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Fig. 4 | Microbial Cell Factories

Fig. 4

From: The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis

Fig. 4

T4 mediated survival-coupled mutagenesis (T4SM) is applicable to P. putida and L. plantarum. a Survival of P. putida to ARTP radiation. Equal number cells of P. putida (VC) or P. putida (T4) were subjected to ARTP radiation for 5 s, 10 s, 15 s and 30 s respectively. The resulting cells were plated onto agar plates to count CFU. b Survival of L. plantarum to ARTP radiation. Equal number cells of L. plantarum (VC) or L. plantarum (T4) were subjected to ARTP radiation for 15 s, 30 s, 45 s and 60 s respectively. The resulting cells were plated onto agar plates to count CFU. Data shown are representative of three replicates and standard deviations were presented as error bars. An asterisk (*) stands for statistically significant difference (p < 0.001, unpaired t-test)

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