- Open Access
Production of hydroxycinnamoyl-shikimates and chlorogenic acid in Escherichia coli: production of hydroxycinnamic acid conjugates
© Kim et al; licensee BioMed Central Ltd. 2013
Received: 11 December 2012
Accepted: 2 February 2013
Published: 5 February 2013
Hydroxycinnamates (HCs) are mainly produced in plants. Caffeic acid (CA), p-coumaric acid (PA), ferulic acid (FA) and sinapic acid (SA) are members of the HC family. The consumption of HC by human might prevent cardiovascular disease and some types of cancer. The solubility of HCs is increased through thioester conjugation to various compounds such as quinic acid, shikimic acid, malic acid, anthranilic acid, and glycerol. Although hydroxycinnamate conjugates can be obtained from diverse plant sources such as coffee, tomato, potato, apple, and sweet potato, some parts of the world have limited availability to these compounds. Thus, there is growing interest in producing HC conjugates as nutraceutical supplements.
Hydroxycinnamoyl transferases (HCTs) including hydroxycinnamate-CoA shikimate transferase (HST) and hydroxycinnamate-CoA quinate transferase (HQT) were co-expressed with 4-coumarateCoA:ligase (4CL) in Escherichia coli cultured in media supplemented with HCs. Two hydroxycinnamoyl conjugates, p-coumaroyl shikimates and chlorogenic acid, were thereby synthesized. Total 29.1 mg/L of four different p-coumaroyl shikimates (3-p-coumaroyl shikimate, 4-p-coumaroyl shikimate, 3,4-di-p-coumaroyl shikimate, 3,5-di-p-coumaroyl shikimate, and 4,5-di-p-coumaroyl shikimate) was obtained and 16 mg/L of chlorogenic acid was synthesized in the wild type E. coli strain. To increase the concentration of endogenous acceptor substrates such as shikimate and quinate, the shikimate pathway in E. coli was engineered. A E. coli aroL and aroK gene were mutated and the resulting mutants were used for the production of p-coumaroyl shikimate. An E. coli aroD mutant was used for the production of chlorogenic acid. We also optimized the vector and cell concentration optimization.
To produce p-coumaroyl-shikimates and chlorogenic acid in E. coli, several E. coli mutants (an aroD mutant for chlorogenic acid production; an aroL, aroK, and aroKL mutant for p-coumaroyl-shikimates production) were made and each mutant was tested using an optimized construct. Using this strategy, we produced 235 mg/L of p-coumaroyl-shikimates and 450 mg/L of chlorogenic acid.
Hydroxycinnamates (HCs) such as caffeic acid (CA), p-coumaric acid (PA), ferulic acid (FA) and sinapic acid (SA) are mainly produced in plants. Because HCs are anti-oxidants that can scavenge free radicals, it has been suggested that consumption of HCs might prevent cardiovascular disease and some types of cancer. In addition, several biological functions of HCs have been described, such as protection against side effects of chemotheraphy and anti-osteoclast activity .
In plants, HCs are usually conjugated with other compounds such as quinic acid, shikimic acid, malic acid, anthranilic acid, and glycerol . Chlorogenic acid, the conjugate of CA and quinate, is abundant in coffee, fruits, and vegetables, and is the primary source of CA in the human diet . For regular consumers of coffee in Western countries, the major dietary phenolics are chlorogenic acids .
The solubility of HCs is increased through thioester conjugation to various compounds. Although there is growing evidence for their beneficial health effects, the fruits and vegetables that contain high levels of HC conjugates are of limited availability in some parts of the world. Thus, there is growing interest in producing HC conjugates as nutraceutical supplements. The primary strategy for obtaining diverse phytochemicals is by extracting them from plants. However, this approach faces several obstacles such as a limited availability of plant materials and high costs of extraction and purification. Alternatively, they can be produced in microorganisms. Phytochemicals such as flavonoids and stilbenes have been produced using microorganisms such as Escherichia coli and Saccharomyces cerevisiae[5–7].
Biosynthesis of HC conjugates is mediated by hydroxycinnamoyl transferases (HCTs), which use the coenzyme A thioester of HCs as a donor and various compounds such as quinate, and shikimate as HC acceptors. The formation of coenzyme A thioester with HC is catalyzed by 4-coumaric acid:CoA ligase (4-CL) . Thus, genes encoding 4-CL and HCT, are essential for producing HC conjugates from HC. HCTs from several plants have been characterized [8–12]. HCTs show specificity for not only the acyl group donor but also the acyl group acceptor. When the acyl group acceptors are shikimate and quinate, the resulting conjugates are p-coumaroyl-O-shikimate and chlorogenic acid, respectively [13, 14].
E. coli is a good system for producing plant secondary metabolites including phytochemicals produced through the phenylpropanoid pathway . We attempted to produce HC conjugates using E. coli. Shikimate and 3-dehydroquinate, which are acyl group acceptors for hydroxycinnamate-CoA shikimate transferases (HST) and hydroxycinnamate-CoA quinate transferases (HQT), respectively, can be synthesized through the shikimate pathway of E. coli[16, 17]. Thus, it should be possible to alter the shikimate pathway to increase the concentrations of shikimate and quinate. However, the acyl donor (PA or CA) is a unique product of plants . In order to produce HCs in E. coli, coexpression of several genes in E. coli is necessary, which leads to metabolic load. Therefore addition of exogenous HCs may circumvent this problem. In this report, we introduced either HST and 4CL (for the production of CA-shikimate), or HQT and 4CL (for the production of chlorogenic acid) into E. coli mutants that accumulate either shikimate or 3-hydroquinate. When the engineered cells were fed exogenous HCs, a high yield of HC-shikimate and HC-quinate were obtained.
Construction and selection of the optimum expression vector for the synthesis of HC-shikimate
Plasmids, Escherichia coli strains, and primers used in this study
Plasmids or E. coli strains or Primers
Relevant properties or genetic marker
Source or reference
P15A ori, Cmr
CloDE13 ori, Strr
f1 ori, Ampr
pACYCDDuet carrying NtHST from N. tobacco and 4CL from O. sativa
pCDFDuet carrying NtHST from N. tobacco and 4CL from O. sativa
pETDuet carrying NtHST from N. tobacco and 4CL from O. sativa
pCDFDuet carrying NtHQT from N. tobacco and 4CL from O. sativa
pACYC carrying ydiB from E. coli
F-ompT hsdS B (rB- mB-) gal dcm lon (DE3)
BaroD carrying pCDF-Duet
BaroD carrying pC-NtHQT-Os4CL
BaroD carrying pC-NtHQT-Os4CL and pA-EcydiB
BL21(DE3) ΔaroD::FRT-kan R -FRT
BL21(DE3) ΔaroK::FRT-kan R -FRT
BL21(DE3) ΔaroL::FRT-kan R -FRT
BL21(DE3) ΔaroK::FRT ΔaroL::FRT-kan R -FRT
AACATATG AAGATCGAAGTGAAAGAAT (NdeI site is underlined)
AACTCGAG TCAAAAGTCATACAAGAACTTC (XhoI site is underlined)
AAGATATC CATGGGAAGTGAAAAAATGATGA (EcoRV site is underlined)
AAGGTACC TCAAAATTCATACAAATACTT (KpnI site is underlined)
ATGAATTC GATGGATGTTACCGCAAAATAC (EcoR I site is underlined)
CATGCGGCCGC TCAGGCACCGAACCCCATG (Not I site is underlined)
aroK or aroL check-R
Based on the molecular masses of P2, P3, and P4 (Figure 2C), they contained two PAs and one shikimate. Two PAs can be connected to 3"-OH or 4"-OH or 5"-OH of SA, so that there can be three isomers, i.e. 3"-OH/4"-OH, 3"-OH/5"-OH, or 4"-OH/5"-OH. We could not determine which produce was which isomer. By comparison to a previous report , P3 and P4 are likely to be 3,5-di-p-coumaroyl shikimate and 4,5-di-p-coumaroyl shikimate, respectively. The remaining peak, P2 is therefore likely to be 3,4-di-p-coumaroyl shikimate.
Using E. coli harboring pC-NtHST-Os4CL, we determined the best acyl donor among CA, PA, and FA. In a previous study, recombinant NtHST protein most efficiently used caffeoyl-CoA as an acyl donor . In this study, 1 mM of each HC was added to the same number of cells, and biotransformation was performed for 6 h. PA was the most effective acyl donor, producing 23.9 mg/L of p-coumaroyl shikimate. The amounts of feruloyl shikimate and caffeoyl shikimate produced were 3.8 mg/L, and 3.1 mg/L, respectively. Taken together, these results indicate that the highest amount of HC-shikimate was obtained with pC-NtHST-Os4CL as the construct and PA as the substrate.
Engineering E. coli to increase production of PA-shikimate conjugates
Next, production of PA-shikimate was optimized using BaroL harboring pC-NtHST-Os4CL. The optimum cell concentration was determined by varying the cell concentration from OD600 1 to 5 in the presence of 1 mM PA. Production of PA-shikimate peaked at an OD600 of 3, at which cell concentration the yield of PA-shikimate was approximately 235 mg/L. At OD600 = 1, or 2, the yield was approximately 94 mg/L, and 169 mg/L, respectively. Above OD600 = 3, the production of PA-shikimate decreased and was approximately 188 or 103.7 mg/L at OD600 = 4 or 5, respectively.
Production of HC-quinate in E. coli
The protein product of ydiB converts 3-dehydroquinate into quinate . ydiB was overexpressed in E. coli strain B-101 to make strain B-102. Biotransformation of CA by strain B-102 resulted in a new product with an identical HPLC retention time and molecular mass (354 Da) as chlorogenic acid (Figure 5D, F). The MS/MS spectrum of the reaction product matched authentic chlorogenic acid. This indicates that ydiB converts 3-dehydroquinate into quinate, which was then utilized for the production of chlorogenic acid.
PA and FA were also tested as acyl-group donors. CA was the best acyl-group donor followed by PA and FA. After 24 h, the amounts of caffeoyl-quinate, p-coumaroyl-quinate, and feruloyl-quinate were 450 mg/L, 323.7 mg/L, and 216 mg/L, respectively.
Anthocyanins, flavonoids, stilbene, and other compounds of plant origins have been biologically synthesized using engineered E. coli strains [7, 15]. Most of these efforts involved introduction of new genes with high activity into E. coli. In addition, cofactor supplementation and reducing equivalents have been enhanced to produce natural compounds using engineered E. coli[6, 28]. The goal of this study was to produce PA-shikimate and chlorogenic acid in E. coli. We also engineered the shikimate pathway of E. coli to accumulate the acyl group acceptors, shikimate and quinate, which led to the increased production of PA-shikimates and chlorogenic acid.
During biotransformation, two PA molecules are attached to shikimate, whereas only one molecule of CA is attached to quinate. In plants, one molecule of CA is bound to one molecule of shikimate. However, diverse HC-quinate conjugates, including those in which two or three identical HCs are bound to quinate, and two or three different HCs are bound to quinate, were found in coffee (Coffea robusta) and sweet potato (Ipomonea batatas) [21, 29]. In these plants, the mono-esters are present as major components whereas di- and tri-esters are present as minor components. NtHST and NtHQT may have different enzymatic properties from HCTs from coffee or sweet potato because di-p-coumaroyl skimates and cholorgenic acid were synthesized using NtHST and NtHQT. One possible scenario is that the substrate binding pocket of NtHQT may be narrower than that of NtHST. Thus, HC-quinate may not fit into the substrate binding pocket of NtHQT for the second round of acyl transfer reaction. Longer incubation of the recombinant NtHST enzyme with PA-CoA and shikimate resulted in the production of di-PA-shikimate (data not shown). The production of di-PA-shikimate was also observed with HST from Populus euramericana. Using the properties of NtHST, diverse HC-shikimate conjugates could possibly be obtained and feeding of different concentrations of various HCs into the E. coli culture medium may result in production of various forms of HC-shikimate conjugates. Any biological activity including anti-oxidant activity of di-PA-shikimate compared to PA-shikimate has not been tested. However, 8-O-4-diferulic acid showed better antioxidant activity than ferulic acid .
p-Coumaric acid can be synthesized from tyrosine by the action of tyrosine ammonia lyase (TAL). Therefore, synthesis of p-coumaroyl-shikimate or chlorogenic acid from glucose might be possible if a gene econding TAL were expressed into E. coli harboring NtHST and Os4CL or into E. coli harboring NtHQT, Os4CL, ydiB, and Sam5 (Sam5 converts p-coumaric acid into caffeic acid ), respectively. However, it is expected that only a tiny amount of reaction product would be produced from glucose in a wild type strain because quinate and shikimate are not accumulated in the wild type E. coli. Use of strain BaroD or BaroL is not also feasible because tyrosine is not synthesized in these strains. Thus, supplementation with either caffeic acid or tyrosine is needed to synthesize p-coumaroyl-shikimate or chlorogenic acid in E. coli.
Wild type E. coli expressing NtHQT and Os4CL did not produce detectable amounts of chlorogenic acid or caffeoyl-dehydroquinate after CA supplementation. In the wild type, dehydroquinate may have been rapidly converted into another downstream compound of the shikimate pathway instead of accumulating. Moreover, the expression level of ydiB, which converts dehydroquinate into quinate, was low. Only a small amount of quinate or dehydroquinate will be expected to be present in the cell in the absence of overexpression of ydiB. ydiB and aroE mediate the conversion reaction not only from dehydroquinate to quinate but also from dehydroshikimate to shikimate. However, aroE prefers dehydroshikimate to dehydroquinate , whereas ydiB has nearly the same catalytic efficiency for dehydroshikimate and dehydroquinate [27, 33]. Recent studies have shown that overexpression of ydiB does not increase shikimate, while overexpression of aroE increases conversion of dehydroshikimate to shikimate . Therefore, we overexpressed ydiB to produce chlorogenic acid. In addition, strains BaroK, BaroL, and BaroKL expressing pC-NtHQT-Os4CL that were supplemented with CA produced chlorogenic acid only when ydiB was overexpressed (data not shown). This indicated that these mutants accumulate shikimate pathway intermediates such as dehydroquinate, dehydroshikimate and shikimate. These intermediates are converted into quiniate by ydiB.
To produce the HC-conjugates in E. coli, several E. coli mutants including aroD, aroK, aroL, and aroK/L mutants were made and each mutant was tested using an optimized construct. For the production of HC-shikimates, an E. coli aroL mutant (B-aroL) was best and we produced 235 mg/L of HC-shikimates using B-aroL expressing NtHST and Os4CL, which is approximately 15-fold higher than wild type E. coli BL21 (16 mg/L). The wild type E. coli expressing NtHQT and Os4CL did not produce any detectable chlorogenic acid. By using E. coli aroD mutant (strain B-101), which accumulated 3-dehydroquinate, caffeoyl-3-dehydroquinate instead of chlorogenic acid, was synthesized. However, by expressing ydiB gene in strain B-101, which converted 3-dehyroquinate to quinate, chlorogenic acid production was dramatically increased up to 450 mg/L.
Materials and methods
Strains and reagents
The E. coli strains used in this study are listed in Table 1. E. coli BL21 (DE3) cells were used for recombinant protein production. E. coli DH5α cells were used for plasmid cloning. All restriction enzymes and T4 DNA ligase were purchased from Takara (Shiga, Japan). Polymerase chain reaction (PCR) amplification was performed using Hotstart Taq DNA polymerase (Qiagen, Hilden Germany). Reverse transcription was performed using Omniscript reverse transcriptase (Qiagen). E. coli was cultured in Luria-Bertani (LB) or M9 medium (plus 2% glucose) containing 50 μg/mL antibiotics, when necessary. E. coli expression vectors were purchased from Novagen (Madison, WI, USA).
Construction of E. coli expression vector
The genes for hydroxycinnamate-CoA shikimate transferase (NtHST)  and hydroxycinnamate-CoA quinate transferase (NtHQT) from tobacco (N. tabacum)  were cloned using reverse-transcription polymerase chain reaction (RT-PCR). Total RNA was isolated from the leaves of one-month-old tobacco using Plant Total RNA Isolation Kit (Qiagen), and cDNA was synthesized using Omniscript reverse transcriptase (Qiagen) and oligo dT as a primer. PCR was carried out using primers designed on the basis of the published sequences (GenBank accession AJ507825 for NtHST and AJ582651 for NtHQT). The primers were listed in Table 1. The Os4CL gene, which was cloned and characterized previously from rice , was subcloned into the Bam HI/Not I sites of pACYCDuet, pCDFDuet, and pETDuet vectors (EMD Chemicals, Gibbstown, NJ, USA), and then the resulting NtHST PCR product was subcloned into a second cloning site, the Nde I/Xho I site of each vector. The NtHQT PCR product was subcloned into the EcoR V/Kpn I site of pCDFDuet, which contains Os4CL at Nde I/Xho I site. The resulting constructs are listed in Table 1.
Deletion of the aroL and aroK genes in E. coli BL21(DE3) was accomplished using the Quick and Easy Conditional Knockout Kit (Gene Bridges, Heidelberg, Germany). Briefly, the aroL gene or the aroK gene of E. coli BL21 (DE3) was replaced by the ΔaroL FRT-PGK-gb2-neo-FRT cassette or the ΔaroK FRT-PGK-gb2-neo-FRT cassette, respectively . Deletion mutants were selected in Luria-Bertani (LB) medium containing 50 μg/mL kanamycin. Deletion of aroL or aroK was confirmed using PCR. The strains deleted in aroL or aroK were named BaroL and BaroK, respectively (Table 1). ΔaroL and ΔaroK double mutant (strain BaroKL in Table 1) was constructed using strain BaroL. The kanamycin cassette was removed in ΔaroL using an FLP expression plasmid, which removes the kanamycin selection marker from the chromosome. Removal of the kanamycin cassette was confirmed by PCR. Using ΔaroL as a host, the aroK gene was replaced by the ΔaroK FRT-PGK-gb2-neo-FRT cassette. The primer sequences for the aroD, aroK, and aroL deletion are shown in Table 1.
Shikimate/quinate dehydrogenase gene, ydiB was cloned using E. coli BL21 (DE3) genomic DNA as a template. Primers were designed based on the published sequence (NC_000913.2) and the sequences of primers were listed in Table 1. The PCR product was digested with EcoR I/Not I and subcloned into the corresponding site of pCDF-Duet1. The resulting constructs were confirmed by sequencing.
Production of HC-shikimate conjugate in E. coli
Each construct was transformed into E. coli BL21 (DE3) strain or BaroL strain using electroporation with the BioRad MicroPulser Electroporation Apparatus (BioRad, Hercules, CA, USA). Overnight cultures of transformants were inoculated into LB medium containing 50 μg/mL of antibiotic at 37°C and cultured until the OD600 reached 0.8. Protein expression was induced by the addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG), and growth was continued for another 20 h at 18°C. Cells were harvested and resuspended to obtain a cell concentration corresponding to OD600 of 3 in 2 mL of fresh M9 medium containing 2% glucose, 50 μg/ mL of antibiotics, 1 mM IPTG, and 1 mM PA in a test tube (14 mm × 145 mm) and then cultured at 30°C for 8 h. The supernatant was extracted twice using an equal volume of ethyl acetate, and the upper aqueous phase was dried using a Speed Vac at 30°C, dissolved in 60 μL of dimethylsulfoxide (DMSO), and analyzed using high-performance liquid chromatography (HPLC). Because p-coumaroyl shikimate is not commercially available, we used p-coumaric acid to generate a standard curve for quantitative analysis of the reaction products. The UV spectra of p-coumaric acid are distinguishable from those of p-coumaroyl shikimate but they are very similar and the absorption of the thioesters is usually shifted to longer wavelength by a few nm. This is subtle enough that it should be perfectly acceptable to use the free acids as quantitation standards for the thioesters.
To determine the maximum conversion rate using the optimized vector, protein expression was induced as described above. The cell concentration was adjusted to an OD600 of 3 in 25 mL of fresh M9 containing 2% glucose, 1 mM IPTG, and 50 μg/mL of antibiotics. The medium was supplemented with 1 mM PA. The reaction product (200 μL) was collected and extracted with ethyl acetate. The supernatant was dried and dissolved with 100 μl of dimethyl sulfoxide (DMSO). The sample was directly injected to HPLC for analysis. The mean and the standard error of the mean were calculated from triplicate experiments. Analysis of variance (ANOVA) was carried out using Tukey’s method with a significance level of P=0.01 using 2010 Microsoft Office Excel.
Production of chlorogenic acid in E. coli
The construct for the production of chlorogenic acid was transformed into E. coli BL21(DE3) or BaroD cells. Induction of each construct was performed as described above. To determine the optimal gene construct, 200 μM PA was added to the growth medium.
To measure the production of chlorogenic acid in BaroD cells harboring pC-EcycdiB and pC-NtHQT-Os4CL (Table 1), the cell concentration was adjusted to an OD600 of 2.0, and CA was added to the medium to a final concentration of 1.5 mM. Production of chlorogenic acid was periodically monitored. The biotransformation was stopped by boiling for 5 min and the biotransformation product was centrifuged for 15 min at 13000 × g to remove the cell debris and other components prior to HPLC analysis. The quantification of the product was carried out using a standard curve generated with authentic chlorogenic acid (Sigma, MO, USA).
Analysis of the metabolites
The metabolites were analyzed using a Varian HPLC equipped with a photo diode array (PDA) detector and a Varian C18 reversed-phase column (Varian, 4.60 × 250 mm, 3.5 μm particle size). The mobile phases consisted of 0.1% formic acid in water and acetonitrile. For chlorogenic acid, the program was: 20% acetonitrile at 0 min, 32% acetonitrile at 15 min, 90% acetonitrile at 17 min, 90% acetonitrile at 20 min, 20% acetonitrile at 21 min, and 20% acetonitrile at 26 min. To analyze hydroxycinnamoyl shikimate, the program was: 25% acetonitrile at 0 min, 40% acetonitrile at 10 min, 75% acetonitrile at 15 min, 90% acetonitrile at 22 min, 25% acetonitrile at 23 min, and 25% acetonitrile at 30 min. The flow rate was 1 mL/min, and the separation was monitored at 290 nm and 320 nm.
The molecular masses of the metabolites were determined using a Varian 500-MS ion trap spectrometer. Mass spectra were acquired simultaneously using an electrospray ionization source in negative ionization mode at 600 V. NMR spectrometry was done as described before .
This work was supported by a grant from Systems and Synthetic Agro-Biotech Center through the Next-Generation BioGreen 21 Program (PJ00948301), Rural Development Administration, and partially funded by the Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012–0006686).
- El-Seedi HR, El-Seed AMA, Khalifa SAM, Görasson U, Bohlin L, Borg-Karlson A-K, Verpoorte R: Biosynthesis, natural sources, dietary intake, pharmacokinetic properties, and biological activities of hydroxycinnamic acids. J Agri Food Chem. 2012, 60 (44): 10877-10895. 10.1021/jf301807g.View ArticleGoogle Scholar
- Clifford MN: Chlorogenic acids and other cinnamates-nature, occurrence and dietary burden. J Sci Food Agric. 1999, 79 (3): 362-372. 10.1002/(SICI)1097-0010(19990301)79:3<362::AID-JSFA256>3.0.CO;2-D.View ArticleGoogle Scholar
- Herrmann K: Occurrence and content of hydroxycinnamic acid and hydoxybenzoic acid compounds in foods. Crit Rev Food Sci Nutr. 1989, 28 (4): 315-347. 10.1080/10408398909527504.View ArticleGoogle Scholar
- Crozier A, Jaganatha IB, Clifford MM: Dietary phenolics: chemistry, bioavailability and effects on health. Nat Prod Rep. 2009, 26 (8): 1001-10043. 10.1039/b802662a.View ArticleGoogle Scholar
- Chemler JA, Koffas MAG: Metabolic engineering for plant natural product biosynthesis in microbes. Cur Opin Biotech. 2008, 19 (6): 597-605. 10.1016/j.copbio.2008.10.011.View ArticleGoogle Scholar
- Chemler JA, Fowler ZL, Mchugh KP, Koffas MAG: Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering. Metab Eng. 2010, 12 (2): 96-104. 10.1016/j.ymben.2009.07.003.View ArticleGoogle Scholar
- Fowler ZL, Koffas MAG: Biosynthesis and biotechnological production of flavanones: current state and perspectives. Appl Microbiol Biotechnol. 2009, 83 (5): 799-808. 10.1007/s00253-009-2039-z.View ArticleGoogle Scholar
- Beuerle T, Pichershy E: Enzymatic synthesis and purification of aromatic coenzyme A esters. Anal Biochem. 2002, 302 (2): 305-312. 10.1006/abio.2001.5574.View ArticleGoogle Scholar
- Hoffmann L, Maury S, Martz F, Geoffroy P, Legrand M: Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism. J Biol Chem. 2003, 278 (1): 95-103.View ArticleGoogle Scholar
- Hoffmann L, Besseau S, Geoffoy P, Ritzenthaler C, Meyer D, Lapierre C, Pollet B, Legrand M: Silencing of hydroxycinnamoyl-Coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis. Plant Cell. 2004, 16 (6): 1446-1465. 10.1105/tpc.020297.View ArticleGoogle Scholar
- Wagner A, Ralph J, Akiyama T, Flint H, Phillips L, Torr K, Nanayakkara B, Kiri LT: Exploring lignification in conifers by silencing hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase in Pinus radiata. Proc Natl Aca Sci USA. 2007, 104 (28): 11856-11861. 10.1073/pnas.0701428104.View ArticleGoogle Scholar
- Comino C, Lanteri S, Portis E, Acquadro A, Romani A, Hehn A, Larbat R, Bourgaud F: Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L. BMC Plant Biol. 2007, 20 (7): 14-View ArticleGoogle Scholar
- Kim B-G, Lee ET, Ahn J-H: Characterization of hydroxycinnamoyl-coenzyme A shikimate hydroxycinnamoyltransferase from Populus euramericana. J Kor Soc Appl Biol Chem. 2011, 54 (2): 817-821.View ArticleGoogle Scholar
- St Pierre B, De Luca V: Evolution of acyltransferase genes: origin and diversification of the BAHD superfamily of acyltransferases involved in secondary metabolism. Recent Advances in Phytochemistry Vol 34. Evolution of Metabolic Pathways. Edited by: Romeo JT, Ibrahim R, Varin L, De Luca V. 2000, 285-315. Oxford: Elsevier Science LtdGoogle Scholar
- Horinouchi S: Combinatorial biosynthesis of non-bacterial and unnatural flavonoids, stilbenoids and curcuminoids by microorganisms. J Antibiot. 2008, 6 (12): 709-728.View ArticleGoogle Scholar
- Ikeda M: Towards bacterial strains overproducing L-tryptophan and other aromatics by metabolic engineering. Appl Microbiol Biotechnol. 2006, 69 (6): 615-626. 10.1007/s00253-005-0252-y.View ArticleGoogle Scholar
- Gosset G: Production of aromatic compounds in bacteria. Curr Opin Biotech. 2009, 20 (6): 651-658. 10.1016/j.copbio.2009.09.012.View ArticleGoogle Scholar
- Dixon RA, Paiva NL: Stress-induced phenylpropanoid metabolism. Plant Cell. 1995, 7 (7): 1085-1097.View ArticleGoogle Scholar
- LeeY J, Jeon Y, Lee JS, Kim B-G, Lee CH, Ahn J-H: Enzymatic synthesis of phenolic CoAs using 4-coumarate:coenzyme A ligase (4CL) from rice. Bull Kor Chem Soc. 2007, 28 (3): 365-366.View ArticleGoogle Scholar
- Diaz E, Ferrández A, Prieto MA, Garcia JL: Biodegradation of aromatic compounds by Escherichia coli. Microbiol Mol Biol Rev. 2001, 65 (4): 523-569. 10.1128/MMBR.65.4.523-569.2001.View ArticleGoogle Scholar
- Jaiswal R, Patras MA, Eravuchira PJ, Kuhnert N: Profile and characterization of the chlorogenic acids in green robusta coffee beans by LC-MSn: Identification of seven new classes of compounds. J Agric Food Chem. 2010, 58 (15): 8722-8737. 10.1021/jf1014457.View ArticleGoogle Scholar
- Draths KM, Knop DR, Frost JW: Shikimic acid and quinic acid: replacing isolation from plant sources with recombinant microbial biocatalysis. J Am Chem Soc. 1999, 121 (7): 1603-1604. 10.1021/ja9830243.View ArticleGoogle Scholar
- Escalante A, Calderón R, Valdivia A, de Anda R, Hernánde G, Ramirez OT, Gosset G, Bolivar F: Metabolic engineering for the production of shikimic acid in an evolved Esherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system. Microbial Cell Fact. 2010, 9: 21-33. 10.1186/1475-2859-9-21.View ArticleGoogle Scholar
- Krämer M, Bongaerts J, Bovenberg R, Kremer S, Müller U, Orf S, Wubbolts M, Raeven L: Metabolic engineering for microbial production of shikimic acid. Met Eng. 2003, 5 (4): 277-283. 10.1016/j.ymben.2003.09.001.View ArticleGoogle Scholar
- Niggeweg R, Michael AJ, Martin C: Engineering plants with increased levels of the antioxidant chlorogenic acid. Nat Biotech. 2004, 22 (4): 746-754.View ArticleGoogle Scholar
- Zhang H, Stephanopoulos G: Engineering E. coli for caffeic acid biosynthesis from renewable sugar. Appl Microbiol Biotechnol. 2012, 10.1007/s00253-012-4544-8.Google Scholar
- Lindner HA, Nadeau G, Matte A, Michel G, Ménard R, Cygler M: Site-directed mutagenesis of the active site region in the quinate/shimate 5-dehydrogenase YdiB of Escherichia coli. J Biol Chem. 2005, 280 (8): 7162-7169. 10.1074/jbc.M412028200.View ArticleGoogle Scholar
- Sung SH, Kim BG, Ahn J-H: Optimization of rhamnetin production in Escherichia coli. J Microbio Biotech. 2011, 21 (8): 854-857. 10.4014/jmb.1104.04048.View ArticleGoogle Scholar
- Zheng W, Clifford MN: Profiling the chlorogenic acids of sweet potato (Ipomoea batatas) from China. Food Chem. 2010, 106 (1): 147-152.View ArticleGoogle Scholar
- Garcia-Conesa MT, Plumb GW, Waldron KW, Ralph J, Williamson G: Ferulic acid dehydrodimers from wheat bran: isolation, purification and antioxidant properties of 8-O-4-diferulic acid. Redox Rep. 1997, 3 (5–6): 319-323.Google Scholar
- Berner M, Krug D, Bihlmaier C, Vente A, Müller R, Bechthold A: Genes and enzymes involved in caffeic acid biosynthesis in the actinomycete Saccharothix espanaensis. J Bact. 2006, 188 (7): 2666-2673. 10.1128/JB.188.7.2666-2673.2006.View ArticleGoogle Scholar
- Juminaga D, Baidoo EE, Redding-Johanson AM, Batth TS, Burd H, Mukhopadhyay A, Petzold CJ, Keasling JD: Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012, 78 (1): 89-98. 10.1128/AEM.06017-11.View ArticleGoogle Scholar
- Michel G, Roszak AW, Sauvé V, Maclean J, Matte A, Coggins JR, Cygler M, Lapthorn AJ: Structure of shikimate dehydrogenase AroE and its paralog YdiB. J Biol Chem. 2003, 278 (21): 19463-19472. 10.1074/jbc.M300794200.View ArticleGoogle Scholar
- Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA. 2000, 97 (12): 6640-6645. 10.1073/pnas.120163297.View ArticleGoogle Scholar
- Yoon J-A, Kim B-G, Lee WJ, Lim Y, Chong Y, Ahn J-H: Production of a novel quercetin glycoside through metabolic engineering of Escherichia coli. Appl Env Microbiol. 2012, 78 (12): 4256-4262. 10.1128/AEM.00275-12.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.