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  1. Research

    Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

    Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting bioma...

    Norma A. Valdez-Cruz, Greta I. Reynoso-Cereceda, Saumel Pérez-Rodriguez, Sara Restrepo-Pineda, Jesus González-Santana, Alejandro Olvera, Guadalupe Zavala, Alejandro Alagón and Mauricio A. Trujillo-Roldán

    Microbial Cell Factories 2017 16:129

    Published on: 25 July 2017

  2. Research

    Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis

    Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. ...

    Dinh Thi Minh Tran, Trang Thi Phuong Phan, Thanh Kieu Huynh, Ngan Thi Kim Dang, Phuong Thi Kim Huynh, Tri Minh Nguyen, Tuom Thi Tinh Truong, Thuoc Linh Tran, Wolfgang Schumann and Hoang Duc Nguyen

    Microbial Cell Factories 2017 16:130

    Published on: 25 July 2017

  3. Research

    Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi

    Lipomyces starkeyi is one of the leading lipid-producing microorganisms reported to date; its genetic transformation was only recently reported. Our aim is to engineer L. starkeyi to s...

    Qi Xu, Eric P. Knoshaug, Wei Wang, Markus Alahuhta, John O. Baker, Shihui Yang, Todd Vander Wall, Stephen R. Decker, Michael E. Himmel, Min Zhang and Hui Wei

    Microbial Cell Factories 2017 16:126

    Published on: 24 July 2017

  4. Research

    Identification of two organohalide-respiring Dehalococcoidia associated to different dechlorination activities in PCB-impacted marine sediments

    Microbial reductive dechlorination of polychlorinated biphenyls (PCBs) plays a major role in detoxifying anoxic contaminated freshwater and marine sediments from PCBs. Known members of the phylum Chloroflexi are ...

    Andrea Nuzzo, Andrea Negroni, Giulio Zanaroli and Fabio Fava

    Microbial Cell Factories 2017 16:127

    Published on: 24 July 2017

  5. Review

    Bottleneck in secretion of α-amylase in Bacillus subtilis

    Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield ...

    Shaomin Yan and Guang Wu

    Microbial Cell Factories 2017 16:124

    Published on: 19 July 2017

  6. Research

    Awakening sleeping beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase

    Propionic acid is used primarily as a food preservative with smaller applications as a chemical building block for the production of many products including fabrics, cosmetics, drugs, and plastics. Biological ...

    Ricardo Axayacatl Gonzalez-Garcia, Tim McCubbin, Annalena Wille, Manuel Plan, Lars Keld Nielsen and Esteban Marcellin

    Microbial Cell Factories 2017 16:121

    Published on: 17 July 2017

  7. Research

    Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures

    Industrial processes for recombinant protein production challenge production hosts, such as the yeast Pichia pastoris, on multiple levels. During a common P. pastoris fed-batch process, cells experience strong ad...

    Jonas Burgard, Minoska Valli, Alexandra B. Graf, Brigitte Gasser and Diethard Mattanovich

    Microbial Cell Factories 2017 16:117

    Published on: 11 July 2017

  8. Research

    Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases

    γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding o...

    Yao Zhang, Xiao Luan, Huaiyuan Zhang, Victoriano Garre, Yuanda Song and Colin Ratledge

    Microbial Cell Factories 2017 16:113

    Published on: 21 June 2017

  9. Research

    Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

    The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in t...

    Anna Gąciarz, Narendar Kumar Khatri, M. Lourdes Velez-Suberbie, Mirva J. Saaranen, Yuko Uchida, Eli Keshavarz-Moore and Lloyd W. Ruddock

    Microbial Cell Factories 2017 16:108

    Published on: 15 June 2017

  10. Research

    An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli

    The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light-driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired pheno...

    Hyun Aaron Kim, Hyun Ju Kim, Jihoon Park, Ah Reum Choi, Kyoo Heo, Haeyoung Jeong, Kwang-Hwan Jung, Yeong-Jae Seok, Pil Kim and Sang Jun Lee

    Microbial Cell Factories 2017 16:111

    Published on: 15 June 2017

  11. Research

    Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

    Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of be...

    Stephanie Brain-Isasi, Alejandro Álvarez-Lueje and Thomas Joseph V. Higgins

    Microbial Cell Factories 2017 16:110

    Published on: 15 June 2017

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