- Open Access
Switching the mode of sucrose utilization by Saccharomyces cerevisiae
© Badotti et al; licensee BioMed Central Ltd. 2008
- Received: 18 November 2007
- Accepted: 27 February 2008
- Published: 27 February 2008
Overflow metabolism is an undesirable characteristic of aerobic cultures of Saccharomyces cerevisiae during biomass-directed processes. It results from elevated sugar consumption rates that cause a high substrate conversion to ethanol and other bi-products, severely affecting cell physiology, bioprocess performance, and biomass yields. Fed-batch culture, where sucrose consumption rates are controlled by the external addition of sugar aiming at its low concentrations in the fermentor, is the classical bioprocessing alternative to prevent sugar fermentation by yeasts. However, fed-batch fermentations present drawbacks that could be overcome by simpler batch cultures at relatively high (e.g. 20 g/L) initial sugar concentrations. In this study, a S. cerevisiae strain lacking invertase activity was engineered to transport sucrose into the cells through a low-affinity and low-capacity sucrose-H+ symport activity, and the growth kinetics and biomass yields on sucrose analyzed using simple batch cultures.
We have deleted from the genome of a S. cerevisiae strain lacking invertase the high-affinity sucrose-H+ symporter encoded by the AGT1 gene. This strain could still grow efficiently on sucrose due to a low-affinity and low-capacity sucrose-H+ symport activity mediated by the MALx1 maltose permeases, and its further intracellular hydrolysis by cytoplasmic maltases. Although sucrose consumption by this engineered yeast strain was slower than with the parental yeast strain, the cells grew efficiently on sucrose due to an increased respiration of the carbon source. Consequently, this engineered yeast strain produced less ethanol and 1.5 to 2 times more biomass when cultivated in simple batch mode using 20 g/L sucrose as the carbon source.
Higher cell densities during batch cultures on 20 g/L sucrose were achieved by using a S. cerevisiae strain engineered in the sucrose uptake system. Such result was accomplished by effectively reducing sucrose uptake by the yeast cells, avoiding overflow metabolism, with the concomitant reduction in ethanol production. The use of this modified yeast strain in simpler batch culture mode can be a viable option to more complicated traditional sucrose-limited fed-batch cultures for biomass-directed processes of S. cerevisiae.
- Ethanol Production
- Biomass Yield
- Sucrose Uptake
- AGT1 Gene
- Overflow Metabolism
The yeast Saccharomyces cerevisiae has been known to humans for thousands of years and it is routinely used in many traditional biotechnological processes, including bread making and production of several alcoholic beverages. Consequently, it has been extensively studied and thus is considered a model system for the metabolic, molecular and genetic analysis of eukaryotic organisms. Due to its GRAS status, S. cerevisiae yeasts are also applied on a huge scale in biomass-directed processes, such as the production of baker's yeast, yeast extract and other food additives (vitamins, proteins, enzymes, and flavouring agents) , as well as for production of heterologous proteins (including vaccines and other therapeutic compounds), or even for engineering completely novel metabolic pathways leading to the biotechnological production of important pharmaceuticals [2–5]. The combination of the large knowledge of yeast physiology, together with the fact that the yeast genome has been fully sequenced, has resulted in the development of production strains with optimized properties [6, 7].
However, it should be stressed out that most of the industrial applications of S. cerevisiae rely in its ability to efficiently ferment sugars, even under fully aerobic conditions . Since low by-product formation and a high biomass yield on sugar are prerequisites for the economic viability of biomass-directed applications, the occurrence of alcoholic fermentation in such processes is highly undesirable as it will reduce the biomass yield . Aerobic ethanol production by S. cerevisiae cultures occurs when the carbon flux through glycolysis exceeds the capacity of the tricarboxylic acids cycle to completely oxidize the pyruvate produced. Thus, fully respiratory metabolism only takes place during the utilization of low sugar concentrations and slow rates of sugar consumption and growth, and plenitude of oxygen. Indeed, this yeast has developed several sensing and signaling mechanisms in order to not only ensure efficient sugar uptake from the medium, but to also repress alternative carbon source utilization and respiration, thus favoring the production of ethanol [10–14]. Accordingly, high cell concentrations are rarely feasible in a simple batch mode, as the required high initial sugar concentration would result in the significant production of ethanol, which can accumulate to values as high as 50% of the supplied sugar.
Consequently, in order to maximize biomass yield S. cerevisiae yeast cell are cultivated in a fed-batch manner, in which a sugar-concentrated solution is fed into the bioreactor under a variety of control strategies. Usually, after a batch phase, an exponential feeding profile is applied to ensure optimal production and growth conditions, followed by a decline phase at the end of cultivation. To ensure optimal oxidative growth several approaches have been developed to control the feed rate at a level below the critical value, beyond which ethanol is produced and therefore the biomass yield decreases. Nevertheless, supplementary equipment, complex control systems and kinetic models are usually required to monitor on-line the fermentation process in order to provide small sugar concentrations to the yeast cells, avoiding ethanol production [15–17]. Other technical and physical limitations, such as time delays or measurement noise, sub-optimal stirring and oxygen transfer, as well as non-homogenous supply of nutrients, may result in both a decrease of the growth rate of the microorganism and/or overflow metabolism, and consequently decreases in biomass productivity [18, 19]. Many of these problems could be overcome by culturing the cells in the simpler batch mode, as long as overflow metabolism and/or ethanol production is prevented.
Sucrose is by far the most abundant, cheap and important sugar in the industrial utilization of the yeast S. cerevisiae. More than half of the world's ethanol production relies on the efficient fermentation of sucrose-rich broths such as sugarcane juice and molasses, and these raw materials are also used for the production of baker's yeast, and for production of several distilled alcoholic beverages [20, 21]. It is generally accepted that S. cerevisiae cells harbor an extracellular invertase (β-D-fructosidase), that hydrolyzes sucrose into glucose and fructose, which are transported into the cell by hexose transporters and metabolized through glycolysis. This enzyme has been a paradigm for the study of protein synthesis and regulation of gene expression. Invertase is encoded by one or several SUC genes (SUC1 to SUC5 and SUC7), SUC2 being the most common loci found in almost all S. cerevisiae strains, including in other closely related yeast species [22, 23].
These SUC genes generate two different mRNAs: a larger transcript encoding an invertase with a signal sequence required for its secretion from the cell, and a shorter transcript lacking this signal sequence and thus coding for an intracellular form of the enzyme . While the former mRNA is repressed by high concentrations of sucrose or its hydrolysis products (glucose and fructose), the intracellular invertase is expressed constitutively. Finally, it has recently become evident that efficient invertase expression requires low levels of glucose or fructose in the medium [25–27]. Despite significant improvements in our knowledge regarding the molecular mechanisms involved in the repression of SUC expression, the transcriptional activator of this gene is still unknown [28, 29]. A further level of complexity is the fact that invertase levels at the yeast surface are poorly (or even inversely) correlated with the ability of the cells to ferment this sugar, especially at high sucrose concentrations [30–32]. Extracellular sucrose hydrolysis may even allow growth of other microorganisms, including contaminant yeasts lacking invertase . Extracellular production of fructose also imposes several problems to the industrial process due to slower fructose utilization by S. cerevisiae cells , which may result in residual sugar at the end of the cultivation with consequent losses in productivity.
In this study, a poorly characterized pathway for sucrose utilization in S. cerevisiae was engineered in order to improve biomass-directed applications of this microorganism. Several reports have shown that the kinetics of cell growth on sucrose by this yeast can only fit a model in which its utilization is composed of the contributions from both the direct uptake of sucrose, and the uptake of its hydrolysis products into the cell [35–37]. The analysis of direct sucrose uptake by S. cerevisiae cells revealed the presence of an active sucrose-H+ symport [38, 39] which was shown to be mediated by two different transport systems: high-affinity (Km~7 mM) uptake mediated by the the AGT1 permease, while the MALx1 maltose transporters allow the active uptake of sucrose with low (Km > 100 mM) affinity [40, 41]. The active uptake of sucrose would justify the existence of the constitutive intracellular invertase, although sucrose can also be hydrolyzed by other intracellular glycosidases, such as α-glucosidase (maltase), an enzyme with the same affinity and activity for sucrose and maltose . Indeed, we have recently shown that yeast strains unable to ferment glucose or fructose due to the absence of hexose transport, or strains lacking invertase, can actively transport sucrose into the cells allowing efficient fermentation of this sugar [43, 44]. In the present report we show that when we modulate at the molecular level the rate of active sucrose uptake, we obtain yeast strains that can easily attain higher cell densities when grown in simple batch cultures with 20 g/L sucrose as carbon source. This novel and value strategy that improves one of the industrial applications of S. cerevisiae represents an interesting alternative to classical bioprocessing approaches.
Kinetics of active H+-sucrose uptake in yeast
In order to develop a new yeast strain that would take up sucrose from the medium by just the low-affinity transport activity, we deleted from strain's 1403-7A genome the high-affinity sucrose-H+ symporter encoded by the AGT1 gene (see strain LCM001 in Fig. 2). As expected, the kinetics of active sucrose transport indicated that in the agt1 Δ strain LCM001 sucrose-H+ symport was mediated by a low-affinity and low-capacity transport activity (Fig. 1). Since the AGT1 permease is a low-affinity (Km 20–30 mM) maltose transporter , in strain LCM001 maltose uptake from the medium was normally mediated by the above indicated high-affinity MALx1 (MAL21, MAL31 and MAL41) maltose transport activities (data not shown).
Sucrose utilization by yeast strains
Due to the increased interest in biomass-based industrial applications of S. cerevisiae, several approaches have been developed to engineer the metabolism of this microorganism towards a more aerobic or respiratory utilization of sugars. In one approach where the targets are key regulatory proteins, either overexpression of the transcriptional factor HAP4 (activating respiratory genes), or deletion of GCR1 and GCR2 (activators of glycolytic genes), HXK2 or REG1 (relieving glucose repression), results in yeast strains showing increased biomass production during glucose fermentation [52–56]. However, modifying these regulatory circuits may also have some undesired side-effects, including significant reductions in the specific growth rate of the cells, and consequently losses in biomass productivity, or even altered patterns of sugar (other than glucose) utilization [57–61]. Another logic approach would be to restrict sugar uptake from the medium, which in the case of glucose transport by S. cerevisiae was a huge challenge as it was required not only to delete the hole set of hexose transporters found in this yeast (compromising almost 20 different genes), but also required the expression of a mutant chimera between the low-affinity (HXT1) and high-affinity (HXT7) glucose transporters as the unique sugar permease at the plasma membrane [62–64]. Although this yeast strain will respire and produce higher biomass levels when grown on glucose, it still has some inconveniences, such as slow growth rates, fructose fermentation, and an inability to use other sugars (e.g. galactose, due to GAL2 deletion). Indeed, the modification of the glucose uptake system in E. coli [65, 66] also allowed the development of a bacteria with reduced overflow metabolism, and increased biomass production.
Our results show that when we engineer the mode of sucrose utilization by the yeast S. cerevisiae, allowing the direct uptake of the sugar by low-affinity and low-capacity transport systems, followed by its intracellular hydrolysis mediated by a maltase (α-glucosidase), the yeast cells grow efficiently on sucrose but produce significantly less ethanol since the cells are diverting more of this carbon source towards biomass production. Thus, higher biomass production can be attained with simple batch cultures in 20 g/L sucrose, avoiding some drawbacks of fed-batch cultures due to easier operational procedures, reduced equipment and process time needed for each production lot. Indeed, the more respiratory phenotype of strain LCM001 was clearly demonstrated by the occurrence of only one growth phase during batch cultivation with sucrose as the carbon and energy source, and the corresponding decrease (to the same levels of the parental strain 1403-7A) in the biomass yield when the respiratory inhibitor antimycin A was used.
Although S. cerevisiae has a strong tendency towards alcoholic fermentation of sugars, several reports have shown that in the case of some α-glucosides (e.g. maltotriose [67, 68] and trehalose [69–72]) which are transported by low-affinity and/or low-capacity uptake systems, the sugar may be completely respired by the yeast cell. However, these approaches have little practical application due to the high commercial prices of these sugar substrates. Nevertheless, all these studies, including the results shown in the present report, highlight the importance of the sugar transport step in the aerobic/fermentative dissimilation of sugars by yeast cells . It is also important to emphasize that the engineering strategy utilized in the present approach does not affect the utilization (and fermentation) of other sugars (e.g. glucose, fructose, maltose) commonly used by yeasts, and thus would not affect the downstream utilization of such strains in important industrial applications such as bread making, or production of distilled beverages. The high specific growth rates observed during batch growth of the engineered strain on sucrose, even when the sugar is consumed more slowly, is probably a consequence of the superior efficiency of this sugar (when compared to glucose) for binding to and stimulate the GPR1 sugar receptor in S. cerevisiae cells, an important signaling system that controls, among several other physiological aspects, yeast cell growth [10, 12]. It would thus be interesting to delete this GPR1 sugar receptor, and analyze the consequence of such deletion on the growth rate and metabolism of sucrose by S. cerevisiae cells. Finally, another way to further improve the biomass yield of yeasts grown at excess sucrose concentrations could be obtained by combining the properties of strain LCM001 with one or more of the above described strategies (e.g. HAP4 overexpression, and/or hxk2 deletion), or even by using the classical fed-batch mode of yeast cultivation. It remains to be seen whether combination of such approaches can further improve the biomass yield of S. cerevisiae at higher sucrose concentrations.
Higher cell densities during batch cultures on sucrose were achieved by using a S. cerevisiae strain engineered in the sucrose transport system. Deletion of the high-affinity sucrose transport system mediated by the AGT1 permease produced a yeast strain where sucrose was transported by low-affinity and low-capacity permeases. While up to 1.5 to 2-times more biomass, when compared with the parental strain, were obtained by the engineered yeast strain in simple batch cultivations using 20 g/L sucrose, the ability of the strains to efficiently ferment very-high sucrose concentrations (> 200 g/L) was unaffected. The yeast growth rate on rich medium containing 20 g/L sucrose was also unaffected, and thus the higher biomass yields were accomplished by preventing overflow metabolism and increasing respiration by the engineered strain, with the concomitant reduction in ethanol production. The simpler batch cultivation mode can be a viable option to more complicated traditional sucrose-limited fed-batch cultures. A thorough analysis of the physiological and transcriptional response of the engineered S. cerevisiae strain to very-high sucrose concentrations will help to better understand the regulatory mechanisms involved in sugar fermentation by yeasts, and could serve as a basis for engineering metabolic pathways to improve process performance of S. cerevisiae for biomass directed approaches using highly concentrated culture media.
Media and culture conditions
Cells were routinely grown on rich YP medium (10 g/L yeast extract and 20 g/L peptone), or synthetic medium (2 g/L yeast nitrogen base without aminoacids containing 75 mg/L L-tryptophane and 150 mg/L uracil) supplemented with different quantities of ammonium sulfate or peptone as nitrogen source, and 20 g/L glucose, sucrose or maltose as carbon source. The pH of each medium was adjusted to pH 5.0 with HCl, and media was either sterilized by filtration (synthetic medium), or autoclaved at 120°C for 20 min (rich YP medium). When required, 20 g/L agar, 3 mg/L antimycin A, or 200 mg/L geneticin (G-418) sulfate were added to the medium. Cells were grown aerobically at 28°C with shaking (160 rpm) in cotton plugged Erlenmeyer flasks filled to 1/5 of the volume with medium. The inoculum for growth assays was prepared by transferring aseptically a single colony from a plate into 5–10 mL of the selected medium containing 20 g/L glucose, and allowed to growth into stationary phase for 2 to 3 days before they were used to inoculate (by a 100 × dilution) new media containing the indicated carbon sources. Culture samples were harvested regularly, centrifuged (5,000 g, 1 min), and their supernatants used for the determination of sugars and ethanol. For batch fermentations yeasts were pregrown on YP-20 g/L sucrose until the exponential phase (~1 g of dry yeast/L), centrifuged (3,500 g, 3 min) and washed twice with cold water, and inoculated at a high cell density (10 g of dry yeast/L) into synthetic medium (4 g/L yeast nitrogen base without aminoacids and 10 g/L ammonium sulfate) or rich YP medium containing the indicated amounts of sucrose. Batch fermentations were incubated as described above for growth assays, and samples were collected regularly, centrifuged, and their supernatants analyzed as described below.
Saccharomyces cerevisiae strains and oligonucleotides used in this study
Relevant genotype or description:
Source or reference:
MAT a MAL4 C MGL3 suc - gal3 gal4 trp1 ura3
agt1 Δ::kanMX6 derivative of 1403-7A
MAT αAGT1 MAL12 mal13 MAL31 MAL32 mal33 gal2 mel flo1 flo8-1 hap1 SUC2
MAT a MAL2 ura3-52 his3-11,15 leu2-3,112 SUC2
PFGE, chromosome blotting and hybridization
Yeast chromosomes were prepared as previously described  from 1 ml of yeast cells pre-grown in YP-2% glucose medium and collected at the stationary phase of growth. Cells were washed with 10 mM Tris-HCl, pH 7.5, containing 50 mM EDTA, and resuspended in the 0.4 ml of 4 mM Tris-HCl, pH 7.5, containing 95 mM EDTA, 130 mg/L of Zymolyase 20T and 7 g/L of molten (42°C) low-melting-point agarose. After solidification in a mold (Pharmacia Biotech), the agarose blocks were immersed in 10 mM Tris-HCl, pH 7.5, containing 0.5 M EDTA, and incubated at 37°C for 8 hours. Following a subsequent incubation in 10 mM Tris-HCl, pH 9.5, containing 0.5 M EDTA, 1% N-lauroylsarcosine, and 2 g/L proteinase K at 50°C overnight, the blocks were washed in 10 mM Tris-HCl, pH 7.5, containing 50 mM EDTA, and stored at 4°C in the same buffer. Each low-melting-point agarose block was transferred to a 10 g/L agarose gel in 50 mM Tris-HCl, pH 8.3, containing 50 mM boric acid and 1 mM EDTA. Pulsed field gel electrophoresis (PFGE) was performed at 10°C using a Gene Navigator pulsed-field system (Pharmacia Biotech) for a total of 27 hours at 200 V. The pulse time was stepped from 70 seconds after 15 hours to 120 seconds for 12 hours. Following electrophoresis, the gel was stained with ethidium bromide and photographed. The chromosomes separated by PFGE were transferred to a nylon filter (Biodyne A, Gibco BRL) by capillary blotting , and labeling of DNA probes (see below), including the pre-hybridization, hybridization, stringency washes and chemiluminescent signal generation and detection was performed by using a AlkPhos kit (GE Healthcare/Amersham Biosciences) as recommended by the manufacturer. After hybridization, an autoradiography film (Hiperfilm™ ECL – Kodak) was exposed to the membrane for 2 to 3 h before it was developed. Images were obtained by scanning with an ImageScanner™ (Amersham Biosciences) and annotated with Microsoft PowerPoint. Probes corresponding to nucleotides +1 through +1848 on the AGT1 ORF, -73 through +1845 of the MAL31 gene, or +77 through + 1333 of the SUC2 locus were generated by PCR using primers AGT1-F and AGT1-R, MAL31-F and MAL31-R, and SUC2-F and SUC2-R (Table 1), respectively.
Sucrose was quantified using 50 U/mL of yeast β-D-fructosidase (Sigma) in 50 mM citrate-phosphate buffer, pH 4.5, followed by glucose determination. Glucose was measured by the glucose oxidase and peroxidase method using a commercial kit (BioDiagnostica-Laborclin). Maltose was determined spectrophotometrically at 540 nm with methylamine in 0.25 M NaOH as described previously . Ethanol was determined with alcohol oxidase and peroxidase as described previously [44, 50]. Cellular growth was followed by turbidity measurements at 570 nm after appropriate dilution, and yeast cell dry weight was determined as described elsewhere . Briefly, from 1 to 5 mL of fermentation broth was filtered through pre-weighed filters (0.45 μm mixed nitrocellulose and cellulose acetate filters), washed twice with 5 mL of distilled water, and after placing in a small (5 cm diameter) covered Petri dish, dried for 3 to 5 min in a microwave oven at maximum power (900 W) until constant weight. The sugar consumption rates were calculated using samples harvested from the logarithmic growth phase and/or in intervals during which maximal rates were attained. Mean values of dry weight in the specified time intervals were used in the rate calculations. Specific growth rate (μ, h-1) was determined as slope of a straight line between ln OD570 nm and time (h) during the initial (~12 h) exponential phase of growth. Biomass and ethanol yield coefficients (Yx/s and Ye/s, respectively) were obtained at the end of cell growth or ethanol production, taking into account the amount of sugar utilized. The kinetics of active H+-sucrose or H+-maltose symport were determined as previously described [40, 41] using a PHM84 research pH meter attached to a TT1 Servograph (Radiometer, Copenhagen). Initial rates of sugar-induced proton uptake were calculated from the slope of the initial part (< 10 s) of the curve obtained in the recorder, subtracting the basal rate of proton uptake observed before addition of 0.1–100 mM of the sugar. All determinations were done at least in duplicate, and assays were monitored so that no more than 5% of the substrate was depleted. All activities were expressed as nmol of substrate transported per mg dry cell weight per min.
This work was supported in part by grants from the Brazilian agencies CNPq (N° 502999/2004-7) and FAPESP (N° 04/10067-6). All authors were recipients of scholarships or research fellowships from CNPq or CAPES-Brazil. We thank M. Longtine (University of North Carolina) and A. Kruckeberg (University of Amsterdam) for providing plasmid pFA6a-kanMX6 and yeast strains, respectively.
- Bekatorou A, Psarianos C, Koutinas AA: Production of food grade yeasts. Food Technol Biotechnol. 2006, 44: 407-415.Google Scholar
- Hensing MC, Rouwenhorst RJ, Heijnen JJ, van Dijken JP, Pronk JT: Physiological and technological aspects of large-scale heterologous-protein production with yeasts. Antonie van Leeuw. 1995, 67: 261-279. 10.1007/BF00873690.View ArticleGoogle Scholar
- Chemler JA, Yan Y, Koffas MA: Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae. Microb Cell Fact. 2006, 23: 20-10.1186/1475-2859-5-20.View ArticleGoogle Scholar
- Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, Ho KA, Eachus RA, Ham TS, Kirby J, Chang MCY, Withers ST, Shiba Y, Sarpong R, Keasling JD: Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006, 440: 940-943. 10.1038/nature04640.View ArticleGoogle Scholar
- Galao RP, Scheller N, Alves-Rodrigues I, Breinig T, Meyerhans A, Díez J: Saccharomyces cerevisiae : a versatile eukaryotic system in virology. Microb Cell Fact. 2007, 6: 32-10.1186/1475-2859-6-32.View ArticleGoogle Scholar
- Sagt CM, Kleizen B, Verwaal R, de Jong MD, Muller WH, Smits A, Visser C, Boonstra J, Verkleij AJ, Verrips CT: Introduction of an N-glycosylation site increases secretion of heterologous proteins in yeasts. Appl Environ Microbiol. 2000, 66: 4940-4944. 10.1128/AEM.66.11.4940-4944.2000.View ArticleGoogle Scholar
- Sleep D, Finni C, Evans L: Enhanced protein expression through strain selection, gene disruption, improved vector design and co-expression of endogenous chaperones. Microb Cell Fact. 2006, 5: S29-10.1186/1475-2859-5-S1-S29.View ArticleGoogle Scholar
- Lagunas R: Energetic irrelevance of aerobiosis for S. cerevisiae growing on sugars. Mol Cel Biochem. 1979, 27: 139-146. 10.1007/BF00215362.View ArticleGoogle Scholar
- Verduyn C, Stouthamer AH, Scheffers WA, van Dijken JP: A theoretical evaluation of growth yields of yeasts. Antonie van Leeuw. 1991, 59: 49-63. 10.1007/BF00582119.View ArticleGoogle Scholar
- Rolland F, Winderickx J, Thevelein JM: Glucose-sensing and -signaling mechanisms in yeasts. FEMS Yeasts Res. 2002, 2: 183-201.View ArticleGoogle Scholar
- Bisson LF, Kunathigan V: On the trail of an elusive flux sensor. Res Microbiol. 2003, 154: 603-610. 10.1016/S0923-2508(03)00170-0.View ArticleGoogle Scholar
- Lemaire K, Van de Velde S, Van Dijck P, Thevelein JM: Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae. Mol Cell. 2004, 16: 293-299. 10.1016/j.molcel.2004.10.004.View ArticleGoogle Scholar
- Moreno F, Ahuatzi D, Riera A, Palomino CA, Herrero P: Glucose sensing through the Hxk2-dependent signalling pathway. Biochem Soc Trans. 2005, 33: 265-268. 10.1042/BST0330265.View ArticleGoogle Scholar
- Frick O, Wittmann C: Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis. Microb Cell Fact. 2005, 4: 30-10.1186/1475-2859-4-30.View ArticleGoogle Scholar
- Hisbollah MAH, Ramachandran KB: Comparative evaluation of various control schemes for fed-batch fermentation. Bioprocess Eng. 2002, 24: 309-318. 10.1007/s00449-001-0272-7.Google Scholar
- Cannizzaro C, Valentinotti S, von Stockar U: Control of yeast fed-batch process through regulation of extracellular ethanol concentration. Bioprocess Biosyst Eng. 2004, 26: 377-383. 10.1007/s00449-004-0384-y.View ArticleGoogle Scholar
- Finn B, Harvey LM, McNeil B: Near-infrared spectroscopic monitoring of biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch bioprocess. Yeast. 2006, 23: 507-517. 10.1002/yea.1371.View ArticleGoogle Scholar
- Riesenberg D, Guthke R: High-cell-density cultivation of microorganisms. Appl Microbiol Biotechnol. 1999, 51: 422-430. 10.1007/s002530051412.View ArticleGoogle Scholar
- Enfors SO, Jahic M, Rozkov A, Xu B, Hecker M, Jürgen B, Krüger E, Schweder T, Hamer G, O'Beirne D, Noisommit-Rizzi N, Reuss M, Boone L, Hewitt C, McFarlane C, Nienow A, Kovacs T, Trägardh C, Fuchs L, Revstedt J, Friberg PC, Hjertager B, Blomsten G, Skogman H, Hjort S, Hoeks F, Lin HY, Neubauer P, van der Lans R, Luyben K, Vrabel P, Manelius A: Physiological responses to mixing in large scale bioreactors. J Biotechnol. 2001, 85: 175-185. 10.1016/S0168-1656(00)00365-5.View ArticleGoogle Scholar
- Berg C: World fuel ethanol – analysis and outlook. [http://www.distill.com/World-Fuel-Ethanol-A&O-2004.html]
- Peters D: Carbohydrates for fermentation. Biotechnol J. 2006, 1: 806-814. 10.1002/biot.200600041.View ArticleGoogle Scholar
- Naumov GI, Naumova ES, Sancho ED, Korhola MP: Polymeric SUC genes in natural populations of Saccharomyces cerevisiae. FEMS Microbiol Lett. 1996, 135: 31-35. 10.1111/j.1574-6968.1996.tb07962.x.View ArticleGoogle Scholar
- Korshunova IV, Naumova ES, Naumov GI: Comparative molecular genetic analysis of β-fructosidases of yeasts Saccharomyces. Mol Biol. 2005, 39: 413-419. 10.1007/s11008-005-0051-7.View ArticleGoogle Scholar
- Carlson M, Botstein D: Two differentially regulated mRNAs with different 5' ends encode secreted and intracellular forms of yeast invertase. Cell. 1982, 28: 145-154. 10.1016/0092-8674(82)90384-1.View ArticleGoogle Scholar
- Bokossa IP, Krastanov AI, Rochkova Z, Angelov A: Biosynthesis of invertase by Saccharomyces cerevisiae with sugarcane molasses as substrate. World J Microbio Biotech. 1993, 9: 662-663. 10.1007/BF00369576.View ArticleGoogle Scholar
- Ozcan S, Vallier LG, Flick JS, Carlson M, Johnston M: Expression of the SUC2 gene of Saccharomyces cerevisiae is induced by low levels of glucose. Yeast. 1997, 13: 127-137. 10.1002/(SICI)1097-0061(199702)13:2<127::AID-YEA68>3.0.CO;2-#.View ArticleGoogle Scholar
- Herwing C, Doerries C, Marison I, von Stockar U: Quantitative analysis of the regulation scheme of invertase expression in Saccharomyces cerevisiae. Biotechnol Bioeng. 2001, 76: 247-258. 10.1002/bit.10004.View ArticleGoogle Scholar
- Belinchón MM, Gancedo JM: Different signalling pathways mediate glucose induction of SUC2, HXT1 and pyruvate decarboxylase in yeast. FEMS Yeast Research. 2007, 7: 40-47.View ArticleGoogle Scholar
- Belinchón MM, Gancedo JM: Glucose controls multiple processes in Saccharomyces cerevisiae through diverse combinations of signaling pathways. FEMS Yeast Research. 2007, 7: 808-818. 10.1111/j.1567-1364.2007.00236.x.View ArticleGoogle Scholar
- Takeshige K, Ouchi K: Effects of yeast invertase on ethanol production in molasses. J Ferment Bioeng. 1995, 79: 513-515. 10.1016/0922-338X(95)91274-9.View ArticleGoogle Scholar
- Myers DK, Lawlor DTM, Attfield PV: Influence of invertase activity and glycerol synthesis and retention on fermentation of media with a high sugar concentration by Saccharomyces cerevisiae. Appl Environ Microbiol. 1997, 63: 145-150.Google Scholar
- Echegaray OF, Carvalho JCM, Fernandes ANR, Sato S, Aquarone E, Vitolo M: Fed-batch culture of Saccharomyces cerevisiae in sugar-cane blackstrap molasses: invertase activity of intact cells in ethanol fermentation. Biomass Bioenergy. 2000, 19: 39-50. 10.1016/S0961-9534(00)00022-2.View ArticleGoogle Scholar
- Greig D, Travisano M: The Prisoner's Dilemma and polymorphism in yeast SUC genes. Proc Biol Sci. 2004, 271: S25-S26. 10.1098/rsbl.2003.0083.View ArticleGoogle Scholar
- Berthels NJ, Otero RRC, Bauer FF, Thevelein JM, Pretorius IS: Discrepancy in glucose and fructose utilization during fermentation by Saccharomyces cerevisiae wine yeast. FEMS Yeast Res. 2004, 4: 683-689. 10.1016/j.femsyr.2004.02.005.View ArticleGoogle Scholar
- Orlowski JH, Barford JP: Direct uptake of sucrose by Saccharomyces cerevisiae in batch and continuous culture. J Gen Appl Microbiol. 1991, 37: 215-218. 10.2323/jgam.37.215.View ArticleGoogle Scholar
- Barford JP, Phillips PJ, Orlowski JH: A new model of uptake of multiple sugars by S. cerevisiae. Bioproc Eng. 1992, 7: 303-307. 10.1007/BF00705159.View ArticleGoogle Scholar
- Mwesigye PK, Barford JP: Mechanism of sucrose utilization by Saccharomyces cerevisiae. J Gen Appl Microbiol. 1996, 42: 209-306. 10.2323/jgam.42.297.View ArticleGoogle Scholar
- Mwesigye PK, Barford JP: Transport of sucrose by Saccharomyces cerevisiae. J Ferment Bioeng. 1994, 77: 687-690. 10.1016/0922-338X(94)90154-6.View ArticleGoogle Scholar
- Stambuk BU, da Silva MA, Panek AD, de Araújo PS: Active α-glucoside transport in Saccharomyces cerevisiae. FEMS Microbiol Letters. 1999, 170: 105-110.Google Scholar
- Stambuk BU, Batista AS, de Araújo PS: Kinetics of active sucrose transport by Saccharomyces cerevisiae. J Biosci Bioeng. 2000, 89: 212-214. 10.1016/S1389-1723(00)88742-3.View ArticleGoogle Scholar
- Stambuk BU, de Araújo PS: Kinetics of active α-glucoside transport by Saccharomyces cerevisiae. FEMS Yeast Res. 2001, 1: 73-78.Google Scholar
- Khan NA, Zimmermann FK, Eaton NR: Genetic and biochemical evidence of sucrose fermentation by maltase in yeast. J Mol Gen Genet. 1973, 123: 43-50. 10.1007/BF00282987.View ArticleGoogle Scholar
- Batista AS, Miletti LC, Stambuk BU: Sucrose fermentation by Saccharomyces cerevisiae lacking hexose transport. J Mol Microbiol Biotechnol. 2004, 8: 26-33. 10.1159/000082078.View ArticleGoogle Scholar
- Badotti F, Batista AS, Stambuk BU: Sucrose active transport and fermentation by Saccharomyces cerevisiae. Braz Arch Biol Technol. 2006, 49: s115-s123.View ArticleGoogle Scholar
- Oda Y, Ouchi K: Construction of a sucrose-fermenting baker's yeast incapable of hydrolysing fructooligosaccharides. Enzyme Microb Technol. 1991, 13: 495-498. 10.1016/0141-0229(91)90008-X.View ArticleGoogle Scholar
- Han EK, Cotty F, Sottas C, Jiang H, Michels CA: Characterization of AGT1 encoding a general α-glucoside transporter from Saccharomyces. Mol Microbiol. 1995, 17: 1093-1107. 10.1111/j.1365-2958.1995.mmi_17061093.x.View ArticleGoogle Scholar
- Michels CA, Needleman RB: The dispersed, repeated family of MAL loci in Saccharomyces spp. J Bacteriol. 1984, 157: 949-952.Google Scholar
- Da Cruz SH, Cilli EM, Ernandes JR: Structural complexity of the nitrogen source and influence on yeast growth and fermentation. J Inst Brew. 2002, 108: 54-61.View ArticleGoogle Scholar
- Shimizu H, Mizuno S, Hiroshima T, Shioya S: Effect of carbon and nitrogen additions on consumption activity of apparent extract of yeast cells in a brewing process. J Am Soc Brew Chem. 2002, 60: 163-169.Google Scholar
- Alves SL, Herberts RA, Hollatz C, Miletti LC, Stambuk BU: Maltose and maltotriose active transport and fermentation by Saccharomyces cerevisiae. J Am Soc Brew Chem. 2007, 65: 99-104.Google Scholar
- Lewis JG, Northcott CJ, Learmonth RP, Attfield PV, Watson K: The need for consistent nomenclature and assessment of growth phases in diauxic cultures of Saccharomyces cerevisiae. J Gen Microbiol. 1993, 139: 835-839.View ArticleGoogle Scholar
- Blom I, De Matos JMT, Grivell LA: Redirection of the respirofermentative distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p. Appl Environ Microbiol. 2000, 66: 1970-1973. 10.1128/AEM.66.5.1970-1973.2000.View ArticleGoogle Scholar
- Van Maris AJA, Bakker BM, Brandt M, Boorsma A, De Mattos MJT, Grivell LA, Pronk JT, Blom J: Modulating the distribution of fluxes among respiration and fermentation by overexpression of HAP4 in Saccharomyces cerevisiae. FEMS Yeast Res. 2001, 1: 139-149.View ArticleGoogle Scholar
- Diderich JA, Raamsdonk LM, Kruckeberg AL, Berden JA, van Dam K: Physiological properties of Saccharomyces cerevisiae from which hexokinase II has been deleted. Appl Environ Microbiol. 2001, 67: 1587-1593. 10.1128/AEM.67.4.1587-1593.2001.View ArticleGoogle Scholar
- Diderich JA, Raamsdonk LM, Kuiper A, Kruckeberg AL, Berden JA, de Mattos MJT, van Dam K: Effects of a hexokinase II deletion on the dynamics of glycolysis in continuous cultures of Saccharomyces cerevisiae. FEMS Yeast Res. 2002, 1482: 1-8.Google Scholar
- Sasaki H, Uemura H: Influence of low glycolytic activities in gcr1 and gcr2 mutants on the expression of other metabolic pathway genes in Saccharomyces cerevisiae. Yeast. 2005, 22: 111-27. 10.1002/yea.1198.View ArticleGoogle Scholar
- Entian KD, Loureiro-Dias MC: Misregulation of maltose uptake in a glucose repression defective mutant of Saccharomyces cerevisiae leads to glucose poisoning. J Gen Microbiol. 1990, 136: 855-860.View ArticleGoogle Scholar
- Lascaris R, Bussemaker HJ, Boorsma A, Piper M, van der Spek H, Grivell L, Blom J: Hap4p overexpression in glucose-grown Saccharomyces cerevisiae induces cells to enter a novel metabolic state. Genome Biol. 2002, 4: 1-10. 10.1186/gb-2002-4-1-r3.View ArticleGoogle Scholar
- Lascari R, Piwowarski J, Van der Spek H, de Mattos JT, Grivell L, Blom J: Overexpression of HAP4 in glucose-derepressed yeast cells reveals respiratory control of glucose-regulated genes. Microbiology. 2004, 150: 929-934. 10.1099/mic.0.26742-0.View ArticleGoogle Scholar
- Herwig C, Chetreanu F, Niederberger P, Marison I, Stockar UV: Quantitative analysis of the impact of HXK2 and REG1 deletion in Saccharomyces cerevisiae on invertase expression and respiration. Enzyme Microbial Technol. 2002, 31: 505-515. 10.1016/S0141-0229(02)00145-X.View ArticleGoogle Scholar
- Barbara KE, Haley TM, Willis KA, Santangelo GM: The transcription factor Gcr1 stimulates cell growth by participating in nutrient-responsive gene expression on a global level. Mol Genet Genomics. 2007, 277: 171-88. 10.1007/s00438-006-0182-0.View ArticleGoogle Scholar
- Otterstedt K, Larsson C, Bill RM, Stahlberg A, Boles E, Hohmann S, Gustafsson L: Switching the mode of metabolism in the yeast Saccharomyces cerevisiae. EMBO Rep. 2004, 5: 532-537. 10.1038/sj.embor.7400132.View ArticleGoogle Scholar
- Elbing K, Larsson C, Bill RM, Albers E, Snoep JL, Boles E, Hohmann S, Gustafsson L: Role of hexose transport in control of glicolytic flux in Saccharomyces cerevisiae. Appl Environ Microbiol. 2004, 70: 5323-5330. 10.1128/AEM.70.9.5323-5330.2004.View ArticleGoogle Scholar
- Henricsson C, de Jesus Ferreira MC, Hedfalk K, Elbing K, Larsson C, Bill RM, Norbeck J, Hohmann S, Gustafsson L: Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrations. Appl Envir Microbiol. 2005, 71: 6185-6192. 10.1128/AEM.71.10.6185-6192.2005.View ArticleGoogle Scholar
- Gosset G: Improvement of Escherichia coli production strains by modification of the phosphoenolpyruvate:sugar phosphotransferase system. Microb Cell Factories. 2005, 4: 14-10.1186/1475-2859-4-14.View ArticleGoogle Scholar
- De Anda R, Lara AR, Hernández V, Hernández-Montalvo V, Gosset G, Bolívar F, Ramírez OT: Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate. Metab Eng. 2006, 8: 281-90. 10.1016/j.ymben.2006.01.002.View ArticleGoogle Scholar
- Zastrow CR, Mattos MA, Hollatz C, Stambuk BU: Maltotriose metabolism by Saccharomyces cerevisiae. Biotechnol Lett. 2000, 22: 455-459. 10.1023/A:1005691031880.View ArticleGoogle Scholar
- Zastrow CR, Hollatz C, de Araujo PS, Stambuk BU: Maltotriose fermentation by Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2001, 27: 34-38. 10.1038/sj.jim.7000158.View ArticleGoogle Scholar
- Malluta EF, Decker P, Stambuk BU: The Kluyver effect for trehalose in Saccharomyces cerevisiae. J Basic Microbiol. 2000, 40: 199-205. 10.1002/1521-4028(200007)40:3<199::AID-JOBM199>3.0.CO;2-E.View ArticleGoogle Scholar
- Jules M, Guillou V, Francois J, Parrou J: Two distinct pathways for trehalose assimilation in the yeast Saccharomyces cerevisiae. Appl Environ Microbiol. 2004, 70: 2771-2778. 10.1128/AEM.70.5.2771-2778.2004.View ArticleGoogle Scholar
- Jules M, Francois J, Parrou J: Autonomous oscillations in Saccharomyces cerevisiae during batch cultures on trehalose. FEBS J. 2005, 272: 1490-500. 10.1111/j.1742-4658.2005.04588.x.View ArticleGoogle Scholar
- Mouret JR, Jacobsen JN, Guillouet SE: Kinetic analysis of a trehalase-overexpressing strain grown on trehalose: a new tool for respire-fermentative transition studies in Saccharomyces cerevisiae. Lett Appl Microbiol. 2006, 42: 363-368. 10.1111/j.1472-765X.2006.01869.x.View ArticleGoogle Scholar
- Goffrini P, Ferrero I, Donnini C: Respiration-dependent utilization of sugars in yeasts: a determinant role for sugar transporters. J Bacteriol. 2002, 184: 427-432. 10.1128/JB.184.2.427-432.2002.View ArticleGoogle Scholar
- Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Short Protocols in Molecular Biology. 1995, John Wiley & Sons: New York, N.Y, 3Google Scholar
- Petracek ME, Longtine MS: PCR-based engineering of yeast genome. Methods in Enzymol. 2002, 350: 445-469.View ArticleGoogle Scholar
- Guerring SL, Connelly C, Hieter P: Positional mapping of genes by chromosome blotting and chromosome fragmentation. Methods Enzymol. 1991, 194: 57-77.View ArticleGoogle Scholar
- Rice JF, Sullivan TR, Helbert JR: A rapid method for the determination of yeast dry weight concentration. J Am Soc Brew Chem. 1980, 38: 142-145.Google Scholar
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