Skip to main content

The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis



Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due to high lethal rate. The T4 DNA ligase is one of the most widely utilized enzymes in modern molecular biology. Its contribution to repair chromosomal DNA damages, therefore cell survival during mutagenesis will be discussed.


Expression of T4 DNA ligase in vivo could substantially increase cell survival to ionizing radiation in multiple species. A T4 mediated survival-coupled mutagenesis approach was proposed. When polyhydroxybutyrate (PHB)-producing E. coli with T4 DNA ligase expressed in vivo was subjected to ionizing radiation, mutants with improved PHB production were acquired quickly owing to a large viable mutant library generated. Draft genome sequence analysis showed that the mutants obtained possess not only single nucleotide variation (SNV) but also DNA fragment deletion, indicating that T4 DNA ligase in vivo may contribute to the repair of DNA double strand breaks.


Expression of T4 DNA ligase in vivo could notably enhance microbial survival to excess chromosomal damages caused by various mutagens. Potential application of T4 DNA ligase in microbial mutagenesis was explored by mutating and screening PHB producing E. coli XLPHB strain. When applied to atmospheric and room temperature plasma (ARTP) microbial mutagenesis, large survival pool was obtained. Mutants available for subsequent screening for desirable features. The use of T4 DNA ligase we were able to quickly improve the PHB production by generating a larger viable mutants pool. This method is a universal strategy can be employed in wide range of bacteria. It indicated that traditional random mutagenesis became more powerful in combine with modern genetic molecular biology and has exciting prospect.


In nature, mutation combining with natural selection is the key driving force for the life evolution [1]. However, if we would like to generate microbes/organisms with desired properties, the natural evolution process is always not efficient enough and takes long time due to the low spontaneous mutation rates [2,3,4]. Therefore, varieties of mutagens either physical or chemicals such as ionizing radiation, UV radiation, alkylating agents and azides than may increase the random mutagenesis rate of the target organisms was employed in the laboratory, which provides a way to improve the mutagenesis and evolution efficiency [5].

Both chemical and physical mutagen directly or indirectly causes DNA damage, which leads to the mutation of cell genetic material. Many DNA damage response systems, such as the SOS response, is critical in mediating DNA damage repair. However, these repairing process also tend to generate mutation [6,7,8]. High dose of the mutagen could produce more DNA damage in cells, which in turn generate more mutations. However, excessive DNA damages are beyond the ability to repair cells within the mechanism that is lethal to cells. In microbial mutation breeding, the rate of lethality in the treated microbes is always more than 90% [9]. Since only viable cells can contribute to a mutation library, improving the cell survival during the mutagenesis process can greatly increase the efficiency of random mutagenesis.

With the development of molecular biology, system biology and synthetic biology, rational modification of microbes to obtain desired properties became fashionable and efficient [10,11,12]. Endogenous genes were up or down regulated to redirect the metabolic flow to desired routes, while exogenous genes were adopted to obtain the properties that was not existed in the host [13,14,15,16]. In the meantime, many approaches were developed to alter the protein expression of metabolic pathway by changing the promoter strength, enzyme stability, or even protein properties [13, 17, 18]. In this case, rational design is prevailing and efficiency are greatly improved in compare to traditional mutagenesis methods. However, the rational design is hampered greatly by current limited knowledge of complicated life and limited ability to interpret complicated cellular networks.

The T4 DNA ligase from Enterobacteria phage has been widely used in molecular biology applications in vitro given its ability of joining both sticky and blunt ended DNA [19]. In addition, T4 DNA ligase is a versatile enzyme capable of catalyzing reactions such as DNA ends relaxation [19]; duplex DNA gap sealing [20]; ligation of DNA with base pair mismatched [21]; nick-closing [22] and oligomerization of bacteriophage [23, 24].

Previous studies have shown that DNA ligase contributes to chromosome DNA nicks repair after in vivo scission by restriction endonuclease [25, 26]. In our recent study, we found T4 DNA ligase in vivo was able to mediate the repair of DNA double strands breaks (DSBs) (Manuscript accepted). This not only increased the survival of the host but also increased the mutation if we treated the microbes with traditional DSBs causing method, for example, random mutagenesis machine atmospheric and room temperature plasma (ARTP-a microbial mutagenesis machine that causes DNA sequences changes). Based on this, a new method called T4 mediated survival-coupled mutagenesis (T4SM) was proposed, which combined the traditional mutagenesis and molecular biology method by in vivo expression T4 DNA ligase to improve the mutagenesis survival of the host. The efficiency of the method was demonstrated using a PHB-producing E. coli strain. The obtained mutants were sequenced to gain an understanding between phenotypes and genomic variations.

Results and discussion

T4 DNA ligase in vivo increases cell survival to genotoxic drug treatment

In an initial investigation, we found that T4 DNA ligase in vivo mediates the repair of DNA double breaks in an error and prone manner (Manuscript accepted). In the meantime, we did not observe any physiological change of the host when T4 DNA ligase was over-expressed in E. coli. To see if the genotoxic drugs, which may kill the bacteria by inducing the DNA double breaks in vivo, may affect the cell growth, ciprofloxacin was adopted. As shown in Fig. 1, both E. coli (VC) and E. coli (T4) strains can grow normally at 25 μg/mL ciprofloxacin. When ciprofloxacin increased to 50 μg/mL, wild type E. coli MG1655 cannot grow in liquid culture up to 24 h incubation, but the growth of E. coli (T4) was detected after 14 h, indicating that ciprofloxacin resistant mutants arising. In presence of 75 μg/mL and 100 μg/mL of ciprofloxacin, growth was also detected after about 14–20 h incubation in T4 ligase expressed strain (Fig. 1). Since ciprofloxacin causes chromosome DNA DSBs through inhibiting DNA gyrase and topoisomerase IV [27], this observation clearly indicated that expression of T4 DNA ligase in vivo contributes to chromosomal DNA repair, possibly DSBs repair, which in turn increases cell survival. The increased survival further improved survival-coupled mutagenesis, among which resistant mutants arise on the chromosome ultimately.

Fig. 1
figure 1

T4 DNA ligase increases host cell survival to ciprofloxacin treatment. Growth curve of either E. coli (VC) or E. coli (T4) in presence of 25, 50, 75 and 100 μg/mL ciprofloxacin respectively (Y-axis: OD600; X-axis time (h)). Experiments were performed in triplicates

T4 DNA ligase in vivo increases cell resistance to ionizing radiation

To see if the cell expressing the T4 DNA ligase may also resistant to other DSBs causing factors, a new type of random mutagenesis machine ARTP, which causes various chromosomal damages including lethally DSBs, was employed [28]. To evaluate the contribution of T4 DNA ligase to host cell survival to ionizing radiation, cell survival of E. coli (VC) and E. coli (T4) was measured by CFU counts after ARTP exposure. As ionizing radiation time increased, survival of E. coli (VC) decreased correspondingly (Fig. 2a). After 30 s ARTP treatment, almost no survival was observed (Fig. 2a, b). Over increased ionizing radiation time, survival rate of E. coli (T4) dropped as vector control group did. Under same ionizing radiation time, however, E. coli (T4) showed a fivefold increase in survival to 10 s ARTP treatment in comparison to E. coli (VC) and cell survived up to 40 s ARTP treatment (Fig. 2a, b). This result clearly showed that T4 DNA ligase in vivo also increases the cell resistance to ionizing radiation, possibly via mediating the DSB repair caused by ARTP treatment.

Fig. 2
figure 2

T4 DNA ligase confers cell survival to ionizing radiation (a). Survival of E. coli (VC) and E. coli (T4) to ARTP radiation. Equal number cells of E. coli MG1655 harboring empty vector (VC) or T4 DNA ligase expressing plasmid (T4 DNA ligase) were subjected to ARTP radiation for 10 s, 20 s, 30 s and 40 s, respectively. The resulting cells were plated onto agar plates to count CFU. An asterisk (*) stands for statistically significant difference (p < 0.001, unpaired t-test). b Cell survival of E. coli (VC) and E. coli (T4) upon ARTP exposure

T4 DNA ligase mediated survival-coupled mutagenesis

Based on above results, a T4 mediated survival-coupled mutagenesis (T4SM) approach was proposed. When mutagens were used to mutagenize microbes for desired purpose, T4 DNA ligase can be employed and expressed in the host to repair chromosomal damages caused by mutagens. The host with repaired chromosome DSBs by T4 DNA ligase may survive for longer time and accumulate more mutations during the mutagen treatment. This will result in more mutations in one strain and a large survival pool for screening during the mutagenesis process.

To explore the feasibility of T4 DNA ligase-mediated microbial mutagenesis, the polyhydroxybutyrate (PHB) producing E. coli XLPHB, which harbors chromosomal integrated PHB biosynthesis genes phbCAB from Ralstonia eutropha, was subjected to mutagenesis and screening with T4 DNA ligase expressed in vivo [12]. Generally, there was nearly no colony on the plate after the cells were treated by ARTP for 60-s (Additional file 1: Fig. S1). However, expression of T4 DNA ligase in XLPHB increased host cell survival to 5 s ARTP radiation by five to sixfold (Additional file 1: Fig. S1a). Extended ARTP treatment obtained even more obvious result. This result proved the effectiveness of the T4SM method: the presence of T4 DNA ligase increased the survival rate and provided more mutated strains for further screening.

To estimate mutants acquired, ten colonies were then randomly selected and analyzed for their PHB production. As shown in Fig. 3, the control strain E. coli XLPHB accumulated 25.36 ± 2.82% w/w of the cell dry weight PHB. Mutants obtained by ARTP treatment vary dramatically in terms of PHB content. The highest PHB content was found to accumulate 112% more in comparison with the control and reached 53.87 ± 1.11% w/w of the cell dry weight. It indicates the methodology produces a library of mutants with diverse phenotypes.

Fig. 3
figure 3

PHB fermentation results of E. coli XLPHB and ten randomly selected T4SM mutants

Fig. 4
figure 4

T4 mediated survival-coupled mutagenesis (T4SM) is applicable to P. putida and L. plantarum. a Survival of P. putida to ARTP radiation. Equal number cells of P. putida (VC) or P. putida (T4) were subjected to ARTP radiation for 5 s, 10 s, 15 s and 30 s respectively. The resulting cells were plated onto agar plates to count CFU. b Survival of L. plantarum to ARTP radiation. Equal number cells of L. plantarum (VC) or L. plantarum (T4) were subjected to ARTP radiation for 15 s, 30 s, 45 s and 60 s respectively. The resulting cells were plated onto agar plates to count CFU. Data shown are representative of three replicates and standard deviations were presented as error bars. An asterisk (*) stands for statistically significant difference (p < 0.001, unpaired t-test)

Two of the obtained mutants PHB-6 and PHB-10 with increased or decreased PHB accumulation were randomly selected and sent for draft genome sequencing. The results revealed both DNA fragment deletion and single nucleotide variation (SNV) mutation after ARTP treatment (Table 1). For mutant PHB-6, a 13-bp deletion was introduced inside the open reading frame of yieK gene, inactivating the putative 6-phosphogluconolactonase it encodes for. Inactivation of 6-phosphogluconolactonase may lead to the accumulation of 6-phosphogluconalactone, the product of glucose-6-phosphate oxidation and increase the supply of glucose-6-phospahte for glycolysis. Correspondingly, PHB-6 with mutated yieK increased PHB content from 25.36 ± 2.82% w/w to 39.47 ± 1.00%. This indicates that yieK is a novel target that has not been investigated and can potentially increase the carbon flux to glycolysis. In another sequenced mutant PHB-10, a 65 bp deletion residing in the ORF of ilvB gene, which encodes the acetoacetate synthase I subunit was found [29]. In addition, there was a 1-bp deletion between two ArcA-regulated repression sites upstream of sdhC promoter in this mutant [30]. Mutation of this site may interfere with the transcription of succinate dehydrogenase, the major component of the respiration chain. The very low cell mass of 2.72 ± 0.49 g/L and little PHB accumulation (1.95 ± 0.14%) of this mutant confirmed this prediction and draft sequencing result (Table 1). All these suggested that T4SM could greatly accelerate microbial breeding process by providing large mutants library. Mutants acquired by this approach bear mutation of SNV and short deletions.

Table 1 Identified mutations of PHB-6 and PHB-10

T4 DNA ligase mediated survival-coupled mutagenesis is applicable to both Gram-positive and Gram-negative bacteria

Contribution of T4 DNA ligase to host cell survival was also tested in Gram-negative bacterium P. putida and gram-positive bacterium L. plantarum. In P. putida, cell survival rate dropped correspondingly over time with radiation treatment (Fig. 4a). When T4 DNA ligase was expressed, the P. putida (T4) showed fivefold increased survival to ARTP treatment compared to that of P. putida (VC). Similar pattern was also observed in L. plantarum, in which T4 DNA ligase expression (L. plantarum (T4)) showed a fourfold increase after ARTP treatment compared to the control L. plantarum (VC) (Fig. 4b). These results suggested that T4 DNA ligase mediated survival to irradiation is applicable to wide range of bacteria either Gram-negative or Gram-positive and could be valuable for improving the random mutagenesis efficiency of these bacteria.


In this study, we demonstrated that expression of T4 DNA ligase in vivo increase host cell survival to DSBs causing factors, such as genotoxic drugs and ionizing radiation. Based on this, T4 mediated survival-coupled mutagenesis (T4SM) was proposed, of which the effectiveness was validated by rapidly improving the PHB production in E. coli using ARTP treatment. Combined with high throughput screening methodology, the obtained mutants by the T4 mediated survival-coupled mutagenesis (T4SM) can now be sequenced to gain insights to the links between phenotypes and genomic variations. This indicated that traditional random mutagenesis shall be more powerful in combine with the modern genetic molecular biology and has exciting prospect.

Materials and methods

Bacterial strains and culture conditions

All bacteria strains are lab stocks and listed in Table 2. All E. coli and P. putida S16 strains were routinely cultured in Luria–Bertani (LB) broth with aeration at 220 rpm at 37 °C or 30 °C as indicated. The L. plantarum WCFS1 was routinely cultured in deMan Rogosa Sharpe (MRS) broth without aeration at stationary culture at 37 °C. Antibiotics were added to the following concentration when needed: spectinomycin (50 μg/mL), kanamycin (50 mg/mL) and erythromycin (250 μg/mL for E. coli and 25 μg/mL for L. plantarum). E. coli DH5α strain was used for molecular cloning and plasmids propagation. Engineered PHB producing E. coli XLPHB strain was subjected to ARTP treatment [12, 31].

Table 2 Bacterial strains and plasmids used in the study

Plasmid construction

All plasmid used in this study are listed in Table 2. All primers used in this study are listed in Additional file 1: Table S1. The pUCLR4 plasmid was assembled from the LR4 spacer amplified from previously reported plasmid p15A-L4 [35] using primers gRNA Spc-F/gRNA Spc-R, the pLtet promoter amplified from pwtCas9 plasmid using pLtet-F/pLtet-R and pUC Ori amplified from pUC19 plasmid using Ori-F/Ori-R by Gibson assembly [37].

Plasmid pUCLR4-T4 was assembled from T4 DNA ligase gene amplified from Enterobacteria phage T4 using primers T4-F/T4-R and pUCLR4 backbone amplified using primers T4 (ori terminator)-F/Ori (terminator)-R by Gibson assembly [37]. T4 DNA ligase gene was cloned under the control of a constitutive PJ23104 promoter (

To express T4 DNA ligase in P. putida S16, the T4 DNA ligase gene with an upstream constitutive PJ23104 promoter was amplified from pUCLR-T4 plasmid using primers T4 HindIII-F/T4 KpnI-R and cloned into vector pBBR1MCS-2 at HindIII and KpnI sites, resulting in plasmid pBBR-T4.

To express T4 DNA ligase in L. plantarum, plasmid pE-T4 was assembled from the T4 DNA ligase gene with an upstream constitutive PJ23104 promoter amplified from pUCLR-T4 plasmid using primers T4 pE-F/T4 pE-R and the pE plasmid backbone amplified using primers pE T4-F/pE T4-R by Gibson assembly [37].

Bacterial transformation

Escherichia coli transformation was performed by chemical transformation. Transformation of L. plantarum by electroporation was performed as previous described [38]. Briefly, L. plantarum cells cultured to mid-exponential phase (OD600 ~ 0.4–0.6) were collected, washed twice with SM buffer (952 mM sucrose supplemented with 3.5 mM MgCl2) and resuspended in SM buffer. Plasmid DNA ( ~ 1 μg) was added to 100 μL prepared competent cells. The resulting cell mixture were incubated on ice for 10 min. Cell mixture were electroporated using 2 mm electroporation cuvette and Gene Pulser (BioRad) under following condition: 2000 V, 25 μF, 400 Ω. Cells was recovered in SMRS broth (MRS broth supplemented with 0.5 M sucrose and 0.1 M MgCl2) at 37 °C for 3 h before spreading on MRS agar plates containing erythromycin.

Transformation of P. putida by electroporation following previously published protocol [39]. P. putida cells cultured to mid- exponential phase (OD600 ~ 0.2–0.4) were collected, washed twice with 300 mM sucrose solution and resuspended in 300 mM sucrose. After 10 min incubation on ice, the mixture of competent cells and DNA ( ~ 1 μg) was electroporated using 2 mm electroporation cuvette and Gene Pulser (BioRad) under following condition: 2500 V, 25 μF, 400 Ω. Cells was recovered in LB broth at 30 °C for 2 h before spreading on LB agar plates containing kanamycin.

Ciprofloxacin sensitivity assay

Overnight culture of E. coli MG1655 harboring either plasmid pUCLR4 or pUCLR4-T4 was sub-cultured to fresh LB medium supplemented with spectinomycin. Subcultures were transferred to 24-well microtiter plate. Ciprofloxacin was added to cultures to final concentrations of 25 μg/mL, 50 μg/mL, 75 μg/mL or 100 μg/mL, respectively. Growth was monitored by measuring OD600 every hour for 48 h using plate reader (BioTek Synergy HT) at 37 °C with shaking.

Atmospheric and room temperature plasma treatment

Bacterial strains were collected when grown to mid-log phase and washed by NaCl solution (normal saline) twice. Equal number of cells were re-suspended in NaCl solution to a standard OD600 (1.0). For each sample, 10 μL of the resulting cultures was spread onto stainless disc. The sample disc was placed 2 mm below the plasma torch nozzle exit. All ARTP irradiation treatments were performed using atmospheric and room temperature plasma (ARTPII, Tmaxtree Biotechnology) with radiofrequency power input at 100 W and gas flow at 10 SLM (standard liters per minute) at room temperature (15–30 °C). Immediately after the radiation, 1 mL of NaCl solution was added to the sample disc to re-suspend bacteria by vortex. CFU was counted for the resulting samples. Samples treated the same while lacking radiation was plated and analyzed as control groups.

PHB fermentation and content determination

Sample of E. coli XLPHB treated with ARTP was plated onto CongoRed agar to identify PHB producers. Single colony of each mutant tested was inoculated into 5 mL LB medium and cultured for 12 h at 37 °C with aeration at 180 rpm. Cultures were sub-cultured at 4:100 ratio to fresh 50 mL LB medium with 30 g/L glucose at 37 °C with aeration at 180 rpm. Cells were collected after 48 h of fermentation and freeze-dried for 12 h (the weight of total dried cells is measured as Mx). PHB content of 10–20 mg (mx) freeze-dried cells were measured by gas chromatography after methanolysis as previously described [40]. Collected dried cells for PHB content analysis was weight as mx. The PHB content was calculated as m(PHB)/mx (stands for content of PHB per mg dried cells); total production of PHB was calculated as m(PHB) * Mx/mx.

Genome DNA sequencing and analysis

Genome DNA of desired sample was extracted using TIANamp Bacteria DNA Kit (Tiangen, China) and sequenced by GENEWIZ. The resequencing method was used for the analysis of single nucleotide variant (SNV), insertion and deletion (INDEL) as well as structure variant. Genome of E. coli MG1655 was used as the reference genome (

Statistical analysis

All experiments were performed three times in triplicates. Data shown were representative of three biological replicates with standard deviation as error bars.

Availability of data and materials

Not applicable.



atmospheric and room temperature plasma




double strand break


  1. Elena SF, Lenski RE. Evolution experiments with microorganisms: the dynamics and genetic bases of adaptation. Nat Rev Genet. 2003;4(6):457–69.

    Article  CAS  Google Scholar 

  2. Lee H, Popodi E, Tang H, Foster PL. Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing. Proc Natl Acad Sci USA. 2012;109(41):E2774–E27832783.

    Article  CAS  Google Scholar 

  3. Lynch M. Evolution of the mutation rate. Trends Genet. 2010;26(8):345–52.

    Article  CAS  Google Scholar 

  4. Drake JW. A constant rate of spontaneous mutation in DNA-based microbes. Proc Natl Acad Sci USA. 1991;88(16):7160–4.

    Article  CAS  Google Scholar 

  5. Kodym A, Afza R. Physical and chemical mutagenesis. Methods Mol Biol. 2003;236:189–204.

    CAS  PubMed  Google Scholar 

  6. Krishna S, Maslov S, Sneppen K. UV-induced mutagenesis in Escherichia coli SOS response: a quantitative model. PLoS Comput Biol. 2007;3(3):e41.

    Article  Google Scholar 

  7. Schofield MJ, Hsieh P. DNA mismatch repair: molecular mechanisms and biological function. Annu Rev Microbiol. 2003;57:579–608.

    Article  CAS  Google Scholar 

  8. Smith BT, Walker GC. Mutagenesis and more: umuDC and the Escherichia coli SOS response. Genetics. 1998;148(4):1599–610.

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Zhang X, Zhang C, Zhou Q-Q, Zhang X-F, Wang L-Y, Chang H-B, et al. Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis. Appl Microbiol Biotechnol. 2015;99(13):5639–46.

    Article  CAS  Google Scholar 

  10. Prather KLJ, Martin CH. De novo biosynthetic pathways: rational design of microbial chemical factories. Curr Opin Biotechnol. 2008;19(5):468–74.

    Article  Google Scholar 

  11. McNerney MP, Watstein DM, Styczynski MP. Precision metabolic engineering: the design of responsive, selective, and controllable metabolic systems. Metab Eng. 2015;31:123–31.

    Article  CAS  Google Scholar 

  12. Zhang X, Zhang J, Xu J, Zhao Q, Wang Q, Qi Q. Engineering Escherichia coli for efficient coproduction of polyhydroxyalkanoates and 5-aminolevulinic acid. J Ind Microbiol Biotechnol. 2018;45(1):43–51.

    Article  CAS  Google Scholar 

  13. Kang Z, Wang Y, Gu P, Wang Q, Qi Q. Engineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose. Metab Eng. 2011;13(5):492–8.

    Article  CAS  Google Scholar 

  14. He X, Chen Y, Liang Q, Qi Q. Autoinduced AND gate controls metabolic pathway dynamically in response to microbial communities and cell physiological state. ACS Synth Biol. 2017;6(3):463–70.

    Article  CAS  Google Scholar 

  15. Cui Z, Gao C, Li J, Hou J, Lin CSK, Qi Q. Engineering of unconventional yeast Yarrowia lipolytica for efficient succinic acid production from glycerol at low pH. Metab Eng. 2017;42:126–33.

    Article  CAS  Google Scholar 

  16. Paddon CJ, Westfall PJ, Pitera DJ, Benjamin K, Fisher K, McPhee D, et al. High-level semi-synthetic production of the potent antimalarial artemisinin. Nature. 2013;496(7446):528–32.

    Article  CAS  Google Scholar 

  17. Yang P, Wang J, Pang Q, Zhang F, Wang J, Wang Q, et al. Pathway optimization and key enzyme evolution of N-acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor. Metab Eng. 2017;43(Pt A):21–8.

    Article  CAS  Google Scholar 

  18. Boyle PM, Silver PA. Parts plus pipes: synthetic biology approaches to metabolic engineering. Metab Eng. 2012;14(3):223–32.

    Article  CAS  Google Scholar 

  19. Rossi R, Montecucco A, Ciarrocchi G, Biamonti G. Functional characterization of the T4 DNA ligase: a new insight into the mechanism of action. Nucleic Acids Res. 1997;25(11):2106–13.

    Article  CAS  Google Scholar 

  20. Mueser TC, Hinerman JM, Devos JM, Boyer RA, Williams KJ. Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives. Virol J. 2010;7:359.

    Article  CAS  Google Scholar 

  21. Yuan C, Lou XW, Rhoades E, Chen H, Archer LA. T4 DNA ligase is more than an effective trap of cyclized dsDNA. Nucleic Acids Res. 2007;35(16):5294–302.

    Article  CAS  Google Scholar 

  22. Ciarrocchi G, Lestingi M, Wright G, Montecucco A. Bacteriophage T4 and human type I DNA ligases relax DNA under joining conditions. Nucleic Acids Res. 1993;21(25):5934–9.

    Article  CAS  Google Scholar 

  23. Cherepanov A, Yildirim E, de Vries S. Joining of short DNA oligonucleotides with base pair mismatches by T4 DNA ligase. J Biochem. 2001;129(1):61–8.

    Article  CAS  Google Scholar 

  24. Nilsson SV, Magnusson G. Sealing of gaps in duplex DNA by T4 DNA ligase. Nucleic Acids Res. 1982;10(5):1425–37.

    Article  CAS  Google Scholar 

  25. Heitman J, Zinder ND, Model P. Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease. Proc Natl Acad Sci. 1989;86(7):2281–5.

    Article  CAS  Google Scholar 

  26. Heitman J, Ivanenko T, Kiss A. DNA nicks inflicted by restriction endonucleases are repaired by a RecA- and RecB-dependent pathway in Escherichia coli. Mol Microbiol. 1999;33(6):1141–51.

    Article  CAS  Google Scholar 

  27. Wilkinson M, Troman L, Wan Nur Ismah WA, Chaban Y, Avison MB, Dillingham MS, et al. Structural basis for the inhibition of RecBCD by Gam and its synergistic antibacterial effect with quinolones. Elife. 2016;5:e22963.

    Article  Google Scholar 

  28. Zhang X, Zhang X-F, Li H-P, Wang L-Y, Zhang C, Xing X-H, et al. Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool. Appl Microbiol Biotechnol. 2014;98(12):5387–96.

    Article  CAS  Google Scholar 

  29. Weinstock O, Sella C, Chipman DM, Barak Z. Properties of subcloned subunits of bacterial acetohydroxy acid synthases. J Bacteriol. 1992;174(17):5560–6.

    Article  CAS  Google Scholar 

  30. Nakamura K, Yamaki M, Sarada M, Nakayama S, Vibat CR, Gennis RB, et al. Two hydrophobic subunits are essential for the heme b ligation and functional assembly of complex II (succinate-ubiquinone oxidoreductase) from Escherichia coli. J Biol Chem. 1996;271(1):521–7.

    Article  CAS  Google Scholar 

  31. Zhang X, Zhang J, Xu J, Zhao Q, Wang Q, Qi Q. Engineering Escherichia coli for efficient coproduction of polyhydroxyalkanoates and 5-aminolevulinic acid. J Ind Microbiol Biotechnol. 2018;45:43–51.

    Article  CAS  Google Scholar 

  32. Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, et al. The complete genome sequence of Escherichia coli K-12. Science. 1997;277(5331):1453–62.

    Article  CAS  Google Scholar 

  33. Yu H, Tang H, Wang L, Yao Y, Wu G, Xu P. Complete genome sequence of the nicotine-degrading Pseudomonas putida strain S16. J Bacteriol. 2011;193(19):5541–2.

    Article  CAS  Google Scholar 

  34. Kleerebezem M, Boekhorst J, van Kranenburg R, Molenaar D, Kuipers OP, Leer R, et al. Complete genome sequence of Lactobacillus plantarum WCFS1. Proc Natl Acad Sci USA. 2003;100(4):1990–5.

    Article  CAS  Google Scholar 

  35. Su T, Liu F, Gu P, Jin H, Chang Y, Wang Q, et al. A CRISPR-Cas9 assisted non-homologous end-joining strategy for one-step engineering of bacterial genome. Sci Rep. 2016;6:37895.

    Article  CAS  Google Scholar 

  36. Sørvig E, Mathiesen G, Naterstad K, Eijsink VGH, Axelsson L. High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors. Microbiology. 2005;151(Pt 7):2439–49.

    Article  Google Scholar 

  37. Gibson DG, Young L, Chuang R-Y, Venter JC, Hutchison CA, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009;6(5):343–5.

    Article  CAS  Google Scholar 

  38. Yang P, Wang J, Qi Q. Prophage recombinases-mediated genome engineering in Lactobacillus plantarum. Microb Cell Fact. 2015;14:154.

    Article  Google Scholar 

  39. Iwasaki K, Uchiyama H, Yagi O, Kurabayashi T, Ishizuka K, Takamura Y. Transformation of Pseudomonas putida by electroporation. Biosci Biotechnol Biochem. 1994;58(5):851–4.

    Article  CAS  Google Scholar 

  40. Wang Q, Yu H, Xia Y, Kang Z, Qi Q. Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application. Microb Cell Fact. 2009;8:47.

    Article  Google Scholar 

Download references


Not applicable.


This work was supported by grants from the National Natural Science Foundation of China [31730003, 31670077] and Natural Science Foundation of Shandong Province [ZR2017ZB0210].

Author information

Authors and Affiliations



JW and FL contributed equally to this work. FL performed experiments and analyzed data. JW, TS, YC and QG performed some experiments and analyzed data. YC analyzed genome sequencing data. QW and QL contributed to analyze and interpret data. QQ and JW wrote manuscript. QQ conceived and supervised the study. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Qingsheng Qi.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Additional file

Additional file 1: Table S1.

Primers used in this study.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wang, J., Liu, F., Su, T. et al. The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis. Microb Cell Fact 18, 107 (2019).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • T4 DNA ligase
  • Microbial mutagenesis
  • Atmospheric and room temperature plasma (ARTP)
  • Polyhydroxybutyrate (PHB)