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  1. Lignocellulosic biomass provides a great starting point for the production of energy, chemicals, and fuels. The major component of lignocellulosic biomass is cellulose, the employment of highly effective enzym...

    Authors: Doaa A. Goda, Huda M. Shakam, Mai E. Metwally, Hager A. Abdelrasoul and Mohamed M. Yacout
    Citation: Microbial Cell Factories 2024 23:73
  2. Bacterial surface glycans are assembled by glycosyltransferases (GTs) that transfer sugar monomers to long-chained lipid carriers. Most bacteria employ the 55-carbon chain undecaprenyl phosphate (Und-P) to sca...

    Authors: Emily J. Kay, Manoj K. Dooda, Joseph C. Bryant, Amanda J. Reid, Brendan W. Wren, Jerry M. Troutman and Matthew A. Jorgenson
    Citation: Microbial Cell Factories 2024 23:72
  3. The improvement of yeast tolerance to acetic, butyric, and octanoic acids is an important step for the implementation of economically and technologically sustainable bioprocesses for the bioconversion of renew...

    Authors: Marta N. Mota, Madalena Matos, Nada Bahri and Isabel Sá-Correia
    Citation: Microbial Cell Factories 2024 23:71
  4. We report the successful fabrication of a pharmaceutical cellular bank (PCB) containing magnetotactic bacteria (MTB), which belong to the Magnetospirillum gryphiswaldense MSR1 species. To produce such PCB, we amp...

    Authors: Théo Chades, Raphaël Le Fèvre, Imène Chebbi, Karine Blondeau, François Guyot and Edouard Alphandéry
    Citation: Microbial Cell Factories 2024 23:70
  5. We are interested in converting second generation feedstocks into styrene, a valuable chemical compound, using the solvent-tolerant Pseudomonas putida DOT-T1E as a chassis. Styrene biosynthesis takes place from L...

    Authors: Ana García-Franco, Patricia Godoy, Estrella Duque and Juan L. Ramos
    Citation: Microbial Cell Factories 2024 23:69
  6. Owing to the growing need for natural materials in different fields, studying melanin production from biological sources is imperative. In the current study, the extracellular melanin pigment was produced by t...

    Authors: Amira S. Abd-EL-Aziz, Nermine N. Abed, Amira Y. Mahfouz and Rasha Mohammad Fathy
    Citation: Microbial Cell Factories 2024 23:68
  7. In recent years, the production of inclusion bodies that retain substantial catalytic activity was demonstrated. These catalytically active inclusion bodies (CatIBs) are formed by genetic fusion of an aggregat...

    Authors: Laura Marie Helleckes, Kira Küsters, Christian Wagner, Rebecca Hamel, Ronja Saborowski, Jan Marienhagen, Wolfgang Wiechert and Marco Oldiges
    Citation: Microbial Cell Factories 2024 23:67
  8. Komagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and phosphoenolpyruvate carboxykinase (PEPCK) is induced by glutamate. Glutama...

    Authors: Neetu Rajak, Trishna Dey, Yash Sharma, Vedanth Bellad and Pundi N. Rangarajan
    Citation: Microbial Cell Factories 2024 23:66
  9. Flavokawain B is one of the naturally occurring chalcones in the kava plant (Piper methysticum). It exhibits anticancer, anti-inflammatory and antimalarial properties. Due to its therapeutic potential, flavokawai...

    Authors: Paweł Chlipała, Tomasz Tronina, Monika Dymarska, Monika Urbaniak, Ewa Kozłowska, Łukasz Stępień, Edyta Kostrzewa-Susłow and Tomasz Janeczko
    Citation: Microbial Cell Factories 2024 23:65
  10. Phosphate solubilizing fungi Penicillium oxalicum (POX) and Red yeast Rhodotorula mucilaginosa (Rho) have been applied in Pb remediation with the combination of fluorapatite (FAp), respectively. The secretion of ...

    Authors: Qiang Guan, Xiaohui Cheng, Yue He, Yifan Yan, Lei Zhang, Zhan Wang, Liangliang Zhang and Da Tian
    Citation: Microbial Cell Factories 2024 23:64
  11. Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-fre...

    Authors: Valériane Malika Keita, Yi Qing Lee, Meiyappan Lakshmanan, Dave Siak-Wei Ow, Paul Staniland, Jessica Staniland, Ian Savill, Kang Lan Tee, Tuck Seng Wong and Dong-Yup Lee
    Citation: Microbial Cell Factories 2024 23:63
  12. 1,2-propanediol (1,2-PDO) is widely used in the cosmetic, food, and drug industries with a worldwide consumption of over 1.5 million metric tons per year. Although efforts have been made to engineer microbial ...

    Authors: Daniel Siebert, Erich Glawischnig, Marie-Theres Wirth, Mieke Vannahme, Álvaro Salazar-Quirós, Annette Weiske, Ezgi Saydam, Dominik Möggenried, Volker F. Wendisch and Bastian Blombach
    Citation: Microbial Cell Factories 2024 23:62
  13. Helicobacter pylori (H. pylori) causes chronic gastric disease. An efficient oral vaccine would be mucosa-targeted and offer defense against colonization of invasive infection in the digestive system. Proteolytic...

    Authors: Furui Zhang, Linhan Ni, Zhen Zhang, Xuegang Luo, Xuequan Wang, Wenmiao Zhou, Jiale Chen, Jing Liu, Yuliang Qu, Kunmei Liu and Le Guo
    Citation: Microbial Cell Factories 2024 23:61
  14. The marine black yeasts are characterized by the production of many novel protective substances. These compounds increase their physiological adaptation to multi-extreme environmental stress. Hence, the exopol...

    Authors: Eman H. Zaghloul, Hala H. Abdel-Latif, Asmaa Elsayis and Sahar W.M. Hassan
    Citation: Microbial Cell Factories 2024 23:60
  15. Polyhydroxybutyrate is a biopolymer produced by bacteria and archaea under nitrogen-limiting conditions. PHB is an essential polymer in the bioplastic sector because of its biodegradability, eco-friendliness, ...

    Authors: Teja Mandragutti, Tura Safawo Jarso, Sudhakar Godi, S Sharmila Begum and Beulah K
    Citation: Microbial Cell Factories 2024 23:59
  16. Acetoin, a versatile platform chemical and popular food additive, poses a challenge to the biosafety strain Bacillus subtilis when produced in high concentrations due to its intrinsic toxicity. Incorporating the ...

    Authors: Tao Li, Haixiang Li, Lei Zhong, Yufei Qin, Gege Guo, Zhaoxing Liu, Ning Hao and Pingkai Ouyang
    Citation: Microbial Cell Factories 2024 23:58
  17. Polyhydroxybutyrate (PHB) has emerged as a promising eco-friendly alternative to traditional petrochemical-based plastics. In the present study, we isolated and characterized a new strain of Salinicola salarius, ...

    Authors: Shymaa A. Abdelrahman, Olfat S. Barakat and Marwa N. Ahmed
    Citation: Microbial Cell Factories 2024 23:56
  18. Pichia pastoris is a widely utilized host for heterologous protein expression and biotransformation. Despite the numerous strategies developed to optimize the chassis host GS115, the potential impact of changes i...

    Authors: Bingjie Cheng, Keyang Yu, Xing Weng, Zhaojun Liu, Xuewu Huang, Yuhong Jiang, Shuai Zhang, Shuyan Wu, Xiaoyuan Wang and Xiaoqing Hu
    Citation: Microbial Cell Factories 2024 23:55
  19. Bio-upcycling of plastics is an emerging alternative process that focuses on extracting value from a wide range of plastic waste streams. Such streams are typically too contaminated to be effectively processed...

    Authors: Yannic S. Ackermann, Jan de Witt, Mariela P. Mezzina, Christoph Schroth, Tino Polen, Pablo I. Nikel, Benedikt Wynands and Nick Wierckx
    Citation: Microbial Cell Factories 2024 23:54
  20. To contribute to the discovery of new microbial strains with metabolic and physiological robustness and develop them into successful chasses, Paracoccus pantotrophus DSM 2944, a Gram-negative bacterium from the p...

    Authors: Upasana Pal, Denise Bachmann, Chiara Pelzer, Julia Christiansen, Lars M. Blank and Till Tiso
    Citation: Microbial Cell Factories 2024 23:53
  21. Among the polyhydroxyalkanoate (PHA), poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)] is reported to closely resemble polypropylene and low-density polyethylene. Studies have shown that PHA...

    Authors: Soon Zher Neoh, Hua Tiang Tan, Chanaporn Trakunjae, Min Fey Chek, Pilanee Vaithanomsat, Toshio Hakoshima and Kumar Sudesh
    Citation: Microbial Cell Factories 2024 23:52
  22. In hematologic cancers, including leukemia, cells depend on amino acids for rapid growth. Anti-metabolites that prevent their synthesis or promote their degradation are considered potential cancer treatment ag...

    Authors: Mariana do Nascimento Costa, Thiago Aparecido Silva, Dimitrius Santiago Passos Simões Fróes Guimarães, Rafael Ricci-Azevedo, Felipe Roberti Teixeira, Leonardo Reis Silveira, Marcelo Damário Gomes, Vítor Marcel Faça, Eduardo Brandt de Oliveira, Rodrigo T. Calado and Roberto N. Silva
    Citation: Microbial Cell Factories 2024 23:51
  23. Microbial communities harbor important biotechnological potential in diverse domains, however, the engineering and propagation of such communities still face both knowledge and know-how gaps. More specifically...

    Authors: Alexis Dijamentiuk, Cécile Mangavel, Chloé Gapp, Annelore Elfassy, Anne-Marie Revol-Junelles and Frédéric Borges
    Citation: Microbial Cell Factories 2024 23:50
  24. Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-...

    Authors: Ping Li, Mengjiao Wang, Haiyan Di, Qihang Du, Yipeng Zhang, Xiaoxu Tan, Ping Xu, Chao Gao, Tianyi Jiang, Chuanjuan Lü and Cuiqing Ma
    Citation: Microbial Cell Factories 2024 23:49
  25. The three-finger proteins are a collection of disulfide bond rich proteins of great biomedical interests. Scalable recombinant expression and purification of bioactive three-finger proteins is quite difficult.

    Authors: Jiang Xu, Xiao Lei, Ao Li, Jun Li, Shuxing Li and Lin Chen
    Citation: Microbial Cell Factories 2024 23:48
  26. Peste des petits ruminants (PPR) is an acute, contact infectious disease caused by the small ruminant morbillivirus (SRMV), and its morbidity in goats and sheep can be up to 100% with significant mortality. Na...

    Authors: Miao Sun, Changjiang Wang, Huaye Luo, Yanfei Chen, Guanggang Qu, Jian Chen, Ling Li, Min Zhang and Qinghong Xue
    Citation: Microbial Cell Factories 2024 23:45
  27. Microorganisms must respond to changes in their environment. Analysing the robustness of functions (i.e. performance stability) to such dynamic perturbations is of great interest in both laboratory and industr...

    Authors: Luisa Blöbaum, Luca Torello Pianale, Lisbeth Olsson and Alexander Grünberger
    Citation: Microbial Cell Factories 2024 23:44
  28. Specific productivity (qP) in yeast correlates with growth, typically peaking at intermediate or maximum specific growth rates (μ). Understanding the factors limiting productivity at extremely low μ might reveal ...

    Authors: Corinna Rebnegger, Benjamin L. Coltman, Viktoria Kowarz, David A. Peña, Axel Mentler, Christina Troyer, Stephan Hann, Harald Schöny, Gunda Koellensperger, Diethard Mattanovich and Brigitte Gasser
    Citation: Microbial Cell Factories 2024 23:43
  29. The Lactobacillaceae family comprises many species of great importance for the food and healthcare industries, with numerous strains identified as beneficial for humans and used as probiotics. Hence, there is a g...

    Authors: Marc Blanch-Asensio, Varun Sai Tadimarri, Alina Wilk and Shrikrishnan Sankaran
    Citation: Microbial Cell Factories 2024 23:42
  30. Developing effective vaccines against SARS-CoV-2 that consider manufacturing limitations, equitable access, and acceptance is necessary for developing platforms to produce antigens that can be efficiently pres...

    Authors: Norma A. Valdez‑Cruz, Diego Rosiles-Becerril, Constanza E. Martínez-Olivares, Enrique García‑Hernández, Laura Cobos-Marín, Daniel Garzón, Francisco E. López-Salas, Guadalupe Zavala, Axel Luviano, Alejandro Olvera, Alejandro Alagón, Octavio T. Ramírez and Mauricio A. Trujillo‑Roldán
    Citation: Microbial Cell Factories 2024 23:41
  31. In recent years, biosafety and green food safety standards have increased the demand for immune enhancers and adjuvants. In the present study, recombinant food-grade Lactococcus lactis (r-L. lactis-Tα1-IFN) expre...

    Authors: Zengqi Liu, Suhua Zhang, Hongjiao Hu, He Wang, Yu Qiu, Mingqi Dong, Muping Wang, Ziyang Cui, Hongyu Cui, Yunfeng Wang and Gaoming He
    Citation: Microbial Cell Factories 2024 23:40
  32. Mucosal vaccines have the potential to induce protective immune responses at the sites of infection. Applying CRISPR/Cas9 editing, we aimed to develop a probiotic-based vaccine candidate expressing the HIV-1 e...

    Authors: Nathaniel Ninyio, Katharina Schmitt, Gladys Sergon, Charlotta Nilsson, Sören Andersson and Nikolai Scherbak
    Citation: Microbial Cell Factories 2024 23:39
  33. The biosynthesis of human milk oligosaccharides (HMOs) using several microbial systems has garnered considerable interest for their value in pharmaceutics and food industries. 2′-Fucosyllactose (2′-FL), the mo...

    Authors: Ran You, Lei Wang, Meirong Hu and Yong Tao
    Citation: Microbial Cell Factories 2024 23:38
  34. Overproduction of desired native or nonnative biochemical(s) in (micro)organisms can be achieved through metabolic engineering. Appropriate rewiring of cell metabolism is performed by making rational changes s...

    Authors: Leila Hassani, Mohammad R. Moosavi, Payam Setoodeh and Habil Zare
    Citation: Microbial Cell Factories 2024 23:37
  35. The yeast Saccharomyces cerevisiae is widely used as a host cell for recombinant protein production due to its fast growth, cost-effective culturing, and ability to secrete large and complex proteins. However, on...

    Authors: Garrett Strawn, Ryan W K Wong, Barry P Young, Michael Davey, Corey Nislow, Elizabeth Conibear, Christopher J R Loewen and Thibault Mayor
    Citation: Microbial Cell Factories 2024 23:36
  36. Squalene epoxidase is one of the rate-limiting enzymes in the biosynthetic pathway of membrane sterols and triterpenoids. The enzyme catalyzes the formation of oxidized squalene, which is a common precursor of...

    Authors: Xiao-liu Liu, Jing Xie, Zhen-ni Xie, Can Zhong, Hao Liu, Shui-han Zhang and Jian Jin
    Citation: Microbial Cell Factories 2024 23:34
  37. Growing evidence has demonstrated that cold and humid environmental stress triggers gastrointestinal (GI) disorders. In this study, we explored the effects of intestinal microbiota homeostasis on the intestina...

    Authors: Chen-Yang Zhang, Xin-Xin Peng, Yi Wu, Mai-Jiao Peng, Tiao-Hao Liu and Zhou-Jin Tan
    Citation: Microbial Cell Factories 2024 23:33
  38. Chitinases are widely distributed enzymes that perform the biotransformation of chitin, one of the most abundant polysaccharides on the biosphere, into useful value-added chitooligosaccharides (COS) with a wid...

    Authors: Marina Minguet-Lobato, Fadia V. Cervantes, Noa Míguez, Francisco J. Plou and María Fernández-Lobato
    Citation: Microbial Cell Factories 2024 23:31
  39. The global prevalence of vitamin D (VitD) deficiency associated with numerous acute and chronic diseases has led to strategies to improve the VitD status through dietary intake of VitD-fortified foods and VitD...

    Authors: Dennis Kosian, Max Willistein, Ralf Weßbecher, Constantin Eggers, Oliver May and Matthias Boll
    Citation: Microbial Cell Factories 2024 23:30
  40. Industrial by-products accrue in most agricultural or food-related production processes, but additional value chains have already been established for many of them. Crude glycerol has a 60% lower market value ...

    Authors: Tabea Helm, Thilo Stausberg, Martina Previati, Philipp Ernst, Bianca Klein, Tobias Busche, Jörn Kalinowski, Daniel Wibberg, Wolfgang Wiechert, Lien Claerhout, Nick Wierckx and Stephan Noack
    Citation: Microbial Cell Factories 2024 23:29
  41. The need to limit antibiotic therapy due to the spreading resistance of pathogenic microorganisms to these medicinal substances stimulates research on new therapeutic agents, including the treatment and preven...

    Authors: Anna Bzducha-Wróbel, Pavol Farkaš, Sandra Bieliková, Alžbeta Čížová and Marzena Sujkowska-Rybkowska
    Citation: Microbial Cell Factories 2024 23:28
  42. Pickled mustard, the largest cultivated vegetable in China, generates substantial waste annually, leading to significant environmental pollution due to challenges in timely disposal, leading to decomposition a...

    Authors: Ling Zhou, JiaZhen Sun, XiaoJun Xu, MingXia Ma, YongZhi Li, Qiao Chen and HaiFeng Su
    Citation: Microbial Cell Factories 2024 23:27
  43. In the era of rationally designed synthetic biology, heterologous metabolites production, and other counter-nature engineering of cellular metabolism, we took a step back and recalled that ‘Mother(-Nature) kno...

    Authors: Maria Gorczyca, Wojciech Białas, Jean-Marc Nicaud and Ewelina Celińska
    Citation: Microbial Cell Factories 2024 23:26
  44. Although E. coli is generally a well-opted platform for the overproduction of recombinant antigens as heterologous proteins, the optimization of expression conditions to maximize the yield of functional proteins ...

    Authors: Heba Shawky, Ashraf A. Tabll, Reem M. Elshenawy, Naiera M. Helmy, Rehab I. Moustafa, Yasser K. Elesnawy, Marwa M. Abdelghany and Yasmine S. El-Abd
    Citation: Microbial Cell Factories 2024 23:25
  45. The genus Eubacterium is quite diverse and includes several acetogenic strains capable of fermenting C1-substrates into valuable products. Especially, Eubacterium limosum and closely related strains attract atten...

    Authors: Maximilian Flaiz, Anja Poehlein, Wiebke Wilhelm, Alexander Mook, Rolf Daniel, Peter Dürre and Frank R. Bengelsdorf
    Citation: Microbial Cell Factories 2024 23:24

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