acids and amino acids. However, the metabolic activity of microbial cells and their production efficiency are sever...

    Authors: Zhengming Zhu, Jinhua Yang, Peishan Yang, Zhimeng Wu, Juan Zhang and Guocheng Du

    Citation: Microbial Cell Factories 2019 18:136

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  21. Content type: Research

    Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their su...

    Authors: Qianqian Zhuang and Qingsheng Qi

    Citation: Microbial Cell Factories 2019 18:135

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  22. Content type: Research

    The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow,...

    Authors: José M. Pastor, Nuno Borges, Juan P. Pagán, Sara Castaño-Cerezo, Laszlo N. Csonka, Bradley W. Goodner, Kathryn A. Reynolds, Luís G. Gonçalves, Montserrat Argandoña, Joaquín J. Nieto, Carmen Vargas, Vicente Bernal and Manuel Cánovas

    Citation: Microbial Cell Factories 2019 18:134

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  23. Content type: Research

    Heparosan is the unsulfated precursor of heparin and heparan sulfate and its synthesis is typically the first step in the production of bioengineered heparin. In addition to its utility as the starting materia...

    Authors: Asher Williams, Kamil S. Gedeon, Deepika Vaidyanathan, Yanlei Yu, Cynthia H. Collins, Jonathan S. Dordick, Robert J. Linhardt and Mattheos A. G. Koffas

    Citation: Microbial Cell Factories 2019 18:132

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  24. Content type: Research

    The overexpression and purification of membrane proteins is a bottleneck in biotechnology and structural biology. E. coli remains the host of choice for membrane protein production. To date, most of the efforts h...

    Authors: Kerstin Kanonenberg, Jorge Royes, Alexej Kedrov, Gereon Poschmann, Federica Angius, Audrey Solgadi, Olivia Spitz, Diana Kleinschrodt, Kai Stühler, Bruno Miroux and Lutz Schmitt

    Citation: Microbial Cell Factories 2019 18:131

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  25. Content type: Research

    Acetyl-CoA is an important metabolic intermediate and serves as an acetylation precursor for the biosynthesis of various value-added acetyl-chemicals. Acetyl-CoA can be produced from glucose, acetate, or fatty...

    Authors: Shasha Zhang, Wei Yang, Hao Chen, Bo Liu, Baixue Lin and Yong Tao

    Citation: Microbial Cell Factories 2019 18:130

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  26. Content type: Research

    Vitamin K2 (menaquinone, MK-n) is a lipid-soluble vitamin that functions as a carboxylase co-factor for maturation of proteins involved in many vital physiological processes in humans. Notably, long-chain vita...

    Authors: Yue Liu, Eric O. van Bennekom, Yu Zhang, Tjakko Abee and Eddy J. Smid

    Citation: Microbial Cell Factories 2019 18:129

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  27. Content type: Research

    Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate conversion ...

    Authors: Xian Zhang, Rumeng Han, Teng Bao, Xiaojing Zhao, Xiangfei Li, Manchi Zhu, Taowei Yang, Meijuan Xu, Minglong Shao, Youxi Zhao and Zhiming Rao

    Citation: Microbial Cell Factories 2019 18:128

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  28. Content type: Research

    Our laboratory has constructed a Bacillus licheniformis strain that secretes alkaline protease (AprE) with excellent enzymatic properties. B. licheniformis is generally regarded as safe and has a high industrial ...

    Authors: Cuixia Zhou, Huiying Zhou, Huitu Zhang and Fuping Lu

    Citation: Microbial Cell Factories 2019 18:127

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  29. Content type: Research

    Bacterial secretory proteins often require the formation of disulphide bonds outside the cell to acquire an active conformation. Thiol-disulphide oxidoreductases are enzymes that catalyse the formation of disu...

    Authors: Sonia Gullón, Silvia Marín and Rafael P. Mellado

    Citation: Microbial Cell Factories 2019 18:126

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  30. Content type: Research

    Threonine is an essential amino acid that is extensively used in livestock industry as feed supplement due to its pronounced effect in improving the growth performance of animals. Application of genetically en...

    Authors: Ye Heng Lim, Hooi Ling Foo, Teck Chwen Loh, Rosfarizan Mohamad, Raha Abdul Rahim and Zulkifli Idrus

    Citation: Microbial Cell Factories 2019 18:125

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  31. Content type: Research

    The microbial production of useful fuels and chemicals has been widely studied. In several cases, glucose is used as the raw material, and almost all microbes adopt the Embden–Meyerhof (EM) pathway to degrade ...

    Authors: Shuhei Noda, Yutaro Mori, Sachiko Oyama, Akihiko Kondo, Michihiro Araki and Tomokazu Shirai

    Citation: Microbial Cell Factories 2019 18:124

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  32. Content type: Research

    Myxococcus xanthus DK1622 is a model system for studying multicellular development, predation, cellular differentiation, and evolution. Furthermore, it is a rich source of novel secondary metabolites and is widel...

    Authors: Ying-jie Yang, Raghvendra Pratap Singh, Xin Lan, Cheng-sheng Zhang, Duo-hong Sheng and Yi-qiang Li

    Citation: Microbial Cell Factories 2019 18:123

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  33. Content type: Research

    Acetyl xylan esterase plays an important role in the complete enzymatic hydrolysis of lignocellulosic materials. It hydrolyzes the ester linkages of acetic acid in xylan and supports and enhances the activity ...

    Authors: Sachithra Amarin Hettiarachchi, Young-Kyung Kwon, Youngdeuk Lee, Eunyoung Jo, Tae-Yang Eom, Yoon-Hyeok Kang, Do-Hyung Kang, Mahanama De Zoysa, Svini Dileepa Marasinghe and Chulhong Oh

    Citation: Microbial Cell Factories 2019 18:122

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  34. Content type: Research

    Fungal perylenequinonoid (PQ) pigments from Shiraia fruiting body have been well known as excellent photosensitizers for medical and agricultural uses. The fruiting bodies are colonized by a diverse bacterial com...

    Authors: Yan Jun Ma, Li Ping Zheng and Jian Wen Wang

    Citation: Microbial Cell Factories 2019 18:121

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  35. Content type: Research

    Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing a...

    Authors: Wen Wang, Ping He, Dongdong Zhao, Lijun Ye, Longhai Dai, Xueli Zhang, Yuanxia Sun, Jing Zheng and Changhao Bi

    Citation: Microbial Cell Factories 2019 18:120

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  36. Content type: Research

    The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the va...

    Authors: Lukas Marsalek, Verena Puxbaum, Markus Buchetics, Diethard Mattanovich and Brigitte Gasser

    Citation: Microbial Cell Factories 2019 18:119

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  37. Content type: Research

    Enzymatic glycan synthesis has leapt forward in recent years and a number of glucuronosyltransferase (EC have been identified and prepared, which provides a guide to an efficient approach to prepare ...

    Authors: Dan-Hua Meng, Ran-Ran Du, Lu-Zhou Chen, Meng-Ting Li, Fei Liu, Jin Hou, Yi-Kang Shi, Feng-Shan Wang and Ju-Zheng Sheng

    Citation: Microbial Cell Factories 2019 18:118

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  38. Content type: Research

    Rhodosporidium toruloides is a promising host for the production of bioproducts from lignocellulosic biomass. A key prerequisite for efficient pathway engineering is the availability of robust genetic tools and r...

    Authors: Luísa Czamanski Nora, Maren Wehrs, Joonhoon Kim, Jan-Fang Cheng, Angela Tarver, Blake A. Simmons, Jon Magnuson, Miranda Harmon-Smith, Rafael Silva-Rocha, John M. Gladden, Aindrila Mukhopadhyay, Jeffrey M. Skerker and James Kirby

    Citation: Microbial Cell Factories 2019 18:117

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  39. Content type: Research

    Sucrose is an attractive industrial carbon source due to its abundance and the fact that it can be cheaply generated from sources such as sugarcane. However, only a few characterized Escherichia coli strains are ...

    Authors: Elsayed T. Mohamed, Hemanshu Mundhada, Jenny Landberg, Isaac Cann, Roderick I. Mackie, Alex Toftgaard Nielsen, Markus J. Herrgård and Adam M. Feist

    Citation: Microbial Cell Factories 2019 18:116

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  40. Content type: Research

    Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate syn...

    Authors: Yu Fei, Na Li, De-Huai Zhang and Jun-Wei Xu

    Citation: Microbial Cell Factories 2019 18:115

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  41. Content type: Research

    Actinoplanes sp. SE50/110 is a natural producer of acarbose. It has been extensively studied in the last decades, which has led to the comprehensive analysis of the whole genome, transcriptome and proteome. First...

    Authors: Lena Schaffert, Camilla März, Lisa Burkhardt, Julian Droste, David Brandt, Tobias Busche, Winfried Rosen, Susanne Schneiker-Bekel, Marcus Persicke, Alfred Pühler and Jörn Kalinowski

    Citation: Microbial Cell Factories 2019 18:114

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  42. Content type: Research

    Microalgae have been proposed as potential platform to produce lipid-derived products, such as biofuels. Knowledge on the intracellular carbon flow distribution may identify key metabolic processes during lipi...

    Authors: Xiaojie Ren, Jean-Sébastien Deschênes, Réjean Tremblay, Sabine Peres and Mario Jolicoeur

    Citation: Microbial Cell Factories 2019 18:113

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  43. Content type: Research

    Yaks living in the high-altitude hypoxic environment of Tibetan plateau (3600 m) have special gut microbes. However, it is still little research on yak probiotics until now. Therefore, the purpose of our study...

    Authors: Aoyun Li, Yaping Wang, Zhixing Li, Hammad Qamar, Khalid Mehmood, Lihong Zhang, Juanjuan Liu, Hui Zhang and Jiakui Li

    Citation: Microbial Cell Factories 2019 18:112

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  44. Content type: Research

    Bacillus subtilis is developed to be an attractive expression host to produce both secreted and cytoplasmic proteins owing to its prominent biological characteristics. Chromosomal integration is a stable expressi...

    Authors: Chaoyang Zhou, Bin Ye, Shan Cheng, Leizhen Zhao, Yuanxin Liu, Jiandong Jiang and Xin Yan

    Citation: Microbial Cell Factories 2019 18:111

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  45. Content type: Research

    Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopo...

    Authors: Hui Jin, Yunju Jeong, Sang-Ho Yoo, Tony V. Johnston, Seockmo Ku and Geun Eog Ji

    Citation: Microbial Cell Factories 2019 18:110

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  46. Content type: Research

    Metabolic models are indispensable in guiding cellular engineering and in advancing our understanding of systems biology. As not all enzymatic activities are fully known and/or annotated, metabolic models rema...

    Authors: Sara A. Amin, Elizabeth Chavez, Vladimir Porokhin, Nikhil U. Nair and Soha Hassoun

    Citation: Microbial Cell Factories 2019 18:109

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  47. Content type: Research

    In lactic acid bacteria (LAB), acid stress leads to decreases of cell vitality and fermentation yield. Glutamate decarboxylase (GAD) system is regarded as one of the essential acid-resistance mechanisms in LAB...

    Authors: Luchan Gong, Cong Ren and Yan Xu

    Citation: Microbial Cell Factories 2019 18:108

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  48. Content type: Research

    Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due ...

    Authors: Junshu Wang, Fapeng Liu, Tianyuan Su, Yizhao Chang, Qi Guo, Qian Wang, Quanfeng Liang and Qingsheng Qi

    Citation: Microbial Cell Factories 2019 18:107

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