- Open Access
Identification and physiological characterization of phosphatidic acid phosphatase enzymes involved in triacylglycerol biosynthesis in Streptomyces coelicolor
© Comba et al; licensee BioMed Central Ltd. 2012
- Received: 23 August 2012
- Accepted: 20 January 2013
- Published: 29 January 2013
Phosphatidic acid phosphatase (PAP, EC 126.96.36.199) catalyzes the dephosphorylation of phosphatidate yielding diacylglycerol (DAG), the lipid precursor for triacylglycerol (TAG) biosynthesis. Despite the importance of PAP activity in TAG producing bacteria, studies to establish its role in lipid metabolism have been so far restricted only to eukaryotes. Considering the increasing interest of bacterial TAG as a potential source of raw material for biofuel production, we have focused our studies on the identification and physiological characterization of the putative PAP present in the TAG producing bacterium Streptomyces coelicolor.
We have identified two S. coelicolor genes, named lppα (SCO1102) and lppβ (SCO1753), encoding for functional PAP proteins. Both enzymes mediate, at least in part, the formation of DAG for neutral lipid biosynthesis. Heterologous expression of lppα and lppβ genes in E. coli resulted in enhanced PAP activity in the membrane fractions of the recombinant strains and concomitantly in higher levels of DAG. In addition, the expression of these genes in yeast complemented the temperature-sensitive growth phenotype of the PAP deficient strain GHY58 (dpp1lpp1pah1). In S. coelicolor, disruption of either lppα or lppβ had no effect on TAG accumulation; however, the simultaneous mutation of both genes provoked a drastic reduction in de novo TAG biosynthesis as well as in total TAG content. Consistently, overexpression of Lppα and Lppβ in the wild type strain of S. coelicolor led to a significant increase in TAG production.
The present study describes the identification of PAP enzymes in bacteria and provides further insights on the genetic basis for prokaryotic oiliness. Furthermore, this finding completes the whole set of enzymes required for de novo TAG biosynthesis pathway in S. coelicolor. Remarkably, the overexpression of these PAPs in Streptomyces bacteria contributes to a higher productivity of this single cell oil. Altogether, these results provide new elements and tools for future cell engineering for next-generation biofuels production.
- Oleaginous bacteria
- Lipid metabolism
PAP enzymes (EC 188.8.131.52) have been identified and characterized in various eukaryotes. In yeast cells, PAP activity plays a central role in both lipid metabolism and intracellular signaling mechanisms[15, 16]. Two distinct family of PAPs, referred to as PAP1 and PAP2, have been described[17–19]. The enzymes belonging to the PAP1 family show a Mg2+-dependent PAP activity, utilize PA as a unique substrate and localize in the soluble fraction of the cell[20–22]. In contrast, the PAP2 enzymes utilize an array of different substrates such as PA, LPA, sphingosine-1- phosphate and diacylglycerol pyrophosphate (DGPP) among others. This family, currently known as lipid phosphate phosphatases LPPs, do not require Mg2+ for activity and are integral membrane proteins.
The first member of the PAP1 family of enzymes (Pah1) has been purified and characterized from the membrane and cytosolic fractions of yeast. The analysis of mutants lacking the pah1 gene has provided evidences that this enzyme generates the DAG used for lipid synthesis. Cells containing a pah1 mutation accumulate PA and have reduced amounts of DAG and its acylated derivative TAG. The genes encoding for the PAP1 family of enzymes are highly conserved among eukaryotic species, but they do not possess any homologues in bacterial genomes.
On the other hand, the main PAP2 enzymes are encoded in yeast by the dpp1 and lpp1 genes, with the former being the principal contributor of this activity. The dpp1 and lpp1 gene products are integral membrane proteins with six transmembrane spanning regions and are localized in the vacuole[28, 29] and Golgi compartments of the cell, respectively. The Dpp1 enzyme shows a preference for DGPP as a substrate, whereas the Lpp1 enzyme has similar substrate specificity for both PA and DGPP. Overall, these enzymes belong to the PAP2 superfamily (pfam 01569), which includes more than 600 eukaryotic and prokaryotic proteins. Within the members of this group, PgpB of E. coli is the only enzyme known to display PAP activity. Originally, pgpB was identified in a screen designed to isolate cells defective in phosphatidylglycerol phosphate (PGP) phosphatase activity. However, further analyses suggested that PgpB had a broad substrate spectrum, as demonstrated by its in vitro phosphatase activities towards PGP, PA, LPA, DGPP and undecaprenyl pyrophosphate (C55-PP)[33–36]. Touzé et al. reported that PgpB prefers pyrophosphate lipids as substrates and indicated that this enzyme is involved in the C55-P metabolism.
Interestingly within this superfamily, Nakamura et al., identified and characterized plastidic PAP2 enzymes in Arabidopsis thaliana (LPPsβ, γ, δ, ε1 and ε2) and its homologue in Synechocystis sp. PCC6803 (synLPP). The author suggested that theses enzymes belong to a prokaryotic subfamily of PAP2 and could be involved in providing DAG precursors for monogalactosyl and digalactosyl diacylglycerol synthesis.
Remarkably, despite the functional relevance of these proteins in lipid metabolism of oleaginous bacteria, no studies were conducted towards the identification and characterization of PAPs in this group of bacteria. In an effort to unravel the biochemical properties and physiological significance of these proteins in S. coelicolor, we carried out a comprehensive bioinformatic analyses to identify and further characterize the PAP enzyme(s) of this microorganism. In this study we present the genetic and biochemical characterization of two Streptomyces PAPs, specifically demonstrating that SCO1102 (lppα) and SCO1753 (lppβ) encode PAP enzymes catalyzing the formation of DAG from PA and that both proteins are essential for achieving wild type levels of TAG in this microorganism.
Identification of putative PAPs in S. coelicolor
Phylogenetic analysis using curated sequences of LPPs from different organisms showed that S. coelicolor LPPs (scoLPPs) are evolutionary associated with synLPP, in the subgroup of prokaryotic LPPs (Figure 2). Non-plastidic LPPs of A. thaliana (atLPPα1, 2, 3) are clustered together with LPPs from human, murine and S. cerevisiae in a clade merely composed by eukaryotes LPPs (Figure 2). In agreement with Nakamura et al., our studies also show that bacterial LPPs group closer to the clade of “plastidic LPPs with prokaryote origin” (atLPPδ, ε1 and ε2) than to the eukaryotic one.
Additional bioinformatic analysis of these sequences revealed that scoLppα (Sco1102) orthologues are conserved in all the available Streptomyces genomes, as well as in other species of actinomycetes belonging to the genus Mycobacterium, Rhodococcus and Nocardia, among others. The presence of scoLppβ (Sco1753) homologous sequences is however limited to the Streptomyces genus, being present in all the species sequenced up to date. Finally, scoLppγ (Sco6355) coding sequence is conserved only in S. coelicolor and in S. avermitilis. This gene is part of a five member operon (SCO6353-SCO6357) that also encodes for a putative sensor histidine kinase and a response regulator. The genome organization of scoLppγ and the fact that this operon is absent in other actinomycetes, suggest a specific role of this protein in S. coelicolor and S. avermitilis metabolism.
Overall, orthologues sequences to scoLppα and scoLppβ are widely distributed among oleaginous bacteria, merely actinomycetes; thus these two proteins can be considered as the best PAP candidates.
Heterologous expression of S. coelicolor Lppα, Lppβ and Lppγ in E. coli
Phosphatidic acid phosphatase activity in membranes of E. coli expressing scoLPPs
Specific PAP activitya
0.9 ± 0.2
2.51 ± 0.06
4.5 ± 0.1
1.02 ± 0.08
Lppα and Lppβ complement the temperature sensitive phenotype of dpp1lpp1pah1- deficient yeast cells
Since the heterologous expression of Lppγ did not show a clear effect in lipid metabolism, neither in E. coli nor in S. cerevisiae, we continued the characterization of the other two S. coelicolor PAP candidates.
Lppα and Lppβ are involved in TAG biosynthesis in S. coelicolor
Strains and plasmids
Strain or plasmid
Parental strain, SCP1- SCP2-
SCO1102::Tn5062, derivative of M145; AmR
SCO1753::Tn5062, derivative of M145; AmR
SCO1102::Tn5062 SCO1753::Tn5066, derivative of SC_1102; AmR HygR
M145 attB ΦBT1 ::pTR285; KmR
M145 attB ΦBT1 ::p285-LPPα; KmR
M145 attBΦBT 1::p285-LPPβ; KmR
SC_1153 attB ΦBT1 ::pRT802-LPPα; AmR HygR KmR
SC_1153 attBΦBT 1::pRT802-LPPβ; AmR HygR KmR
E. coli K12 F - lacU169 (Φ80lacZΔM15) endA1 recA1 hsdR17 deoR supE44 thi-1 l2 gyrA96 relA1
F – ompT gal dcm lon hsdS B (r B - m B - ) λ(DE3)
F – ompT gal dcm lon hsdS B (r B - m B - ) λ(DE3)
supE44 hsdS20 ara - 14 proA2 lacY galK2 rpsL20 xyl - 5 mtl - 1 dam - dcm - hsdM - ; CmR
MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1
W303-1A dpp1 _::TRP1/Kan R lpp1 _::HIS3/Kan R pah1 _::URA3
Vector for expression of N terminal His-tagged proteins under the strong T7 promoter; KmR
Vector for recombinant protein expression under the control of the P BAD promoter; CmR
Vector used for cloning of blunt PCR products; KmR
RK2 derivative with defective oriT; KmR
RK2 derivative with defective oriT; ApR
B. Julien (Personal communication)
Plasmid carrying a copy of Tn5066; HygR
P. Dyson, (Personal communication)
Intregrative vector based on ΦBT1 phage integrase; KmR
Multicopy Yeast/E. coli expression vector with GPD promoter and LEU2 marker; ApR
pRT802 derivative carrying the P ermE* promoter with no gene under its control; KmR
pBAD33 carrying the SCO0958His gene under the control of P BAD promoter; CmR
pBAD33 carrying the SCO1102His gene under the control of P BAD promoter; CmR
pBAD33 carrying the SCO1753His gene under the control of P BAD promoter; CmR
pBAD33 carrying the SCO6355His gene under the control of P BAD promoter; CmR
p425-GPD carrying the SCO1102His gene under the control of GPD promoter; ApR, LEU2
p425-GPD carrying the SCO1753His gene under the control of GPD promoter; ApR, LEU2
p425-GPD carrying the SCO6355His gene under the control of GPD promoter; ApR, LEU2
pET28 carrying the SCO1102His gene under the control of T7 promoter; KmR
pET28 carrying the SCO1753His gene under the control of T7 promoter; KmR
pTR285 carrying the SCO1102His gene under the control of P ermE* promoter; KmR
pTR285 carrying the SCO1753His gene under the control of P ermE* promoter; KmR
pRT802 carrying the SCO1102His gene under the control of its own promoter; KmR
pRT802 carrying the SCO1753His gene under the control of its own promoter; KmR
These experiments are in agreement with the in vivo and in vitro studies mentioned before, and indicate that both Lppα and Lppβ are functional PAPs involved in TAG biosynthesis in S. coelicolor.
Co-expression of S. coelicolor PAP and DGAT enzymes leads to TAG biosynthesis in E. coli
In this work we described the identification and physiological characterization of the first phosphatidic phosphatase enzymes involved in triacylglycerol biosynthesis in bacteria.
By means of a comprehensive bioinformatic analysis of the S. coelicolor genome, we were able to identify Lppα and Lppβ as the best PAP candidates of this microorganism. They were both predicted to be integral membrane proteins belonging to the PAP2 family of enzymes also known as LPPs (pfam01569; Figure 2). Heterologous expression of S. coelicolor PAPs (scoLPPs) in E. coli increased the intracellular levels of DAG up to 6-fold and enhanced PAP activity in their membrane fractions up to 5-fold when compared with the control strain (Figure 3B, Table 1). Furthermore, when expressed in yeast, scoLPPs were able to complement the temperature sensitive phenotype of a dpp1 lpp1 pah1 deficient mutant (Figure 4), and to raise the intracellular concentration of DAG.
To unravel the physiological role of Lppα and Lppβ in S. coelicolor, we constructed the corresponding simple and double mutant strains. Disruption of either lppα or lppβ caused no alteration in the intracellular levels of TAG. However, inactivation of both genes, lppα and lppβ, led to a clear reduction of the membrane associated PAP activity, as well as a reduction of TAG accumulation (Figure 5A). Further, overexpression of these genes in a wild type background provoked an increase of 15.7% and 29.9% in the content of TAG for Lppα and Lppβ, respectively (Figure 5B). These observations support an active role of these two PAP enzymes in the TAG biosynthesis pathway of S. coelicolor.
Finally, co-expression of the S. coelicolor DGAT enzyme Sco0958 together with Lppα or Lppβ in E. coli reconstituted the complete pathway for de novo TAG biosynthesis in a heterologous host (Figure 6).
The identification of Lppα and Lppβ completes the minimal set of enzymes, together with the three DGATs previously characterized, required for de novo TAG biosynthesis in S. coelicolor. Remarkably, the overexpression of these PAPs in Streptomyces bacteria contributes to a higher productivity of this single cell oil.
Overall, these results provide new elements and tools for future cell engineering towards achieving sustainable and cost-effective single cell oil production in oleaginous bacteria. Furthermore, the successful reconstruction of the specific TAG biosynthesis pathway in a heterologous host with good fermentation properties such as E. coli broadens the bacterial platforms for next-generation biofuels production.
Strains, media and growth conditions
The strains and plasmids used in this study are described in Table 2. E. coli strains were grown either on solid or in liquid Luria-Bertani (LB) medium at 37°C and supplemented when needed with the following antibiotics: 100 μg ml-1 ampicillin (Ap), 50 μg ml-1 kanamycin (Km), 20 μg ml-1 chloramphenicol (Cm), 100 μg ml-1 hygromycin (Hyg) or 50 μg ml-1 apramycin (Am). Yeast cells were grown in YPD medium (1% yeast extract, 2% peptone, 2% glucose) or in synthetic complete (SC) medium containing 2% glucose at 30°C. For selection of yeast cells bearing plasmids, appropriate amino acids were omitted from SC medium. Streptomyces strains were grown at 30°C in liquid media R5 or SMM and on solid media MS, R5, or in SMMS containing 1% w/v glucose (SMMS-glucose). The antibiotics Am, Hyg and Km were added at final concentrations of 50 μg ml-1 to solid medium respectively.
Construction of lppα and lppβ mutants and the lppα lppβ double mutant strain of S. coelicolor
In order to generate the double mutant strain, the Am resistance marker of cosmid I34.1.D02 was replaced by the Hyg resistance cassette of pQM5066 (P. Dyson, personal communication). Conjugal transfer of cosmid I34.1.D02 HygR to SC_1102 yielded AmR HygR KmS exconjugants. Three independent clones were picked and checked by PCR with the primer pairs described above for the mutations in SCO1102 and SCO1753.
Cloning of lppα, lppβ and lppγ genes
lppα (SCO1102), lppβ (SCO1753) and lppγ (SCO6355) were amplified from S. coelicolor M145 genomic DNA by PCR with primers SCO1102_F/R, SCO1753_F/R and SCO6355_F/R, respectively. The resulting PCR products were cloned in pCR®-BluntII-TOPO vector and checked by DNA sequencing. The DNA fragments containing lppα, lppβ and lppγ genes were cloned as NdeI/EcoRI digests in pET28a, to yield plasmids p28-LPPα, p28-LPPβ and p28-LPPγ. pET28 derivative plasmids containing each of the putative PAP sequences were digested with XbaI/HindIII and the restriction fragments were cloned in the same sites of pBAD33, yielding pBAD-LPPα, pBAD-LPPβ, pBAD-LPPγ. In order to express PAP candidate genes in S. cerevisiae, the XbaI/HindIII digestion fragments from the pET28 derivatives (p28-LPPα, p28-LPPβ, p28-LPPγ) were cloned in the SpeI/HindIII sites of p425GPD vector, yielding p425-LPPα, p425-LPPβ and p425-LPPγ. To achieve overexpression of lppα and lppβ genes in S. coelicolor, the pET28 derivative plasmids containing these genes were digested with XbaI/HindIII and the fragments were cloned in the SpeI/HindIII sites of pTR285 vector, yielding p285-LPPα and p285-LPPβ.
To complement the S. coelicolor double mutant strain SC_1153, we amplified SCO1102 and SCO1753 genes plus 300 or 500 bp upstream sequences (in order to include the native promoter) with primers P1102_F/R and P1753_F/R, respectively. The resulting PCR products were cloned in pCR®-BluntII-TOPO vector and checked by DNA sequencing. These sequences were cloned as NotI/SpeI digestion fragments in pRT802 vector, yielding plasmids pRT802-LPPα and pRT802-LPPβ.
All the plasmids described in this section are listed in Table 2.
Membrane preparation of E. coli and S. coelicolor strains
E. coli C41 strains harbouring plasmid pBAD-LPPα, pBAD-LPPβ and pBAD-LPPγ were grown in LB at 37°C until OD600nm 0.6. Then, protein expression was induced by addition of L-arabinose 0.2% and it was followed by overnight incubation at 23°C with gently shaking. Cells were harvested by centrifugation at 4,000 × g for 20 min at 4°C, washed twice with Buffer A (Tris–HCl pH 7.5 50 mM, NaCl 100 mM, EDTA 1 mM, β-mercaptothanol 10 mM) and resuspended in the same buffer. The next steps were all done at 4°C. Disruption of the cells was carried out by sonication (Vibra-Cell™, Sonics & Material Inc.), in the presence of 1 mM phenylmethylsulfonyl fluoride (PMSF) and avoiding foaming. The lysate was cleared by centrifugation at 15,000 × g for 30 min to separate cell debris, and the supernatant was ultracentrifuged at 120,000 × g for 2 h to pellet the membrane fraction. The resulting pellet was washed twice with buffer B (Tris–HCl pH 7.5 20 mM, β-mercaptothanol 10 mM, PMSF 0.5 mM), and resuspended in the same buffer. Protein concentration was quantified by Lowry assay using BSA as standard.
S. coelicolor strains were grown in the corresponding media indicated for each experiment. Cells were harvested by centrifugation at 4,000 × g for 20 min at 4°C washed twice with Buffer A and resuspended in the same buffer. The next steps were done as described above for E. coli.
SDS-PAGE and immunoblot
SDS-PAGE and immunoblot analysis using nitrocellulose membranes were carried out using standard protocols[53, 56]. For detection of the His-tagged proteins, mouse monoclonal anti-His antibodies (QIAGEN™) were used at a dilution of 1:1,000. Anti-mouse IgG-alkaline phosphatase conjugates were used as secondary antibodies at a dilution of 1:3,000. His-tagged proteins were visualized by immunoblots using chromogenic detection as described by the manufacturer.
Phosphatidic acid phosphatase activity assay
To test the phosphatase activity of the putative PAPs of S. coelicolor we used phosphatidic acid as the enzyme substrate. The diacylglycerol generated on the reaction was measured by LC-MS/MS. Standard phosphatase assays were performed in a 100 μL reaction mixture containing 25 mM Tris–HCl, pH 7.5, 2.5 mM Triton X-100 detergent and dipalmitoylphosphatidic acid (DPPA; Avanti Polar Lipids, Alabama, USA) 0.25 mM as substrate. Aliquots of membrane fractions of the corresponding strain were added to initiate the reaction, and after incubation at 30°C the reactions were quenched by adding methanol:chloroform (2:1). Subsequent lipid extraction was performed by the addition of chloroform and distilled water. The organic phase was dried, solubilised in 50 μL of mobile phase and 5 μL aliquots were injected for HPLC and LC-MS/MS analysis. The organic extracts were separated on a ZORBAX Eclipse XDB-C8 column (3.0 × 50 mm, particle size = 1.8 μm; Agilent, USA) using a binary solvent system of water (Solvent A) and methanol (Solvent B). A linear gradient from 80% B to 100% B was applied between 0 and 25 minutes. Both solvents were supplemented with 5 mM ammonium acetate. The outlet of the liquid chromatograph was connected to a micrOTOF mass spectrometer (Bruker Daltonik, Bremen, Germany) operating in the positive-ion mode. The data was acquired online in the mass range m/z 35–1000. Dipalmitoylglycerol (DPG) was detected as a the transition [M + NH4]+ → [M-R-OH]+ ion (m/z 586.5 → m/z 313.3). A calibration curve was done using DPG as standard (Avanti Polar Lipids, Alabama, USA), in the same conditions as the phosphatase reaction. DAG concentration in the samples was calculated by the linear regression equation obtained from the calibration curve.
A unit of enzymatic activity was defined as the amount of enzyme that catalyzed the formation of 1 nmol of product/min. Specific activity was defined as units/mg of protein. PAP activity was linear-dependent to time and protein concentration within the range tested.
Total lipid analysis
E. coli strains harboring pBAD-LPPα, pBAD-LPPβ and pBAD-LPPγ were grown in LB media at 37°C until OD600nm 0.6. Then, protein expression was induced by addition of L-arabinose 0.2%, 3 μCi [14C]-acetate was added at the same time and the culture was kept overnight at 23°C. For Sco0958-PAPs co-expression experiment, cells at OD600nm 0.6 were induced by L-arabinose 0.2% and IPTG 0.1 mM. 3 μCi [14C]-acetate was added to the culture, and it was kept overnight at 23°C. Total lipids of E. coli strains were extracted as described by Bligh & Dyer directly from 14C labeled cells.
For S. coelicolor strains, total lipids were extracted twice from lyophilized cell material (2 mg) with chloroform/methanol (2:1 v/v) as previously described. For de novo lipid biosynthesis, S. coelicolor was grown in R5 liquid media and 3 ml of culture was labeled for 3 h with 3 μCi [14C]-acetate (58.9 mCi/mmol, PerkinElmer).
The lipid extracts were dried and analyzed by TLC on silica gel 60 F254 plates (0 ± 2 mm, Merck), using the solvent systems hexane/diethylether/acetic acid (70:30:1, v/v/v). Lipid fractions were visualized by Cu-phosphoric staining and identified by comparing to the mobility of known standards. For 14C labeled lipids the radioactivity incorporated into each lipid fraction was quantified using a Storm 860 PhosphorImager (Molecular Dynamics) and the corresponding spots were quantified using ImageQuant software (version 5.2).
We are grateful to Gustavo Millán for technical assistance with the LC-MS/MS experiments; to Gabriela Gago and Eduardo Rodriguez for helpful comments on the manuscript; and to Fundación Josefina Prats for a small grant to SC. We thank Gil-Soo Han and George M. Carman for kindly providing us the GHY58 yeast strain. This work was supported by ANPCyT Grants PICT2008-1640 and PICT 2011–2005.
- Alvarez HM, Steinbuchel A: Triacylglycerols in prokaryotic microorganisms. Appl Microbiol Biotechnol. 2002, 60: 367-376. 10.1007/s00253-002-1135-0View ArticleGoogle Scholar
- Barksdale L, Kim KS: Mycobacterium. BacteriolRev. 1977, 41: 217-372.Google Scholar
- Alvarez HM, Kalscheuer R, Steinbuchel A: Accumulation and mobilization of storage lipids by Rhodococcus opacus PD630 and Rhodococcus ruber NCIMB 40126. Appl Microbiol Biotechnol. 2000, 54: 218-223. 10.1007/s002530000395View ArticleGoogle Scholar
- Alvarez HM, Mayer F, Fabritius D, Steinbuchel A: Formation of intracytoplasmic lipid inclusions by Rhodococcus opacus strain PD630. Arch Microbiol. 1996, 165: 377-386. 10.1007/s002030050341View ArticleGoogle Scholar
- Olukoshi ER, Packter NM: Importance of stored triacylglycerols in Streptomyces: possible carbon source for antibiotics. Microbiology. 1994, 140 (Pt 4): 931-943.View ArticleGoogle Scholar
- Liu Q, Siloto RM, Lehner R, Stone SJ, Weselake RJ: Acyl-CoA:diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology. Prog Lipid Res. 2012, 51: 350-377. 10.1016/j.plipres.2012.06.001View ArticleGoogle Scholar
- Kalscheuer R, Steinbuchel A: A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1. J Biol Chem. 2003, 278: 8075-8082. 10.1074/jbc.M210533200View ArticleGoogle Scholar
- Stahl U, Carlsson AS, Lenman M, Dahlqvist A, Huang B, Banas W, Banas A, Stymne S: Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis. Plant Physiol. 2004, 135: 1324-1335. 10.1104/pp.104.044354View ArticleGoogle Scholar
- Stobart K, Mancha M, Lenman M, Dahlqvist A, Stymne S: Triacylglycerols are synthesised and utilized by transacylation reactions in microsomal preparations of developing safflower (Carthamus tinctorius L.) seeds. Planta. 1997, 203: 58-66.Google Scholar
- Fraser T, Waters A, Chatrattanakunchai S, Stobart K: Does triacylglycerol biosynthesis require diacylglycerol acyltransferase (DAGAT)?. Biochemical Society Transactions. 2000, 28: 698-700. 10.1042/BST0280698View ArticleGoogle Scholar
- Borkenhagen LF, Kennedy EP: The enzymatic synthesis of cytidine diphosphate choline. J Biol Chem. 1957, 227: 951-962.Google Scholar
- Waltermann M, Stoveken T, Steinbuchel A: Key enzymes for biosynthesis of neutral lipid storage compounds in prokaryotes: properties, function and occurrence of wax ester synthases/acyl-CoA: diacylglycerol acyltransferases. Bio chimie. 2007, 89: 230-242.Google Scholar
- Zhang YM, Rock CO: Membrane lipid homeostasis in bacteria. Nat Rev Microbiol. 2008, 6: 222-233. 10.1038/nrmicro1839View ArticleGoogle Scholar
- Kalscheuer R: Genetics of Wax Ester and Triacylglycerol Biosynthesis in Bacteria. Handbook of Hydrocarbon and Lipid Microbiology. Edited by: KN T. 2010, Berlin Heidelberg: Springer,Google Scholar
- Kocsis MG, Weselake RJ: Phosphatidate phosphatases of mammals, yeast, and higher plants. Lipids. 1996, 31: 785-802. 10.1007/BF02522974View ArticleGoogle Scholar
- Carman GM, Henry SA: Special issue: Regulation of lipid metabolism in yeast. Bio chim Biophys Acta. 2007, 1771: 239-240. 10.1016/j.bbalip.2006.11.001. 10.1016/j.bbalip.2006.11.001Google Scholar
- Carman GM, Han GS: Roles of phosphatidate phosphatase enzymes in lipid metabolism. Trends Biochem Sci. 2006, 31: 694-699. 10.1016/j.tibs.2006.10.003View ArticleGoogle Scholar
- Jamal Z, Martin A, Gomez-Munoz A, Hales P, Chang E, Russell JC, Brindley DN: Phosphatidate phosphohydrolases in liver, heart and adipose tissue of the JCR:LA corpulent rat and the lean genotypes: implications for glycerolipid synthesis and signal transduction. Int J Obes Relat Metab Disord. 1992, 16: 789-799.Google Scholar
- Carrasco S, Merida I: Diacylglycerol, when simplicity becomes complex. Trends Biochem Sci. 2007, 32: 27-36. 10.1016/j.tibs.2006.11.004View ArticleGoogle Scholar
- Reue K, Brindley DN: Thematic Review Series: Glycerolipids. Multiple roles for lipins/phosphatidate phosphatase enzymes in lipid metabolism. J Lipid Res. 2008, 49: 2493-2503. 10.1194/jlr.R800019-JLR200View ArticleGoogle Scholar
- Donkor J, Zhang P, Wong S, O'Loughlin L, Dewald J, Kok BP, Brindley DN, Reue K: A conserved serine residue is required for the phosphatidate phosphatase activity but not the transcriptional coactivator functions of lipin-1 and lipin-2. J Biol Chem. 2009, 284: 29968-29978. 10.1074/jbc.M109.023663View ArticleGoogle Scholar
- Han GS, Carman GM: Characterization of the human LPIN1-encoded phosphatidate phosphatase isoforms. J Biol Chem. 2010, 285: 14628-14638. 10.1074/jbc.M110.117747View ArticleGoogle Scholar
- Brindley DN, Pilquil C: Lipid phosphate phosphatases and signaling. J Lipid Res. 2009, 50 (Suppl): S225-S230.Google Scholar
- Carman GM: Phosphatidate phosphatases and diacylglycerol pyrophosphate phosphatases in Saccharomyces cerevisiae and Escherichia coli. Biochim Biophys Acta. 1997, 1348: 45-55. 10.1016/S0005-2760(97)00095-7View ArticleGoogle Scholar
- Han GS, Wu WI, Carman GM: The Saccharomyces cerevisiae Lipin homolog is a Mg2 + −dependent phosphatidate phosphatase enzyme. J Biol Chem. 2006, 281: 9210-9218.View ArticleGoogle Scholar
- Toke DA, Bennett WL, Dillon DA, Wu WI, Chen X, Ostrander DB, Oshiro J, Cremesti A, Voelker DR, Fischl AS, Carman GM: Isolation and characterization of the Saccharomyces cerevisiae DPP1 gene encoding diacylglycerol pyrophosphate phosphatase. J Biol Chem. 1998, 273: 3278-3284. 10.1074/jbc.273.6.3278View ArticleGoogle Scholar
- Toke DA, Bennett WL, Oshiro J, Wu WI, Voelker DR, Carman GM: Isolation and characterization of the Saccharomyces cerevisiae LPP1 gene encoding a Mg2 + −independent phosphatidate phosphatase. J Biol Chem. 1998, 273: 14331-14338. 10.1074/jbc.273.23.14331View ArticleGoogle Scholar
- Han GS, Johnston CN, Chen X, Athenstaedt K, Daum G, Carman GM: Regulation of the Saccharomyces cerevisiae DPP1-encoded diacylglycerol pyrophosphate phosphatase by zinc. J Biol Chem. 2001, 276: 10126-10133. 10.1074/jbc.M011421200View ArticleGoogle Scholar
- Han GS, Johnston CN, Carman GM: Vacuole membrane topography of the DPP1-encoded diacylglycerol pyrophosphate phosphatase catalytic site from Saccharomyces cerevisiae. J Biol Chem. 2004, 279: 5338-5345.View ArticleGoogle Scholar
- Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK: Global analysis of protein localization in budding yeast. Nature. 2003, 425: 686-691. 10.1038/nature02026View ArticleGoogle Scholar
- Wu WI, Liu Y, Riedel B, Wissing JB, Fischl AS, Carman GM: Purification and characterization of diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae. J Biol Chem. 1996, 271: 1868-1876. 10.1074/jbc.271.4.1868View ArticleGoogle Scholar
- Furneisen JM, Carman GM: Enzymological properties of the LPP1-encoded lipid phosphatase from Saccharomyces cerevisiae. Bio chim Biophys Acta. 2000, 1484: 71-82. 10.1016/S1388-1981(99)00202-4. 10.1016/S1388-1981(99)00202-4Google Scholar
- Dillon DA, Wu WI, Riedel B, Wissing JB, Dowhan W, Carman GM: The Escherichia coli pgpB gene encodes for a diacylglycerol pyrophosphate phosphatase activity. J Biol Chem. 1996, 271: 30548-30553. 10.1074/jbc.271.48.30548View ArticleGoogle Scholar
- Icho T, Raetz CR: Multiple genes for membrane-bound phosphatases in Escherichia coli and their action on phospholipid precursors. J Bacteriol. 1983, 153: 722-730.Google Scholar
- Touze T, Blanot D, Mengin-Lecreulx D: Substrate specificity and membrane topology of Escherichia coli PgpB, an undecaprenyl pyrophosphate phosphatase. J Biol Chem. 2008, 283: 16573-16583. 10.1074/jbc.M800394200View ArticleGoogle Scholar
- Lu YH, Guan Z, Zhao J, Raetz CR: Three phosphatidylglycerol-phosphate phosphatases in the inner membrane of Escherichia coli. J Biol Chem. 2011, 286: 5506-5518. 10.1074/jbc.M110.199265View ArticleGoogle Scholar
- Nakamura Y, Tsuchiya M, Ohta H: Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J Biol Chem. 2007, 282: 29013-29021. 10.1074/jbc.M704385200View ArticleGoogle Scholar
- Punta M, Coggill PC, Eberhardt RY, Mistry J, Tate J, Boursnell C, Pang N, Forslund K, Ceric G, Clements J: The Pfam protein families database. Nucleic Acids Res. 2012, 40: D290-D301. 10.1093/nar/gkr1065View ArticleGoogle Scholar
- Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR: CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res. 2011, 39: D225-D229. 10.1093/nar/gkq1189View ArticleGoogle Scholar
- BLAST: Basic Local Alignment Search Tool.http://www.ncbi.nlm.nih.gov/BLAST/,
- FASTA/SSEARCH/GGSEARCH/GLSEARCH - Protein Similarity Search.http://www.ebi.ac.uk/Tools/sss/fasta/,
- Nugent T, Jones DT: Transmembrane protein topology prediction using support vector machines. BMC Bioinformatics. 2009, 10: 159-, 10.1186/1471-2105-10-159View ArticleGoogle Scholar
- Guzman LM, Belin D, Carson MJ, Beckwith J: Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995, 177: 4121-4130.Google Scholar
- Wagner S, Klepsch MM, Schlegel S, Appel A, Draheim R, Tarry M, Hogbom M, van Wijk KJ, Slotboom DJ, Persson JO, de Gier JW: Tuning Escherichia coli for membrane protein overexpression. Proc Natl Acad Sci U S A. 2008, 105: 14371-14376. 10.1073/pnas.0804090105View ArticleGoogle Scholar
- Mumberg D, Muller R, Funk M: Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene. 1995, 156: 119-122. 10.1016/0378-1119(95)00037-7View ArticleGoogle Scholar
- Kieser T, Bibb M, Buttner MJ, Chater KF, Hopwood DA: Practical Streptomyces genetics. 2000, Norwich, United Kingdom: The John Innes Foundation,Google Scholar
- Zavaleta-Pastor M, Sohlenkamp C, Gao JL, Guan Z, Zaheer R, Finan TM, Raetz CR, Lopez-Lara IM, Geiger O: Sinorhizobium meliloti phospholipase C required for lipid remodeling during phosphorus limitation. Proc Natl Acad Sci U S A. 2010, 107: 302-307. 10.1073/pnas.0912930107View ArticleGoogle Scholar
- Hanahan D: Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983, 166: 557-580. 10.1016/S0022-2836(83)80284-8View ArticleGoogle Scholar
- Paget MS, Leibovitz E, Buttner MJ: A putative two-component signal transduction system regulates sigmaE, a sigma factor required for normal cell wall integrity in Streptomyces coelicolor A3(2). Mol Microbiol. 1999, 33: 97-107. 10.1046/j.1365-2958.1999.01452.xView ArticleGoogle Scholar
- Thomas BJ, Rothstein R: Elevated recombination rates in transcriptionally active DNA. Cell. 1989, 56: 619-630. 10.1016/0092-8674(89)90584-9View ArticleGoogle Scholar
- Gregory MA, Till R, Smith MC: Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors. J Bacteriol. 2003, 185: 5320-5323. 10.1128/JB.185.17.5320-5323.2003View ArticleGoogle Scholar
- Arabolaza A, Rodriguez E, Altabe S, Alvarez H, Gramajo H: Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor. Appl Environ Microbiol. 2008, 74: 2573-2582. 10.1128/AEM.02638-07View ArticleGoogle Scholar
- Sambrock J, Fritsch FEF, Maniatis T: Molecular cloning: a laboratory manual. 1989, Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory Press,Google Scholar
- Bishop A, Fielding S, Dyson P, Herron P: Systematic insertional mutagenesis of a streptomycete genome: a link between osmoadaptation and antibiotic production. Genome Res. 2004, 14: 893-900. 10.1101/gr.1710304View ArticleGoogle Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem. 1951, 193: 265-275.Google Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. 10.1038/227680a0View ArticleGoogle Scholar
- Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959, 37: 911-917.View ArticleGoogle Scholar
- Arabolaza A, D'Angelo M, Comba S, Gramajo H: FasR, a novel class of transcriptional regulator, governs the activation of fatty acid biosynthesis genes in Streptomyces coelicolor. Mol Microbiol. 2010, 78: 47-63.Google Scholar
- Rotering H, Raetz CR: Appearance of monoglyceride and triglyceride in the cell envelope of Escherichia coli mutants defective in diglyceride kinase. J Biol Chem. 1983, 258: 8068-8073.Google Scholar
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