Table 2 Bacterial strains and plasmids used in the study
From: The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
Strain | Description | Source or reference |
|---|---|---|
Bacterial strains | ||
 E. coli MG1655 | F−, lambda−, rph-1 | [32] |
 E. coli XLPHB | DH5αΔpoxB::M(p5tac-phbCAB-kan) | [12] |
 Pseudomonas putida S16 | Wild type | [33] |
 Lactobacillus plantarum WCFS1 | Wild type | [34] |
 E. coli DH5α | F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen |
 E. coli (VC) | E. coli MG1655 strain harboring plasmid pUCLR4 | This work |
 E. coli (T4) | E. coli MG1655 strain harboring plasmid pUCLR4-T4 | This work |
 XLPHB (VC) | E. coli XLPHB strain harboring plasmid pUCLR4 | This work |
 XLPHB (T4) | E. coli XLPHB strain harboring plasmid pUCLR4-T4 | This work |
 P. putida (VC) | P. putida S16 strain harboring plasmid pBBR1MCS-2 | This work |
 P. putida (T4) | P. putida S16 strain harboring plasmid pBBR-T4 | This work |
 L. plantarum (VC) | L. plantarum WCFS1 strain harboring plasmid pE | This work |
 L. plantarum (T4) | L. plantarum WCFS1 strain harboring plasmid pE-T4 | This work |
Plasmids | ||
 p15A-L4 | Low copy number cloning vector, P15A origin of replication | [35] |
 pUCLR4 | High copy number cloning vector, pUC origin of replication, SpeR, | This work |
 pUCLR4-T4 | pUCLR4 derivative, T4 DNA ligase expressed from a constitutive PJ23104 promoter | This work |
 pBBR1MCS-2 | Broad host range cloning vector, pBBR1 origin of replication, KanR, low copy number | [33] |
 pBBR-T4 | pBBR1MCS-2 derivative; T4 DNA ligase gene, expressed from a constitutive PJ23104 promoter | This work |
 pE | E. coli-L. plantarum shuttle vector, pUC origin of replication, 256 origin of replication (low copy number), ErmR | [36] |
 pE-T4 | pE derivative, T4 DNA ligase gene, expressed from a constitutive PJ23104 promoter | This work |