Table 1 Plasmids, Escherichia coli strains, and primers used in this study
Plasmids or E. coli strains or Primers | Relevant properties or genetic marker | Source or reference |
|---|---|---|
Plasmids | ||
pCYCDuet | P15A ori, Cmr | Novagen |
pCDFDuet | CloDE13 ori, Strr | Novagen |
pETDuet | f1 ori, Ampr | Novagen |
pA-NtHST-Os4CL | pACYCDDuet carrying NtHST from N. tobacco and 4CL from O. sativa | This study |
pC-NtHST-Os4CL | pCDFDuet carrying NtHST from N. tobacco and 4CL from O. sativa | This study |
pE-NtHST-Os4CL | pETDuet carrying NtHST from N. tobacco and 4CL from O. sativa | This study |
pC-NtHQT-Os4CL | pCDFDuet carrying NtHQT from N. tobacco and 4CL from O. sativa | This study |
pA-EcydiB | pACYC carrying ydiB from E. coli | This study |
Strains | Â | Â |
BL21 (DE3) | F-ompT hsdS B (rB- mB-) gal dcm lon (DE3) | Novagen |
B-100 | BaroD carrying pCDF-Duet | This study |
B-101 | BaroD carrying pC-NtHQT-Os4CL | This study |
B-102 | BaroD carrying pC-NtHQT-Os4CL and pA-EcydiB | This study |
BaroD | BL21(DE3) ΔaroD::FRT-kanR-FRT | This study |
BaroK | BL21(DE3) ΔaroK::FRT-kanR-FRT | This study |
BaroL | BL21(DE3) ΔaroL::FRT-kanR-FRT | This study |
BaroKL | BL21(DE3) ΔaroK::FRT ΔaroL::FRT-kanR-FRT | This study |
Primers | Â | Â |
NtHST-F1 | AACATATG AAGATCGAAGTGAAAGAAT (NdeI site is underlined) | |
NtHST-R2 | AACTCGAG TCAAAAGTCATACAAGAACTTC (XhoI site is underlined) | |
NtHQT-F | AAGATATC CATGGGAAGTGAAAAAATGATGA (EcoRV site is underlined) | |
NtHQT-R | AAGGTACC TCAAAATTCATACAAATACTT (KpnI site is underlined) | |
ydiB-F | ATGAATTC GATGGATGTTACCGCAAAATAC (EcoR I site is underlined) | |
ydiB-R | CATGCGGCCGC TCAGGCACCGAACCCCATG (Not I site is underlined) | |
aroK-F | gctgtcttttttacgctaatcttacccggtgatttatcgccagagcggtgaattaaccctcactaaagggcg | |
aroK-R | cccgcagacgagtgtatataaagccagaattagttgctttccagcatgtgtaatacgactcactatagggctc | |
aroL-F | atgacaccggctttcgccgcattgcgacctattggggaaaacccacgatgaattaaccctcactaaagggcg | |
aroL-R | gatgaacgttaagtataggcgctcgaaaatcaacaattgatcgtctgtgctaatacgactcactatagggctc | |
aroD-F | tggggttcggtgcctgacaggctgaccgcgtgcagaaagggtaaaaaatgaattaaccctcactaaagggcg | |
aroD-R | gggaggatattcccgccgaaatattattgcttatgcctgatgtaaaatagttaatacgactcactatagggctc | |
aroK-check-F | cgctgcctgcgttccatgat | |
aroL-check-F | cgcggagctggagaagtggt | |
aroK or aroL check-R | taatacgactcactatagggctc | |
aroD-check-F | ggcaaggggctgaacagttc | |
aroD-check-R | gggaggatattcccgccgaa | |