Table 2 Strains and plasmids

Strains

S. coelicolor

 M145

Parental strain, SCP1^- SCP2^-

[46]

 SC_1102

SCO1102::Tn5062, derivative of M145; Am^R

This study

 SC_1753

SCO1753::Tn5062, derivative of M145; Am^R

This study

 SC_1153

SCO1102::Tn5062 SCO1753::Tn5066, derivative of SC_1102; Am^R Hyg^R

This study

 SC_285

M145 attB _ΦBT1 ::pTR285; Km^R

This study

 SC_Lppα

M145 attB _ΦBT1 ::p285-LPPα; Km^R

This study

 SC_Lppβ

M145 attB_{ΦBT 1}::p285-LPPβ; Km^R

This study

 SC_P1102

SC_1153 attB _ΦBT1 ::pRT802-LPPα; Am^R Hyg^R Km^R

This study

 SC_P1753

SC_1153 attB_{ΦBT 1}::pRT802-LPPβ; Am^R Hyg^R Km^R

This study

E. coli

 DH5α

E. coli K12 F ^- lacU169 (Φ80lacZΔM15) endA1 recA1 hsdR17 deoR supE44 thi-1 l2 gyrA96 relA1

[48]

 BL21 (DE3)

F ^– ompT gal dcm lon hsdS _B (r _B ^- m _B ^- ) λ(DE3)

Novagen

 C41 (DE3)

F ^– ompT gal dcm lon hsdS _B (r _B ^- m _B ^- ) λ(DE3)

Lucigen

 ET 12567

supE44 hsdS20 ara^-14 proA2 lacY galK2 rpsL20 xyl^-5 mtl^-1 dam^-dcm^-hsdM^-; Cm^R

[49]

S. cerevisiae

 W303-1A

MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1

[50]

 GHY58

W303-1A dpp1 _::TRP1/Kan^Rlpp1 _::HIS3/Kan^Rpah1 _::URA3

[25]

Plasmids

 pET28a

Vector for expression of N terminal His-tagged proteins under the strong T7 promoter; Km^R

Novagen

 pBAD33

Vector for recombinant protein expression under the control of the P _BAD promoter; Cm^R

[43]

 pCR®-BluntII-TOPO

Vector used for cloning of blunt PCR products; Km^R

Invitrogen

 pUZ8002

RK2 derivative with defective oriT; Km^R

[49]

 pKOS111-47

RK2 derivative with defective oriT; Ap^R

B. Julien (Personal communication)

 pQM5066

Plasmid carrying a copy of Tn5066; Hyg^R

P. Dyson, (Personal communication)

 pRT802

Intregrative vector based on ΦBT1 phage integrase; Km^R

[51]

 p425GPD

Multicopy Yeast/E. coli expression vector with GPD promoter and LEU2 marker; Ap^R

[45]

 pTR285

pRT802 derivative carrying the P _ermE* promoter with no gene under its control; Km^R

[52]

 pBAD0958

pBAD33 carrying the SCO0958^His gene under the control of P _BAD promoter; Cm^R

[52]

 pBAD-LPPα

pBAD33 carrying the SCO1102^His gene under the control of P _BAD promoter; Cm^R

This study

 pBAD-LPPβ

pBAD33 carrying the SCO1753^His gene under the control of P _BAD promoter; Cm^R

This study

 pBAD-LPPγ

pBAD33 carrying the SCO6355^His gene under the control of P _BAD promoter; Cm^R

This study

 p425-LPPα

p425-GPD carrying the SCO1102^His gene under the control of GPD promoter; Ap^R, LEU2

This study

 p425-LPPβ

p425-GPD carrying the SCO1753^His gene under the control of GPD promoter; Ap^R, LEU2

This study

 p425-LPPγ

p425-GPD carrying the SCO6355^His gene under the control of GPD promoter; Ap^R, LEU2

This study

 p28-LPPα

pET28 carrying the SCO1102^His gene under the control of T7 promoter; Km^R

This study

 p28-LPPβ

pET28 carrying the SCO1753^His gene under the control of T7 promoter; Km^R

This study

 p285-LPPα

pTR285 carrying the SCO1102^His gene under the control of P _ermE* promoter; Km^R

This study

 p285-LPPβ

pTR285 carrying the SCO1753^His gene under the control of P _ermE* promoter; Km^R

This study

 pRT802-LPPα

pRT802 carrying the SCO1102^His gene under the control of its own promoter; Km^R

This study

 pRT802-LPPβ

pRT802 carrying the SCO1753^His gene under the control of its own promoter; Km^R

This study