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Table 1 Saccharomyces cerevisiae strains and oligonucleotides used in this study

From: Switching the mode of sucrose utilization by Saccharomyces cerevisiae

  Relevant genotype or description: Source or reference:
Yeast Strains   
1403-7A MAT a MAL4 C MGL3 suc - gal3 gal4 trp1 ura3 ATTC 208023
LCM001 agt1 Δ::kanMX6 derivative of 1403-7A This study
S288C MAT αAGT1 MAL12 mal13 MAL31 MAL32 mal33 gal2 mel flo1 flo8-1 hap1 SUC2 (39)
MC966A MAT a MAL2 ura3-52 his3-11,15 leu2-3,112 SUC2 (43)
Primers   
AGT1-pFA6-F1 AAGCAAGAAGAAGGCTGCCTCAAAAAATGAGGATAAAAACATTTCTGAGCGGATCCCCGGGTTAATTAA Invitrogen
AGT1-pFA6-R1 AAAGGGATTCCTTATTTCTTCCAAAAAAAAAAAAACAACCCTTTTACTTAGAATTCGAGCTCGTTTAAAC "
AGT1-F AGGAGCTCATGAAAAATATCATTTCATTGG Gibco BRL
AGT1-R TTGGATCCACATTTATCAGCT GC "
MALx1-F CCATACTTGTTGTGAGTGG "
MALx1-R TCATTTGTTCACAACAGATG Invitrogen
SUC2-F GCGATAGACCTTTGGTCCAC Gibco BRL
SUC2-R GGACCGTGGTAACTCTAAGG "
V-AGT1-F GAATTTTCGGGTTGGTG "
V-AGT1-R TTGGATCCACATTTATCAGCTGC "
V-kanr-R GGAATCGAATGCAACCGG "