Bacillus megaterium as a recombinant protein production host
© Yang et al; licensee BioMed Central Ltd. 2006
- Published: 10 October 2006
- Shake Flask Cultivation
- Ethylene Terephthalate
- Bacillus Megaterium
- Xylose Isomerase
- High Cell Density Cultivation
The gram positive soil bacterium Bacillus megaterium is well known for its industrial utilization for production of various extracellular enzymes as amylases. Recently production and secretion of recombinant proteins in B. megaterium was also studied [1, 2]. In this contribution a homologous model protein (penicillin G amidase (PGA) from B. megaterium ATCC14945) and a heterologous protein (a hydrolase from Thermobifida fusca DSM43793 (TFH)) were used to further investigate and improve the system. Penicillin amidase is applied in the synthesis of semisynthetic penicillins. TFH is able to degrade specific polyesters such as poly (ethylene terephthalate) (PET) or poly (butylene terephthalate) (PBT), which are hither to regarded as 'non-biodegradable' plastics .
In this new host system both proteins were secreted directly into supernatant and the good productivity was obtained from fermentation.
Open AccessThis article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Malten M, Hollmann R, Deckwer WD, Jahn D: Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium. Biotechnol Bioeng. 2005, 89: 206-218. 10.1002/bit.20341.View ArticleGoogle Scholar
- Malten M, Biedendieck R, Gamer M, Drews AC, Stammen S, Buchholz K, Dijkhuizen L, Jahn D: A Bacillus megaterium Plasmid System for the Production, Export, and One-Step Purification of Affinity-Tagged Heterologous Levansucrase from Growth Medium. Appl Environ Microbiol. 2006, 72: 1677-1679. 10.1128/AEM.72.2.1677-1679.2006.View ArticleGoogle Scholar
- Müller RJ, Schrader H, Profe J, Dresler K, Deckwer WD: Enzymatic Degradation of Poly(ethylene terephthalate): Rapid Hydrolyse using a Hydrolase from T. fusca. Macromolecular Rapid Communications. 2005, 26: 1400-1405. 10.1002/marc.200500410. 10.1002/marc.200500410.View ArticleGoogle Scholar
- Grote A, Hiller K, Scheer M, Munch R, Nortemann B, Hempel DC, Jahn D: JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Res. 2005, 33: W526-W531. 10.1093/nar/gki376.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd.